J E Haber

Summary

Affiliation: Brandeis University
Country: USA

Publications

  1. pmc Lack of chromosome territoriality in yeast: promiscuous rejoining of broken chromosome ends
    J E Haber
    Rosenstiel Center, Brandeis University, Waltham, MA 02254 9110, USA
    Proc Natl Acad Sci U S A 93:13949-54. 1996
  2. ncbi request reprint Meiosis. Searching for a partner
    J E Haber
    Rosenstiel Center, Department of Biology, Brandeis University, Waltham, MA 02254 9110, USA
    Science 279:823-4. 1998
  3. ncbi request reprint A locus control region regulates yeast recombination
    J E Haber
    Rosenstiel Center, Keck Institute for Cellular Visualization, Brandeis University, Waltham, MA 02254, USA
    Trends Genet 14:317-21. 1998
  4. ncbi request reprint Meiosis: Avoiding inappropriate relationships
    J E Haber
    Rosenstiel Center MS029, Brandeis University, Waltham, Massachusetts 02454 9110 USA
    Curr Biol 8:R832-5. 1998
  5. ncbi request reprint Mating-type gene switching in Saccharomyces cerevisiae
    J E Haber
    Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Annu Rev Genet 32:561-99. 1998
  6. ncbi request reprint The Saccharomyces recombination protein Tid1p is required for adaptation from G2/M arrest induced by a double-strand break
    S E Lee
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02454, USA
    Curr Biol 11:1053-7. 2001
  7. pmc Genetic requirements for RAD51- and RAD54-independent break-induced replication repair of a chromosomal double-strand break
    L Signon
    Department of Biology and Rosenstiel Center, Brandeis University, Waltham, Massachusetts 02254 9110, USA
    Mol Cell Biol 21:2048-56. 2001
  8. pmc Multiple pathways of recombination induced by double-strand breaks in Saccharomyces cerevisiae
    F Pâques
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Microbiol Mol Biol Rev 63:349-404. 1999
  9. pmc Removal of one nonhomologous DNA end during gene conversion by a RAD1- and MSH2-independent pathway
    M P Colaiácovo
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Genetics 151:1409-23. 1999
  10. pmc Two pathways for removal of nonhomologous DNA ends during double-strand break repair in Saccharomyces cerevisiae
    F Pâques
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02254 9110, USA
    Mol Cell Biol 17:6765-71. 1997

Collaborators

Detail Information

Publications90

  1. pmc Lack of chromosome territoriality in yeast: promiscuous rejoining of broken chromosome ends
    J E Haber
    Rosenstiel Center, Brandeis University, Waltham, MA 02254 9110, USA
    Proc Natl Acad Sci U S A 93:13949-54. 1996
    ....
  2. ncbi request reprint Meiosis. Searching for a partner
    J E Haber
    Rosenstiel Center, Department of Biology, Brandeis University, Waltham, MA 02254 9110, USA
    Science 279:823-4. 1998
  3. ncbi request reprint A locus control region regulates yeast recombination
    J E Haber
    Rosenstiel Center, Keck Institute for Cellular Visualization, Brandeis University, Waltham, MA 02254, USA
    Trends Genet 14:317-21. 1998
    ..Donor preference is regulated by a 250 bp recombination enhancer, that controls recombination of the entire left arm of chromosome III. Recent studies have shown how this locus-control region is turned on and off...
  4. ncbi request reprint Meiosis: Avoiding inappropriate relationships
    J E Haber
    Rosenstiel Center MS029, Brandeis University, Waltham, Massachusetts 02454 9110 USA
    Curr Biol 8:R832-5. 1998
    ..Recent studies with the yeast Saccharomyces cerevisiae have identified some of the key players that link homologous recombination to synaptonemal complex formation...
  5. ncbi request reprint Mating-type gene switching in Saccharomyces cerevisiae
    J E Haber
    Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Annu Rev Genet 32:561-99. 1998
    ..In addition, MAT switching provides a remarkable example of a small locus control region--the Recombination Enhancer--that controls recombination along an entire chromosome arm...
  6. ncbi request reprint The Saccharomyces recombination protein Tid1p is required for adaptation from G2/M arrest induced by a double-strand break
    S E Lee
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02454, USA
    Curr Biol 11:1053-7. 2001
    ..We suggest that Tid1p participates in monitoring the extent of single-stranded DNA produced by resection of DNA ends in a fashion that is distinct from its role in recombination...
