David Waxman

Summary

Affiliation: Boston University
Country: USA

Publications

  1. pmc Male-specific hepatic Bcl6: growth hormone-induced block of transcription elongation in females and binding to target genes inversely coordinated with STAT5
    Rosana D Meyer
    Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, Massachusetts 02215, USA
    Mol Endocrinol 23:1914-26. 2009
  2. ncbi Adenoviral Vectors for Prodrug Activation-based Gene Therapy for Cancer
    Joshua C Doloff
    Department of Cell and Molecular, Biology, Boston University, 5 Cummington Mall, Boston, MA 02215, USA
    Anticancer Agents Med Chem 14:115-26. 2014
  3. pmc Impact of tumor vascularity on responsiveness to antiangiogenesis in a prostate cancer stem cell-derived tumor model
    Kexiong Zhang
    Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, Massachusetts, USA
    Mol Cancer Ther 12:787-98. 2013
  4. pmc Impact of tumor blood flow modulation on tumor sensitivity to the bioreductive drug banoxantrone
    Eugene Manley
    Department of Biology, Boston University, 5 Cummington Street, Boston, MA 02215, USA
    J Pharmacol Exp Ther 344:368-77. 2013
  5. pmc Sex-specific mouse liver gene expression: genome-wide analysis of developmental changes from pre-pubertal period to young adulthood
    Tara L Conforto
    Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, MA 02215, USA
    Biol Sex Differ 3:9. 2012
  6. pmc MAnorm: a robust model for quantitative comparison of ChIP-Seq data sets
    Zhen Shao
    Department of Pediatric Oncology, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Genome Biol 13:R16. 2012
  7. pmc Dynamic, sex-differential STAT5 and BCL6 binding to sex-biased, growth hormone-regulated genes in adult mouse liver
    Yijing Zhang
    Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, Massachusetts, USA
    Mol Cell Biol 32:880-96. 2012
  8. pmc Complex modulation of androgen responsive gene expression by methoxyacetic acid
    Gargi Bagchi
    Department of Biology, Boston University, Boston, MA 02215, USA
    Reprod Biol Endocrinol 9:42. 2011
  9. pmc Impact of methoxyacetic acid on mouse Leydig cell gene expression
    Gargi Bagchi
    Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, MA 02215, USA
    Reprod Biol Endocrinol 8:65. 2010
  10. pmc PC3 prostate tumor-initiating cells with molecular profile FAM65Bhigh/MFI2low/LEF1low increase tumor angiogenesis
    Kexiong Zhang
    Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, MA 02215, USA
    Mol Cancer 9:319. 2010

Research Grants

  1. Cytochrome P450-Endogenous Substrate Metabolism
    David J Waxman; Fiscal Year: 2010
  2. Hepatic Metabolism of Anti-cancer Drugs
    David Waxman; Fiscal Year: 2007
  3. Cytochrome P450-Endogenous Substrate Metabolism
    David J Waxman; Fiscal Year: 2010
  4. Cytochrome P450-Endogenous Substrate Metabolism
    David J Waxman; Fiscal Year: 2010
  5. Cytochrome P450-Endogenous Substrate Metabolism
    David Waxman; Fiscal Year: 2006
  6. Cytochrome P450-Endogenous Substrate Metabolism
    David Waxman; Fiscal Year: 2007
  7. Hepatic Metabolism of Anti-cancer Drugs
    David Waxman; Fiscal Year: 2007
  8. Combination Therapies for Cancer Treatment
    David J Waxman; Fiscal Year: 2010
  9. Cytochrome P450-Endogenous Substrate Metabolism
    David Waxman; Fiscal Year: 2009
  10. Combination Therapies for Cancer Treatment
    David J Waxman; Fiscal Year: 2011

