Vassilis Zannis

Summary

Affiliation: Boston University
Country: USA

Publications

  1. ncbi request reprint Transcriptional regulation of the human apolipoprotein genes
    V I Zannis
    Section of Molecular Genetics, Whitaker Cardiovascular Institute, Departments of Medicine and Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118 239, USA
    Front Biosci 6:D456-504. 2001
  2. ncbi request reprint Transcriptional regulatory mechanisms of the human apolipoprotein genes in vitro and in vivo
    V I Zannis
    Section of Molecular Genetics, Whitaker Cardiovascular Institute, Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118 2394, USA
    Curr Opin Lipidol 12:181-207. 2001
  3. ncbi request reprint Role of apoA-I, ABCA1, LCAT, and SR-BI in the biogenesis of HDL
    Vassilis I Zannis
    Molecular Genetics, Whitaker Cardiovascular Institute and Department of Biochemistry, Boston University School of Medicine, MA 02118, USA
    J Mol Med (Berl) 84:276-94. 2006
  4. ncbi request reprint Regulatory gene mutations affecting apolipoprotein gene expression: functions and regulatory behavior of known genes may guide future pharmacogenomic approaches to therapy
    Vassilis I Zannis
    Section of Molecular Genetics, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA 02118, USA
    Clin Chem Lab Med 41:411-24. 2003
  5. doi request reprint Discrete roles of apoA-I and apoE in the biogenesis of HDL species: lessons learned from gene transfer studies in different mouse models
    Vassilis I Zannis
    Molecular Genetics, Whitaker Cardiovascular Institute, Departments of Medicine and Biochemistry, Boston University School of Medicine, Boston, MA 02118 2394, USA
    Ann Med 40:14-28. 2008
  6. ncbi request reprint Substitutions of glutamate 110 and 111 in the middle helix 4 of human apolipoprotein A-I (apoA-I) by alanine affect the structure and in vitro functions of apoA-I and induce severe hypertriglyceridemia in apoA-I-deficient mice
    Angeliki Chroni
    Molecular Genetics, Whitaker Cardiovascular Institute, Department of Medicine, Boston University School of Medicine, Massachusetts, USA
    Biochemistry 43:10442-57. 2004
  7. ncbi request reprint The central helices of ApoA-I can promote ATP-binding cassette transporter A1 (ABCA1)-mediated lipid efflux. Amino acid residues 220-231 of the wild-type ApoA-I are required for lipid efflux in vitro and high density lipoprotein formation in vivo
    Angeliki Chroni
    Section of Molecular Genetics, Whitaker Cardiovascular Institute, Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Biol Chem 278:6719-30. 2003
  8. pmc Pathway of biogenesis of apolipoprotein E-containing HDL in vivo with the participation of ABCA1 and LCAT
    Kyriakos E Kypreos
    Molecular Genetics, Whitaker Cardiovascular Institute, Departments of Medicine and Biochemistry, Boston University School of Medicine, 715 Albany Street W509, Boston, MA 02118, USA
    Biochem J 403:359-67. 2007
  9. ncbi request reprint Lipid-free structure and stability of apolipoprotein A-I: probing the central region by mutation
    Irina N Gorshkova
    Department of Physiology and Biophysics and Section of Molecular Genetics, Whitaker Cardiovascular Institute, Boston University School of Medicine, 715 Albany Street, Boston, Massachusetts 02118, USA
    Biochemistry 41:10529-39. 2002
  10. pmc Naturally occurring and bioengineered apoA-I mutations that inhibit the conversion of discoidal to spherical HDL: the abnormal HDL phenotypes can be corrected by treatment with LCAT
    Georgios Koukos
    Molecular Genetics, Departmental of Medicine and Biochemistry, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA 02118, USA
    Biochem J 406:167-74. 2007

Research Grants

  1. Functions of apoE in cholesterol and triglyceride homeostasis
    Vassilis I Zannis; Fiscal Year: 2010
  2. APOLIPOPROTEIN VARIATION AND HUMAN DISEASE
    Vassilis Zannis; Fiscal Year: 2003
  3. ANALYSIS OF HUMAN APOLIPOPROTEIN A-I
    Vassilis Zannis; Fiscal Year: 2002
  4. ApoE in Cholesterol and Triglyceride Homeostasis
    Vassilis Zannis; Fiscal Year: 2004
  5. Molecular and Funcitonal Analysis of Human A-I
    Vassilis I Zannis; Fiscal Year: 2010
  6. Functions of apoE in cholesterol and triglyceride homeostasis
    Vassilis Zannis; Fiscal Year: 2007
  7. Molecular and Functional Analysis of Human ApoA-1
    Vassilis Zannis; Fiscal Year: 2006
  8. APOLIPOPROTEIN VARIATION AND HUMAN DISEASE
    Vassilis Zannis; Fiscal Year: 1999
  9. APOLIPOPROTEIN VARIATION AND HUMAN DISEASE
    Vassilis Zannis; Fiscal Year: 1993
  10. APOPROTEIN VARIATION AND HUMAN DISEASE
    Vassilis Zannis; Fiscal Year: 1990