  7. pmc Genetic requirements for RAD51- and RAD54-independent break-induced replication repair of a chromosomal double-strand break
    L Signon
    Department of Biology and Rosenstiel Center, Brandeis University, Waltham, Massachusetts 02254 9110, USA
    Mol Cell Biol 21:2048-56. 2001
    ..The similar genetic requirements for BIR and telomere maintenance in the absence of telomerase also suggest that these two processes proceed by similar mechanisms...
  8. pmc Multiple pathways of recombination induced by double-strand breaks in Saccharomyces cerevisiae
    F Pâques
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Microbiol Mol Biol Rev 63:349-404. 1999
    ..This review encompasses these different aspects of DSB-induced recombination in Saccharomyces and attempts to relate genetic, molecular biological, and biochemical studies of the processes of DNA repair and recombination...
  9. pmc Removal of one nonhomologous DNA end during gene conversion by a RAD1- and MSH2-independent pathway
    M P Colaiácovo
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Genetics 151:1409-23. 1999
    ..We interpret these results in terms of the formation and resolution of alternative intermediates of a synthesis-dependent strand annealing mechanism...
  10. pmc Two pathways for removal of nonhomologous DNA ends during double-strand break repair in Saccharomyces cerevisiae
    F Pâques
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02254 9110, USA
    Mol Cell Biol 17:6765-71. 1997
    ..3' tails shorter than 30 nt are removed by another mechanism that depends at least in part on the 3'-to-5' proofreading activity of DNA polymerase delta...
  11. pmc Break-induced replication: a review and an example in budding yeast
    E Kraus
    Department of Biology and Rosenstiel Center, Brandeis University, Waltham, MA 02454 9110, USA
    Proc Natl Acad Sci U S A 98:8255-62. 2001
    ..Thus, the end of a linearized DNA fragment can initiate new DNA synthesis by BIR in which the newly synthesized DNA is displaced and subsequently forms circles by NHEJ...
  12. ncbi request reprint A Saccharomyces servazzii clone homologous to Saccharomyces cerevisiae chromosome III spanning KAR4, ARS 304 and SPB1 lacks the recombination enhancer but contains an unknown ORF
    Z Zhou
    Rosenstiel Center, Department of Biology, Brandeis University, Waltham, MA 02254-9110, USA
    Yeast 18:789-95. 2001
    ..cerevisiae. The S. servazzii region contains a sequence that supports autonomous DNA replication in S. cerevisiae and may represent a homologue of ARS304. The S. servazziii sequence has Genbank Accession No. BankIt359091 AF307954...
  13. pmc Mutations in XRS2 and RAD50 delay but do not prevent mating-type switching in Saccharomyces cerevisiae
    E L Ivanov
    Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts 02254 9110
    Mol Cell Biol 14:3414-25. 1994
    ..The XRS2 gene encodes an 854-amino-acid protein with no obvious similarity to the Rad50 protein or to any other protein in the database. Overexpression of RAD50 does not complement the defects in xrs2 or vice versa...
  14. ncbi request reprint NEJ1 controls non-homologous end joining in Saccharomyces cerevisiae
    M Valencia
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Nature 414:666-9. 2001
    ..Nej1 regulates the subcellular distribution of Lif1. A green fluorescent protein (GFP)-Lif1 fusion protein accumulates in the nucleus in cells expressing NEJ1 but is largely cytoplasmic when NEJ1 is repressed...
  15. pmc Expansions and contractions in 36-bp minisatellites by gene conversion in yeast
    F Pâques
    Rosenstiel Center and Department of Biology, Brandeis University, 415 South St, Waltham, MA 02454 9110, USA
    Genetics 158:155-66. 2001
    ..We conclude that the presence of nonhomologous ends alters the mechanism of DSB repair, likely through early recruitment of repair proteins including Msh2p and Rad1p, resulting in more frequent contractions of repeated sequences...
  16. pmc RAD51-independent break-induced replication to repair a broken chromosome depends on a distant enhancer site
    A Malkova
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02254-9110, USA
    Genes Dev 15:1055-60. 2001
    ..We conclude that the ability of a recombination-induced replication fork to copy > 130 kb to the end of the chromosome depends on a special site that enhances assembly of a processive repair replication fork...
  17. ncbi request reprint Role of yeast SIR genes and mating type in directing DNA double-strand breaks to homologous and non-homologous repair paths
    S E Lee
    Rosenstiel Center, Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Curr Biol 9:767-70. 1999
    ..Mating-type regulation of genes specific to haploid or diploid cells plays a key role in determining which pathways are used to repair DSBs...
