Allen M Orville

Summary

Affiliation: Brookhaven National Laboratory
Country: USA

Publications

  1. pmc Correlated single-crystal electronic absorption spectroscopy and X-ray crystallography at NSLS beamline X26-C
    Allen M Orville
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973 5000, USA
    J Synchrotron Radiat 18:358-66. 2011
  2. pmc Crystallographic, spectroscopic, and computational analysis of a flavin C4a-oxygen adduct in choline oxidase
    Allen M Orville
    Biology Department, Brookhaven National Laboratory, Upton, New York 11973 5000, School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia 30332 0400, USA
    Biochemistry 48:720-8. 2009
  3. ncbi request reprint The crystal structure of NAD(P)H oxidase from Lactobacillus sanfranciscensis: insights into the conversion of O2 into two water molecules by the flavoenzyme
    George T Lountos
    School of Chemistry and Biochemistry, Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia 30332, USA
    Biochemistry 45:9648-59. 2006
  4. pmc Crystal structures of nitroalkane oxidase: insights into the reaction mechanism from a covalent complex of the flavoenzyme trapped during turnover
    Akanksha Nagpal
    School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia 30332 0400, USA
    Biochemistry 45:1138-50. 2006
  5. pmc Crystal structures of intermediates in the nitroalkane oxidase reaction
    Annie Heroux
    Department of Biology, Brookhaven National Laboratory, Upton, New York 11973, USA
    Biochemistry 48:3407-16. 2009
  6. ncbi request reprint Role of Glu312 in binding and positioning of the substrate for the hydride transfer reaction in choline oxidase
    Osbourne Quaye
    Department of Chemistry, Georgia State University, Atlanta, Georgia 30302 4098, USA
    Biochemistry 47:243-56. 2008
  7. ncbi request reprint Crystal structures and functional studies of T4moD, the toluene 4-monooxygenase catalytic effector protein
    George T Lountos
    School of Chemistry and Biochemistry, Parker H Petit Institute of Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia 30332 0400, USA
    Biochemistry 44:7131-42. 2005
  8. doi request reprint Structural and kinetic studies on the Ser101Ala variant of choline oxidase: catalysis by compromise
    Steffan Finnegan
    Department of Chemistry, Georgia State University, Atlanta, GA 30302 4098, USA
    Arch Biochem Biophys 501:207-13. 2010
  9. pmc Acoustic methods for high-throughput protein crystal mounting at next-generation macromolecular crystallographic beamlines
    Christian G Roessler
    Photon Sciences Directorate, Brookhaven National Laboratory, Upton, NY 11973, USA
    J Synchrotron Radiat 20:805-8. 2013
  10. ncbi request reprint Crystallization and preliminary analysis of xenobiotic reductase B from Pseudomonas fluorescens I-C
    Allen M Orville
    School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332 0400, USA
    Acta Crystallogr D Biol Crystallogr 60:1289-91. 2004