Collaborators

Detail Information

Publications42

  1. ncbi request reprint Transcriptional regulation of the human apolipoprotein genes
    V I Zannis
    Section of Molecular Genetics, Whitaker Cardiovascular Institute, Departments of Medicine and Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118 239, USA
    Front Biosci 6:D456-504. 2001
    ..Understanding the transcriptional regulatory mechanism of the apolipoprotein genes may allow, in the long run, selective increase of anti-atherogenic lipoproteins and thus reduce the risk of cardiovascular disease...
  2. ncbi request reprint Transcriptional regulatory mechanisms of the human apolipoprotein genes in vitro and in vivo
    V I Zannis
    Section of Molecular Genetics, Whitaker Cardiovascular Institute, Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118 2394, USA
    Curr Opin Lipidol 12:181-207. 2001
    ..This may have beneficial effects and may confer protection from atherosclerosis to humans...
  3. ncbi request reprint Role of apoA-I, ABCA1, LCAT, and SR-BI in the biogenesis of HDL
    Vassilis I Zannis
    Molecular Genetics, Whitaker Cardiovascular Institute and Department of Biochemistry, Boston University School of Medicine, MA 02118, USA
    J Mol Med (Berl) 84:276-94. 2006
    ....
  4. ncbi request reprint Regulatory gene mutations affecting apolipoprotein gene expression: functions and regulatory behavior of known genes may guide future pharmacogenomic approaches to therapy
    Vassilis I Zannis
    Section of Molecular Genetics, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA 02118, USA
    Clin Chem Lab Med 41:411-24. 2003
    ....
  5. doi request reprint Discrete roles of apoA-I and apoE in the biogenesis of HDL species: lessons learned from gene transfer studies in different mouse models
    Vassilis I Zannis
    Molecular Genetics, Whitaker Cardiovascular Institute, Departments of Medicine and Biochemistry, Boston University School of Medicine, Boston, MA 02118 2394, USA
    Ann Med 40:14-28. 2008
    ..The apoE-containing HDL particles formed in the circulation may have atheroprotective properties. ApoE-containing HDL may also have important biological functions in the brain that confer protection from Alzheimer's disease...
  6. ncbi request reprint Substitutions of glutamate 110 and 111 in the middle helix 4 of human apolipoprotein A-I (apoA-I) by alanine affect the structure and in vitro functions of apoA-I and induce severe hypertriglyceridemia in apoA-I-deficient mice
    Angeliki Chroni
    Molecular Genetics, Whitaker Cardiovascular Institute, Department of Medicine, Boston University School of Medicine, Massachusetts, USA
    Biochemistry 43:10442-57. 2004
    ..The findings indicate that subtle structural alterations in apoA-I may alter the stability and functions of apoA-I and high-density lipoprotein (HDL) and may cause hypertriglyceridemia...
  7. ncbi request reprint The central helices of ApoA-I can promote ATP-binding cassette transporter A1 (ABCA1)-mediated lipid efflux. Amino acid residues 220-231 of the wild-type ApoA-I are required for lipid efflux in vitro and high density lipoprotein formation in vivo
    Angeliki Chroni
    Section of Molecular Genetics, Whitaker Cardiovascular Institute, Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Biol Chem 278:6719-30. 2003
    ....
  8. pmc Pathway of biogenesis of apolipoprotein E-containing HDL in vivo with the participation of ABCA1 and LCAT
    Kyriakos E Kypreos
    Molecular Genetics, Whitaker Cardiovascular Institute, Departments of Medicine and Biochemistry, Boston University School of Medicine, 715 Albany Street W509, Boston, MA 02118, USA
    Biochem J 403:359-67. 2007
    ..HDL particles generated by this pathway may account at least for some of the atheroprotective functions of apoE...
  9. ncbi request reprint Lipid-free structure and stability of apolipoprotein A-I: probing the central region by mutation
    Irina N Gorshkova
    Department of Physiology and Biophysics and Section of Molecular Genetics, Whitaker Cardiovascular Institute, Boston University School of Medicine, 715 Albany Street, Boston, Massachusetts 02118, USA
    Biochemistry 41:10529-39. 2002
    ....
  10. pmc Naturally occurring and bioengineered apoA-I mutations that inhibit the conversion of discoidal to spherical HDL: the abnormal HDL phenotypes can be corrected by treatment with LCAT
    Georgios Koukos
    Molecular Genetics, Departmental of Medicine and Biochemistry, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA 02118, USA
    Biochem J 406:167-74. 2007
    ..The correction of the aberrant HDL phenotypes by treatment with LCAT suggests a potential therapeutic intervention for HDL abnormalities that result from specific mutations in apoA-I...
  11. ncbi request reprint Domains of apoE required for binding to apoE receptor 2 and to phospholipids: implications for the functions of apoE in the brain
