J Zaia

Summary

Affiliation: Boston University
Country: USA

Publications

  1. ncbi request reprint Influence of charge state on product ion mass spectra and the determination of 4S/6S sulfation sequence of chondroitin sulfate oligosaccharides
    Joseph E McClellan
    Mass Spectrometry Resource, Department of Biochemistry, Boston University School of Medicine, Massachusetts 02118, USA
    Anal Chem 74:3760-71. 2002
  2. pmc GlycReSoft: a software package for automated recognition of glycans from LC/MS data
    Evan Maxwell
    Bioinformatics Program, Center for Biomedical Mass Spectrometry, Department, of Biochemistry, Boston University, Boston, Massachusetts, USA
    PLoS ONE 7:e45474. 2012
  3. pmc Glycomics analysis of mammalian heparan sulfates modified by the human extracellular sulfatase HSulf2
    Gregory O Staples
    Department of Biochemistry, Center for Biomedical Mass Spectrometry, Boston University School of Medicine, Boston, Massachusetts, United States of America
    PLoS ONE 6:e16689. 2011
  4. pmc Glycosaminoglycan glycomics using mass spectrometry
    Joseph Zaia
    Center for Biomedical Mass Spectrometry, Department of Biochemistry, Boston University, Boston, Massachusetts 02118, USA
    Mol Cell Proteomics 12:885-92. 2013
  5. ncbi request reprint Capillary electrophoresis-mass spectrometry of carbohydrates
    Joseph Zaia
    Department of Biochemistry, Center for Biomedical Mass Spectrometry, Boston University Medial Campus, Boston, MA, USA
    Methods Mol Biol 984:13-25. 2013
  6. pmc Mass spectrometry and glycomics
    Joseph Zaia
    Department of Biochemistry, Boston University, Boston, Massachusetts, USA
    OMICS 14:401-18. 2010
  7. ncbi request reprint On-line separations combined with MS for analysis of glycosaminoglycans
    Joseph Zaia
    Department of Biochemistry, Center for Biomedical Mass Spectrometry, Mass Spectrometry Resource, Boston University School of Medicine, Boston, MA 02118, USA
    Mass Spectrom Rev 28:254-72. 2009
  8. pmc Mass spectrometry and the emerging field of glycomics
    Joseph Zaia
    Deptartment of Biochemistry, Boston University, 670 Albany Street, Boston, MA 02118, USA
    Chem Biol 15:881-92. 2008
  9. pmc The role of mobile protons in negative ion CID of oligosaccharides
    Joseph Zaia
    Department of Biochemistry, Mass Spectrometry Resource, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Am Soc Mass Spectrom 18:952-60. 2007
  10. ncbi request reprint Compositional analysis of glycosaminoglycans by electrospray mass spectrometry
    J Zaia
    Mass Spectrometry Resource, Department of Biochemistry, Boston University School of Medicine, Massachusetts 02118, USA
    Anal Chem 73:233-9. 2001

Research Grants

  1. Glycosaminoglycan-Protein Binding
    Joseph Zaia; Fiscal Year: 2003
  2. Glycosaminoglycan-Protein Binding
    Joseph Zaia; Fiscal Year: 2004
  3. Glycosaminoglycan-Protein Binding
    Joseph Zaia; Fiscal Year: 2005
  4. A Thermo Electron LTQ Mass Spectrometer
    Joseph Zaia; Fiscal Year: 2006
  5. Glycosaminoglycan-Protein Binding
    Joseph Zaia; Fiscal Year: 2006
  6. Post-biosynthetic remodeling of heparan sulfate
    Joseph Zaia; Fiscal Year: 2010

