M Rosbash

Summary

Affiliation: Brandeis University
Country: USA

Publications

  1. ncbi request reprint Yeast pre-mRNA is composed of two populations with distinct kinetic properties
    D J Elliott
    Department of Biology, Brandeis University, Waltham, Massachusetts, 02254, USA
    Exp Cell Res 229:181-8. 1996
  2. ncbi request reprint Drosophila CRY is a deep brain circadian photoreceptor
    P Emery
    Department of Biology, National Science Foundation Center for Biological Timing, Waltham, Massachusetts 02454, USA
    Neuron 26:493-504. 2000
  3. ncbi request reprint The cryb mutation identifies cryptochrome as a circadian photoreceptor in Drosophila
    R Stanewsky
    Department of Biology and National Science Foundation Center for Biological Timing, Brandeis University, Waltham, Massachusetts 02454, USA
    Cell 95:681-92. 1998
  4. ncbi request reprint CYCLE is a second bHLH-PAS clock protein essential for circadian rhythmicity and transcription of Drosophila period and timeless
    J E Rutila
    NSF, Center for Biological Timing, and Department of Biology, Brandeis University, Waltham, Massachusetts 02254, USA
    Cell 93:805-14. 1998
  5. pmc Identification of novel Saccharomyces cerevisiae proteins with nuclear export activity: cell cycle-regulated transcription factor ace2p shows cell cycle-independent nucleocytoplasmic shuttling
    T H Jensen
    Howard Hughes Medical Institute, Department of Biology, Brandeis University, Waltham, Massachusetts 02454, USA
    Mol Cell Biol 20:8047-58. 2000
  6. pmc TIMELESS-dependent positive and negative autoregulation in the Drosophila circadian clock
    V Suri
    Department of Biochemistry, Howard Hughes Medical Institute, 415 South Street, Brandeis University, Waltham, MA 02454, USA
    EMBO J 18:675-86. 1999
  7. pmc Identification and characterization of a yeast gene encoding the U2 small nuclear ribonucleoprotein particle B" protein
    J Tang
    Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02254, USA
    Mol Cell Biol 16:2787-95. 1996
  8. pmc takeout, a novel Drosophila gene under circadian clock transcriptional regulation
    W V So
    Howard Hughes Medical Institute, NSF Center for Biological Timing, Department of Biology, Brandeis University, Waltham, Massachusetts 02454, USA
    Mol Cell Biol 20:6935-44. 2000
  9. ncbi request reprint CRY, a Drosophila clock and light-regulated cryptochrome, is a major contributor to circadian rhythm resetting and photosensitivity
    P Emery
    Department of Biology, Brandeis University Waltham, Massachusetts 02454, USA
    Cell 95:669-79. 1998
  10. ncbi request reprint Two novel doubletime mutants alter circadian properties and eliminate the delay between RNA and protein in Drosophila
    V Suri
    Graduate Departments of Biochemistry and Biology, Howard Hughes Medical Institute, and National Science Foundation Center for Biological Timing, Brandeis University, Waltham, Massachusetts 02454, USA
    J Neurosci 20:7547-55. 2000

Collaborators

Detail Information

Publications50

  1. ncbi request reprint Yeast pre-mRNA is composed of two populations with distinct kinetic properties
    D J Elliott
    Department of Biology, Brandeis University, Waltham, Massachusetts, 02254, USA
    Exp Cell Res 229:181-8. 1996
    ..As a result of its comparatively long lifetime, this minor fraction of the pre-mRNA population makes up the majority of the steady-state level of GalRP51A pre-mRNA...
  2. ncbi request reprint Drosophila CRY is a deep brain circadian photoreceptor
    P Emery
    Department of Biology, National Science Foundation Center for Biological Timing, Waltham, Massachusetts 02454, USA
    Neuron 26:493-504. 2000
    ..These brain neurons therefore contain an identified deep brain photoreceptor, as well as the other circadian elements: a central pace-maker and a behavioral output system...
