J Shanklin

Summary

Affiliation: Brookhaven National Laboratory
Country: USA

Publications

  1. ncbi request reprint Desaturation and hydroxylation. Residues 148 and 324 of Arabidopsis FAD2, in addition to substrate chain length, exert a major influence in partitioning of catalytic specificity
    John A Broadwater
    Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA
    J Biol Chem 277:15613-20. 2002
  2. pmc Characterization and analysis of the cotton cyclopropane fatty acid synthase family and their contribution to cyclopropane fatty acid synthesis
    Xiao Hong Yu
    Department of Biochemistry and Cell Biology, Stony Brook University, NY, USA
    BMC Plant Biol 11:97. 2011
  3. pmc Identification of amino acid residues involved in substrate specificity of plant acyl-ACP thioesterases using a bioinformatics-guided approach
    Kimberly M Mayer
    Brookhaven National Laboratory, Department of Biology, Upton, NY 11973, USA
    BMC Plant Biol 7:1. 2007
  4. pmc Linking enzyme sequence to function using Conserved Property Difference Locator to identify and annotate positions likely to control specific functionality
    Kimberly M Mayer
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA
    BMC Bioinformatics 6:284. 2005
  5. ncbi request reprint Exploring the possibilities presented by protein engineering
    J Shanklin
    Department of Biology, Brookhaven National Laboratory, Upton, NY 11786, USA
    Curr Opin Plant Biol 3:243-8. 2000
  6. ncbi request reprint Overexpression and purification of the Escherichia coli inner membrane enzyme acyl-acyl carrier protein synthase in an active form
    J Shanklin
    Department of Biology, Brookhaven National Laboratory, Upton, New York, 11786, USA
    Protein Expr Purif 18:355-60. 2000
  7. ncbi request reprint Evidence linking the Pseudomonas oleovorans alkane omega-hydroxylase, an integral membrane diiron enzyme, and the fatty acid desaturase family
    John Shanklin
    Department of Biology, Brookhaven National Laboratory, Building 463, 50 Bell Ave, Upton, NY 11973, USA
    FEBS Lett 545:188-92. 2003
  8. pmc Desaturases: emerging models for understanding functional diversification of diiron-containing enzymes
    John Shanklin
    Department of Biology, Brookhaven National Laboratory, Upton, New York 11973, USA
    J Biol Chem 284:18559-63. 2009
  9. ncbi request reprint Engineering delta 9-16:0-acyl carrier protein (ACP) desaturase specificity based on combinatorial saturation mutagenesis and logical redesign of the castor delta 9-18:0-ACP desaturase
    E Whittle
    Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA
    J Biol Chem 276:21500-5. 2001
  10. ncbi request reprint Scanning transmission electron microscopy and small-angle scattering provide evidence that native Escherichia coli ClpP is a tetradecamer with an axial pore
    J M Flanagan
    Department of Biology, Brookhaven National Laboratory, Upton, New York 11973, USA
    Biochemistry 34:10910-7. 1995

Collaborators

  • Kimberly M Mayer
  • Peter H Buist
  • John A Browse
  • E B Cahoon
  • J Stubbe
  • Pradeep Kachroo
  • Edward Whittle
  • Ingo Heilmann
  • Jodie E Guy
  • Mark S Pidkowich
  • Ylva Lindqvist
  • Xiao Hong Yu
  • Martin Moche
  • Ying Lou
  • Behnaz Behrouzian
  • Edward J Whittle
  • Aardra Kachroo
  • Roman Davydov
  • John A Broadwater
  • E Whittle
  • Richa Rawat
  • Amy E Tremblay
  • Huu Tam Nguyen
  • Desigan Kumaran
  • Till Ischebeck
  • David Hildebrand
  • Ludmila Lapchyk
  • Isabel A Abreu
  • Stojan Smoukov
  • Brian M Hoffman
  • Satyam Subrahmanyam
  • Barbara King
  • Sergei Mekhedov
  • Thomas Girke
  • Alokesh Ghoshal
  • Christopher K Savile
  • Brian Dawson
  • J M Flanagan
  • M S Capel
  • J S Wall
  • D K Schneider

