Research Topics
Species | Andrew N GiffordSummaryAffiliation: Brookhaven National Laboratory Country: USA Publications
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Publications
123I-labeled AM251: a radioiodinated ligand which binds in vivo to mouse brain cannabinoid CB1 receptorsS J Gatley
Medical Department, Brookhaven National Laboratory, Upton, NY 11973, USA
Eur J Pharmacol 307:331-8. 1996..08). In in vitro binding assays with mouse hippocampal membranes, tetrahydrocannabinol inhibited binding of [123I]AM251 with an IC50 value of about 700 nM, compared with about 0.2 nM for SR141716A...
Polymer-supported organotin reagent for prosthetic group labeling of biological macromolecules with radioiodineAndrew N Gifford
Medical Department, Brookhaven National Laboratory, Upton, New York 11973, USA
Bioconjug Chem 22:406-12. 2011..This solid-phase approach is potentially adaptable to kit-formulation for performing conjugate radiolabeling of biological macromolecules...- In vivo imaging of the brain cannabinoid receptorAndrew N Gifford
Medical Department, Brookhaven National Laboratory, Upton, NY 11973, USA
Chem Phys Lipids 121:65-72. 2002..Although rodent studies have indicated that in vivo imaging of CB1 receptors is feasible, at the present time this receptor has still to be successful imaged in a human PET study... - Effect of amphetamine-induced dopamine release on radiotracer binding to D1 and D2 receptors in rat brain striatal slicesA N Gifford
Medical Department, Brookhaven National Laboratory, Upton, NY 11973, USA
Naunyn Schmiedebergs Arch Pharmacol 362:413-8. 2000.... 
Cannabinoid receptor-mediated inhibition of acetylcholine release from hippocampal and cortical synaptosomesA N Gifford
Medical Department, Brookhaven National Laboratory, Upton, New York, NY 11973, USA
Br J Pharmacol 131:645-50. 2000..The SR 141716A-induced enhancement of ACh release can similarly be observed in hippocampal synaptosomes and is probably due to an inverse agonist action at constitutively active receptors...- Potentiation of the action of anandamide on hippocampal slices by the fatty acid amide hydrolase inhibitor, palmitylsulphonyl fluoride (AM 374)A N Gifford
Medical Department, Brookhaven National Laboratory, Upton, NY 11973, USA
Eur J Pharmacol 383:9-14. 1999..These results indicate that AM 374 potently inhibits endogenous amidase activity and thus facilitates access of exogenous anandamide to cannabinoid receptors in the hippocampal tissue... - Large receptor reserve for cannabinoid actions in the central nervous systemA N Gifford
Medical Department, Brookhaven National Laboratory, Upton, New York 11973, USA
J Pharmacol Exp Ther 288:478-83. 1999..p.), completely abolished specific [131I]AM 281 binding. These experiments suggest that behavioral effects of cannabinoids, like neurochemical effects, are produced at very low receptor occupancy... - Biodistribution of radiolabeled ethanol in rodentsAndrew N Gifford
Medical Department, Brookhaven National Laboratory, Upton, NNew York 11973, USA
Drug Metab Dispos 36:1853-8. 2008..It is thus tempting to speculate that the well documented high incidence of pancreatic disease observed in human chronic alcoholism may be related to a propensity of this organ to accumulate ethanol and/or reactive ethanol metabolites... - Evaluation of the in vivo receptor occupancy for the behavioral effects of cannabinoids using a radiolabeled cannabinoid receptor agonist, R-[125/131I]AM2233Jasbeer Dhawan
Medical Department, Brookhaven National Laboratory, Upton, New York 11973, USA
Synapse 60:93-101. 2006..These observations suggest that the behavioral effects of CB1 receptor agonists are manifested with a relatively small fraction of the agonist-preferring affinity state of the receptor occupied... - Ex vivo imaging of fatty acid amide hydrolase activity and its inhibition in the mouse brainSherrye T Glaser
Center for Translational Neuroimaging, Medical Department, Bldg 490, 30 Bell Ave, Brookhaven National Laboratory, Upton, NY 11973, USA
J Pharmacol Exp Ther 316:1088-97. 2006..This imaging method determines regionally specific FAAH inhibition and can elucidate the in vivo effects of pharmacological agents targeting anandamide inactivation... - Concentration-dependent conditioned place preference to inhaled toluene vapors in ratsDianne E Lee
Chemistry Department, Brookhaven National Laboratory, Upton, NY 11973, USA
Drug Alcohol Depend 85:87-90. 2006.... - Effect of SA4503 on the electrically evoked release of (3)H-acetylcholine from striatal and hippocampal rat brain slicesBryan Horan
Medical Department, Brookhaven National Laboratories, Upton, New York 11973, USA
Synapse 46:1-3. 2002.... - Study of brain uptake and biodistribution of [11C]toluene in non-human primates and miceM R Gerasimov
Chemistry Department, Brookhaven National Laboratory, Upton, New York 11973, USA