Collaborators

  • Catherine Costello
  • Gordon W Slysz
  • Gary Benson
  • Anders Malmstrom
  • Andre Ziegler
  • Gregory O Staples
  • Alicia M Hitchcock
  • Michael J Bowman
  • Hicham Naimy
  • Xiaofeng Shi
  • Alicia M Bielik
  • Nancy Leymarie
  • Evan Maxwell
  • Karen E Yates
  • May Joy C Miller
  • Jennifer L Seymour
  • Estee F Naggar
  • Joseph E McClellan
  • Han Hu
  • Konstantin Aizikov
  • Richard D Smith
  • Shannon Conley
  • Yuxiang Tan
  • Yan Tan
  • Karsten Kraiczek
  • Hongfeng Yin
  • Kevin Kileen
  • James M Lau
  • Christine Miller
  • Sonya Shortkroff
  • PETER B O'CONNOR

Detail Information

Publications32

  1. ncbi request reprint Influence of charge state on product ion mass spectra and the determination of 4S/6S sulfation sequence of chondroitin sulfate oligosaccharides
    Joseph E McClellan
    Mass Spectrometry Resource, Department of Biochemistry, Boston University School of Medicine, Massachusetts 02118, USA
    Anal Chem 74:3760-71. 2002
    ..Sulfate adducted ions were observed in the product ion spectra of singly charged tetramer and hexamer oligosaccharides. This sulfate adduction was determined to result from migration of neutral sulfate during excitation...
  2. pmc GlycReSoft: a software package for automated recognition of glycans from LC/MS data
    Evan Maxwell
    Bioinformatics Program, Center for Biomedical Mass Spectrometry, Department, of Biochemistry, Boston University, Boston, Massachusetts, USA
    PLoS ONE 7:e45474. 2012
    ..We demonstrate this tool, GlycReSoft, using an LC/MS dataset on tissue derived heparan sulfate oligosaccharides. The software, code and a test data set are publically archived under an open source license...
  3. pmc Glycomics analysis of mammalian heparan sulfates modified by the human extracellular sulfatase HSulf2
    Gregory O Staples
    Department of Biochemistry, Center for Biomedical Mass Spectrometry, Boston University School of Medicine, Boston, Massachusetts, United States of America
    PLoS ONE 6:e16689. 2011
    ..Therefore, the present work aims to extend the understanding of substrate specificities of HSulf2 using in vitro experiments to compare HSulf2 activities on HS from different organ tissues...
  4. pmc Glycosaminoglycan glycomics using mass spectrometry
    Joseph Zaia
    Center for Biomedical Mass Spectrometry, Department of Biochemistry, Boston University, Boston, Massachusetts 02118, USA
    Mol Cell Proteomics 12:885-92. 2013
    ..This review summarizes progress on mass-spectrometry-based GAG glycomics methods. The areas covered include disaccharide analysis, oligosaccharide profiling, and tandem mass spectrometric sequencing...
  5. ncbi request reprint Capillary electrophoresis-mass spectrometry of carbohydrates
    Joseph Zaia
    Department of Biochemistry, Center for Biomedical Mass Spectrometry, Boston University Medial Campus, Boston, MA, USA
    Methods Mol Biol 984:13-25. 2013
    ..This chapter summarizes applications of CS/MS to analysis of carbohydrates, glycoproteins, and glycopeptides that have appeared since 2008. Readers are referred to recent comprehensive reviews covering earlier publications...
  6. pmc Mass spectrometry and glycomics
    Joseph Zaia
    Department of Biochemistry, Boston University, Boston, Massachusetts, USA
    OMICS 14:401-18. 2010
    ..Next, the uses of tandem mass spectrometry of glycans are summarized. The review finishes with an update on mass spectral glycoproteomics...
  7. ncbi request reprint On-line separations combined with MS for analysis of glycosaminoglycans
    Joseph Zaia
    Department of Biochemistry, Center for Biomedical Mass Spectrometry, Mass Spectrometry Resource, Boston University School of Medicine, Boston, MA 02118, USA
    Mass Spectrom Rev 28:254-72. 2009
    ..This review summarizes the application of these approaches to on-line MS analysis of the GAG classes...
  8. pmc Mass spectrometry and the emerging field of glycomics
    Joseph Zaia