  18. ncbi request reprint The fuss about Mus81
    J E Haber
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02254, USA
    Cell 107:551-4. 2001
    ..Mus81 proteins in yeasts and humans appear to have many, but not all, of the expected properties of eukaryotic Holliday junction resolvases, with intriguing connections to DNA replication checkpoints...
  19. pmc Two alternative pathways of double-strand break repair that are kinetically separable and independently modulated
    J Fishman-Lobell
    Rosenstiel Center, Brandeis University, Boston, Massachusetts 02254 9110
    Mol Cell Biol 12:1292-303. 1992
    ..4 kb. Moreover, the rate of deletion formation corresponds to the time at which complementary regions become single stranded. Gap repair processes are independent of distance but are reduced in rad52 mutants and in G1-arrested cells...
  20. pmc DNA length dependence of the single-strand annealing pathway and the role of Saccharomyces cerevisiae RAD59 in double-strand break repair
    N Sugawara
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Mol Cell Biol 20:5300-9. 2000
    ..A msh3 rad59 double mutant was more severely defective in SSA than either single mutant...
  21. pmc Role of Saccharomyces cerevisiae Msh2 and Msh3 repair proteins in double-strand break-induced recombination
    N Sugawara
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02254 9110, USA
    Proc Natl Acad Sci U S A 94:9214-9. 1997
    ..Mismatch repair proteins Msh6, Pms1, and Mlh1 are not required. We suggest Msh2 and Msh3 recognize not only heteroduplex loops and mismatched bp, but also branched DNA structures with a free 3' tail...
  22. pmc Genetic requirements for the single-strand annealing pathway of double-strand break repair in Saccharomyces cerevisiae
    E L Ivanov
    Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts 02254 9110, USA
    Genetics 142:693-704. 1996
    ....
  23. ncbi request reprint Lucky breaks: analysis of recombination in Saccharomyces
    J E Haber
    Brandeis University, Rosenstiel Center, Mailstop 029, Waltham, MA 02454 9110, USA
    Mutat Res 451:53-69. 2000
  24. ncbi request reprint Partners and pathwaysrepairing a double-strand break
    J E Haber
    Rosentiel Basic Medical Sciences Research Center, MS 029 Brandeis University, Waltham, MA 02454 9110, USA
    Trends Genet 16:259-64. 2000
    ..Many of these pathways compete actively for the repair of a double-strand break. Which of these repair pathways is used appears to be regulated developmentally, genetically and during the cell cycle...
  25. ncbi request reprint Saccharomyces Ku70, mre11/rad50 and RPA proteins regulate adaptation to G2/M arrest after DNA damage
    S E Lee
    Rosenstiel Center, Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Cell 94:399-409. 1998
    ..We suggest that escape from the DNA damage-induced G2/M checkpoint depends on the extent of ssDNA created at broken chromosome ends. RPA appears to play a key intermediate step in this adaptation...
  26. pmc Cell cycle and genetic requirements of two pathways of nonhomologous end-joining repair of double-strand breaks in Saccharomyces cerevisiae
    J K Moore
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02254 09110, USA
    Mol Cell Biol 16:2164-73. 1996
    ....
  27. pmc RAD1 and RAD10, but not other excision repair genes, are required for double-strand break-induced recombination in Saccharomyces cerevisiae
    E L Ivanov
    Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts 02254 9110
    Mol Cell Biol 15:2245-51. 1995
    ..Our results provide a molecular basis for understanding the role of yeast nucleotide excision repair gene and their human homologs in DSB-induced recombination and repair...
  28. pmc Efficient repair of HO-induced chromosomal breaks in Saccharomyces cerevisiae by recombination between flanking homologous sequences
    N Rudin
    Department of Biology, Brandeis University, Waltham, Massachusetts 02254
    Mol Cell Biol 8:3918-28. 1988
    ..Deletion-repair requires approximately 45 min and is inhibited when cycloheximide is added after HO endonuclease cleavage...
  29. ncbi request reprint Removal of nonhomologous DNA ends in double-strand break recombination: the role of the yeast ultraviolet repair gene RAD1
    J Fishman-Lobell
    Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02254
    Science 258:480-4. 1992
    ..These data suggest RAD1 is required to remove nonhomologous DNA from the 3' ends of recombining DNA, a process analogous to the excision of photodimers during repair of ultraviolet-damaged DNA...
