Gary Wessel

Summary

Affiliation: Brown University
Country: USA

Publications

  1. ncbi request reprint A molecular analysis of hyalin--a substrate for cell adhesion in the hyaline layer of the sea urchin embryo
    G M Wessel
    Department of Molecular and Cell Biology and Biochemistry, Brown University, Providence, Rhode Island 02912, USA
    Dev Biol 193:115-26. 1998
  2. ncbi request reprint Cortical granule translocation is microfilament mediated and linked to meiotic maturation in the sea urchin oocyte
    Gary M Wessel
    Department of Molecular and Cell Biology and Biochemistry, 69 Brown Street, Box G, Brown University, Providence, RI 02912, USA
    Development 129:4315-25. 2002
  3. pmc Cell surface changes in the egg at fertilization
    Gary M Wessel
    Department of Molecular and Cellular Biology, Brown University, Providence, Rhode Island 02912, USA
    Mol Reprod Dev 76:942-53. 2009
  4. doi request reprint Use of sea stars to study basic reproductive processes
    Gary M Wessel
    Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI 02912, USA
    Syst Biol Reprod Med 56:236-45. 2010
  5. ncbi request reprint SFE1, a constituent of the fertilization envelope in the sea urchin is made by oocytes and contains low-density lipoprotein-receptor-like repeats
    G M Wessel
    Department of Molecular and Cell Biology, Brown University, Providence, Rhode Island 02912, USA
    Biol Reprod 63:1706-12. 2000
  6. ncbi request reprint Direct molecular interaction of a conserved yolk granule protein in sea urchins
    G M Wessel
    Department of Molecular and Cell Biology and Biochemistry, Brown University, Providence, RI 02912, USA
    Dev Growth Differ 42:507-17. 2000
  7. ncbi request reprint How to grow a gut: ontogeny of the endoderm in the sea urchin embryo
    G M Wessel
    Department of Molecular and Cell Biology and Biochemistry, Brown University, Providence, Rhode Island 02912, USA
    Bioessays 21:459-71. 1999
  8. ncbi request reprint Germ line determinants are not localized early in sea urchin development, but do accumulate in the small micromere lineage
    Celina E Juliano
    Department of Molecular and Cell Biology and Biochemistry, Brown University, 69 Brown Street, Providence, RI 02912, USA
    Dev Biol 300:406-15. 2006
  9. ncbi request reprint Genes involved in the RNA interference pathway are differentially expressed during sea urchin development
    Jia L Song
    Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, Rhode Island 02912, USA
    Dev Dyn 236:3180-90. 2007
  10. pmc Small micromeres contribute to the germline in the sea urchin
    Mamiko Yajima
    MCB Department, Brown University, 185 Meeting Street, BOX GL173, Providence, RI 02912, USA
    Development 138:237-43. 2011

Research Grants

  1. Cell Surface Changes During the Egg-to-Embryo Transition
    Gary Wessel; Fiscal Year: 2009
  2. Cell Surface Changes During the Egg-to-Embryo Transition
    Gary M Wessel; Fiscal Year: 2010
  3. Cell Surface Changes During the Egg-to-Embryo Transition
    Gary Wessel; Fiscal Year: 2009
  4. Cortical Granule Regulation and Function
    Gary Wessel; Fiscal Year: 2005
  5. CONFOCAL MICROSCOPE
    Gary Wessel; Fiscal Year: 2003
  6. BIOGENESIS OF CORTICAL GRANULES
    Gary Wessel; Fiscal Year: 2000
  7. Cell Surface Changes During the Egg-to-Embryo Transition
    Gary M Wessel; Fiscal Year: 2010