Life Sci 70:2811-28. 2002..Taken together, our data provides insight into pharmacokinetic features possibly associated with the abuse liability of toluene... - Binding of the non-classical cannabinoid CP 55,940, and the diarylpyrazole AM251 to rodent brain cannabinoid receptorsS J Gatley
Medical Department, Brookhaven National Laboratory, Upton, NY 11973, USA
Life Sci 61:PL 191-7. 1997..However, the binding site domains which interact with AM251 and CP 55,940 may not be identical, since IC50 values for cannabinoid receptor ligands depended on whether [123I]AM251 or [3H]CP 55,940 was used as radioligand... - In vitro and ex vivo autoradiographic studies of nicotinic acetylcholine receptors using [18F]fluoronochloroepibatidine in rodent and human brainS J Gatley
Medical Department, Brookhaven National Laboratory, Upton, NY 11973, USA
Nucl Med Biol 25:449-54. 1998..However, similar Kd values close to 50 pM were calculated for all regions. These studies support the suitability of [18F]NFEP as a radioligand for PET studies of nAcChR in the living human brain... - Dopamine-transporter occupancy after intravenous doses of cocaine and methylphenidate in mice and humansS J Gatley
Medical Department, Brookhaven National Laboratory, Upton, NY 11973, USA
Psychopharmacology (Berl) 146:93-100. 1999..The present experiments were conducted to examine dose-occupancy and dose-effect relationships in mice for cocaine and also for methylphenidate, a dopamine uptake blocker used in pediatric psychiatry... - Sensitivity of binding of high-affinity dopamine receptor radioligands to increased synaptic dopamineS J Gatley
Medical Department, Brookhaven National Laboratory, Upton, New York 11973, USA
Synapse 38:483-8. 2000..We suggest that the magnitude of the competition is reduced by failure of the receptor binding of high-affinity radioligands to rapidly attain equilibrium... - Effects of endogenous neurotransmitters on the in vivo binding of dopamine and 5-HT radiotracers in miceO V Rice
Medical Department, Brookhaven National Laboratory, Upton, NY 11973, USA
Neuropsychopharmacology 25:679-89. 2001..3H]N-methylspiperone binding to 5-HT(2A) receptors and [3H]WAY 100635 binding to 5-HT(1A) receptors appeared insensitive to changes in neurotransmitter levels... - "Nonhedonic" food motivation in humans involves dopamine in the dorsal striatum and methylphenidate amplifies this effectNora D Volkow
Medical Department, Brookhaven National Laboratory, Upton, New York 11973, USA
Synapse 44:175-80. 2002..In addition it demonstrates the ability of methylphenidate to amplify weak dopamine signals... - Toluene inhalation produces a conditioned place preference in ratsMadina R Gerasimov
Chemistry Department, Brookhaven National Laboratory, Upton, NY 11973, USA
Eur J Pharmacol 477:45-52. 2003..Thus, toluene produced a clear conditioned place preference that appears to be most evident when animals are not intoxicated. This procedure should be useful for further studies of the abuse-related effects of abused inhalants... - Preparation of radioiodinated peptide nucleic acids with high specific activityAdriana Tovar Salazar
Medical Department, Brookhaven National Laboratory, Upton, NY 11973, USA
Anal Biochem 360:92-8. 2007..As an example of a potential molecular biology application of the resultant (125)I-labeled PNA probe, it was used to detect mRNA for the Lcn2 gene in Northern blotting... - Conditioned place preference to morphine in cannabinoid CB1 receptor knockout miceOnarae V Rice
Medical Department, Brookhaven National Laboratory, Upton, NY 11973, USA
Brain Res 945:135-8. 2002..CB1 receptor knockout mice developed a strong place preference to 4 and 8 mg/kg morphine, similar to that in wild-type Swiss-Webster mice. This data thus does not support a contribution of the brain cannabinoid system to morphine reward... - Potent cannabinergic indole analogues as radioiodinatable brain imaging agents for the CB1 cannabinoid receptorHongfeng Deng
Center for Drug Discovery, Northeastern University, 360 Huntington Avenue, 116 Mugar Life Sciences Building, Boston, Massachusetts 02115, USA
J Med Chem 48:6386-92. 2005..Radioiodinated 8 thus appears to be a suitable radioligand for studies of CB1 cannabinoid receptors...
Research Grants
- MODULATION OF NEUROTRANSMITTER RELEASE BY CANNABINOIDSAndrew Gifford; Fiscal Year: 2002..Furthermore, these experiments will give information on the mode of action of cannabinoid antagonists and could suggest novel therapeutic uses for this new class of compounds. ..
- Animal model for inhalant abuseAndrew Gifford; Fiscal Year: 2004..Additionally, an animal model for inhalant abuse will aid further exploration of the factors contributing to the predisposition, development and potential treatment of inhalant abuse. ..
- PET Studies of Alcohol Distribution and MetabolismAndrew Gifford; Fiscal Year: 2005..abstract_text> ..