  30. ncbi request reprint Double-strand break repair in yeast requires both leading and lagging strand DNA polymerases
    A M Holmes
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Cell 96:415-24. 1999
    ..Replication is terminated by capture of the second end of the DSB...
  31. ncbi request reprint MOP2 (SLA2) affects the abundance of the plasma membrane H(+)-ATPase of Saccharomyces cerevisiae
    S Na
    Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts 02254
    J Biol Chem 270:6815-23. 1995
    ..We conclude that MOP2 (SLA2) encodes a plasma membrane-associated protein that is required for the accumulation and/or maintenance of plasma membrane H(+)-ATPase on the cell surface...
  32. pmc Mcm1 regulates donor preference controlled by the recombination enhancer in Saccharomyces mating-type switching
    C Wu
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02254 9110 USA
    Genes Dev 12:1726-37. 1998
    ..Thus, Mcm1 is critically responsible for the activation as well as the Matalpha2-Mcm1-mediated repression of RE activity...
  33. ncbi request reprint The TSM1 gene of Saccharomyces cerevisiae overlaps the MAT locus
    B L Ray
    Rosenstiel Basic Medical Research Center, Brandeis University, Waltham, MA 02254
    Curr Genet 20:25-31. 1991
    ..Surprisingly, the TSM1 open reading frame of 1,410 amino acids extends into the MAT locus, such that the 3'-end of the MAT alpha 1 transcript ends 15 bp from the 3'-end of the TSM1 open reading frame...
  34. pmc Cycloheximide-resistant temperature-sensitive lethal mutations of Saccharomyces cerevisiae
    J H McCusker
    Department of Biology, Brandeis University, Waltham, Massachusetts 02254
    Genetics 119:303-15. 1988
    ..We also describe two new methods for the enrichment of auxotrophic mutations from a wild-type yeast strain...
  35. ncbi request reprint Surviving the breakup: the DNA damage checkpoint
    Jacob C Harrison
    Department of Biology and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts 02445, USA
    Annu Rev Genet 40:209-35. 2006
    ..Following DNA repair, the checkpoint pathway is inactivated in a process termed recovery. A related but genetically distinct process, adaptation, controls cell cycle re-entry in the face of unrepairable damage...
  36. ncbi request reprint Transpositions and translocations induced by site-specific double-strand breaks in budding yeast
    James E Haber
    MS029 Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02454 9110, USA
    DNA Repair (Amst) 5:998-1009. 2006
    ..These rearrangements can occur from ectopic gene conversions accompanied by crossing-over, break-induced replication, single-strand annealing or non-homologous end-joining...
  37. pmc Cell cycle-dependent regulation of Saccharomyces cerevisiae donor preference during mating-type switching by SBF (Swi4/Swi6) and Fkh1
    Eric Coïc
    Department of Biology and Rosenstiel Center, Brandeis University, Waltham, MA 02254 9110, USA
    Mol Cell Biol 26:5470-80. 2006
    ..In addition, the putative helicase Chl1, previously involved in donor preference, functions in the SBF pathway...
  38. ncbi request reprint Gene amplification: yeast takes a turn
    James E Haber
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02254, USA
    Cell 125:1237-40. 2006
    ..Recent studies in budding yeast, including in this issue of Cell, have provided new insights into the role of palindromic sequences in gene amplification...
  39. pmc Chromosome breakage and repair
    James E Haber
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Genetics 173:1181-5. 2006
  40. ncbi request reprint Repair of DNA double strand breaks: in vivo biochemistry
    Neal Sugawara
    Rosenstiel Center, Brandeis University, Waltham, Massachusetts, USA
    Methods Enzymol 408:416-29. 2006
    ....
  41. pmc Different mating-type-regulated genes affect the DNA repair defects of Saccharomyces RAD51, RAD52 and RAD55 mutants
    Maria Valencia-Burton
    Department of Biology and Resenstiel Center, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Genetics 174:41-55. 2006
    ..All three recombination-defective mutations are made more sensitive by deletions of Rad6 and of the histone deacetylases Rpd3 and Ume6, although these mutations are not themselves CPT or phleomycin sensitive...
  42. ncbi request reprint Break-induced replication and recombinational telomere elongation in yeast
    Michael J McEachern
    Department of Genetics, University of Georgia, Athens, Georgia 30602, USA
    Annu Rev Biochem 75:111-35. 2006
    ..Additional BIR events can then copy the elongated sequence to all other telomeres...