    Xiaoping Li
    Whitaker Cardiovascular Institute, Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    Biochemistry 42:10406-17. 2003
    ..3 microg/mL). The findings establish that the apoER2 binding domain of apoE is in the 1-165 amino terminal region, whereas the carboxy terminal 230-299 region of apoE is required for efficient initial association with phospholipids...
  12. ncbi request reprint Deletions of helices 2 and 3 of human apoA-I are associated with severe dyslipidemia following adenovirus-mediated gene transfer in apoA-I-deficient mice
    Angeliki Chroni
    Section of Molecular Genetics, Whitaker Cardiovascular Institute, Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    Biochemistry 44:4108-17. 2005
    ..We conclude that alterations in apoA-I not only may have adverse effects on HDL biosynthesis but also may promote dyslipidemia due to interference of the apoA-I mutants on the overall cholesterol and triglycerides homeostasis...
  13. pmc Residues Leu261, Trp264, and Phe265 account for apolipoprotein E-induced dyslipidemia and affect the formation of apolipoprotein E-containing high-density lipoprotein
    Konstantinos Drosatos
    Molecular Genetics, Department of Medicine, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    Biochemistry 46:9645-53. 2007
    ..Substitution of these residues with Ala improves the apoE functions by preventing hypertriglyceridemia and promoting formation of spherical apoE-containing HDL...
  14. ncbi request reprint Generation of a recombinant apolipoprotein E variant with improved biological functions: hydrophobic residues (LEU-261, TRP-264, PHE-265, LEU-268, VAL-269) of apoE can account for the apoE-induced hypertriglyceridemia
    Kyriakos E Kypreos
    Molecular Genetics, Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Biol Chem 280:6276-84. 2005
    ..Substitutions of these residues by alanine provide a recombinant apoE form with improved biological functions...
  15. ncbi request reprint LCAT can rescue the abnormal phenotype produced by the natural ApoA-I mutations (Leu141Arg)Pisa and (Leu159Arg)FIN
    Georgios Koukos
    Molecular Genetics, Department of Medicine, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    Biochemistry 46:10713-21. 2007
    ..Both defects can be corrected by treatment with LCAT...
  16. ncbi request reprint Reconstituted discoidal ApoE-phospholipid particles are ligands for the scavenger receptor BI. The amino-terminal 1-165 domain of ApoE suffices for receptor binding
    Xiaoping Li
    Section of Molecular Genetics, Whitaker Cardiovascular Institute, Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Biol Chem 277:21149-57. 2002
    ..SR-BI-apoE interactions may contribute to cholesterol homeostasis in tissues and cells expressing SR-BI that are accessible to apoE-containing lipoproteins...
  17. ncbi request reprint Probing the pathways of chylomicron and HDL metabolism using adenovirus-mediated gene transfer
    Vassilis I Zannis
    Molecular Genetics, Boston University School of Medicine, Boston, Massachusetts 02118 2394, USA vzannis bu edu
    Curr Opin Lipidol 15:151-66. 2004
    ..This review clarifies the functions of key proteins of the chylomicron and the HDL pathways...
  18. ncbi request reprint LDL receptor deficiency or apoE mutations prevent remnant clearance and induce hypertriglyceridemia in mice
    Kyriakos E Kypreos
    Molecular Genetics, Departments of Medicine and Biochemistry, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA 02118, USA
    J Lipid Res 47:521-9. 2006
    ....
  19. ncbi request reprint Cross-linking and lipid efflux properties of apoA-I mutants suggest direct association between apoA-I helices and ABCA1
    Angeliki Chroni
    Molecular Genetics, Whitaker Cardiovascular Institute, Department of Medicine, Boston University School of Medicine, 715 Albany Street W509, Boston, Massachusetts 02118, USA
    Biochemistry 43:2126-39. 2004
    ..The findings are consistent with a direct association of different combinations of apoA-I helices with a complementary ABCA1 domain. Mutations that alter ABCA1/apoA-I association affect cholesterol efflux and inhibit biogenesis of HDL...
  20. pmc Biophysical properties of apolipoprotein E4 variants: implications in molecular mechanisms of correction of hypertriglyceridemia
    Irina N Gorshkova
    Department of Physiology and Biophysics, Boston University School of Medicine, 700 Albany Street, Boston, Massachusetts 02118, USA
    Biochemistry 47:12644-54. 2008
    ..The slightly altered structural properties of apoE4-mut2 are associated with slightly reduced binding of this protein to TG-rich lipoprotein particles and milder hypertriglyceridemia as compared with WT apoE4...