  3. ncbi request reprint The cryb mutation identifies cryptochrome as a circadian photoreceptor in Drosophila
    R Stanewsky
    Department of Biology and National Science Foundation Center for Biological Timing, Brandeis University, Waltham, Massachusetts 02454, USA
    Cell 95:681-92. 1998
    ..cryb flies are rhythmic in constant darkness, correlating with robust PER and TIM cycling in certain pacemaker neurons...
  4. ncbi request reprint CYCLE is a second bHLH-PAS clock protein essential for circadian rhythmicity and transcription of Drosophila period and timeless
    J E Rutila
    NSF, Center for Biological Timing, and Department of Biology, Brandeis University, Waltham, Massachusetts 02254, USA
    Cell 93:805-14. 1998
    ..We propose that the CYC:CLK heterodimer binds to per and tim E boxes and makes a major contribution to the circadian transcription of Drosophila clock genes...
  5. pmc Identification of novel Saccharomyces cerevisiae proteins with nuclear export activity: cell cycle-regulated transcription factor ace2p shows cell cycle-independent nucleocytoplasmic shuttling
    T H Jensen
    Howard Hughes Medical Institute, Department of Biology, Brandeis University, Waltham, Massachusetts 02454, USA
    Mol Cell Biol 20:8047-58. 2000
    ..This feature of Ace2p localization explains its ability to activate genes such as CUP1, which are not expressed in a cell cycle-dependent manner...
  6. pmc TIMELESS-dependent positive and negative autoregulation in the Drosophila circadian clock
    V Suri
    Department of Biochemistry, Howard Hughes Medical Institute, 415 South Street, Brandeis University, Waltham, MA 02454, USA
    EMBO J 18:675-86. 1999
    ..Our results suggest positive as well as negative autoregulation in the Drosophila circadian clock...
  7. pmc Identification and characterization of a yeast gene encoding the U2 small nuclear ribonucleoprotein particle B" protein
    J Tang
    Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02254, USA
    Mol Cell Biol 16:2787-95. 1996
    ..Surprisingly, the YU2B" protein lacks a C-terminal RNA binding domain, which is conserved in all other family members. Possible functional and evolutionary relationships among these proteins are discussed...
  8. pmc takeout, a novel Drosophila gene under circadian clock transcriptional regulation
    W V So
    Howard Hughes Medical Institute, NSF Center for Biological Timing, Department of Biology, Brandeis University, Waltham, Massachusetts 02454, USA
    Mol Cell Biol 20:6935-44. 2000
    ..The circadian delayed transcriptional phase is therefore most likely the result of indirect regulation through unknown transcription factors...
  9. ncbi request reprint CRY, a Drosophila clock and light-regulated cryptochrome, is a major contributor to circadian rhythm resetting and photosensitivity
    P Emery
    Department of Biology, Brandeis University Waltham, Massachusetts 02454, USA
    Cell 95:669-79. 1998
    ..Taken together with the data in the accompanying paper, we propose that CRY is a major Drosophila photoreceptor dedicated to the resetting of circadian rhythms...
  10. ncbi request reprint Two novel doubletime mutants alter circadian properties and eliminate the delay between RNA and protein in Drosophila
    V Suri
    Graduate Departments of Biochemistry and Biology, Howard Hughes Medical Institute, and National Science Foundation Center for Biological Timing, Brandeis University, Waltham, Massachusetts 02454, USA
    J Neurosci 20:7547-55. 2000
    ....
  11. pmc Wild-type circadian rhythmicity is dependent on closely spaced E boxes in the Drosophila timeless promoter
    M J McDonald
    Department of Biology, National Science Foundation Center for Biological Timing, Waltham, Massachusetts 02454, USA
    Mol Cell Biol 21:1207-17. 2001
    ..These E-box-like sequences cooperate with the canonical E-box element to promote high-amplitude transcription, which is necessary for wild-type rhythmicity...
