Affiliation: Brown University
Saffarini C, McDonnell Clark E, Amin A, Boekelheide K. A human fetal prostate xenograft model of developmental estrogenization. Int J Toxicol. 2015;34:119-28 pubmed publisher
Spade D, Hall S, Wilson S, Boekelheide K. Di-n-Butyl Phthalate Induces Multinucleated Germ Cells in the Rat Fetal Testis Through a Nonproliferative Mechanism. Biol Reprod. 2015;93:110 pubmed publisher
..Only one of 606 MNGs in BrdU-labeled sections had a strongly positive nucleus, confirming a nonproliferative mechanism of MNG formation, which is a degenerative process with the potential to adversely affect testis development. ..
Spade D, Bai C, Lambright C, Conley J, Boekelheide K, Gray L. Validation of an automated counting procedure for phthalate-induced testicular multinucleated germ cells. Toxicol Lett. 2018;290:55-61 pubmed publisher
..In conclusion, we have developed a reliable image analysis method that can be used to facilitate dose-response studies for the reproducible induction of MNGs by in utero phthalate exposure. ..
Dere E, Anderson L, Huse S, Spade D, McDonnell Clark E, Madnick S, et al
. Effects of continuous bisphenol A exposure from early gestation on 90 day old rat testes function and sperm molecular profiles: A CLARITY-BPA consortium study. Toxicol Appl Pharmacol. 2018;347:1-9 pubmed publisher
Boekelheide K, Fleming S, Allio T, Embree Ku M, Hall S, Johnson K, et al
. 2,5-hexanedione-induced testicular injury. Annu Rev Pharmacol Toxicol. 2003;43:125-47 pubmed
..The goal is to evaluate the technical and conceptual approaches used to assess 2,5-HD-induced testicular injury, to highlight unanswered questions, and to identify fruitful avenues of future research. ..
Boekelheide K, Andersen M. A mechanistic redefinition of adverse effects - a key step in the toxicity testing paradigm shift. ALTEX. 2010;27:243-52 pubmed
Vantangoli M, Wilson S, Madnick S, Huse S, Boekelheide K. Morphologic effects of estrogen stimulation on 3D MCF-7 microtissues. Toxicol Lett. 2016;248:1-8 pubmed publisher
..The use of a differentiated scaffold-free 3D culture system offers a unique opportunity to study the phenotypic and molecular changes associated with exposure to estrogenic compounds. ..
Dere E, Spade D, Hall S, Altemus A, Smith J, Phillips J, et al
. Identification of sperm mRNA biomarkers associated with testis injury during preclinical testing of pharmaceutical compounds. Toxicol Appl Pharmacol. 2017;320:1-7 pubmed publisher
..These results demonstrate that sperm mRNA levels are sensitive molecular indicators of testicular injury that can potentially be translated into a clinical setting. ..
Boekelheide K, Kleymenova E, Liu K, Swanson C, Gaido K. Dose-dependent effects on cell proliferation, seminiferous tubules, and male germ cells in the fetal rat testis following exposure to di(n-butyl) phthalate. Microsc Res Tech. 2009;72:629-38 pubmed publisher
..Cell proliferation and apoptosis are key processes regulating development of the testis, and alterations in these processes may underlie testicular dysgenesis...