  43. pmc Saccharomyces cerevisiae donor preference during mating-type switching is dependent on chromosome architecture and organization
    Eric Coïc
    Department of Biology and Rosenstiel Center, Brandeis University, Waltham, Massachusetts 02254 9110, USA
    Genetics 173:1197-206. 2006
    ..We propose that RE's targets are anchor sites that tether chromosome III-L in MATalpha cells thus reducing its mobility in the nucleus...
  44. ncbi request reprint A phosphatase complex that dephosphorylates gammaH2AX regulates DNA damage checkpoint recovery
    Michael Christopher Keogh
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 439:497-501. 2006
    ..The dephosphorylation of gammaH2AX by the HTP-C is necessary for efficient recovery from the DNA damage checkpoint...
  45. pmc Inactivation of Ku-mediated end joining suppresses mec1Delta lethality by depleting the ribonucleotide reductase inhibitor Sml1 through a pathway controlled by Tel1 kinase and the Mre11 complex
    Yves Corda
    Laboratoire d Ingenierie des Systemes Macromoleculaires, IBSM, CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille, Cedex 20, France
    Mol Cell Biol 25:10652-64. 2005
    ..We further report that this Mec1-independent pathway converges with the Rad53/Dun1-regulated checkpoint kinase cascade and leads to the degradation of the ribonucleotide reductase inhibitor Sml1...
  46. ncbi request reprint The MRE11-RAD50-XRS2 complex, in addition to other non-homologous end-joining factors, is required for V(D)J joining in yeast
    Anne E Clatworthy
    Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    J Biol Chem 280:20247-52. 2005
    ..In addition, we showed an absolute requirement for the MRX complex in signal joining, suggesting that the Mre11-Rad50-Nbs1 complex may be required for signal joint formation in mammalian cells as well...
  47. ncbi request reprint Smc5-Smc6 mediate DNA double-strand-break repair by promoting sister-chromatid recombination
    Giacomo De Piccoli
    Cell Cycle Group, MRC Clinical Sciences Centre, Imperial College London, Du Cane Road, London W12 0NN, UK
    Nat Cell Biol 8:1032-4. 2006
    ..Our findings show that the Smc5-Smc6 complex is essential for genome stability as it promotes repair of DSBs by error-free sister-chromatid recombination (SCR), thereby suppressing inappropriate non-sister recombination events...
  48. ncbi request reprint Comment on "Cell type regulates selective segregation of mouse chromosome 7 DNA strands in mitosis"
    James E Haber
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02454 9110, USA
    Science 313:1045; author reply 1045. 2006
    ..However, this interpretation only considered half of the possible outcomes. The conjecture fails when all possible outcomes are examined...
  49. pmc Mre11-Rad50-Nbs1-dependent processing of DNA breaks generates oligonucleotides that stimulate ATM activity
    Ali Jazayeri
    Genome Stability Unit, Clare Hall Laboratories, London Research Institute, South Mimms, Herts, UK
    EMBO J 27:1953-62. 2008
    ..These results suggest that MRN-dependent generation of ssDNA oligos, which constitute a unique signal of ongoing DSB repair not encountered in normal DNA metabolism, stimulates ATM activity...
  50. pmc Mechanisms of Rad52-independent spontaneous and UV-induced mitotic recombination in Saccharomyces cerevisiae
    Eric Coïc
    Department of Biology and Rosenstiel Center, Brandeis University, Waltham, MA 02454 9110, USA
    Genetics 179:199-211. 2008
    ..Thus, there are at least two Rad52-independent recombination pathways in budding yeast...
  51. pmc Histone methyltransferase Dot1 and Rad9 inhibit single-stranded DNA accumulation at DSBs and uncapped telomeres
    Federico Lazzaro
    Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita degli Studi di Milano, Milano, Italy
    EMBO J 27:1502-12. 2008
    ....
  52. pmc Functional interactions between Sae2 and the Mre11 complex
    Hee Sook Kim
    Laboratory of Chromosome Biology, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Genetics 178:711-23. 2008
    ..We show that Sae2 oligomerizes independently of DNA damage and that oligomerization is required for its regulatory influence on the Mre11 nuclease and checkpoint functions...
  53. pmc Alternative endings
    James E Haber
    Department of Biology and Rosenstiel Center, Brandeis University, Waltham, MA 02454 9110, USA
    Proc Natl Acad Sci U S A 105:405-6. 2008
  54. ncbi request reprint Mec1/Tel1 phosphorylation of the INO80 chromatin remodeling complex influences DNA damage checkpoint responses
    Ashby J Morrison
    Department of Carcinogenesis, Science Park Research Division, University of Texas M D Anderson Cancer Center, Smithville, TX 78957, USA
    Cell 130:499-511. 2007
    ....