    Deptartment of Biochemistry, Boston University, 670 Albany Street, Boston, MA 02118, USA
    Chem Biol 15:881-92. 2008
    ..Mass spectrometry (MS) is emerging as an enabling technology in the field of glycomics. This review summarizes recent developments in mass spectrometric analysis methods for protein-based glycomics and glycoproteomics workflows...
  9. pmc The role of mobile protons in negative ion CID of oligosaccharides
    Joseph Zaia
    Department of Biochemistry, Mass Spectrometry Resource, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Am Soc Mass Spectrom 18:952-60. 2007
    ..Mechanisms for product ion formation are proposed based on tandem mass spectra and the abundances of product ions as a function of collision energy...
  10. ncbi request reprint Compositional analysis of glycosaminoglycans by electrospray mass spectrometry
    J Zaia
    Mass Spectrometry Resource, Department of Biochemistry, Boston University School of Medicine, Massachusetts 02118, USA
    Anal Chem 73:233-9. 2001
    ..Mass spectrometric detection produces far more information than conventional UV or fluorescent detectors and allows the monosaccharide composition of individual components to be determined...
  11. ncbi request reprint Tandem mass spectrometric determination of the 4S/6S sulfation sequence in chondroitin sulfate oligosaccharides
    J Zaia
    Mass Spectrometry Resource, Department of Biochemistry, Boston University School of Medicine, Massachusetts 02118, USA
    Anal Chem 73:6030-9. 2001
    ..For charge states where charge equals the number of sulfates, abundant odd-numbered Bn and Yn ions are observed. The percent total ion abundances of these ions indicate the position of sulfation...
  12. ncbi request reprint Mass spectrometry of oligosaccharides
    Joseph Zaia
    Department of Biochemistry, Boston University School of Medicine, 715 Albany St, R 806, Boston, Massachusetts 02118, USA
    Mass Spectrom Rev 23:161-227. 2004
    ..Mass spectrometric techniques for the ionization and fragmentation of oligosaccharides are discussed so as to give the reader the background to make informed decisions to solve structure-activity relations in glycomics...
  13. ncbi request reprint Tandem mass spectrometry of sulfated heparin-like glycosaminoglycan oligosaccharides
    Joseph Zaia
    Department of Biochemistry, Boston University School of Medicine, 715 Albany Street, R 806, Boston, Massachusetts 02118, USA
    Anal Chem 75:2445-55. 2003
    ..Second, calcium ion complexation of HLGAGs stabilizes the sulfate groups, increases the relative abundances of backbone cleavage ions, and decreases the abundances of ions produced from SO3 losses...
  14. ncbi request reprint Tandem mass spectrometric strategies for determination of sulfation positions and uronic acid epimerization in chondroitin sulfate oligosaccharides
    Joseph Zaia
    Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118 2526, USA
    J Am Soc Mass Spectrom 14:1270-81. 2003
    ..The capability of tandem MS to differentiate uronic acid epimers is also shown, providing evidence that complete or nearly complete information on CS covalent structure may be obtained using tandem MS...
  15. pmc Improved workup for glycosaminoglycan disaccharide analysis using CE with LIF detection
    Alicia M Hitchcock
    Department of Biochemistry, Boston University School of Medicine, Boston, MA 02118, USA
    Electrophoresis 29:4538-48. 2008
    ..The improved CE-LIF method enables disaccharide quantification of biologically relevant proteoglycans from small samples of intact tissue...
  16. pmc Comparative glycomics using a tetraplex stable-isotope coded tag
    Michael J Bowman
    Boston University School of Medicine, Department of Biochemistry, Center for Biomedical Mass Spectrometry, Boston, Massachusetts 02118, USA
    Anal Chem 82:3023-31. 2010
    ..The data demonstrate the value of the tetraplex stable isotope tagging approach for producing high-quality glycomics compositional profiling and fine structural analysis...
