Research Topics
| Betsy SutherlandSummaryAffiliation: Brookhaven National Laboratory Country: USA Publications
Research Grants
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Detail Information
Publications
Clustered DNA damages induced in isolated DNA and in human cells by low doses of ionizing radiationB M Sutherland
Biology Department, Brookhaven National Laboratory, Upton, NY 11973 5000, USA
Proc Natl Acad Sci U S A 97:103-8. 2000..We also show that even low doses (0.1-1 Gy) of high linear energy transfer ionizing radiation induce clustered damages in human cells...
Low levels of endogenous oxidative damage cluster levels in unirradiated viral and human DNAsBetsy M Sutherland
Biology Department, Brookhaven National Laboratory, Upton NY 11973 5000, USA
Free Radic Biol Med 35:495-503. 2003..In bacteriophage T7 DNA prepared using stringent conditions, the frequencies of these clusters were <1/Mbp. In DNA from unirradiated human 28SC monocytes, the levels of such clusters were, at most, a few per gigabase pair...- Clustered DNA damages induced in human hematopoietic cells by low doses of ionizing radiationBetsy M Sutherland
Biology Department, Brookhaven National Laboratory, Upton, NY 11973 5000, USA
J Radiat Res 43:S149-52. 2002..Studies are in progress to determine whether clusters can be produced by mechanisms other than ionizing radiation, as well as the levels of various cluster types formed by low and high LET radiation... - Evaluation of number average length analysis in quantifying double strand breaks in genomic DNAsBetsy M Sutherland
Department of Biology, Brookhaven National Laboratory, Building 463, 50 Bell Avenue, Upton, New York 11973 5000, USA
Biochemistry 42:3375-84. 2003..The use of this approach in quantifying DNA damages is illustrated for double strand breaks and damage clusters (e.g., OxyPurine clusters recognized by Escherichia coli Fpg protein) induced in T7 DNA by ionizing radiation... - Repair of cyclobutyl pyrimidine dimers in human skin: variability among normal humans in nucleotide excision and in photorepairBetsy M Sutherland
Biology Department, Building 463, Brookhaven National Laboratory, Upton, NY 11973 5000, USA
Photodermatol Photoimmunol Photomed 18:109-16. 2002..We met a similar problem of dimer induction by a PRL source. We designed and validated a PRL source of sufficient intensity to catalyse PR, but that did not induce CPD, and used it to measure photorepair in human skin... - Clustered DNA damages induced by x rays in human cellsBetsy M Sutherland
Biology Department, Brookhaven National Laboratory, Upton, New York 11973 5000, USA
Radiat Res 157:611-6. 2002..Non-DSB clustered damages comprise about 70% of the complex lesions produced in cells. The relative levels of specific cluster classes depend on the environment of the DNA... - Clustered DNA damages as dosemeters for ionising radiation exposure and biological responsesB M Sutherland
Biology Department, Brookhaven National Laboratory Upton, NY 11973 5000, USA
Radiat Prot Dosimetry 97:33-8. 2001..Since some clusters may not be repairable and may accumulate in cells, they might also be useful as integrating dosemeters of biological effects of radiation damage... - Frequencies and relative levels of clustered damages in DNA exposed to gamma rays in radioquenching vs. nonradioquenching conditionsB M Sutherland
Biology Department, Brookhaven National Laboratory, Upton, New York 11973 5000, USA
Environ Mol Mutagen 38:159-65. 2001..The ratio of the level of oxidized pyrimidine clusters to double-strand breaks in a DNA in radioquenching solution is similar to that obtained in DNA in human cells, also a radioquenching environment... - Clustered damages and total lesions induced in DNA by ionizing radiation: oxidized bases and strand breaksB M Sutherland
Biology Department, Building 463, Brookhaven National Laboratory, Upton, New York 11973 5000, USA
Biochemistry 39:8026-31. 2000..The data also show that each cluster results from a single radiation (track) event, and thus clusters will be formed at low as well as high radiation doses... - Endogenous DNA damage clusters in human skin, 3-D model, and cultured skin cellsPaula V Bennett
Biology Department, Brookhaven National Laboratory, Upton, NY 11973-5000, USA
Free Radic Biol Med 39:832-9. 2005..These results imply that the levels of endogenous clusters can be affected by the cells' external environment and their ability to deal with DNA damage... - High efficiency detection of bi-stranded abasic clusters in gamma-irradiated DNA by putrescineAlexandros G Georgakilas
Biology Department, Building 463, Brookhaven National Laboratory, Upton, NY 11973 5000, USA
Nucleic Acids Res 30:2800-8. 2002..Further comparison of PUTR and Nfo protein cleavage may provide useful information on abasic cluster levels and configurations induced by ionizing radiation... - Bistranded oxidized purine damage clusters: induced in DNA by long-wavelength ultraviolet (290-400 nm) radiation?Joon Myong Song
Biology Department, Brookhaven National Laboratory, Upton, New York 11973 5000, USA
Biochemistry 41:8683-8. 2002.... - Spectrum of complex DNA damages depends on the incident radiationMegumi Hada
Biology Department, Brookhaven National Laboratory, Upton, New York 11973-5000, USA
Radiat Res 165:223-30. 2006..The relative frequencies of DSBs compared to abasic and oxybase clusters were higher for the charged particles-including the high-energy, low-LET protons-than for the ionizing photons... - DNA damage quantitation by alkaline gel electrophoresisBetsy M Sutherland
Biology Department, Brookhaven National Laboratory, Upton, NY, USA
Methods Mol Biol 314:251-73. 2006.... 
