Huilin Li

Summary

Affiliation: Brookhaven National Laboratory
Country: USA

Publications

  1. pmc Structure of the Haemophilus influenzae HMW1B translocator protein: evidence for a twin pore
    Huilin Li
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA
    J Bacteriol 189:7497-502. 2007
  2. pmc Dimeric organization of the yeast oligosaccharyl transferase complex
    Manasi Chavan
    Department of Biochemistry and Cell Biology and Institute for Cell and Developmental Biology, Stony Brook University, Stony Brook, NY 11794, USA
    Proc Natl Acad Sci U S A 103:8947-52. 2006
  3. pmc Binding-induced folding of prokaryotic ubiquitin-like protein on the Mycobacterium proteasomal ATPase targets substrates for degradation
    Tao Wang
    Biology Department, Brookhaven National Laboratory, Upton, New York, USA
    Nat Struct Mol Biol 17:1352-7. 2010
  4. pmc The origin recognition complex: a biochemical and structural view
    Huilin Li
    Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY, 11794, USA
    Subcell Biochem 62:37-58. 2012
  5. pmc Use of a combined cryo-EM and X-ray crystallography approach to reveal molecular details of bacterial pilus assembly by the chaperone/usher pathway
    Huilin Li
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA
    Curr Opin Microbiol 12:326-32. 2009
  6. pmc Toward structural elucidation of the gamma-secretase complex
    Huilin Li
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973 5000, USA
    Structure 17:326-34. 2009
  7. pmc Oligosaccharyltransferase directly binds to ribosome at a location near the translocon-binding site
    Yoichiro Harada
    Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY 11794, USA
    Proc Natl Acad Sci U S A 106:6945-9. 2009
  8. pmc Structural insights on the Mycobacterium tuberculosis proteasomal ATPase Mpa
    Tao Wang
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973 5000, USA
    Structure 17:1377-85. 2009
  9. pmc Cryo-EM structure of a helicase loading intermediate containing ORC-Cdc6-Cdt1-MCM2-7 bound to DNA
    Jingchuan Sun
    Biosciences Department, Brookhaven National Laboratory, Upton, New York, USA
    Nat Struct Mol Biol 20:944-51. 2013
  10. pmc Structural studies and the assembly of the heptameric post-translational translocon complex
    Yoichiro Harada
    Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, New York 11794, USA
    J Biol Chem 286:2956-65. 2011