Collaborators

Detail Information

Publications48

  1. ncbi request reprint A molecular analysis of hyalin--a substrate for cell adhesion in the hyaline layer of the sea urchin embryo
    G M Wessel
    Department of Molecular and Cell Biology and Biochemistry, Brown University, Providence, Rhode Island 02912, USA
    Dev Biol 193:115-26. 1998
    ..These data help explain some of the classic functions ascribed to the hyalin protein in early development and now enable investigators to focus on the mechanisms of cell interactions with the hyaline layer...
  2. ncbi request reprint Cortical granule translocation is microfilament mediated and linked to meiotic maturation in the sea urchin oocyte
    Gary M Wessel
    Department of Molecular and Cell Biology and Biochemistry, 69 Brown Street, Box G, Brown University, Providence, RI 02912, USA
    Development 129:4315-25. 2002
    ..We conclude from these studies that maturation promoting factor (MPF) activation stimulates vesicle association with microfilaments, and is a key regulatory step in the coordinated translocation of cortical granules to the egg cortex...
  3. pmc Cell surface changes in the egg at fertilization
    Gary M Wessel
    Department of Molecular and Cellular Biology, Brown University, Providence, Rhode Island 02912, USA
    Mol Reprod Dev 76:942-53. 2009
    ..A recent conference in honor of his contributions stimulated this overview. We highlight the major cell surface changes that occur in echinoderms, one of Just's preferred research organisms...
  4. doi request reprint Use of sea stars to study basic reproductive processes
    Gary M Wessel
    Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI 02912, USA
    Syst Biol Reprod Med 56:236-45. 2010
    ..This review will highlight the fundamental protocols to enable a new investigator to perform an array of approaches on this organism, including oocyte isolation, microinjection, and even single cell quantitative PCR...
  5. ncbi request reprint SFE1, a constituent of the fertilization envelope in the sea urchin is made by oocytes and contains low-density lipoprotein-receptor-like repeats
    G M Wessel
    Department of Molecular and Cell Biology, Brown University, Providence, Rhode Island 02912, USA
    Biol Reprod 63:1706-12. 2000
    ....
  6. ncbi request reprint Direct molecular interaction of a conserved yolk granule protein in sea urchins
    G M Wessel
    Department of Molecular and Cell Biology and Biochemistry, Brown University, Providence, RI 02912, USA
    Dev Growth Differ 42:507-17. 2000
    ..It is hypothesized that YP30 participates in the packaging and storage of major yolk protein during oogenesis, or in the utilization of the major yolk protein in development...
  7. ncbi request reprint How to grow a gut: ontogeny of the endoderm in the sea urchin embryo
    G M Wessel
    Department of Molecular and Cell Biology and Biochemistry, Brown University, Providence, Rhode Island 02912, USA
    Bioessays 21:459-71. 1999
    ..Recent progress at the molecular level should soon allow us to explain the seminal experimental observations made in this embryo over a hundred years ago...
  8. ncbi request reprint Germ line determinants are not localized early in sea urchin development, but do accumulate in the small micromere lineage
    Celina E Juliano
    Department of Molecular and Cell Biology and Biochemistry, Brown University, 69 Brown Street, Providence, RI 02912, USA
    Dev Biol 300:406-15. 2006
    ..Furthermore, accumulation of germ line determinants selectively in small micromere descendents supports the hypothesis that these cells contribute to the germ line...
  9. ncbi request reprint Genes involved in the RNA interference pathway are differentially expressed during sea urchin development
    Jia L Song
    Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, Rhode Island 02912, USA
    Dev Dyn 236:3180-90. 2007
    ..This study elucidated that genes involved in the RNAi silencing pathway have dynamic expression and, thus, may have regulatory roles during germ cell development and embryogenesis...
  10. pmc Small micromeres contribute to the germline in the sea urchin
    Mamiko Yajima
    MCB Department, Brown University, 185 Meeting Street, BOX GL173, Providence, RI 02912, USA
    Development 138:237-43. 2011
    ..These results suggest that germline specification depends on the small micromeres, either directly as lineage products, or indirectly by signaling mechanisms emanating from the small micromeres or their descendants...
  11. ncbi request reprint Selective expression of a sec1/munc18 member in sea urchin eggs and embryos
    Mariana Leguia
    Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, 69 Brown Street, Providence, RI 02912, USA
    Gene Expr Patterns 4:645-57. 2004
    ..These results will be instrumental in the future to functionally test the SNARE regulators associated with multiple membrane fusion events...
  12. ncbi request reprint Proteolytic cleavage of the cell surface protein p160 is required for detachment of the fertilization envelope in the sea urchin
