K E Pierce

Summary

Affiliation: Brandeis University
Country: USA

Publications

  1. doi request reprint Rapid detection and identification of hepatitis C virus (HCV) sequences using mismatch-tolerant hybridization probes: A general method for analysis of sequence variation
    Kenneth E Pierce
    Department of Biology, Brandeis University, Waltham, MA, USA
    Biotechniques 55:125-32. 2013
  2. doi request reprint Rapid detection of TEM-type extended-spectrum β-lactamase (ESBL) mutations using lights-on/lights-off probes with single-stranded DNA amplification
    Kenneth E Pierce
    Department of Biology, Brandeis University, Waltham, MA 02454, USA
    J Mol Diagn 15:291-8. 2013
  3. doi request reprint Rapid detection of sequence variation in Clostridium difficile genes using LATE-PCR with multiple mismatch-tolerant hybridization probes
    Kenneth E Pierce
    Department of Biology, Brandeis University, Waltham, MA 02454 9110, USA
    J Microbiol Methods 91:269-75. 2012
  4. pmc Two-temperature LATE-PCR endpoint genotyping
    J Aquiles Sanchez
    Department of Biology, MS008, Brandeis University, Waltham, MA 02454, USA
    BMC Biotechnol 6:44. 2006
  5. pmc Linear-After-The-Exponential (LATE)-PCR: primer design criteria for high yields of specific single-stranded DNA and improved real-time detection
    Kenneth E Pierce
    Department of Biology, Brandeis University, Waltham, MA 02454 9110, USA
    Proc Natl Acad Sci U S A 102:8609-14. 2005
  6. ncbi request reprint Effectiveness and limitations of uracil-DNA glycosylases in sensitive real-time PCR assays
    Kenneth E Pierce
    Department of Biology MS 008, Brandeis University, Waltham, MA 02454 9110, USA
    Biotechniques 36:44-6, 48. 2004
  7. ncbi request reprint Detection of cystic fibrosis alleles from single cells using molecular beacons and a novel method of asymmetric real-time PCR
    Kenneth E Pierce
    Department of Biology, MS 008, Brandeis University, Waltham, MA 02454 9110, USA
    Mol Hum Reprod 9:815-20. 2003
  8. doi request reprint LATE-PCR and allied technologies: real-time detection strategies for rapid, reliable diagnosis from single cells
    Kenneth E Pierce
    Department of Biology, Brandeis University, Waltham, MA, USA
    Methods Mol Biol 688:47-66. 2011
  9. doi request reprint Design and optimization of a novel reverse transcription linear-after-the-exponential PCR for the detection of foot-and-mouth disease virus
    K E Pierce
    Department of Biology, Brandeis University, Waltham, MA 02454, USA
    J Appl Microbiol 109:180-9. 2010
  10. pmc Linear-after-the-exponential (LATE)-PCR: an advanced method of asymmetric PCR and its uses in quantitative real-time analysis
    J Aquiles Sanchez
    Department of Biology, MS 008, Brandeis University, 415 South Street, Waltham, MA 02454 9110, USA
    Proc Natl Acad Sci U S A 101:1933-8. 2004

