R J Deshaies

Summary

Affiliation: California Institute of Technology
Country: USA

Publications

  1. ncbi request reprint Phosphorylation and proteolysis: partners in the regulation of cell division in budding yeast
    R J Deshaies
    Division of Biology, California Institute of Technology, Pasadena California, 91125 USA
    Curr Opin Genet Dev 7:7-16. 1997
  2. ncbi request reprint SCF and Cullin/Ring H2-based ubiquitin ligases
    R J Deshaies
    Department of Biology, California Institute of Technology, Pasadena 91125, USA
    Annu Rev Cell Dev Biol 15:435-67. 1999
  3. pmc Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34
    J H Seol
    Division of Biology, California Institute of Technology, Pasadena, California 91125, USA
    Genes Dev 13:1614-26. 1999
  4. pmc Human CUL1 forms an evolutionarily conserved ubiquitin ligase complex (SCF) with SKP1 and an F-box protein
    S A Lyapina
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 95:7451-6. 1998
  5. ncbi request reprint Net1 stimulates RNA polymerase I transcription and regulates nucleolar structure independently of controlling mitotic exit
    W Shou
    Division of Biology, California Institute of Technology, Pasadena 91125, USA
    Mol Cell 8:45-55. 2001
  6. ncbi request reprint Selective degradation of ubiquitinated Sic1 by purified 26S proteasome yields active S phase cyclin-Cdk
    R Verma
    Howard Hughes Medical Institute, Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Mol Cell 8:439-48. 2001
  7. ncbi request reprint A complex of Cdc4p, Skp1p, and Cdc53p/cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Sic1p
    R M Feldman
    Division of Biology, California Institute of Technology, Pasadena 91125, USA
    Cell 91:221-30. 1997
  8. ncbi request reprint Phosphorylation of Sic1p by G1 Cdk required for its degradation and entry into S phase
    R Verma
    Division of Biology, Box 156 29, California Institute of Technology, Pasadena, CA 91125, USA
    Science 278:455-60. 1997
  9. ncbi request reprint Promotion of NEDD-CUL1 conjugate cleavage by COP9 signalosome
    S Lyapina
    Department of Biology, Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 91125, USA
    Science 292:1382-5. 2001
  10. pmc Protein kinase Cdc15 activates the Dbf2-Mob1 kinase complex
    A S Mah
    Division of Biology and Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 98:7325-30. 2001