Collaborators

Detail Information

Publications38

  1. pmc Structure of the Haemophilus influenzae HMW1B translocator protein: evidence for a twin pore
    Huilin Li
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA
    J Bacteriol 189:7497-502. 2007
    ..These observations suggest that HMW1B undergoes a large conformational change during translocation of the 125-kDa HMW1 adhesin...
  2. pmc Dimeric organization of the yeast oligosaccharyl transferase complex
    Manasi Chavan
    Department of Biochemistry and Cell Biology and Institute for Cell and Developmental Biology, Stony Brook University, Stony Brook, NY 11794, USA
    Proc Natl Acad Sci U S A 103:8947-52. 2006
    ..We suggest that the dimeric structure of OT might be required for effective association with the translocon dimer and for its allosteric regulation during cotranslational glycosylation...
  3. pmc Binding-induced folding of prokaryotic ubiquitin-like protein on the Mycobacterium proteasomal ATPase targets substrates for degradation
    Tao Wang
    Biology Department, Brookhaven National Laboratory, Upton, New York, USA
    Nat Struct Mol Biol 17:1352-7. 2010
    ..This key difference between the prokaryotic and eukaryotic systems could be exploited for the development of a small molecule-based treatment for tuberculosis...
  4. pmc The origin recognition complex: a biochemical and structural view
    Huilin Li
    Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY, 11794, USA
    Subcell Biochem 62:37-58. 2012
    ....
  5. pmc Use of a combined cryo-EM and X-ray crystallography approach to reveal molecular details of bacterial pilus assembly by the chaperone/usher pathway
    Huilin Li
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA
    Curr Opin Microbiol 12:326-32. 2009
    ..Such a combined approach holds promise for further elucidating remaining questions regarding the multi-step and highly dynamic pilus assembly process, as well as for studying other protein secretion and organelle biogenesis systems...
  6. pmc Toward structural elucidation of the gamma-secretase complex
    Huilin Li
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973 5000, USA
    Structure 17:326-34. 2009
    ..Here, we review recent progress on biochemical and biophysical probing of the structure of the four-component complex and discuss obstacles and potential pathways toward elucidating its detailed structure...
  7. pmc Oligosaccharyltransferase directly binds to ribosome at a location near the translocon-binding site
    Yoichiro Harada
    Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY 11794, USA
    Proc Natl Acad Sci U S A 106:6945-9. 2009
    ..Based on existing data and our findings, we propose that cotranslational translocation and N-glycosylation of nascent polypeptides are mediated by a ternary supramolecular complex consisting of OT, the Sec61 complex, and ribosomes...
  8. pmc Structural insights on the Mycobacterium tuberculosis proteasomal ATPase Mpa
    Tao Wang
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973 5000, USA
    Structure 17:1377-85. 2009
    ..These studies provide insights into how a bacterial proteasomal ATPase interacts with and facilitates protein degradation by the proteasome...
  9. pmc Cryo-EM structure of a helicase loading intermediate containing ORC-Cdc6-Cdt1-MCM2-7 bound to DNA
    Jingchuan Sun
    Biosciences Department, Brookhaven National Laboratory, Upton, New York, USA
    Nat Struct Mol Biol 20:944-51. 2013
    ..The resulting ORC-Cdc6 helicase loader shows a notable structural similarity to the replication factor C clamp loader, suggesting a conserved mechanism of action. ..
  10. pmc Structural studies and the assembly of the heptameric post-translational translocon complex
    Yoichiro Harada
    Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, New York 11794, USA
    J Biol Chem 286:2956-65. 2011
    ..Mutations of conserved, positively charged amino acid residues in the loop caused decreased formation of the post-translocon. These studies provide the first architectural description of the yeast post-translocon...
  11. doi request reprint Structure of the oligosaccharyl transferase complex at 12 A resolution
    Hua Li
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973 5000, USA
    Structure 16:432-40. 2008
    ..A prominent groove was observed between these subunits, and we propose that the nascent polypeptide from the translocon threads through this groove while being scanned by the Ost1p subunit for the presence of the glycosylation sequon...
  12. ncbi request reprint Structure of the Mycobacterium tuberculosis proteasome and mechanism of inhibition by a peptidyl boronate
    Guiqing Hu
    Biology Department, Brookhaven National Laboratory, 50 Bell Avenue, Upton, NY 11973, USA
    Mol Microbiol 59:1417-28. 2006
    ..The structure improves prospects for designing Mtb-specific proteasomal inhibitors as a novel approach to chemotherapy of tuberculosis...
  13. pmc Cdc6-induced conformational changes in ORC bound to origin DNA revealed by cryo-electron microscopy
    Jingchuan Sun
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA
    Structure 20:534-44. 2012
    ..Thus, ORC bends and wraps the DNA. This model is consistent with the observation that binding of a single Cdc6 extends the ORC footprint on origin DNA from both ends...
  14. pmc Structural basis for the assembly and gate closure mechanisms of the Mycobacterium tuberculosis 20S proteasome
    Dongyang Li
    Department of Biology, Brookhaven National Laboratory, Upton, NY, USA
    EMBO J 29:2037-47. 2010
    ..Our work provides the structural bases for assembly and gating mechanisms of the Mtb proteasome...
  15. pmc The architecture of the DNA replication origin recognition complex in Saccharomyces cerevisiae
    Zhiqiang Chen
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA
    Proc Natl Acad Sci U S A 105:10326-31. 2008
    ..The studies provide a basis for understanding the overall structure of the pre-RC...
  16. ncbi request reprint The outer membrane usher forms a twin-pore secretion complex
    Huilin Li
    Biology Department, Brookhaven National Laboratory, 50 Bell Ave, Upton, NY 11973, USA
    J Mol Biol 344:1397-407. 2004
    ..The overall molecular size (11 nm), pore size (2 nm), and twin-pore configuration of PapC resemble that of the Tom40 complex, a mitochondrial outer membrane protein translocase...
  17. pmc Inhibitors selective for mycobacterial versus human proteasomes
    Gang Lin
    Department of Microbiology and Immunology, Weill Cornell Medical College, New York, New York 10065, USA
    Nature 461:621-6. 2009
    ..This may account for the ability of oxathiazol-2-one compounds to inhibit the mycobacterial proteasome potently and irreversibly while largely sparing the human homologue...
  18. ncbi request reprint Mycobacterium tuberculosis prcBA genes encode a gated proteasome with broad oligopeptide specificity
    Gang Lin
    Department of Microbiology and Immunology, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Mol Microbiol 59:1405-16. 2006
    ..Thus, Mtb encodes a broadly active, gated proteasome that may work in concert with an endogenous activator...
  19. pmc Quaternary organization of a phytochrome dimer as revealed by cryoelectron microscopy
    Hua Li
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973 5000, USA
    Proc Natl Acad Sci U S A 107:10872-7. 2010
    ....
  20. pmc "Depupylation" of prokaryotic ubiquitin-like protein from mycobacterial proteasome substrates