    Sheila A Haley
    Department of Molecular and Cellular Biology and Biochemistry, Brown University, Providence, RI 02912, USA
    Dev Biol 272:191-202. 2004
    ..Thus, p160 is a key candidate for a vitelline-layer linker protein, the selective proteolysis of which functions in the block to polyspermy in the sea urchin egg...
  13. ncbi request reprint Major components of a sea urchin block to polyspermy are structurally and functionally conserved
    Julian L Wong
    Department of Molecular Biology, Cellular Biology, and Biochemistry, Box G J4, Brown University, Providence, RI 02912, USA
    Evol Dev 6:134-53. 2004
    ....
  14. pmc Extracellular matrix modifications at fertilization: regulation of dityrosine crosslinking by transamidation
    Julian L Wong
    Department of Molecular Biology, Brown University, Providence, RI 02912, USA
    Development 136:1835-47. 2009
    ..Together, these results emphasize how transglutaminases can coordinate the activities of other enzymes during extracellular matrix transmogrifications...
  15. pmc Rendezvin: An essential gene encoding independent, differentially secreted egg proteins that organize the fertilization envelope proteome after self-association
    Julian L Wong
    Department of Molecular Biology, Cellular Biology, and Biochemistry, Brown University, Providence, RI 02912, USA
    Mol Biol Cell 17:5241-52. 2006
    ..These distinctly trafficked variants reunite after cortical granule secretion at fertilization. Together, they help coordinate assembly of the functional fertilization envelope, whose proteome is now defined in full...
  16. ncbi request reprint How to make an egg: transcriptional regulation in oocytes
    Jia L Song
    Department of Molecular and Cell Biology and Biochemistry, Brown University, 69 Brown Street, Box G J4, Providence, RI 02912, USA
    Differentiation 73:1-17. 2005
    ..We propose that oogenesis is reliant on a dynamic gene regulatory network that includes oocyte-specific transcriptional regulators...
  17. ncbi request reprint Isolation of organelles and components from sea urchin eggs and embryos
    Gary M Wessel
    Department of Molecular and Cell Biology and Biochemistry, Brown University, Providence, Rhode Island 02912, USA
    Methods Cell Biol 74:491-522. 2004
  18. ncbi request reprint The oxidative burst at fertilization is dependent upon activation of the dual oxidase Udx1
    Julian L Wong
    Department of Molecular Biology, Cell Biology, and Biochemistry, Box G, Brown University, Providence, RI 02912 USA
    Dev Cell 7:801-14. 2004
    ..Phylogenetic analysis of the enzymatic modules in Udx1 suggests a potentially conserved role for the dual oxidase family in hydrogen peroxide production and regulation during fertilization...
  19. pmc A conserved germline multipotency program
    Celina E Juliano
    Department of Molecular and Cellular Biology, Brown University, Providence, RI 02912, USA
    Development 137:4113-26. 2010
    ..As we propose here, this conservation implies the existence of an underlying germline multipotency program in these cell types that has a previously underappreciated and conserved function in maintaining multipotency...
  20. ncbi request reprint Synaptotagmin I is involved in the regulation of cortical granule exocytosis in the sea urchin
    Mariana Leguia
    Department of Molecular and Cellular Biology and Biochemistry, Brown University, Providence, Rhode Island 02912, USA
    Mol Reprod Dev 73:895-905. 2006
    ..Identification of this same protein in other developmental stages also shown here will be important for interpreting stimulus-dependent secretory events for signaling throughout embryogenesis...
  21. pmc Vasa protein expression is restricted to the small micromeres of the sea urchin, but is inducible in other lineages early in development
    Ekaterina Voronina
    Providence Institute of Molecular Oogenesis, Department of Molecular Biology, Cell Biology and Biochemistry, 185 Meeting Street, Box G, Brown University, Providence, RI 02912, USA
    Dev Biol 314:276-86. 2008
    ....
  22. doi request reprint FRAP analysis of secretory granule lipids and proteins in the sea urchin egg
    Julian L Wong
    Department of Molecular Biology, Cellular Biology and Biochemistry, Brown University, Providence, RI, USA
    Methods Mol Biol 440:61-76. 2008
    ..Their large size (1.3-microm diameter), vast number, and ease of manipulation enable several unique approaches to study general secretion mechanisms...
  23. ncbi request reprint Free-radical crosslinking of specific proteins alters the function of the egg extracellular matrix at fertilization
    Julian L Wong
    Department of Molecular Biology, Cellular Biology, and Biochemistry, Box G L173, Brown University, Providence, RI 02912, USA
    Development 135:431-40. 2008
    ..Thus, specific free-radical chemistry is essential for establishing the embryonic microenvironment of early development...