Collaborators

Detail Information

Publications18

  1. ncbi request reprint Phosphorylation and proteolysis: partners in the regulation of cell division in budding yeast
    R J Deshaies
    Division of Biology, California Institute of Technology, Pasadena California, 91125 USA
    Curr Opin Genet Dev 7:7-16. 1997
    ..Thus, stable oscillations of chromosome replication and segregation in budding yeast are propagated by the interplay between protein phosphorylation and protein degradation...
  2. ncbi request reprint SCF and Cullin/Ring H2-based ubiquitin ligases
    R J Deshaies
    Department of Biology, California Institute of Technology, Pasadena 91125, USA
    Annu Rev Cell Dev Biol 15:435-67. 1999
    ..This review is focused on a conserved ubiquitin ligase complex known as SCF that plays a key role in marking a variety of regulatory proteins for destruction by the 26S proteasome...
  3. pmc Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34
    J H Seol
    Division of Biology, California Institute of Technology, Pasadena, California 91125, USA
    Genes Dev 13:1614-26. 1999
    ..We conclude that Cdc53/Hrt1 comprise a highly conserved module that serves as the functional core of a broad variety of heteromeric ubiquitin ligases...
  4. pmc Human CUL1 forms an evolutionarily conserved ubiquitin ligase complex (SCF) with SKP1 and an F-box protein
    S A Lyapina
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 95:7451-6. 1998
    ....
  5. ncbi request reprint Net1 stimulates RNA polymerase I transcription and regulates nucleolar structure independently of controlling mitotic exit
    W Shou
    Division of Biology, California Institute of Technology, Pasadena 91125, USA
    Mol Cell 8:45-55. 2001
    ..The independent functions of Net1 link a key event in the cell cycle to nucleolar processes that are fundamental to cell growth...
  6. ncbi request reprint Selective degradation of ubiquitinated Sic1 by purified 26S proteasome yields active S phase cyclin-Cdk
    R Verma
    Howard Hughes Medical Institute, Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Mol Cell 8:439-48. 2001
    ..Activation of S-Cdk reported herein represents a complete reconstitution of the regulatory switch underlying the G1/S transition in budding yeast...
  7. ncbi request reprint A complex of Cdc4p, Skp1p, and Cdc53p/cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Sic1p
    R M Feldman
    Division of Biology, California Institute of Technology, Pasadena 91125, USA
    Cell 91:221-30. 1997
    ..Taken together, these data illuminate the molecular basis for the G1/S transition in budding yeast and suggest a general mechanism for phosphorylation-targeted ubiquitination in eukaryotes...
  8. ncbi request reprint Phosphorylation of Sic1p by G1 Cdk required for its degradation and entry into S phase
    R Verma
    Division of Biology, Box 156 29, California Institute of Technology, Pasadena, CA 91125, USA
    Science 278:455-60. 1997
    ..These data suggest a molecular model of how phosphorylation and proteolysis cooperate to bring about the G1/S transition in budding yeast...
  9. ncbi request reprint Promotion of NEDD-CUL1 conjugate cleavage by COP9 signalosome
    S Lyapina
    Department of Biology, Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 91125, USA
    Science 292:1382-5. 2001
    ....
  10. pmc Protein kinase Cdc15 activates the Dbf2-Mob1 kinase complex
    A S Mah
    Division of Biology and Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 98:7325-30. 2001
    ..We propose that Mob1 promotes this activation process by enabling Cdc15 to phosphorylate the critical Ser-374 and Thr-544 phosphoacceptor sites of Dbf2...
  11. pmc Negative regulation of Gcn4 and Msn2 transcription factors by Srb10 cyclin-dependent kinase
    Y Chi
    Division of Biology, California Institute of Technology, Pasadena 91125, USA
    Genes Dev 15:1078-92. 2001
    ..Simultaneous down-regulation of both transcriptional regulatory proteins and RNA polymerase may enhance the potency and specificity of transcriptional inhibition by Srb10...
  12. ncbi request reprint Multisite phosphorylation and the countdown to S phase
    R J Deshaies
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Cell 107:819-22. 2001
    ..This numerical wizardry suggests how analog inputs can be rectified to digital outputs. Unraveling the counting mechanism promises to generate new insights into the architecture of protein nanoprocessors...
  13. ncbi request reprint Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly
    J H Seol
    Division of Biology and Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California 91125, USA
    Nat Cell Biol 3:384-91. 2001
    ..Thus, our results provide a distinct view of the interactions that link proteins into a comprehensive cellular network...
  14. pmc Cks1 is required for G(1) cyclin-cyclin-dependent kinase activity in budding yeast
    G J Reynard
    Division of Biology, California Institute of Technology, Pasadena, California 91125, USA
    Mol Cell Biol 20:5858-64. 2000
    ..The role of Cks1 in promoting G(1) cyclin-CDK protein kinase activity both in vitro and in vivo provides a simple molecular rationale for the essential role of CKS1 in progression through G(1) phase in budding yeast...
  15. ncbi request reprint Exit from mitosis is triggered by Tem1-dependent release of the protein phosphatase Cdc14 from nucleolar RENT complex
    W Shou
    Division of Biology, California Institute of Technology, Pasadena, California 91125, USA
    Cell 97:233-44. 1999
    ..Nucleolar sequestration may be a general mechanism for the regulation of diverse biological processes...
  16. ncbi request reprint Assaying degradation and deubiquitination of a ubiquitinated substrate by purified 26S proteasomes
    Rati Verma
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    Methods Enzymol 398:391-9. 2005
    ..Once targeted, the substrate is deubiquitinated and degraded by the 26S proteasome. This chapter describes assays that monitor ATP- and ubiquitin-dependent proteolysis of the S-Cdk inhibitor Sic1...
  17. pmc SIC1 is ubiquitinated in vitro by a pathway that requires CDC4, CDC34, and cyclin/CDK activities
    R Verma
    Division of Biology, California Institute of Technology, Pasadena 91125, USA
    Mol Biol Cell 8:1427-37. 1997
    ..The complementary C-terminal segment of SIC1 binds to the S-phase cyclin CLB5, indicating a modular structure for SIC1...
  18. ncbi request reprint The self-destructive personality of a cell cycle in transition
    R J Deshaies
    Division of Biology, California Institute of Technology, Pasadena 91125, USA
    Curr Opin Cell Biol 7:781-9. 1995
    ..Genetic and biochemical dissections of these extremely specific and well regulated destruction pathways are providing fundamental insights into the mechanisms of control of the cell division cycle...