  12. pmc The NES-Crm1p export pathway is not a major mRNA export route in Saccharomyces cerevisiae
    M Neville
    Howard Hughes Medical Institute, Department of Biology, MS008 Brandeis University, Waltham, MA 02454, USA
    EMBO J 18:3746-56. 1999
    ..cerevisiae. We conclude that Crm1p structure and function is conserved from S.cerevisiae to man...
  13. ncbi request reprint A mutant Drosophila homolog of mammalian Clock disrupts circadian rhythms and transcription of period and timeless
    R Allada
    NSF, Center for Biological Timing, and Department of Biology, Brandeis University, Waltham, Massachusetts 02254, USA
    Cell 93:791-804. 1998
    ..The remarkable sequence conservation strongly supports common clock components present in the common ancestor of Drosophila and mammals...
  14. pmc A new gene encoding a putative transcription factor regulated by the Drosophila circadian clock
    F Rouyer
    HHMI and Department of Biology, Brandeis University, Waltham, MA 02254, USA
    EMBO J 16:3944-54. 1997
    ..In the adult head, in situ hybridization analysis reveals that per and Crg-1 have similar expression patterns in the eyes and optic lobes...
  15. ncbi request reprint The importin-beta family member Crm1p bridges the interaction between Rev and the nuclear pore complex during nuclear export
    M Neville
    Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02254, USA
    Curr Biol 7:767-75. 1997
    ..Crm1p has recently been shown to contribute to nuclear export in vertebrate systems. Here, we have studied this mechanism of nuclear to cytoplasmic transport...
  16. ncbi request reprint Evidence that the TIM light response is relevant to light-induced phase shifts in Drosophila melanogaster
    V Suri
    Department of Biology, Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02254, USA
    Neuron 21:225-34. 1998
    ..Analysis of dose response of TIM disappearance in a variety of mutant genotypes suggests cell-autonomous light responses that are largely independent of the canonical visual transduction pathway...
  17. ncbi request reprint Cell biology. TAPping into mRNA export
    M J Moore
    Department of Biochemistry, Howard Hughes Medical Institute, Brandeis University, Waltham, MA 02454, USA
    Science 294:1841-2. 2001
    ..In a Perspective, Moore and Rosbash discuss a new strategy using cell-penetrating peptide inhibitors for unraveling the routes of mRNA export in living cells (Gallouzi and Steitz)...
  18. ncbi request reprint Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals
    N Abovich
    Howard Hughes Medical Institute, Department of Biology, Brandeis University, Waltham, Massachusetts 02254, USA
    Cell 89:403-12. 1997
    ..Our results show that SF1 interacts strongly with human U2AF65, and that SF1 is a bona fide E complex component. This implies that aspects of these novel cross-intron protein-protein interactions are conserved between yeast and mammals...
  19. pmc Differential regulation of circadian pacemaker output by separate clock genes in Drosophila
    J H Park
    Department of Biology and National Science Foundation Center for Biological Timing, and Howard Hughes Medical Institute, Brandeis University, Waltham, MA 02454, USA
    Proc Natl Acad Sci U S A 97:3608-13. 2000
    ....
  20. ncbi request reprint The yeast splicing factor Mud13p is a commitment complex component and corresponds to CBP20, the small subunit of the nuclear cap-binding complex
    H V Colot
    Howard Hughes Medical Institute, Brandeis University, Department of Biology, Waltham, Massachusetts 02254, USA
    Genes Dev 10:1699-708. 1996
    ..Taken together with the accompanying results for a mammalian system, our data indicate that cap-binding proteins as well as the pre-mRNA cap contribute to early steps in spliceosome assembly...
  21. ncbi request reprint A biochemical function for the Sm complex
    D Zhang
    Howard Hughes Medical Institute, Department of Biology, Brandeis University, Waltham, MA 02254, USA
    Mol Cell 7:319-29. 2001
    ..We speculate that this role may be general, and may have even evolved to aid weak intermolecular nucleic acid interactions of only a few base pairs...