  17. doi request reprint Historical overview of glycoanalysis
    Alicia M Bielik
    Department of Biochemistry, Boston University School of Medicine, Boston, MA, USA
    Methods Mol Biol 600:9-30. 2010
    ..Mass spectrometry and nuclear magnetic resonance are complementary instrumental techniques for glycan analysis. Finally, microarrays are emerging as powerful new tools for dynamic analysis of glycan expression...
  18. pmc The influence of sialylation on glycan negative ion dissociation and energetics
    Jennifer L Seymour
    Department of Biochemistry, Mass Spectrometry Resource, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Am Soc Mass Spectrom 17:844-54. 2006
    ..These results have strong practical implications because a major portion of glycans released from mammalian proteins will be sialylated...
  19. doi request reprint Extraction of chondroitin/dermatan sulfate glycosaminoglycans from connective tissue for mass spectrometric analysis
    Alicia M Bielik
    Department of Biochemistry, Boston University School of Medicine, Boston, MA, USA
    Methods Mol Biol 600:215-25. 2010
    ..This chapter describes a method for connective tissue GAG extraction, derivatization, and workup for subsequent capillary electrophoretic and/or mass spectrometric analysis...
  20. pmc Extended N-sulfated domains reside at the nonreducing end of heparan sulfate chains
    Gregory O Staples
    Department of Biochemistry, Center for Biomedical Mass Spectrometry, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Biol Chem 285:18336-43. 2010
    ..However, when they do, they tend to terminate in a subset of N-sulfated disaccharides. Our observations are consistent with a significant role of NRE NS domains in HS-growth factor interactions...
  21. pmc A tandem mass spectrometric approach to determination of chondroitin/dermatan sulfate oligosaccharide glycoforms
    May Joy C Miller
    Department of Biochemistry, Boston University School of Medicine, 715 Albany Street, R 806, Boston, MA 02118, USA
    Glycobiology 16:502-13. 2006
    ..Decorin samples ranged from 30% CSB-like repeats for those samples from articular cartilage to 75% for those from sclera. These values agree with known levels of glucuronyl C5-epimerase in these tissues...
  22. pmc Comparative glycomics of connective tissue glycosaminoglycans
    Alicia M Hitchcock
    Department of Biochemistry, Boston University School of Medicine, Boston, MA 02118, USA
    Proteomics 8:1384-97. 2008
    ..The results provide important new information on the changes to the expression of CS GAG chains during disease and development...
  23. pmc Glycoform quantification of chondroitin/dermatan sulfate using a liquid chromatography-tandem mass spectrometry platform
    Alicia M Hitchcock
    Department of Biochemistry, Boston University School of Medicine, 670 Albany Street, Boston, Massachusetts 02118, USA
    Biochemistry 45:2350-61. 2006
    ..This work presents the first application of a glycomics platform for the quantification of CS oligosaccharide mixtures for obtaining specific information about the positions of GalNAc sulfation and uronic acid epimerization...
  24. pmc A chip-based amide-HILIC LC/MS platform for glycosaminoglycan glycomics profiling
    Gregory O Staples
    Department of Biochemistry, Boston University School of Medicine, Mass Spectrometry Resource, Boston, MA 02118, USA
    Proteomics 9:686-95. 2009
    ..In summary, chip based amide-HILIC LC/MS is an enabling technology for GAG glycomics profiling...
  25. ncbi request reprint Size-exclusion chromatography of heparin oligosaccharides at high and low pressure
    Andre Ziegler
    Proteoglycan and Glycosaminoglycan Structure Laboratory, Mass Spectrometry Resource, Boston University, 715 Albany Street, Boston, MA 02118, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 837:76-86. 2006
    ..Optimized SEC affords separation of characteristic heparin trisaccharides that contain uronic acid at the reducing end and suggest cellular storage of heparin as a free glycan...