  24. ncbi request reprint Regulatory contribution of heterotrimeric G-proteins to oocyte maturation in the sea urchin
    Ekaterina Voronina
    Department of Molecular and Cell Biology and Biochemistry, Brown University, 69 Brown Street, Providence, RI 02912, USA
    Mech Dev 121:247-59. 2004
    ..These studies provide a foundation to further test specific hypotheses of the G-protein mediated regulation of oocyte maturation, fertilization, and early development in the sea urchin...
  25. pmc Polycomb group gene expression in the sea urchin
    Eric A Gustafson
    Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, Rhode Island 02912, USA
    Dev Dyn 237:1851-61. 2008
    ..This finding is consistent with progressive cell differentiation in the embryo. Taken together, these results suggest that PcG genes are conserved in S. purpuratus and are actively expressed during early embryogenesis...
  26. pmc Nanos functions to maintain the fate of the small micromere lineage in the sea urchin embryo
    Celina E Juliano
    Department of Molecular and Cell Biology and Biochemistry, Brown University, 185 Meeting St, Providence, RI 02912, USA
    Dev Biol 337:220-32. 2010
    ..This work, in combination with other recent results in Ilyanassa and Platynereis dumerilii, suggests the presence of a conserved molecular program underlying both primordial germ cell and multipotent cell specification and maintenance...
  27. ncbi request reprint The histamine H1 receptor activates the nitric oxide pathway at fertilization
    Mariana Leguia
    Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, Rhode Island 02912, USA
    Mol Reprod Dev 73:1550-63. 2006
    ..Inhibition of suH(1)R function decreases the maintenance, but not the amplitude, of the Ca(2+) transient and suggests that it is an integral part of the overall pathway leading to egg activation at fertilization in sea urchins...
  28. ncbi request reprint In the beginning...animal fertilization and sea urchin development
    Elissa Briggs
    Department of Molecular Biology, Cell Biology and Biochemistry, Box G, 69 Brown Street, Brown University, Providence, RI 02912, USA
    Dev Biol 300:15-26. 2006
    ..I saw this envelope manifest when in contact with a very small number of spermatozoids (three or four, sometimes even one only)...
  29. ncbi request reprint Selective transport and packaging of the major yolk protein in the sea urchin
    Jacqueline M Brooks
    Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, RI 02912, USA
    Dev Biol 261:353-70. 2003
    ..In addition, these results demonstrate that the entire population of yolk platelets is competent to receive new yolk protein input, suggesting that they are all made simultaneously during oogenesis...
  30. pmc Regulated proteolysis by cortical granule serine protease 1 at fertilization
    Sheila A Haley
    Department of Molecular and Cell Biology and Biochemistry, Brown University, Providence, RI 02916, USA
    Mol Biol Cell 15:2084-92. 2004
    ..Thus, CGSP1 is proteolytically active only when secreted, due to the low pH of the cortical granules, and it has a small population of targets for cleavage within the cortical granules...
  31. doi request reprint Renovation of the egg extracellular matrix at fertilization
    Julian L Wong
    Department of Molecular Biology, Cellular Biology, and Biochemistry, Brown University, Providence, RI 02912, USA
    Int J Dev Biol 52:545-50. 2008
    ..Here we integrate the cellular and molecular events necessary to form this fertilization envelope within the first few minutes of a new embryo's life...
  32. ncbi request reprint Cyclin B synthesis is required for sea urchin oocyte maturation
    Ekaterina Voronina
    Department of Molecular and Cell Biology and Biochemistry, Brown University, Providence, RI 02912, USA
    Dev Biol 256:258-75. 2003
    ..We also find that cyclin A can functionally substitute for cyclin B in early embryos but not in oocytes. These studies provide a foundation for understanding the mechanism of meiotic maturation independent of the zygotic cell cycle...
  33. pmc Membrane hemifusion is a stable intermediate of exocytosis
    Julian L Wong
    Department of Molecular Biology, Cellular Biology, and Biochemistry, Brown University, Providence, RI 02912, USA
    Dev Cell 12:653-9. 2007
    ..Thus, these secretory granules are tethered to their target plasma membrane by a static, catalytic fusion complex that maintains a hemifused membrane intermediate...
  34. ncbi request reprint The regulation of oocyte maturation
    Ekaterina Voronina
    Department of Molecular and Cell Biology, Brown University, 69 Brown St, Providence, RI 02912, USA
    Curr Top Dev Biol 58:53-110. 2003
  35. pmc An evolutionary transition of Vasa regulation in echinoderms
    Celina E Juliano
    Department of Molecular and Cell Biology and Biochemistry, Brown University, Providence, RI 02912, USA
    Evol Dev 11:560-73. 2009
    ....
  36. ncbi request reprint Oogenesis: single cell development and differentiation
    Jia L Song