Collaborators

Detail Information

Publications82

  1. pmc Male-specific hepatic Bcl6: growth hormone-induced block of transcription elongation in females and binding to target genes inversely coordinated with STAT5
    Rosana D Meyer
    Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, Massachusetts 02215, USA
    Mol Endocrinol 23:1914-26. 2009
    ..Bcl6 and STAT5 binding are thus inversely coordinated by the endogenous pulses of pituitary GH release, suggesting this male-specific transcriptional repressor modulates hepatic GH signaling to select STAT5 target genes...
  2. ncbi Adenoviral Vectors for Prodrug Activation-based Gene Therapy for Cancer
    Joshua C Doloff
    Department of Cell and Molecular, Biology, Boston University, 5 Cummington Mall, Boston, MA 02215, USA
    Anticancer Agents Med Chem 14:115-26. 2014
    ....
  3. pmc Impact of tumor vascularity on responsiveness to antiangiogenesis in a prostate cancer stem cell-derived tumor model
    Kexiong Zhang
    Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, Massachusetts, USA
    Mol Cancer Ther 12:787-98. 2013
    ..Finally, prolonged antiangiogenic treatment led to resumption of PC3/2G7 tumor growth and neovascularization, indicating these cancer stem-like cell-derived tumors can adapt and escape from antiangiogenesis...
  4. pmc Impact of tumor blood flow modulation on tumor sensitivity to the bioreductive drug banoxantrone
    Eugene Manley
    Department of Biology, Boston University, 5 Cummington Street, Boston, MA 02215, USA
    J Pharmacol Exp Ther 344:368-77. 2013
    ..Moreover, the activation of AQ4N cytotoxicity in vivo requires tumor hypoxia that is more extensive or prolonged than can readily be achieved by vasodilation or by antiangiogenic drug treatment...
  5. pmc Sex-specific mouse liver gene expression: genome-wide analysis of developmental changes from pre-pubertal period to young adulthood
    Tara L Conforto
    Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, MA 02215, USA
    Biol Sex Differ 3:9. 2012
    ..abstract:..
  6. pmc MAnorm: a robust model for quantitative comparison of ChIP-Seq data sets
    Zhen Shao
    Department of Pediatric Oncology, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Genome Biol 13:R16. 2012
    ..The quantitative binding differences inferred by MAnorm showed strong correlation with both the changes in expression of target genes and the binding of cell type-specific regulators...
  7. pmc Dynamic, sex-differential STAT5 and BCL6 binding to sex-biased, growth hormone-regulated genes in adult mouse liver
    Yijing Zhang
    Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, Massachusetts, USA
    Mol Cell Biol 32:880-96. 2012
    ..The approaches used here to characterize sex-dependent STAT5 and BCL6 binding can be applied to other condition-specific regulatory factors and binding sites and their interplay with cooperative chromatin binding factors...
  8. pmc Complex modulation of androgen responsive gene expression by methoxyacetic acid
    Gargi Bagchi
    Department of Biology, Boston University, Boston, MA 02215, USA
    Reprod Biol Endocrinol 9:42. 2011
    ....
  9. pmc Impact of methoxyacetic acid on mouse Leydig cell gene expression
    Gargi Bagchi
    Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, MA 02215, USA
    Reprod Biol Endocrinol 8:65. 2010
    ..This study investigates the impact of MAA on gene expression in testicular Leydig cells, which play a critical role in germ cell survival and male reproductive function...
  10. pmc PC3 prostate tumor-initiating cells with molecular profile FAM65Bhigh/MFI2low/LEF1low increase tumor angiogenesis
    Kexiong Zhang
    Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, MA 02215, USA
    Mol Cancer 9:319. 2010
    ....
  11. pmc Adenoviral delivery of pan-caspase inhibitor p35 enhances bystander killing by P450 gene-directed enzyme prodrug therapy using cyclophosphamide+
    Joshua C Doloff
    Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, MA 02215, USA
    BMC Cancer 10:487. 2010