  22. ncbi request reprint Temporal and spatial expression patterns of transgenes containing increasing amounts of the Drosophila clock gene period and a lacZ reporter: mapping elements of the PER protein involved in circadian cycling
    R Stanewsky
    Department of Biology, Brandeis University, Waltham, Massachusetts 02254, USA
    J Neurosci 17:676-96. 1997
    ..The XLG construct restored weak behavioral rhythmicity to (otherwise) per01 files, indicating that the C-terminal third of PER (missing in BG) is necessary to fulfill the biological function of this clock protein...
  23. pmc Post-transcriptional regulation contributes to Drosophila clock gene mRNA cycling
    W V So
    Howard Hughes Medical Institute, Department of Biology, Brandeis University, 415 South Street, Waltham, MA 02254, USA
    EMBO J 16:7146-55. 1997
    ..The data show that circadian mRNA oscillations can take place without evident transcriptional regulation...
  24. pmc Characterization of yeast U1 snRNP A protein: identification of the N-terminal RNA binding domain (RBD) binding site and evidence that the C-terminal RBD functions in splicing
    J Tang
    Howard Hughes Medical Institute and Department of Biology, Brandeis University, Waltham, Massachusetts 02254, USA
    RNA 2:1058-70. 1996
    ..We propose that the N-terminal RBD serves to tether the splicing-relevant C-terminal RBD to the snRNP...
  25. ncbi request reprint The Drosophila takeout gene is a novel molecular link between circadian rhythms and feeding behavior
    L Sarov-Blat
    Howard Hughes Medical Institute, National Science Foundation, Department of Biology, Brandeis University, Waltham, Massachusetts 02454, USA
    Cell 101:647-56. 2000
    ..We propose that to participates in a novel circadian output pathway that conveys temporal and food status information to feeding-relevant metabolisms and activities...
  26. pmc A cooperative interaction between U2AF65 and mBBP/SF1 facilitates branchpoint region recognition
    J A Berglund
    Howard Hughes Medical Institute and Departments of Biology and Biochemistry, Brandeis University, Waltham, Massachusetts 02254, USA
    Genes Dev 12:858-67. 1998
    ..We also demonstrate the essential nature of the third RBD of U2AF65 for the interaction between the two proteins, both in the presence and absence of RNA...
  27. ncbi request reprint A unique circadian-rhythm photoreceptor
    P Emery
    Department of Biology, NSF Center for Biological Timing, Brandeis University, Waltham, Massachusetts 02454, USA
    Nature 404:456-7. 2000
  28. ncbi request reprint Circadian cycling of a PERIOD-beta-galactosidase fusion protein in Drosophila: evidence for cyclical degradation
    M E Dembinska
    Howard Hughes Medical Institute, Brandeis University, Waltham, MA 02254, USA
    J Biol Rhythms 12:157-72. 1997
    ..The results are discussed with respect to the posttranscriptional regulation that is necessary for proper cycling of both PER and TIM as well as for clock function...
  29. pmc The yeast nucleoporin rip1p contributes to multiple export pathways with no essential role for its FG-repeat region
    F Stutz
    Howard Hughes Medical Institute and Department of Biology, Brandeis University, Waltham, Massachusetts 02254, USA
    Genes Dev 11:2857-68. 1997
    ..Finally, this domain of Rip1p also contributes to Rev-mediated RNA export. The data suggest that Rip1p promotes the nuclear export of different classes of substrates by contributing to optimal pore function...
  30. ncbi request reprint The timSL mutant affects a restricted portion of the Drosophila melanogaster circadian cycle
    J E Rutila
    Howard Hughes Medical Institute, Brandeis University, Department of Biology, Waltham, MA 02454 9110, USA
    J Biol Rhythms 13:380-92. 1998
    ..The authors propose that TIM and PER phosphorylation are normally rate determining during the mid-late night region of the circadian cycle...