    Department of Molecular and Cellular Biology and Biochemistry, Box G, Brown University, Providence, RI 02912, USA
    Dev Biol 300:385-405. 2006
    ....
  37. ncbi request reprint The major yolk protein of sea urchins is endocytosed by a dynamin-dependent mechanism
    Jacqueline M Brooks
    Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, Rhode Island 02912, USA
    Biol Reprod 71:705-13. 2004
    ..Using this specific biochemical approach, we are able to separate distinct pathways of endocytosis during oogenesis and learn that dynamin-mediated endocytosis is responsible for MYP endocytosis but not fluid-phase uptake...
  38. ncbi request reprint The major yolk protein in sea urchins is a transferrin-like, iron binding protein
    Jacqueline M Brooks
    Department of Molecular and Cell Biology and Biochemistry, Division of Biology and Medicine, Brown University, Providence, RI 02912, USA
    Dev Biol 245:1-12. 2002
    ..We suggest that one of MYP's transferrin-like properties is to shuttle iron to developing germ cells...
  39. ncbi request reprint Defending the zygote: search for the ancestral animal block to polyspermy
    Julian L Wong
    Department of Molecular Biology, Cellular Biology, and Biochemistry, Brown University, Providence, Rhode Island 02912, USA
    Curr Top Dev Biol 72:1-151. 2006
    ..We also consider the evolution of alternative or radical techniques, including physiological polyspermy, with respect to our ability to describe a parsimonious guide to fertilization...
  40. ncbi request reprint betagamma subunits of heterotrimeric G-proteins contribute to Ca2+ release at fertilization in the sea urchin
    Ekaterina Voronina
    Department of Molecular and Cell Biology and Biochemistry, Brown University, 69 Brown Street, Providence, RI 02912, USA
    J Cell Sci 117:5995-6005. 2004
    ..We propose integration of the G-protein input into the framework of signaling at sea urchin fertilization...
  41. ncbi request reprint Activator of G-protein signaling in asymmetric cell divisions of the sea urchin embryo
    Ekaterina Voronina
    Department of Molecular and Cell Biology and Biochemistry, Brown University, Providence, Rhode Island 02912, USA
    Dev Growth Differ 48:549-57. 2006
    ..These results support the growing evidence that AGS is a universal regulator of asymmetric cell divisions in embryos...
  42. ncbi request reprint Obtaining and handling echinoderm oocytes
    Gary M Wessel
    Department of Molecular and Cell Biology and Biochemistry, Brown University, Providence, Rhode Island 02912, USA
    Methods Cell Biol 74:87-114. 2004
  43. pmc Cyclin D and cdk4 are required for normal development beyond the blastula stage in sea urchin embryos
    Jennifer C Moore
    Program in Molecular Biology and Biotechnology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Mol Cell Biol 22:4863-75. 2002
    ..These results suggest that in sea urchins, cyclin D and cdk4 are required for normal development and perhaps the patterning of the developing embryo, but may not be directly involved in regulating entry into the cell cycle...
  44. pmc The genome of the sea urchin Strongylocentrotus purpuratus
    Erica Sodergren
    Science 314:941-52. 2006
    ..This echinoderm genome provides an evolutionary outgroup for the chordates and yields insights into the evolution of deuterostomes...
  45. ncbi request reprint A Rho-signaling pathway mediates cortical granule translocation in the sea urchin oocyte
    Fernando Covián-Nares
    Instituto de Investigacion en Biologia Experimental, Facultad de Quimica, Universidad de Guanajuato, Col Noria Alta, Guanajuato, Gto 3600, A P 187, Mexico
    Mech Dev 121:225-35. 2004
    ..We conclude that Rho association with the cortical granules is a critical regulatory step in their translocation to the egg cortex...
  46. ncbi request reprint The invertebrate deuterostomes: an introduction to their phylogeny, reproduction, development, and genomics
    Charles A Ettensohn
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA
    Methods Cell Biol 74:1-13. 2004
  47. ncbi request reprint Lineage-specific expansions provide genomic complexity among sea urchin GTPases
    Wendy S Beane
    Department of Biology, Developmental, Cell and Molecular Group, Duke University, Box 91000, Durham, NC 27708, USA
    Dev Biol 300:165-79. 2006
    ..These findings suggest that lineage-specific expansions may be an important component of genomic evolution in signal transduction...
  48. ncbi request reprint Calcium-triggered membrane fusion proceeds independently of specific presynaptic proteins
    Joseph A Szule
    Department of Physiology and Biophysics, Cellular and Molecular Neurobiology Research Group, University of Calgary, Health Sciences Centre, Faculty of Medicine, Alberta T2N 4N1, Canada
    J Biol Chem 278:24251-4. 2003
    ..SNAREs, synaptotagmin, and their immediate binding partners are critical to exocytosis at a stage other than membrane fusion, although they may still influence the triggered steps...