  26. pmc Optimized extraction of glycosaminoglycans from normal and osteoarthritic cartilage for glycomics profiling
    Alicia M Hitchcock
    Department of Biochemistry, Boston University School of Medicine, MS Resource, 670 Albany Street, Boston, MA 02118, USA
    Glycobiology 17:25-35. 2007
    ..These results show that the new method has sufficient accuracy to allow determination of topographical distribution of glycoforms in connective tissue...
  27. pmc Tags for the stable isotopic labeling of carbohydrates and quantitative analysis by mass spectrometry
    Michael J Bowman
    Department of Biochemistry, Center for Biomedical Mass Spectrometry, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    Anal Chem 79:5777-84. 2007
    ..Quantitation of partially depolymerized glycosaminoglycan mixtures, as well as N-linked glycans released from fetuin, is used to demonstrate the utility of the tetraplex tagging strategy...
  28. pmc Characterization of heparin oligosaccharides binding specifically to antithrombin III using mass spectrometry
    Hicham Naimy
    Department of Biochemistry, Boston University School of Medicine, 670 Albany Street, Boston, Massachusetts 02118, USA
    Biochemistry 47:3155-61. 2008
    ..The specificity of the hexasaccharides binding ATIII was confirmed by assaying their ability to enhance ATIII-mediated inhibition of Factor Xa in vitro...
  29. pmc Screening for anticoagulant heparan sulfate octasaccharides and fine structure characterization using tandem mass spectrometry
    Hicham Naimy
    Department of Biochemistry, Boston University School of Medicine, 670 Albany Street, Boston, Massachusetts 02118, USA
    Biochemistry 49:3743-52. 2010
    ..This approach confirmed isomeric enrichment of these compositions and, most importantly, produces ions diagnostic of their biological activity...
  30. pmc Improved hydrophilic interaction chromatography LC/MS of heparinoids using a chip with postcolumn makeup flow
    Gregory O Staples
    Mass Spectrometry Resource, Department of Biochemistry, Boston University School of Medicine, 670 Albany Street, Boston, Massachusetts 02118, USA
    Anal Chem 82:516-22. 2010
    ..It is expected that this technology will enable quantitative, comparative glycomics profiling of extended GAG oligosaccharide domains of functional interest...
  31. ncbi request reprint Competing fragmentation processes in tandem mass spectra of heparin-like glycosaminoglycans
    Estee F Naggar
    Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118 2526, USA
    J Am Soc Mass Spectrom 15:1534-44. 2004
    ....
  32. pmc Organ-specific heparan sulfate structural phenotypes
    Xiaofeng Shi
    Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Biol Chem 284:11806-14. 2009
    ..The data show the presence of a disaccharide with an unsubstituted amino group that is endogenous and widely expressed in mammalian organ tissues...

Research Grants6

  1. Glycosaminoglycan-Protein Binding
    Joseph Zaia; Fiscal Year: 2003
    ..abstract_text> ..
  2. Glycosaminoglycan-Protein Binding
    Joseph Zaia; Fiscal Year: 2004
    ..abstract_text> ..
  3. Glycosaminoglycan-Protein Binding
    Joseph Zaia; Fiscal Year: 2005
    ..abstract_text> ..
  4. A Thermo Electron LTQ Mass Spectrometer
    Joseph Zaia; Fiscal Year: 2006
    ..The costs of instrument operation, supplies, and service contract will be shared by the users, as stated in their support letters. The salary for the instrument operator will be provided by the Dean of BU School of Medicine. ..
  5. Glycosaminoglycan-Protein Binding
    Joseph Zaia; Fiscal Year: 2006
    ..abstract_text> ..
  6. Post-biosynthetic remodeling of heparan sulfate
    Joseph Zaia; Fiscal Year: 2010
    ..This research will explore the structure-function relationships of vascular heparan sulfates in order to inform efforts to develop protein binding microarrays, drugs and therapeutics. ..