  21. ncbi request reprint The effects of mutations in helices 4 and 6 of ApoA-I on scavenger receptor class B type I (SR-BI)-mediated cholesterol efflux suggest that formation of a productive complex between reconstituted high density lipoprotein and SR-BI is required for efficien
    Tong Liu
    Section of Molecular Genetics, Whitaker Cardiovascular Institute, Department of Medicine and Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Biol Chem 277:21576-84. 2002
    ..Some mutations in apoA-I and/or SR-BI can result in high affinity, but non-productive, binding that does not permit efficient cholesterol efflux...
  22. pmc Structure and stability of apolipoprotein a-I in solution and in discoidal high-density lipoprotein probed by double charge ablation and deletion mutation
    Irina N Gorshkova
    Department of Physiology and Biophysics, Boston University School of Medicine, 715 Albany Street, Boston, Massachusetts 02118, USA
    Biochemistry 45:1242-54. 2006
    ..The effects of alterations in the conformation and stability of the apoA-I mutants on in vitro and in vivo functions of apoA-I and lipid homeostasis are discussed...
  23. ncbi request reprint Molecular mechanisms of type III hyperlipoproteinemia: The contribution of the carboxy-terminal domain of ApoE can account for the dyslipidemia that is associated with the E2/E2 phenotype
    Kyriakos E Kypreos
    Molecular Genetics, Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118 2394, USA
    Biochemistry 42:9841-53. 2003
    ..In contrast, apoE2 exacerbated the hypercholesterolemia and induced hypertriglyceridemia, suggesting that the LDL receptor is the predominant receptor in remnant clearance...
  24. pmc A2b adenosine receptor regulates hyperlipidemia and atherosclerosis
    Milka Koupenova
    Department of Medicine, Boston University School of Medicine, 700 Albany St, CVI, W 601, Boston, MA 02118, USA
    Circulation 125:354-63. 2012
    ..The cAMP-elevating A(2b) adenosine receptor (A(2b)AR) controls inflammation via its expression in bone marrow cells...
  25. ncbi request reprint SR-BI mediates cholesterol efflux via its interactions with lipid-bound ApoE. Structural mutations in SR-BI diminish cholesterol efflux
    Angeliki Chroni
    Molecular Genetics, Whitaker Cardiovascular Institute, Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    Biochemistry 44:13132-43. 2005
    ..The SR-BI-apoE interactions may contribute to overall cholesterol homeostasis in cells and tissues that express SR-BI and apoE...
  26. ncbi request reprint Generation and characterization of two transgenic mouse lines expressing human ApoE2 in neurons and glial cells
    Spiros Georgopoulos
    Section of Molecular Genetics, Whitaker Cardiovascular Institute, Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    Biochemistry 41:9293-301. 2002
    ....
  27. ncbi request reprint Contribution of the hormone-response elements of the proximal ApoA-I promoter, ApoCIII enhancer, and C/EBP binding site of the proximal ApoA-I promoter to the hepatic and intestinal expression of the ApoA-I and ApoCIII genes in transgenic mice
    Horng Yuan Kan
    Section of Molecular Genetics, Center for Advanced Biomedical Research, Boston University School of Medicine, 715 Albany Street, Boston, Massachusetts 02118 2394, USA
    Biochemistry 43:5084-93. 2004
    ..These findings establish the importance of the HREs for the hepatic and intestinal expressions of the apoA-I and apoCIII genes and suggest that C/EBP does not play a central role in the expression of the apoA-I gene...
  28. ncbi request reprint Point mutations in apolipoprotein A-I mimic the phenotype observed in patients with classical lecithin:cholesterol acyltransferase deficiency
    Angeliki Chroni
    Molecular Genetics, Whitaker Cardiovascular Institute, Department of Medicine, Center for Advanced Biomedical Research, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    Biochemistry 44:14353-66. 2005
    ..The findings indicate a critical contribution of residue 160 of apoA-I to the in vivo activity of LCAT and the subsequent maturation of HDL and explain the low HDL levels in heterozygous subjects carrying this mutation...
  29. pmc MicroRNA-370 controls the expression of microRNA-122 and Cpt1alpha and affects lipid metabolism
    Dimitrios Iliopoulos
    Department of Biological Chemistry and Molecular Pharmacology, School of Medicine, Harvard University, Boston, MA, USA
    J Lipid Res 51:1513-23. 2010
    ....
  30. ncbi request reprint Hyperlipidemia in APOE2 transgenic mice is ameliorated by a truncated apoE variant lacking the C-terminal domain
    Gery Gerritsen
    Department of Human Genetics, Leiden University Medical Center, The Netherlands
    J Lipid Res 44:408-14. 2003
    ..Thus, apoE4-202 is a safe and efficient candidate for future therapeutic applications...
  31. ncbi request reprint Physical and functional interactions between liver X receptor/retinoid X receptor and Sp1 modulate the transcriptional induction of the human ATP binding cassette transporter A1 gene by oxysterols and retinoids