Research Grants16

  1. Cell Surface Changes During the Egg-to-Embryo Transition
    Gary Wessel; Fiscal Year: 2009
    ..Our results will have particular significance to clinical IVF predictions in human reproductive health. ..
  2. Cell Surface Changes During the Egg-to-Embryo Transition
    Gary M Wessel; Fiscal Year: 2010
    ..Our results will have particular significance to clinical IVF predictions in human reproductive health. ..
  3. Cell Surface Changes During the Egg-to-Embryo Transition
    Gary Wessel; Fiscal Year: 2009
    ..Our results will have particular significance to clinical IVF predictions in human reproductive health. ..
  4. Cortical Granule Regulation and Function
    Gary Wessel; Fiscal Year: 2005
    ..We will focus on proteins that interact with SNARE homologues and of rab 3, and address both the mechanism for stimulating exocytosis, as well as the molecular clamp that blocks exocytosis until fertilization. ..
  5. CONFOCAL MICROSCOPE
    Gary Wessel; Fiscal Year: 2003
    ..The requested instrument would significantly enhance the research productivity of the broad, interdisciplinary community of researchers described here, and greatly improve the biological image capabilities in the region. ..
  6. BIOGENESIS OF CORTICAL GRANULES
    Gary Wessel; Fiscal Year: 2000
    ..The hypothesis will be tested that the extracellular matrix derived from maternal stores is important in the stimulation of zygotic ECM synthesis. This study will include the characterization of several novel ECM molecules. ..
  7. Cell Surface Changes During the Egg-to-Embryo Transition
    Gary M Wessel; Fiscal Year: 2010
    ..Ourresultswillhaveparticular significancetoclinicalIVFpredictionsinhumanreproductivehealth. ..