    ..Previous studies have shown that the pan-caspase inhibitor p35 significantly increases CPA-induced bystander killing by tumor cells that stably express P450 enzyme CYP2B6 (Schwartz et al, (2002) Cancer Res. 62: 6928-37)...
  12. pmc Sex differences in the expression of hepatic drug metabolizing enzymes
    David J Waxman
    Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, MA 02215, USA
    Mol Pharmacol 76:215-28. 2009
    ....
  13. ncbi Harnessing apoptosis for improved anticancer gene therapy
    David J Waxman
    Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, Massachusetts 02215, USA
    Cancer Res 63:8563-72. 2003
    ....
  14. ncbi Growth hormone regulation of sex-dependent liver gene expression
    David J Waxman
    Department of Biology, Boston University, 5 Cummington Street, Boston, Massachusetts 02215, USA
    Mol Endocrinol 20:2613-29. 2006
    ....
  15. ncbi Identification of novel enzyme-prodrug combinations for use in cytochrome P450-based gene therapy for cancer
    Alex Baldwin
    Division of Cell and Molecular Biology, Department of Biology, Boston University, 5 Cummington St, MA 02215, USA
    Arch Biochem Biophys 409:197-206. 2003
    ....
  16. pmc Collaboration between hepatic and intratumoral prodrug activation in a P450 prodrug-activation gene therapy model for cancer treatment
    Jie Ma
    Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, MA 02215, USA
    Mol Cancer Ther 6:2879-90. 2007
    ....
  17. ncbi Use of replication-conditional adenovirus as a helper system to enhance delivery of P450 prodrug-activation genes for cancer therapy
    Youssef Jounaidi
    Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, Massachusetts 02215, USA
    Cancer Res 64:292-303. 2004
    ....
  18. ncbi Sustained P450 expression and prodrug activation in bolus cyclophosphamide-treated cultured tumor cells. Impact of prodrug schedule on P450 gene-directed enzyme prodrug therapy
    Pamela S Schwartz
    Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, Massachussetts 02215, USA
    Cancer Gene Ther 10:571-82. 2003
    ....
  19. ncbi Role of hepatocyte nuclear factors in growth hormone-regulated, sexually dimorphic expression of liver cytochromes P450
    Christopher A Wiwi
    Division of Cell and Molecular Biology, Department of Biology, Boston University, 5 Cummington Street, Boston, MA 02215, USA
    Growth Factors 22:79-88. 2004
    ....
  20. ncbi Role of hepatocyte nuclear factors in transcriptional regulation of male-specific CYP2A2
    Christopher A Wiwi
    Division of Cell and Molecular Biology, Department of Biology Boston University, Boston, Massachusetts 02215, USA
    J Biol Chem 280:3259-68. 2005
    ....
  21. ncbi Activation of the anticancer prodrugs cyclophosphamide and ifosfamide: identification of cytochrome P450 2B enzymes and site-specific mutants with improved enzyme kinetics
    Chong Sheng Chen
    Department of Biology, Boston University, Boston, MA 02215, USA
    Mol Pharmacol 65:1278-85. 2004
    ....
  22. ncbi Environmental and endogenous peroxisome proliferator-activated receptor gamma agonists induce bone marrow B cell growth arrest and apoptosis: interactions between mono(2-ethylhexyl)phthalate, 9-cis-retinoic acid, and 15-deoxy-Delta12,14-prostaglandin J2
    Jennifer J Schlezinger
    Department of Environmental Health, Boston University School of Public Health, Boston, MA 02118, USA
    J Immunol 173:3165-77. 2004
    ..These data demonstrate that environmental phthalates can cooperate with an endogenous ligand, 15d-PGJ(2), to inhibit proliferation of and induce apoptosis in developing bone marrow B cells, potentially via PPARgamma activation...
  23. ncbi Enhanced antitumor activity of P450 prodrug-based gene therapy using the low Km cyclophosphamide 4-hydroxylase P450 2B11
    Youssef Jounaidi
    Division of Cell and Molecular Biology, Department of Biology, Boston University, 5 Cummington Street, Boston, MA 02215, USA
    Mol Cancer Ther 5:541-55. 2006
    ..Thus, P450 gene-directed enzyme prodrug therapy can be greatly improved by using the low Km P450 enzyme 2B11, which catalyzes intratumoral activation of cyclophosphamide and ifosfamide at pharmacologically relevant drug concentrations...