    Efstathia Thymiakou
    Laboratory of Biochemistry, Department of Basic Sciences, University of Crete Medical School, and Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology of Hellas, Heraklion, Crete 71110, Greece
    Biochemistry 46:11473-83. 2007
    ..Overall, the present study revealed a novel mechanism of regulation of the human ABCA1 transporter which involves synergistic interactions between oxysterol/retinoid-inducible hormone nuclear receptors and the transcription factor Sp1...
  32. pmc Synergism between nuclear receptors bound to specific hormone response elements of the hepatic control region-1 and the proximal apolipoprotein C-II promoter mediate apolipoprotein C-II gene regulation by bile acids and retinoids
    Dimitris Kardassis
    Department of Basic Sciences, School of Health Sciences, Faculty of Medicine, University of Crete, PO Box 1393, 71500 Heraklion, Greece
    Biochem J 372:291-304. 2003
    ....
  33. pmc Mechanism of a transcriptional cross talk between transforming growth factor-beta-regulated Smad3 and Smad4 proteins and orphan nuclear receptor hepatocyte nuclear factor-4
    Wan Chih Chou
    Department of Basic Sciences, University of Crete Medical School and Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology of Hellas, Heraklion GR 71110, Greece
    Mol Biol Cell 14:1279-94. 2003
    ..Furthermore, the specificity of this interaction for certain target promoters may play an important role in various hepatocyte functions, which are regulated by TGFbeta and the Smads...
  34. ncbi request reprint Inflammatory signaling pathways regulating ApoE gene expression in macrophages
    Anca V Gafencu
    Institute of Cellular Biology and Pathology Nicolae Simionescu, Bucharest, Romania
    J Biol Chem 282:21776-85. 2007
    ....
  35. ncbi request reprint Cross-inhibition of SR-BI- and ABCA1-mediated cholesterol transport by the small molecules BLT-4 and glyburide
    Thomas J F Nieland
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    J Lipid Res 45:1256-65. 2004
    ..The reciprocal inhibition of SR-BI and ABCA1 by BLT-4 and glyburide raises the possibility that these proteins may share similar or common steps in their mechanisms of lipid transport...
  36. pmc A dominant negative form of the transcription factor c-Jun affects genes that have opposing effects on lipid homeostasis in mice
    Konstantinos Drosatos
    Department of Basic Sciences, University of Crete Medical School, Heraklion GR 71110, Greece
    J Biol Chem 282:19556-64. 2007
    ....
  37. pmc The carboxy-terminal region of apoA-I is required for the ABCA1-dependent formation of alpha-HDL but not prebeta-HDL particles in vivo
    Angeliki Chroni
    Institute of Biology, National Center for Scientific Research Demokritos, Agia Paraskevi, Athens 15310, Greece
    Biochemistry 46:5697-708. 2007
    ....
  38. pmc Inhibition of hepatocyte nuclear factor 4 transcriptional activity by the nuclear factor kappaB pathway
    Varvara Nikolaidou-Neokosmidou
    Department of Basic Sciences, University of Crete Medical School and Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology Hellas, Heraklion 71003, Crete, Greece
    Biochem J 398:439-50. 2006
    ....
  39. ncbi request reprint ApoC-III deficiency prevents hyperlipidemia induced by apoE overexpression
    Gery Gerritsen
    Department of Human Genetics, Leiden University Medical Center, Leiden, The Netherlands
    J Lipid Res 46:1466-73. 2005
    ..Thus, Apoc3 deficiency can prevent apoE-induced hyperlipidemia associated with a 10-fold increased hepatic VLDL-TG production rate, most likely by alleviating the apoE-induced inhibition of VLDL-TG hydrolysis...
  40. pmc Identification of the molecular target of small molecule inhibitors of HDL receptor SR-BI activity
    Thomas J F Nieland
    Department of Biology, Massachusetts Institute of Technology, Room 68 483, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Biochemistry 47:460-72. 2008
    ..Consequently, BLTs may help determine the therapeutic potential of SR-BI-targeted pharmaceutical drugs...
  41. ncbi request reprint Specific mutations in ABCA1 have discrete effects on ABCA1 function and lipid phenotypes both in vivo and in vitro
    Roshni R Singaraja
    Center for Molecular Medicine and Therapeutics, University of British Columbia and Child and Family Research Institute, Vancouver, British Columbia, Canada
    Circ Res 99:389-97. 2006
    ....
  42. pmc Biophysical analysis of progressive C-terminal truncations of human apolipoprotein E4: insights into secondary structure and unfolding properties
    Angeliki Chroni
    Institute of Biology, National Centre for Scientific Research Demokritos, Aghia Paraskevi, Athens 15310, Greece
    Biochemistry 47:9071-80. 2008
    ....