    Kristin E Burns
    Department of Microbiology, New York University School of Medicine, New York, NY 10016, USA
    Mol Cell 39:821-7. 2010
    ..Here, we show that Mycobacteria have a "depupylase" activity provided by Dop. The discovery of a depupylase strengthens the parallels between the Pup- and Ub-tagging systems of prokaryotes and eukaryotes, respectively...
  21. ncbi request reprint Protein secretion in the absence of ATP: the autotransporter, two-partner secretion and chaperone/usher pathways of gram-negative bacteria (review)
    David G Thanassi
    Center for Infectious Diseases, Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York 11794 5120, USA
    Mol Membr Biol 22:63-72. 2005
    ..This review will present overviews of these 'self-sufficient' pathways, focusing on recent advances and secretion mechanisms. Similarities among the pathways and with other protein translocation mechanisms will be highlighted...
  22. ncbi request reprint Characterization of a Mycobacterium tuberculosis proteasomal ATPase homologue
    K Heran Darwin
    Department of Microbiology and Immunology, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
    Mol Microbiol 55:561-71. 2005
    ..The corresponding strain was attenuated in mice. Thus, Mpa is an ATPase whose enzymatic activity is necessary but not sufficient to protect against reactive nitrogen intermediates...
  23. pmc Electron microscopic structure of purified, active gamma-secretase reveals an aqueous intramembrane chamber and two pores
    Vlado K Lazarov
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA
    Proc Natl Acad Sci U S A 103:6889-94. 2006
    ..Our reconstructed 3D map provides a physical basis for hydrolysis of transmembrane substrates within a lipid bilayer and release of the products into distinct subcellular compartments...
  24. pmc Inhibitors of amyloid toxicity based on beta-sheet packing of Abeta40 and Abeta42
    Takeshi Sato
    Department of Biochemistry and Cell Biology, Center for Structural Biology, Stony Brook University, Stony Brook, New York 11794 5215, USA
    Biochemistry 45:5503-16. 2006
    ..Importantly, the designed peptide inhibitors significantly reduce the toxicity induced by Abeta42 on cultured rat cortical neurons...
  25. pmc Crystal structure of the coat protein of the flexible filamentous papaya mosaic virus
    Shaoqing Yang
    Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA
    J Mol Biol 422:263-73. 2012
    ..The structure of PapMV will be useful for rational design and engineering of the PapMV nanoparticles into innovative vaccines...
  26. ncbi request reprint Oligomeric state of the Escherichia coli metal transporter YiiP
    Yinan Wei
    Department of Biology, Brookhaven National Laboratory, Upton, New York 11973, USA
    J Biol Chem 279:39251-9. 2004
    ..These data provide direct structural evidence for a dimeric association of YiiP both in detergent-lipid micelles and in the reconstituted lipid bilayer. The functional relevance of the dimeric association in YiiP is discussed...
  27. pmc Quantitation and composition of cutaneous microbiota in diabetic and nondiabetic men
    Henry Redel
    Department of Medicine, New York University School of Medicine, New York, NY 10010, USA
    J Infect Dis 207:1105-14. 2013
    ..Diabetic foot infections are a leading cause of lower extremity amputations. Our study examines the microbiota of diabetic skin prior to ulcer development or infection...
  28. pmc Crystallographic study of FABP5 as an intracellular endocannabinoid transporter
    Benoît Sanson
    Biosciences Department, Brookhaven National Laboratory, Upton, NY 11973 5000, USA
    Acta Crystallogr D Biol Crystallogr 70:290-8. 2014
    ..This work advances our understanding of FABP5-endocannabinoid interactions and may be useful for future efforts in the development of small-molecule inhibitors to raise endocannabinoid levels. ..
  29. doi request reprint The Pup-Proteasome System of Mycobacterium tuberculosis
    Marie I Samanovic
    Department of Microbiology, New York University School of Medicine, 550 First Avenue, MSB 236, New York, NY, 10016, USA
    Subcell Biochem 66:267-95. 2013
    ..Furthermore, the understanding of proteasome function in Mtb has helped reveal new insight into how the host battles infections...
  30. doi request reprint How to operate a cryo-electron microscope
    Jingchuan Sun
    Biology Department, Brookhaven National Laboratory, Upton, New York, USA
    Methods Enzymol 481:231-49. 2010
    ..Despite recent advances in instrumentation, the microscopist's patience and attention to detail may still be the key to acquiring a high quality cryo-electron micrograph...
  31. ncbi request reprint Huntingtin spheroids and protofibrils as precursors in polyglutamine fibrilization
    Michelle A Poirier
    Division of Neurobiology, Department of Psychiatry, Johns Hopkins University School of Medicine, 615 Ross Research Building, 720 Rutland Avenue, Baltimore, MD 21205, USA
    J Biol Chem 277:41032-7. 2002
    ....
  32. ncbi request reprint Microtubule structure at 8 A resolution
    Huilin Li
    Life Sciences Division, Lawrence Berkeley National Laboratory, CA 94720, USA
    Structure 10:1317-28. 2002
    ....
  33. ncbi request reprint The binding mode of epothilone A on alpha,beta-tubulin by electron crystallography
    James H Nettles
    Molecular and Systems Pharmacology, Emory University, Atlanta, GA 30322, USA
    Science 305:866-9. 2004
    ..Comparison with Taxol shows that the longstanding expectation of a common pharmacophore is not met, because each ligand exploits the tubulin-binding pocket in a unique and independent manner...
  34. pmc Evidence for conservation of architecture and physical properties of Omp85-like proteins throughout evolution
    Neeraj K Surana
    The Edward Mallinckrodt Department of Pediatrics and Department of Molecular Microbiology, Washington University School of Medicine, Campus Box 8208, 660 South Euclid Avenue, St Louis, MO 63110, USA
    Proc Natl Acad Sci U S A 101:14497-502. 2004
    ....
  35. ncbi request reprint KChIP2 modulates the cell surface expression of Kv 1.5-encoded K(+) channels
    Huilin Li
    Department of Molecular Biology and Pharmacology, Washington University Medical School, 660 South Euclid Avenue, Saint Louis, MO 63110, USA
    J Mol Cell Cardiol 39:121-32. 2005
    ..5-encoded) I(K,slow1) channels as well, perhaps, as other Kv1.5-encoded K(+) currents, including I(Kur) (I(K,ultrarapid)), in human atria...
  36. ncbi request reprint Structure of the CED-4-CED-9 complex provides insights into programmed cell death in Caenorhabditis elegans
    Nieng Yan
    Department of Molecular Biology, Princeton University, Lewis Thomas Laboratory, Washington Road, Princeton, New Jersey 08544, USA
    Nature 437:831-7. 2005
    ..The released CED-4 dimer further dimerizes to form a tetramer, which facilitates the autoactivation of CED-3. Together, our studies provide important insights into the regulation of cell death activation in C. elegans...
  37. ncbi request reprint Role of heteromultimers in the generation of myocardial transient outward K+ currents
    Weinong Guo
    Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St Louis, MO 63110, USA
    Circ Res 90:586-93. 2002
    ..2/Kv4.3 alpha subunits and KChIP2. The results here also suggest that Kv4.2 is the primary determinant of the regional heterogeneity in I(to,f) expression in adult mouse ventricle...