  31. ncbi request reprint Some cis- and trans-acting mutants for splicing target pre-mRNA to the cytoplasm
    P Legrain
    Department of Biology, Brandeis University, Waltham, Massachusetts 02254
    Cell 57:573-83. 1989
    ....
  32. ncbi request reprint A block to mRNA nuclear export in S. cerevisiae leads to hyperadenylation of transcripts that accumulate at the site of transcription
    T H Jensen
    Howard Hughes Medical Institute, Department of Biology, Brandeis University, Waltham, MA 02454, USA
    Mol Cell 7:887-98. 2001
    ..Poly(A) tails of the transcripts are also dramatically longer in all of these export mutants. Based on all of the data, we suggest that very early mRNA maturation events determine transcript export competence...
  33. ncbi request reprint A promoterless period gene mediates behavioral rhythmicity and cyclical per expression in a restricted subset of the Drosophila nervous system
    B Frisch
    Department of Biology, Brandeis University, Waltham, Massachusetts 02254
    Neuron 12:555-70. 1994
    ..Cycling of PER in brain cells of flies carrying the same 7.2 kb piece of per DNA under the control of a heat shock promoter corroborated the hypothesis that per's molecular cyclings and behavioral rhythmicity are causally related...
  34. ncbi request reprint An enhancer screen identifies a gene that encodes the yeast U1 snRNP A protein: implications for snRNP protein function in pre-mRNA splicing
    X C Liao
    Howard Hughes Medical Institute, Department of Biology, Brandeis University, Waltham, Massachusetts 02254
    Genes Dev 7:419-28. 1993
    ..Even in vitro, the absence of the protein has little effect on splicing. On the basis of these observations, we suggest that a principal role of the U1A protein is to help fold or maintain U1 RNA in an active configuration...
  35. ncbi request reprint Phase shifting of the circadian clock by induction of the Drosophila period protein
    I Edery
    Howard Hughes Medical Institute, Department of Biology, Brandeis University, Waltham, MA 02254
    Science 263:237-40. 1994
    ..This treatment caused long-lasting phase shifts in the locomotor activity circadian rhythm, a result that supports the contention that PER is a bona fide clock component...
  36. ncbi request reprint Identification of a novel nuclear pore-associated protein as a functional target of the HIV-1 Rev protein in yeast
    F Stutz
    Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02254, USA
    Cell 82:495-506. 1995
    ..The results suggest that Rev directly promotes the cytoplasmic transport of suitable transcripts by targeting them to the nuclear pore...
  37. pmc Yeast U1 snRNP-pre-mRNA complex formation without U1snRNA-pre-mRNA base pairing
    H Du
    Department of Biology, Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02454, USA
    RNA 7:133-42. 2001
    ..Only one of these is base pairing between the 5' splice site and the 5' end of U1 snRNA, without which the U1 snRNP-pre-mRNA complex is less stable and has a somewhat altered conformation...
  38. ncbi request reprint Microarray analysis and organization of circadian gene expression in Drosophila
    M J McDonald
    Department of Biology, National Science Foundation Center for Biological Timing, Waltham, MA 02454, USA
    Cell 107:567-78. 2001
    ..As we identified a small number of direct target genes, the data suggest that most of the circadian gene network is indirectly regulated by clk...
  39. ncbi request reprint The splicing factor BBP interacts specifically with the pre-mRNA branchpoint sequence UACUAAC
    J A Berglund
    Howard Hughes Medical Institute, Department of Biology and Biochemistry, Brandeis University, Waltham, Massachusetts 02254, USA
    Cell 89:781-7. 1997
    ..The relative conservation of this region in yeast and mammals correlates well with the RNA-binding differences between BBP and mBBP, suggesting that BBP contributes to branchpoint sequence definition in both systems...
