F W Studier

Summary

Affiliation: Brookhaven National Laboratory
Country: USA

Publications

  1. ncbi Protein production by auto-induction in high density shaking cultures
    F William Studier
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA
    Protein Expr Purif 41:207-34. 2005
  2. ncbi Understanding the differences between genome sequences of Escherichia coli B strains REL606 and BL21(DE3) and comparison of the E. coli B and K-12 genomes
    F William Studier
    Biology Department, Brookhaven National Laboratory, PO Box 5000, Upton, NY 11973 5000, USA
    J Mol Biol 394:653-80. 2009
  3. ncbi Entry of bacteriophage T7 DNA into the cell and escape from host restriction
    B A Moffatt
    Biology Department, Brookhaven National Laboratory, Upton, New York 11973
    J Bacteriol 170:2095-105. 1988
  4. ncbi Multiple roles of T7 RNA polymerase and T7 lysozyme during bacteriophage T7 infection
    Xing Zhang
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973-5000, USA
    J Mol Biol 340:707-30. 2004
  5. ncbi Protein production and purification
    Susanne Gräslund
    Karolinska Institutet, Scheeles vag 2, 171 77 Stockholm, Sweden
    Nat Methods 5:135-46. 2008

Detail Information

Publications5

  1. ncbi Protein production by auto-induction in high density shaking cultures
    F William Studier
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA
    Protein Expr Purif 41:207-34. 2005
    ..Selenomethionine labeling was equally efficient in the commonly used methionine auxotroph B834(DE3) (found to be metE) or the prototroph BL21(DE3)...
  2. ncbi Understanding the differences between genome sequences of Escherichia coli B strains REL606 and BL21(DE3) and comparison of the E. coli B and K-12 genomes
    F William Studier
    Biology Department, Brookhaven National Laboratory, PO Box 5000, Upton, NY 11973 5000, USA
    J Mol Biol 394:653-80. 2009
    ..Complex deletions (dense clusters of small deletions and substitutions) apparently removed nonessential genes from approximately 30 sites in the basic genomes...
  3. ncbi Entry of bacteriophage T7 DNA into the cell and escape from host restriction
    B A Moffatt
    Biology Department, Brookhaven National Laboratory, Upton, New York 11973
    J Bacteriol 170:2095-105. 1988
    ..T7 mutants that lack all three strong early promoters A1, A2, and A3 could grow by using a secondary promoter...
  4. ncbi Multiple roles of T7 RNA polymerase and T7 lysozyme during bacteriophage T7 infection
    Xing Zhang
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973-5000, USA
    J Mol Biol 340:707-30. 2004
    ....
  5. ncbi Protein production and purification
    Susanne Gräslund
    Karolinska Institutet, Scheeles vag 2, 171 77 Stockholm, Sweden
    Nat Methods 5:135-46. 2008
    ..This review presents methods that could be applied at the outset of any project, a prioritized list of alternate strategies and a list of pitfalls that trip many new investigators...