Research Grants10

  1. The Structural Basis of Eukaryotic Replication Origin Licensing by Cryo-EM
    Huilin Li; Fiscal Year: 2006
    ..These detailed structural studies will provide a long-awaited molecular mechanism for the origin licensing stage of the intricate replication initiation process. ..
  2. The Structural Basis of Eukaryotic Replication Origin Licensing by Cryo-EM
    Huilin Li; Fiscal Year: 2010
    ..These detailed structural studies will provide a long-awaited molecular mechanism for the origin licensing stage of the intricate replication initiation process. ..
  3. Structural studies of the M. tuberculosis proteasome and proteasomal ATPase
    Huilin Li; Fiscal Year: 2007
    ....
  4. Structural studies of the M. tuberculosis proteasome and proteasomal ATPase
    Huilin Li; Fiscal Year: 2010
    ....
  5. The Structural Basis of Eukaryotic Replication Origin Licensing by Cryo-EM
    Huilin Li; Fiscal Year: 2009
    ..These detailed structural studies will provide a long-awaited molecular mechanism for the origin licensing stage of the intricate replication initiation process. ..
  6. Structural studies of the M. tuberculosis proteasome and proteasomal ATPase
    Huilin Li; Fiscal Year: 2009
    ....
  7. The Structural Basis of Eukaryotic Replication Origin Licensing by Cryo-EM
    Huilin Li; Fiscal Year: 2007
    ..These detailed structural studies will provide a long-awaited molecular mechanism for the origin licensing stage of the intricate replication initiation process. ..
  8. The Structural Basis of Eukaryotic Replication Origin Licensing by Cryo-EM
    Huilin Li; Fiscal Year: 2009
    ..These detailed structural studies will provide a long-awaited molecular mechanism for the origin licensing stage of the intricate replication initiation process. ..