Detail Information

Publications24

  1. ncbi request reprint Desaturation and hydroxylation. Residues 148 and 324 of Arabidopsis FAD2, in addition to substrate chain length, exert a major influence in partitioning of catalytic specificity
    John A Broadwater
    Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA
    J Biol Chem 277:15613-20. 2002
    ....
  2. pmc Characterization and analysis of the cotton cyclopropane fatty acid synthase family and their contribution to cyclopropane fatty acid synthesis
    Xiao Hong Yu
    Department of Biochemistry and Cell Biology, Stony Brook University, NY, USA
    BMC Plant Biol 11:97. 2011
    ....
  3. pmc Identification of amino acid residues involved in substrate specificity of plant acyl-ACP thioesterases using a bioinformatics-guided approach
    Kimberly M Mayer
    Brookhaven National Laboratory, Department of Biology, Upton, NY 11973, USA
    BMC Plant Biol 7:1. 2007
    ..Variants were expressed in E. coli strain K27 that allows determination of enzyme activity by GCMS analysis of fatty acids released into the medium...
  4. pmc Linking enzyme sequence to function using Conserved Property Difference Locator to identify and annotate positions likely to control specific functionality
    Kimberly M Mayer
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA
    BMC Bioinformatics 6:284. 2005
    ....
  5. ncbi request reprint Exploring the possibilities presented by protein engineering
    J Shanklin
    Department of Biology, Brookhaven National Laboratory, Upton, NY 11786, USA
    Curr Opin Plant Biol 3:243-8. 2000
    ..These technologies have far-reaching consequences for the future design of crop plants and the storage compounds within them...
  6. ncbi request reprint Overexpression and purification of the Escherichia coli inner membrane enzyme acyl-acyl carrier protein synthase in an active form
    J Shanklin
    Department of Biology, Brookhaven National Laboratory, Upton, New York, 11786, USA
    Protein Expr Purif 18:355-60. 2000
    ....
  7. ncbi request reprint Evidence linking the Pseudomonas oleovorans alkane omega-hydroxylase, an integral membrane diiron enzyme, and the fatty acid desaturase family
    John Shanklin
    Department of Biology, Brookhaven National Laboratory, Building 463, 50 Bell Ave, Upton, NY 11973, USA
    FEBS Lett 545:188-92. 2003
    ....
  8. pmc Desaturases: emerging models for understanding functional diversification of diiron-containing enzymes
    John Shanklin
    Department of Biology, Brookhaven National Laboratory, Upton, New York 11973, USA
    J Biol Chem 284:18559-63. 2009
    ..We also summarize recent analysis of a castor mutant desaturase that provides valuable insights into the relationship of proposed substrate-binding modes with respect to a range of catalytic outcomes...
  9. ncbi request reprint Engineering delta 9-16:0-acyl carrier protein (ACP) desaturase specificity based on combinatorial saturation mutagenesis and logical redesign of the castor delta 9-18:0-ACP desaturase
    E Whittle
    Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA
    J Biol Chem 276:21500-5. 2001
    ....
  10. ncbi request reprint Scanning transmission electron microscopy and small-angle scattering provide evidence that native Escherichia coli ClpP is a tetradecamer with an axial pore
    J M Flanagan
    Department of Biology, Brookhaven National Laboratory, Upton, New York 11973, USA
    Biochemistry 34:10910-7. 1995
    ....
  11. pmc Evidence that the yeast desaturase Ole1p exists as a dimer in vivo
    Ying Lou
    Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA
    J Biol Chem 285:19384-90. 2010
    ....
  12. pmc Switching desaturase enzyme specificity by alternate subcellular targeting
    Ingo Heilmann
    Department of Biology, Brookhaven National Laboratory, Upton, NY 11973, USA
    Proc Natl Acad Sci U S A 101:10266-71. 2004
    ....
  13. pmc Revealing the catalytic potential of an acyl-ACP desaturase: tandem selective oxidation of saturated fatty acids
    Edward J Whittle
    Department of Biology, Brookhaven National Laboratory, 50 Bell Avenue, Upton, NY 11973, USA
    Proc Natl Acad Sci U S A 105:14738-43. 2008
    ..In summary, this study contributes detailed mechanistic insights into factors that govern the highly selective production of unusual fatty acids...
  14. ncbi request reprint Exploring the hydroxylation-dehydrogenation connection: novel catalytic activity of castor stearoyl-ACP Delta(9) desaturase
    Behnaz Behrouzian
    Department of Biology, Brookhaven National Laboratory, Upton, NY 11973, USA
    J Am Chem Soc 124:3277-83. 2002
    ..Hydroxylation and desaturation of this substrate share the same site of initial oxidative attack...
  15. ncbi request reprint A multifunctional acyl-acyl carrier protein desaturase from Hedera helix L. (English ivy) can synthesize 16- and 18-carbon monoene and diene products