  55. ncbi request reprint Break-induced replication and telomerase-independent telomere maintenance require Pol32
    John R Lydeard
    MS029 Rosenstiel Centre, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Nature 448:820-3. 2007
    ..We also note that Pol32 homologues have been identified both in fission yeast and in metazoans where telomerase-independent survivors with alternative telomere maintenance have also been identified...
  56. pmc Phosphorylation of Slx4 by Mec1 and Tel1 regulates the single-strand annealing mode of DNA repair in budding yeast
    Sonja Flott
    MRC Protein Phosphorylation Unit, James Black Centre, University of Dundee, Dundee DD1 5EH, United Kingdom
    Mol Cell Biol 27:6433-45. 2007
    ..These results indicate that Slx4 has multiple functions in responding to DNA damage and that a subset of these are regulated by Mec1/Tel1-dependent phosphorylation...
  57. pmc Heterochromatin is refractory to gamma-H2AX modification in yeast and mammals
    Jung Ae Kim
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02454, USA
    J Cell Biol 178:209-18. 2007
    ..In yeast, we see a high level of constitutive gamma-H2AX in telomere regions in the absence of any exogenous DNA damage, suggesting that yeast chromosome ends are transiently detected as DSBs...
  58. pmc The yeast DNA damage checkpoint proteins control a cytoplasmic response to DNA damage
    Farokh Dotiwala
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02454 9110, USA
    Proc Natl Acad Sci U S A 104:11358-63. 2007
    ..We suggest that Mec1-dependent checkpoint signaling through Rad53 and Chk1 includes the repression of nuclear movements that are normally associated with the execution of anaphase...
  59. ncbi request reprint Anaphase onset before complete DNA replication with intact checkpoint responses
    Jordi Torres-Rosell
    Cell Cycle Group, Medical Research Council MRC Clinical Sciences Centre, Faculty of Medicine, Imperial College London, Hammersmith Hospital Campus, Du Cane Road, London W12 0NN, UK
    Science 315:1411-5. 2007
    ..We propose that the completion of replication is not under the surveillance of known checkpoints...
  60. pmc Conservative inheritance of newly synthesized DNA in double-strand break-induced gene conversion
    Grzegorz Ira
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02453 2728, USA
    Mol Cell Biol 26:9424-9. 2006
    ....
  61. pmc Rad51-dependent DNA structures accumulate at damaged replication forks in sgs1 mutants defective in the yeast ortholog of BLM RecQ helicase
    Giordano Liberi
    F I R C Institute of Molecular Oncology Foundation, 20141, Milan, Italy
    Genes Dev 19:339-50. 2005
    ..Our findings might contribute to explaining some of the recombination defects of BLM cells...
  62. pmc RAD51-dependent break-induced replication differs in kinetics and checkpoint responses from RAD51-mediated gene conversion
    Anna Malkova
    Rosenstiel Center, Brandeis University, 415 South St, Mail Stop 029, Waltham, MA 02454 9110, USA
    Mol Cell Biol 25:933-44. 2005
    ..Once repair is initiated, the rate of repair replication during BIR is comparable to that of normal DNA replication, as copying of >100 kb is completed less than 30 min after repair DNA synthesis is detected close to the DSB...
  63. pmc Microhomology-dependent end joining and repair of transposon-induced DNA hairpins by host factors in Saccharomyces cerevisiae
    Jianhua Yu
    Department of Agronomy, Purdue University, West Lafayette, Indiana 47907 1150, USA
    Mol Cell Biol 24:1351-64. 2004
    ..In addition, the interstrand cross-link repair gene PSO2 plays a role in end joining hairpin ends that is not seen in repair of linearized plasmids and may be involved in positioning transposase cleavage at the transposon ends...
  64. ncbi request reprint Srs2 and Sgs1-Top3 suppress crossovers during double-strand break repair in yeast
    Grzegorz Ira
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02454, USA
    Cell 115:401-11. 2003
    ..Srs2 promotes the noncrossover synthesis-dependent strand-annealing (SDSA) pathway, apparently by regulating Rad51 binding during strand exchange...
  65. pmc Yeast Rad52 and Rad51 recombination proteins define a second pathway of DNA damage assessment in response to a single double-strand break
    Sang Eun Lee
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Mol Cell Biol 23:8913-23. 2003
    ..We suggest that monitoring of the extent of DNA damage depends on independent binding of RPA and Rad52p to ssDNA, with Rad52p's activity modulated by Rad51p whereas RPA's action depends on Tid1p...