Research Grants42

  1. Functions of apoE in cholesterol and triglyceride homeostasis
    Vassilis I Zannis; Fiscal Year: 2010
    ..Gene transfer in single or double knockout mice for apoA-l, apoE, SR-BI and biochemical analyses will be employed in these studies. ..
  2. APOLIPOPROTEIN VARIATION AND HUMAN DISEASE
    Vassilis Zannis; Fiscal Year: 2003
    ..Thus the information obtained from this project may provide rational approaches towards correcting low plasma HDL levels and reducing hypertriglyceridemia in humans. ..
  3. ANALYSIS OF HUMAN APOLIPOPROTEIN A-I
    Vassilis Zannis; Fiscal Year: 2002
    ..Understanding the biological functions of apoA-I and HDL which are relevant to the development of CAD may lead to new pharmacological approaches to prevent and/or treat these conditions. ..
  4. ApoE in Cholesterol and Triglyceride Homeostasis
    Vassilis Zannis; Fiscal Year: 2004
    ..We expect that apoE forms that can clear cholesterol and triglycerides and protect from atherosclerosis may provide new therapeutic tools in the near future for the correction of remnant removal disorders. ..
  5. Molecular and Funcitonal Analysis of Human A-I
    Vassilis I Zannis; Fiscal Year: 2010
    ..This information is important in the design of new pharmaceuticals to increase HDL levels while preserving the atheroprotective functions of HDL and thus decrease the risk for cardiovascular disease. ..
  6. Functions of apoE in cholesterol and triglyceride homeostasis
    Vassilis Zannis; Fiscal Year: 2007
    ..Gene transfer in single or double knockout mice for apoA-l, apoE, SR-BI and biochemical analyses will be employed in these studies. ..
  7. Molecular and Functional Analysis of Human ApoA-1
    Vassilis Zannis; Fiscal Year: 2006
    ..Understanding the molecular structure and the various biological functions of apoA-I may lead to new pharmacological approaches to prevent and/or treat these conditions. ..
  8. APOLIPOPROTEIN VARIATION AND HUMAN DISEASE
    Vassilis Zannis; Fiscal Year: 1999
    ..Thus the information obtained from this project may provide rational approaches towards correcting low plasma HDL levels and hypertriglyceridemia. ..
  9. APOLIPOPROTEIN VARIATION AND HUMAN DISEASE
    Vassilis Zannis; Fiscal Year: 1993
    ..The information which will emerge may then provide rational means of controlling plasma lipoprotein levels in ways that are protective against atherosclerosis...
  10. APOPROTEIN VARIATION AND HUMAN DISEASE
    Vassilis Zannis; Fiscal Year: 1990
    ..The proposed research will enhance our knowledge of new molecular aspects of lipoprotein metabolism which may be relevant to human diseases and will contribute towards better diagnoses and treatment of these conditions...