    Edward Whittle
    Biology Department, Brookhaven National Laboratory, Upton, New York, 11973, USA
    J Biol Chem 280:28169-76. 2005
    ..The multifunctional properties of the ivy desaturase make it well suited for further dissection of the determinants of regiospecificity...
  16. pmc The stroma of higher plant plastids contain ClpP and ClpC, functional homologs of Escherichia coli ClpP and ClpA: an archetypal two-component ATP-dependent protease
    J Shanklin
    Department of Biology, Brookhaven National Laboratory, Upton, New York 11973, USA
    Plant Cell 7:1713-22. 1995
    ..This demonstrates that ClpC is a functional homolog of E. coli ClpA and not of ClpB or ClpX. These data represent the only in vitro demonstration of the activity of a specific ATP-dependent chloroplast protease reported to date...
  17. ncbi request reprint A structural model of the plant acyl-acyl carrier protein thioesterase FatB comprises two helix/4-stranded sheet domains, the N-terminal domain containing residues that affect specificity and the C-terminal domain containing catalytic residues
    Kimberly M Mayer
    Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA
    J Biol Chem 280:3621-7. 2005
    ..Together these data corroborate the structural model and show that the hot dog fold is common to enzymes from both prokaryotes and eukaryotes and that this fold supports at least three different catalytic mechanisms...
  18. pmc Modulating seed beta-ketoacyl-acyl carrier protein synthase II level converts the composition of a temperate seed oil to that of a palm-like tropical oil
    Mark S Pidkowich
    Department of Biology, Brookhaven National Laboratory, Upton, NY 11973, USA
    Proc Natl Acad Sci U S A 104:4742-7. 2007
    ..These results resolve the role of KASII in seed metabolism and demonstrate that modulation of Arabidopsis KASII levels is sufficient to convert its temperate oilseed composition to that of a palm-like tropical oil...
  19. pmc Identification of the Arabidopsis palmitoyl-monogalactosyldiacylglycerol delta7-desaturase gene FAD5, and effects of plastidial retargeting of Arabidopsis desaturases on the fad5 mutant phenotype
    Ingo Heilmann
    Department of Biology, Brookhaven National Laboratory, Upton, New York 11973, USA
    Plant Physiol 136:4237-45. 2004
    ..Tight correlation between leaf 16:3Delta(7,10,13) levels and chlorophyll content suggests a role for plastidial fatty acid desaturases in thylakoid formation...
  20. ncbi request reprint The Arabidopsis stearoyl-acyl carrier protein-desaturase family and the contribution of leaf isoforms to oleic acid synthesis
    Aardra Kachroo
    Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA
    Plant Mol Biol 63:257-71. 2007
    ..Enzyme activities of the desaturase isoforms in a 5-fold excess of 18:1-ACP show product inhibition of up to 73%. Together these data indicate that 18:1 levels are regulated at both transcriptional and post-translational levels...
  21. pmc A single mutation in the castor Delta9-18:0-desaturase changes reaction partitioning from desaturation to oxidase chemistry
    Jodie E Guy
    Department of Medical Biochemistry and Biophysics, Division of Molecular Structural Biology, Karolinska Institutet, Tomtebodavägen 6, S 171 77 Stockholm, Sweden
    Proc Natl Acad Sci U S A 103:17220-4. 2006
    ..That a single amino acid substitution can switch reactivity from desaturation to oxidation provides experimental support for the hypothesis that the desaturase evolved from an ancestral oxidase enzyme...
  22. ncbi request reprint The crystal structure of the ivy Delta4-16:0-ACP desaturase reveals structural details of the oxidized active site and potential determinants of regioselectivity
    Jodie E Guy
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77 Stockholm, Sweden
    J Biol Chem 282:19863-71. 2007
    ....
  23. ncbi request reprint Azide and acetate complexes plus two iron-depleted crystal structures of the di-iron enzyme delta9 stearoyl-acyl carrier protein desaturase. Implications for oxygen activation and catalytic intermediates
    Martin Moche
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm S 171 77, Sweden
    J Biol Chem 278:25072-80. 2003
    ....
  24. ncbi request reprint Effect of substrate on the diiron(III) site in stearoyl acyl carrier protein delta 9-desaturase as disclosed by cryoreduction electron paramagnetic resonance/electron nuclear double resonance spectroscopy
    Roman Davydov
    Department of Chemistry, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208 3113, USA
    Biochemistry 44:1309-15. 2005
    ..These structural alterations may provide DS with an additional mechanism for tuning the redox potential of the diferric site. The mixed-valence states of DS are unstable at temperatures above 230 K...