  66. pmc Yeast Mre11 and Rad1 proteins define a Ku-independent mechanism to repair double-strand breaks lacking overlapping end sequences
    Jia Lin Ma
    Department of Molecular Medicine, Institute of Biotechnology, The University of Texas Health Science Center at San Antonio, San Antonio, Texas 78245, USA
    Mol Cell Biol 23:8820-8. 2003
    ..The increased gamma ray sensitivity of rad1Delta rad52Delta yku70Delta strains compared to rad52Delta yku70Delta strains suggests that MMEJ also contributes to the repair of DSBs induced by ionizing radiation...
  67. ncbi request reprint Aging: the sins of the parents
    James E Haber
    Rosenstiel Center MS029, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Curr Biol 13:R843-5. 2003
    ....
  68. ncbi request reprint V(D)J recombination and RAG-mediated transposition in yeast
    Anne E Clatworthy
    Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA
    Mol Cell 12:489-99. 2003
    ..Cleaved signal ends are also transposed into new sites in DNA, allowing RAG-induced transposition to be studied in vivo...
  69. ncbi request reprint In vivo roles of Rad52, Rad54, and Rad55 proteins in Rad51-mediated recombination
    Neal Sugawara
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02454, USA
    Mol Cell 12:209-19. 2003
    ....
  70. ncbi request reprint PP2C phosphatases Ptc2 and Ptc3 are required for DNA checkpoint inactivation after a double-strand break
    Christophe Leroy
    Service de Biochimie et de Génétique Moléculaire, CEA Saclay, 91191 Gif sur Yvette, Cedex, France
    Mol Cell 11:827-35. 2003
    ..In vivo and in vitro evidence suggests that phosphorylated forms of Ptc2 and Ptc3 specifically bind to the Rad53 FHA1 domain and inactivate Rad53-dependent pathways during adaptation and recovery by dephosphorylating Rad53...
  71. ncbi request reprint Complementation between N-terminal Saccharomyces cerevisiae mre11 alleles in DNA repair and telomere length maintenance
    Sang Eun Lee
    Rosenstiel Center and Department of Biology, Brandeis University, MS029 Waltham, MA 02454 9110, USA
    DNA Repair (Amst) 1:27-40. 2002
    ..We propose that at least two separate activities associated with the N-terminus of Mre11p are required for its mitotic function...
  72. pmc Characterization of RAD51-independent break-induced replication that acts preferentially with short homologous sequences
    Grzegorz Ira
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachussetts 02454 9110, USA
    Mol Cell Biol 22:6384-92. 2002
    ..The differences between the RAD51- and RAD50/RAD59-dependent pathways account for the distinct ways that two different recombination processes maintain yeast telomeres in the absence of telomerase...
  73. ncbi request reprint Recovery from checkpoint-mediated arrest after repair of a double-strand break requires Srs2 helicase
    Moreshwar B Vaze
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02454, USA
    Mol Cell 10:373-85. 2002
    ..Permanent preanaphase arrest of srs2Delta cells is reversed by the addition of caffeine after cells have arrested. Thus, in addition to its roles in recombination, Srs2p appears to be needed to turn off the DNA damage checkpoint...
  74. pmc Saccharomyces forkhead protein Fkh1 regulates donor preference during mating-type switching through the recombination enhancer
    Kaiming Sun
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02254 9910, USA
    Genes Dev 16:2085-96. 2002
    ..Deletion of FKH1 significantly reduces MATa's use of HML, as does mutation of the Fkh1/Fkh2-binding sites in a multimer of region A. We conclude that Fkh1p regulates MATa donor preference through direct interaction with RE...
  75. pmc Role of Saccharomyces single-stranded DNA-binding protein RPA in the strand invasion step of double-strand break repair
    Xuan Wang
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts, USA
    PLoS Biol 2:E21. 2004
    ..Thus, RPA appears to play a role in strand invasion as well as in facilitating Rad51 binding to ssDNA, possibly by stabilizing the displaced ssDNA...
  76. pmc Mating type-dependent constraints on the mobility of the left arm of yeast chromosome III
    Debra A Bressan
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02454 9910, USA
    J Cell Biol 164:361-71. 2004
    ..These data suggest there is constitutive tethering of HML, which is relieved in MATa cells through the action of RE...