  40. pmc Effect of constant light and circadian entrainment of perS flies: evidence for light-mediated delay of the negative feedback loop in Drosophila
    S B Marrus
    Howard Hughes Medical Institute, Department of Biology, Brandeis University, Waltham, MA 02254, USA
    EMBO J 15:6877-86. 1996
    ..A standard 24 h light-dark cycle delays the negative feedback circuit and extends the RNA and protein profiles, compensating for the accelerated RNA increase and restoring the rhythms to wild-type-like periodicity...
  41. ncbi request reprint A Drosophila circadian clock
    M Rosbash
    Howard Hughes Medical Institute, National Science Foundation Science and Technology Center for Biological Timing, Department of Biology, Brandeis University, Waltham, Massachusetts 02254, USA
    Cold Spring Harb Symp Quant Biol 61:265-78. 1996
  42. pmc Identification of eight proteins that cross-link to pre-mRNA in the yeast commitment complex
    D Zhang
    Department of Biology, Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02454, USA
    Genes Dev 13:581-92. 1999
    ....
  43. doi request reprint Transcriptional feedback and definition of the circadian pacemaker in Drosophila and animals
    M Rosbash
    Biology Department, Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02454, USA
    Cold Spring Harb Symp Quant Biol 72:75-83. 2007
    ..We propose that cyanobacteria and animal clocks reflect two independent origins of circadian rhythms...
  44. pmc Temporal phosphorylation of the Drosophila period protein
    I Edery
    Howard Hughes Medical Institute, Department of Biology, Brandeis University, Waltham, MA 02254
    Proc Natl Acad Sci U S A 91:2260-4. 1994
    ..We suggest that the phosphorylation status of PER is an important determinant in the Drosophila clock's time-keeping mechanism...
  45. ncbi request reprint PAS is a dimerization domain common to Drosophila period and several transcription factors
    Z J Huang
    Howard Hughes Medical Institute, Department of Biology, Brandeis University, Waltham, Massachusetts 02254
    Nature 364:259-62. 1993
    ....
  46. pmc Oscillating molecules and how they move circadian clocks across evolutionary boundaries
    J C Hall
    Department of Biology, Brandeis University, Waltham, MA 02254
    Proc Natl Acad Sci U S A 90:5382-3. 1993
  47. ncbi request reprint The DECD box putative ATPase Sub2p is an early mRNA export factor
    T H Jensen
    Howard Hughes Medical Institute, Department of Biology, Brandeis University, Waltham, MA 02454, USA
    Curr Biol 11:1711-5. 2001
    ..Taken together, our data suggest that Sub2p functions at an early step in the mRNA export process...
  48. pmc A role for nucleoporin FG repeat domains in export of human immunodeficiency virus type 1 Rev protein and RNA from the nucleus
    F Stutz
    Department of Biology, Howard Hughes Medical Institute, Brandeis University, Waltham, Massachusetts 02254, USA
    Mol Cell Biol 16:7144-50. 1996
    ..The results suggest that Rev and cellular mediators of RNA export can interact with multiple components of the nuclear pore complex during transport, analogous to the proposed mode of action of the nuclear protein import receptor...
  49. ncbi request reprint The yeast MUD2 protein: an interaction with PRP11 defines a bridge between commitment complexes and U2 snRNP addition
    N Abovich
    Howard Hughes Medical Institute, Brandeis University, Department of Biology, Waltham, Massachusetts 02254
    Genes Dev 8:843-54. 1994
    ..The experiments identify a specific inter-snRNP protein-protein contact that occurs during spliceosome assembly and more generally support substantial functional similarity between U2AF65 and MUD2P...
  50. pmc Constitutive overexpression of the Drosophila period protein inhibits period mRNA cycling
    H Zeng
    Howard Hughes Medical Institute, Department of Biology, Brandeis University, Waltham, MA 02254
    EMBO J 13:3590-8. 1994
    ..These results indicate that the autoregulation of per expression is a direct, intracellular event and suggest that each per-expressing cell contains an autonomous oscillator of which the per feedback loop is a component...