Collaborators

Detail Information

Publications16

  1. pmc Correlated single-crystal electronic absorption spectroscopy and X-ray crystallography at NSLS beamline X26-C
    Allen M Orville
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973 5000, USA
    J Synchrotron Radiat 18:358-66. 2011
    ..Data are now routinely generated almost simultaneously from three complementary types of experiments from the same sample. The beamline is available now to the NSLS general user population...
  2. pmc Crystallographic, spectroscopic, and computational analysis of a flavin C4a-oxygen adduct in choline oxidase
    Allen M Orville
    Biology Department, Brookhaven National Laboratory, Upton, New York 11973 5000, School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia 30332 0400, USA
    Biochemistry 48:720-8. 2009
    ..We propose that the enzyme-bound FAD is reduced in the X-ray beam. The aerobic crystals then form either a C4a-OH or C4a-OO(H) adduct, but an insufficient proton inventory prevents their decay at cryogenic temperatures...
  3. ncbi request reprint The crystal structure of NAD(P)H oxidase from Lactobacillus sanfranciscensis: insights into the conversion of O2 into two water molecules by the flavoenzyme
    George T Lountos
    School of Chemistry and Biochemistry, Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia 30332, USA
    Biochemistry 45:9648-59. 2006
    ..HPLC analysis of ligands released from denatured L.lac-Nox2 indicates that it does not bind ADP, which correlates with the specificity of the enzyme for oxidation of NADH...
  4. pmc Crystal structures of nitroalkane oxidase: insights into the reaction mechanism from a covalent complex of the flavoenzyme trapped during turnover
    Akanksha Nagpal
    School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia 30332 0400, USA
    Biochemistry 45:1138-50. 2006
    ..These likely differentiate NAO from the homologues and, consequently, are proposed to result in the unique reaction mechanism of NAO...
  5. pmc Crystal structures of intermediates in the nitroalkane oxidase reaction
    Annie Heroux
    Department of Biology, Brookhaven National Laboratory, Upton, New York 11973, USA
    Biochemistry 48:3407-16. 2009
    ..Consequently, these results provide important structural descriptions of several steps along the nitroalkane oxidase reaction cycle...
  6. ncbi request reprint Role of Glu312 in binding and positioning of the substrate for the hydride transfer reaction in choline oxidase
    Osbourne Quaye
    Department of Chemistry, Georgia State University, Atlanta, Georgia 30302 4098, USA
    Biochemistry 47:243-56. 2008
    ..Altogether, the crystallographic and mechanistic data suggest that Glu312 is important for binding and positioning of the substrate in the active site of choline oxidase...
  7. ncbi request reprint Crystal structures and functional studies of T4moD, the toluene 4-monooxygenase catalytic effector protein
    George T Lountos
    School of Chemistry and Biochemistry, Parker H Petit Institute of Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia 30332 0400, USA
    Biochemistry 44:7131-42. 2005
    ..The relative insensitivity to changes in the N-terminal region distinguishes T4moD from the MmoB homologues, which each require the approximately 33 residue N-terminal region for catalytic activity...
  8. doi request reprint Structural and kinetic studies on the Ser101Ala variant of choline oxidase: catalysis by compromise
    Steffan Finnegan
    Department of Chemistry, Georgia State University, Atlanta, GA 30302 4098, USA
    Arch Biochem Biophys 501:207-13. 2010
    ....
  9. pmc Acoustic methods for high-throughput protein crystal mounting at next-generation macromolecular crystallographic beamlines
    Christian G Roessler
    Photon Sciences Directorate, Brookhaven National Laboratory, Upton, NY 11973, USA
    J Synchrotron Radiat 20:805-8. 2013
    ..Future developments and implementation will focus on achieving ejection and translation of single droplets at a rate of over one hundred per second...
  10. ncbi request reprint Crystallization and preliminary analysis of xenobiotic reductase B from Pseudomonas fluorescens I-C
    Allen M Orville
    School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332 0400, USA
    Acta Crystallogr D Biol Crystallogr 60:1289-91. 2004
    ..6 A. The asymmetric unit is likely to contain at least two XenB molecules (V(M) = 3.1 A(3) Da(-1), 60% solvent) and a molecular-replacement solution has been determined in order to solve the structure...
  11. ncbi request reprint Crystallization and preliminary analysis of xenobiotic reductase A and ligand complexes from Pseudomonas putida II-B
    Allen M Orville
    School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332 0400, USA
    Acta Crystallogr D Biol Crystallogr 60:957-61. 2004
    ..Thus, the local symmetry produces approximate systematic absences along the (00l) principal axis and complicates the space-group determination...
  12. pmc Single-crystal Raman spectroscopy and X-ray crystallography at beamline X26-C of the NSLS
    Deborah Stoner-Ma
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA
    J Synchrotron Radiat 18:37-40. 2011
    ....
  13. ncbi request reprint Crystallization and preliminary analysis of a water-forming NADH oxidase from Lactobacillus sanfranciscensis
    George T Lountos
    School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332 0400, USA
    Acta Crystallogr D Biol Crystallogr 60:2044-7. 2004
    ..3 A(3) Da(-1), 45.5% solvent content), which has been confirmed by the molecular-replacement solution using a search molecule derived from NADH peroxidase (PDB code 1f8w)...
  14. pmc Crystallization and preliminary analysis of active nitroalkane oxidase in three crystal forms
    Akanksha Nagpal
    School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta 30332 0400, USA
    Acta Crystallogr D Biol Crystallogr 60:1456-60. 2004
    ..A three-wavelength MAD data set was collected from selenomethionine-enriched NAO (SeMet-NAO) in crystal form 3 and will be used for phasing...
  15. pmc Acoustically mounted microcrystals yield high-resolution X-ray structures
    Alexei S Soares
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973 5000, USA
    Biochemistry 50:4399-401. 2011
    ..X-ray diffraction data sets merged from several micrometer-sized crystals are used to determine 1.8 Ǻ resolution crystal structures...
  16. ncbi request reprint Crystallization and preliminary analysis of native and N-terminal truncated isoforms of toluene-4-monooxygenase catalytic effector protein
    Allen M Orville
    School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332 0400, USA
    Acta Crystallogr D Biol Crystallogr 59:572-5. 2003
    ..These crystallographic results are the first available for the evolutionarily related but functionally diversified catalytic effector proteins from the multicomponent diiron monooxygenase family...