  77. ncbi request reprint DNA damage response pathway uses histone modification to assemble a double-strand break-specific cohesin domain
    Elcin Unal
    Howard Hughes Medical Institute, Department of Embryology, The Carnegie Institution of Washington, Baltimore, MD 21210, USA
    Mol Cell 16:991-1002. 2004
    ..We also provide evidence that the DSB-induced cohesin domain functions in postreplicative repair...
  78. ncbi request reprint INO80 and gamma-H2AX interaction links ATP-dependent chromatin remodeling to DNA damage repair
    Ashby J Morrison
    Department of Carcinogenesis, MD Anderson Cancer Center, Science Park Research Division, Smithville, TX 78957, USA
    Cell 119:767-75. 2004
    ..Our findings reveal a new role of ATP-dependent chromatin remodeling in nuclear processes and suggest that an ATP-dependent chromatin remodeling complex can read a DNA repair histone code...
  79. ncbi request reprint DNA breaks promote genomic instability by impeding proper chromosome segregation
    Julia A Kaye
    Cancer Research Institute, Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94115, USA
    Curr Biol 14:2096-106. 2004
    ..Here, we have examined the machinery that holds broken chromosome ends together, and we have explored the behavior of broken chromosomes as they pass through mitosis...
  80. ncbi request reprint Telomeres thrown for a loop
    James E Haber
    Brandeis University, Waltham, Massachusetts 02454, USA
    Mol Cell 16:502-3. 2004
    ....
  81. ncbi request reprint DNA end resection, homologous recombination and DNA damage checkpoint activation require CDK1
    Grzegorz Ira
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Nature 431:1011-7. 2004
    ..CDK1 is also important for a later step in homologous recombination, after strand invasion and before the initiation of new DNA synthesis...
  82. ncbi request reprint Distribution and dynamics of chromatin modification induced by a defined DNA double-strand break
    Robert Shroff
    Laboratory of Biochemistry, Center for Cancer Research, National Cancer Institute, Building 37, Room 6124, Bethesda, MD 20892, USA
    Curr Biol 14:1703-11. 2004
    ..To date, these events have been defined primarily at the resolution of light microscopes, and the relationship between gamma-H2AX formation and repair protein recruitment remains to be defined...
  83. pmc Gene conversion and crossing over along the 405-kb left arm of Saccharomyces cerevisiae chromosome VII
    Anna Malkova
    Department of Biology and Rosenstiel Center, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    Genetics 168:49-63. 2004
    ....
  84. pmc Role of DNA replication proteins in double-strand break-induced recombination in Saccharomyces cerevisiae
    Xuan Wang
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02454 9110, USA
    Mol Cell Biol 24:6891-9. 2004
    ..We conclude that DNA synthesis during gene conversion is different from S-phase replication, involving only leading-strand polymerization...
  85. pmc Heteroduplex rejection during single-strand annealing requires Sgs1 helicase and mismatch repair proteins Msh2 and Msh6 but not Pms1
    Neal Sugawara
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02454 9110, USA
    Proc Natl Acad Sci U S A 101:9315-20. 2004
    ..This conclusion is supported by the finding that deleting the SGS1 helicase also suppressed heteroduplex rejection...
  86. pmc Repairing a double-strand chromosome break by homologous recombination: revisiting Robin Holliday's model
    James E Haber
    Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02454 9110, USA
    Philos Trans R Soc Lond B Biol Sci 359:79-86. 2004
    ....
  87. pmc In vivo assembly and disassembly of Rad51 and Rad52 complexes during double-strand break repair
    Toshiko Miyazaki
    Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka, Japan
    EMBO J 23:939-49. 2004
    ..Additional biochemical studies suggest the presence of an ssDNA complex containing both Rad51 and Rad52...
  88. ncbi request reprint Uses and abuses of HO endonuclease
    James E Haber
    Rosenstiel Center, Department of Biology, Brandeis University, Waltham, Massachusetts 02454, USA
    Methods Enzymol 350:141-64. 2002
  89. ncbi request reprint SMC proteins, new players in the maintenance of genomic stability
    Felipe Cortes-Ledesma
    Department of Molecular Biology, CABIMER, CSIC Universidad de Sevilla, Sevilla, Spain
    Cell Cycle 6:914-8. 2007
    ..A deeper knowledge of the role of SMC proteins in DSB repair should contribute to a better understanding of chromosome dynamics and stability...
  90. pmc The 2005 Genetics Society of America Medal. Steven J. Elledge
    James E Haber
    Genetics 169:506-7. 2005