Yields of clustered DNA damage induced by charged-particle radiations of similar kinetic energy per nucleon: LET dependence in different DNA microenvironmentsDeborah J Keszenman
Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA
Radiat Res 174:238-50. 2010..The yields of DSBs and non-DSB clusters as well as the damage spectra varied with LET and DNA milieu, suggesting the involvement of more than one mechanism in the formation of the different types of clustered damages...- Endogenous DNA damage clusters in human hematopoietic stem and progenitor cellsPaula Bennett
Biology Department, Brookhaven National Laboratory, Upton, NY 11973 5000, USA
Free Radic Biol Med 45:1352-9. 2008..These data suggest that in highly damaging environments, even normal DNA repair capacity can be overwhelmed, leaving highly repair-resistant clustered damages... - Expression of the E. coli fpg protein in CHO cells lowers endogenous oxypurine clustered damage levels and decreases accumulation of endogenous Hprt mutationsSunirmal Paul
Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA
Environ Mol Mutagen 47:311-9. 2006.... - Quantifying double-strand breaks and clustered damages in DNA by single-molecule laser fluorescence sizingElena M Filippova
Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA
Biophys J 84:1281-90. 2003..For a given size range of DNA molecules, single-molecule sizing is as or more sensitive than gel electrophoresis, and requires several orders-of-magnitude less DNA to determine damage levels... - Human abasic endonuclease action on multilesion abasic clusters: implications for radiation-induced biological damageBrigitte Paap
Biology Department, Brookhaven National Laboratory, Upton, NY 11973 5000, USA
Nucleic Acids Res 36:2717-27. 2008..The data suggest an underlying molecular mechanism for the higher levels of biological damage associated with agents producing complex clusters: the induction of highly repair-resistant bipolar clusters... - Saccharomyces cerevisiae-based system for studying clustered DNA damagesMario Moscariello
Brookhaven National Laboratory, Biology Department, Upton, NY 11973, USA
Radiat Environ Biophys 49:447-56. 2010..The system we describe might well be extended to assessing the repair of MDSs with different compositions, and to most of the complex DNA lesions induced by physical and chemical agents... - Proton-HZE-particle sequential dual-beam exposures increase anchorage-independent growth frequencies in primary human fibroblastsGuangming Zhou
Biology Department, Brookhaven National Laboratory, Upton, NY 11973-5000, USA
Radiat Res 166:488-94. 2006..However, irradiation of cells with either an iron or a titanium particle first followed by protons produced only additive levels... - Split-dose exposures versus dual ion exposure in human cell neoplastic transformationPaula V Bennett
Biology Department, Bldg 463, Brookhaven National Laboratory, Upton, NY 11973 5000, USA
Radiat Environ Biophys 46:119-23. 2007..Substantial increases in transformation levels were observed for all proton doses tested, including 1 cGy. These results point to the signal importance of protons in affecting the effect of space radiation on human cells... - Processing of bistranded abasic DNA clusters in gamma-irradiated human hematopoietic cellsAlexandros G Georgakilas
U PA Sch Med, Philadelphia
Nucleic Acids Res 32:5609-20. 2004..The existence of abasic clusters in 28SC monocytes, several days after irradiation suggests that they constitute persistent damages that could lead to mutation or cell killing... - Quantifying clustered DNA damage induction and repair by gel electrophoresis, electronic imaging and number average length analysisBetsy M Sutherland
U PA Sch Med, Philadelphia
Mutat Res 531:93-107. 2003..This review presents the theoretical basis, biochemical requirements and practical aspects of this approach, and shows examples of their applications in identification and quantitation of complex clustered damages... - Spontaneously occurring mutations in the cyclobutane pyrimidine dimer photolyase gene cause different sensitivities to ultraviolet-B in riceJun Hidema
Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai 980 8577, Japan
Plant J 43:57-67. 2005.... - Are endogenous clustered DNA damages induced in human cells?Paula V Bennett
Department of Biology, Brookhaven National Laboratory, Upton, NY 11973 5000, USA
Free Radic Biol Med 37:488-99. 2004..The absence of Nfo-detected endogenous clusters in any cells examined suggests that abasic clusters could be a signature of cellular ionizing radiation exposure...
Research Grants
- DNA Damage Clusters: Repair in Mammalian CellsBetsy Sutherland; Fiscal Year: 2004..Further, knowledge of conditions affecting formation of clustered damages, and cellular paths for dealing with them could allow the design of more effective radiation therapies. ..