Alexei A Aravin
Affiliation: California Institute of Technology
Lisitskaya L, Aravin A, Kulbachinskiy A. DNA interference and beyond: structure and functions of prokaryotic Argonaute proteins. Nat Commun. 2018;9:5165 pubmed publisher
..Here, we review available structural and biochemical data on pAgos and discuss their possible functions in host defense and other genetic processes in prokaryotic cells. ..
Le Thomas A, Marinov G, Aravin A. A transgenerational process defines piRNA biogenesis in Drosophila virilis. Cell Rep. 2014;8:1617-1623 pubmed publisher
..The inherited piRNAs enhance piRNA biogenesis through the installment of H3K9me3 on piRNA clusters. ..
Liu Y, Esyunina D, Olovnikov I, Teplova M, Kulbachinskiy A, Aravin A, et al
. Accommodation of Helical Imperfections in Rhodobacter sphaeroides Argonaute Ternary Complexes with Guide RNA and Target DNA. Cell Rep. 2018;24:453-462 pubmed publisher
..Our results reveal plasticity in the ability of Ago proteins to accommodate helical imperfections, show how this might affect the efficiency of RNA silencing, and suggest a potential mechanism for guide release and Ago recycling. ..
Hur J, Olovnikov I, Aravin A. Prokaryotic Argonautes defend genomes against invasive DNA. Trends Biochem Sci. 2014;39:257-9 pubmed publisher
..Here we highlight two recent studies that discover the functions of prokaryotic Argonautes in defence against exogenous DNA. ..
Huang X, Fejes Toth K, Aravin A. piRNA Biogenesis in Drosophila melanogaster. Trends Genet. 2017;33:882-894 pubmed publisher
..We finish by discussing current models to the still unresolved question of how piRNA precursors are selected and channeled into the processing machinery. ..
Le Thomas A, Stuwe E, Li S, Du J, Marinov G, Rozhkov N, et al
. Transgenerationally inherited piRNAs trigger piRNA biogenesis by changing the chromatin of piRNA clusters and inducing precursor processing. Genes Dev. 2014;28:1667-80 pubmed publisher
Manakov S, Pezic D, Marinov G, Pastor W, Sachidanandam R, Aravin A. MIWI2 and MILI Have Differential Effects on piRNA Biogenesis and DNA Methylation. Cell Rep. 2015;12:1234-43 pubmed publisher
..MILI is responsible for DNA methylation of a larger subset of TE families than MIWI2 is, suggesting that the proteins have independent roles in establishing DNA methylation patterns. ..
Webster A, Li S, Hur J, Wachsmuth M, Bois J, Perkins E, et al
. Aub and Ago3 Are Recruited to Nuage through Two Mechanisms to Form a Ping-Pong Complex Assembled by Krimper. Mol Cell. 2015;59:564-75 pubmed publisher
..Our study reveals a multi-step process responsible for the assembly and function of nuage complexes in piRNA-guided transposon repression. ..