Collaborators

Detail Information

Publications15

  1. doi request reprint Rapid detection and identification of hepatitis C virus (HCV) sequences using mismatch-tolerant hybridization probes: A general method for analysis of sequence variation
    Kenneth E Pierce
    Department of Biology, Brandeis University, Waltham, MA, USA
    Biotechniques 55:125-32. 2013
    ..This assay can be expanded to include additional gene segments, and it points the way to construction of highly informative single-tube assays for HCV and other RNA viruses. ..
  2. doi request reprint Rapid detection of TEM-type extended-spectrum β-lactamase (ESBL) mutations using lights-on/lights-off probes with single-stranded DNA amplification
    Kenneth E Pierce
    Department of Biology, Brandeis University, Waltham, MA 02454, USA
    J Mol Diagn 15:291-8. 2013
    ..Clinical use of this convenient, single-closed-tube assay would make it possible to rapidly distinguish ESBL from non-ESBL variants and thereby to begin early treatment with suitable antibiotics...
  3. doi request reprint Rapid detection of sequence variation in Clostridium difficile genes using LATE-PCR with multiple mismatch-tolerant hybridization probes
    Kenneth E Pierce
    Department of Biology, Brandeis University, Waltham, MA 02454 9110, USA
    J Microbiol Methods 91:269-75. 2012
    ..This single closed-tube assay generates substantially more information about virulent C. difficile than currently available commercial assays and could be further expanded to provide strain typing...
  4. pmc Two-temperature LATE-PCR endpoint genotyping
    J Aquiles Sanchez
    Department of Biology, MS008, Brandeis University, Waltham, MA 02454, USA
    BMC Biotechnol 6:44. 2006
    ..Furthermore, the use of only a single colored probe for genotyping enhances the multiplex detection capacity of the assay...
  5. pmc Linear-After-The-Exponential (LATE)-PCR: primer design criteria for high yields of specific single-stranded DNA and improved real-time detection
    Kenneth E Pierce
    Department of Biology, Brandeis University, Waltham, MA 02454 9110, USA
    Proc Natl Acad Sci U S A 102:8609-14. 2005
    ..The conclusions were validated by using primer pairs that amplify sequences within the cystic fibrosis transmembrane regulator (CFTR) gene, mutations of which are responsible for cystic fibrosis...
  6. ncbi request reprint Effectiveness and limitations of uracil-DNA glycosylases in sensitive real-time PCR assays
    Kenneth E Pierce
    Department of Biology MS 008, Brandeis University, Waltham, MA 02454 9110, USA
    Biotechniques 36:44-6, 48. 2004
  7. ncbi request reprint Detection of cystic fibrosis alleles from single cells using molecular beacons and a novel method of asymmetric real-time PCR
    Kenneth E Pierce
    Department of Biology, MS 008, Brandeis University, Waltham, MA 02454 9110, USA
    Mol Hum Reprod 9:815-20. 2003
    ..4%), and contamination among homozygous DeltaF508 lymphoblasts, 1/57 (1.8%). LATE-PCR offers a new strategy for preimplantation genetic diagnosis and other fields in which accurate quantitative detection of single copy genes is important...
  8. doi request reprint LATE-PCR and allied technologies: real-time detection strategies for rapid, reliable diagnosis from single cells
    Kenneth E Pierce
    Department of Biology, Brandeis University, Waltham, MA, USA
    Methods Mol Biol 688:47-66. 2011
    ..The analysis of real-time kinetics of LATE-PCR signals provides a means for improving the accuracy of single-cell genetic diagnosis...
  9. doi request reprint Design and optimization of a novel reverse transcription linear-after-the-exponential PCR for the detection of foot-and-mouth disease virus
    K E Pierce
    Department of Biology, Brandeis University, Waltham, MA 02454, USA
    J Appl Microbiol 109:180-9. 2010
    ..A novel molecular assay for the detection of foot-and-mouth disease virus (FMDV) was developed using linear-after-the-exponential polymerase chain reaction (LATE-PCR)...
  10. pmc Linear-after-the-exponential (LATE)-PCR: an advanced method of asymmetric PCR and its uses in quantitative real-time analysis
    J Aquiles Sanchez
    Department of Biology, MS 008, Brandeis University, 415 South Street, Waltham, MA 02454 9110, USA
    Proc Natl Acad Sci U S A 101:1933-8. 2004
    ..We showcase LATE-PCR via amplification of the cystic fibrosis CFDelta508 allele and the Tay-Sachs disease TSD 1278 allele from single heterozygous cells...
  11. ncbi request reprint Real-time PCR with molecular beacons provides a highly accurate assay for detection of Tay-Sachs alleles in single cells
    John E Rice
    Department of Biology, Brandeis University, Waltham, MA 02454 9110, USA
    Prenat Diagn 22:1130-4. 2002
    ..Only two of the 135 heterozygous samples were allele drop-outs, a rate far lower than previously reported for single-cell Tay-Sachs assays using conventional methods of PCR...
  12. ncbi request reprint Monoplex/multiplex linear-after-the-exponential-PCR assays combined with PrimeSafe and Dilute-'N'-Go sequencing
    John E Rice
    Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Nat Protoc 2:2429-38. 2007
    ..The preparation of LATE-PCR products for Dilute-'N'-Go sequencing takes only 30 seconds...
  13. ncbi request reprint Linear-after-the-exponential polymerase chain reaction and allied technologies. Real-time detection strategies for rapid, reliable diagnosis from single cells
    Kenneth E Pierce
    Department of Biology, Brandeis University, Waltham, MA, USA
    Methods Mol Med 132:65-85. 2007
    ..The analysis of real-time kinetics of LATE-PCR signals provides a means for improving the accuracy of single cell genetic diagnosis...
  14. pmc Direct amplification of single-stranded DNA for pyrosequencing using linear-after-the-exponential (LATE)-PCR
    Jesse J Salk
    Department of Biology, Brandeis University, Waltham, MA 02454, USA
    Anal Biochem 353:124-32. 2006
    ....
  15. ncbi request reprint QuantiLyse: reliable DNA amplification from single cells
    Kenneth E Pierce
    Brandeis University, Waltham, MA 02454 9110, USA
    Biotechniques 32:1106-11. 2002
    ..QuantiLyse will be useful in a range of investigations aimed at gene detection in small numbers of cells...