  24. ncbi Enantioselective metabolism and cytotoxicity of R-ifosfamide and S-ifosfamide by tumor cell-expressed cytochromes P450
    Chong Sheng Chen
    Division of Cell and Molocular Biology, Department of Biology, Boston University, Boston, MA 02215, USA
    Drug Metab Dispos 33:1261-7. 2005
    ....
  25. pmc Conditionally replicating adenoviruses for cancer treatment
    Youssef Jounaidi
    Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, MA 02215, USA
    Curr Cancer Drug Targets 7:285-301. 2007
    ..The continued expansion of the knowledge base of adenovirus biology will likely lead to further improvements in the design of the ideal oncolytic adenoviruses for cancer treatment...
  26. ncbi Environmental phthalate monoesters activate pregnane X receptor-mediated transcription
    Christopher H Hurst
    Division of Cell and Molecular Biology, Department of Biology, Boston University, MA 02215, USA
    Toxicol Appl Pharmacol 199:266-74. 2004
    ....
  27. ncbi Enhanced bystander cytotoxicity of P450 gene-directed enzyme prodrug therapy by expression of the antiapoptotic factor p35
    Pamela S Schwartz
    Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, Massachusetts 02215, USA
    Cancer Res 62:6928-37. 2002
    ....
  28. ncbi Simultaneous, bidirectional inhibitory crosstalk between PPAR and STAT5b
    Jonathan M Shipley
    Division of Cell and Molecular Biology, Department of Biology, Boston University, MA 02215, USA
    Toxicol Appl Pharmacol 199:275-84. 2004
    ..Conversely, STAT5-activating hormones and cytokines may modulate the responsiveness of PPARs to their foreign chemical ligands...
  29. ncbi Sexually dimorphic P450 gene expression in liver-specific hepatocyte nuclear factor 4alpha-deficient mice
    Christopher A Wiwi
    Department of Biology, Boston University, 5 Cummington Street, Boston, Massachusetts 02215, USA
    Mol Endocrinol 18:1975-87. 2004
    ....
  30. pmc Unbiased, genome-wide in vivo mapping of transcriptional regulatory elements reveals sex differences in chromatin structure associated with sex-specific liver gene expression
    Guoyu Ling
    Department of Biology, Boston University, 5 Cummington Street, Boston, MA 02215, USA
    Mol Cell Biol 30:5531-44. 2010
    ..This approach can readily be applied to mapping condition-specific regulatory sites in mammalian tissues under a wide variety of physiological conditions...
  31. pmc Characterization of three growth hormone-responsive transcription factors preferentially expressed in adult female liver
    Ekaterina V Laz
    Department of Biology, Boston University, 5 Cummington Street, Boston, Massachusetts 02215, USA
    Endocrinology 148:3327-37. 2007
    ..Binding sites for Cutl1, whose DNA-binding specificity is close to that of Cutl2, were statistically overrepresented in STAT5b-dependent male-specific mouse genes, lending support to this hypothesis...
  32. ncbi Cytochrome p450-based gene therapies for cancer
    E Antonio Chiocca
    Molecular Neuro oncology Laboratory, Neurosurgery Service, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA
    Methods Mol Med 90:203-22. 2004
  33. pmc Sex-specific early growth hormone response genes in rat liver
    Valerie Wauthier
    Department of Biology, Boston University, 5 Cummington Street, Boston, Massachusetts 02215, USA
    Mol Endocrinol 22:1962-74. 2008
    ..We conclude that GH acts via both positive and negative regulatory mechanisms to establish and maintain the sex specificity of liver gene expression...
  34. ncbi Elevated basal expression of liver peroxisomal beta-oxidation enzymes and CYP4A microsomal fatty acid omega-hydroxylase in STAT5b(-/-) mice: cross-talk in vivo between peroxisome proliferator-activated receptor and signal transducer and activator of trans
    Yuan Chun Zhou
    Department of Biology, Division of Cell and Molecular Biology, Boston University, Boston, Massachusetts 02215, USA
    Toxicol Appl Pharmacol 182:1-10. 2002
    ....
  35. ncbi Sexual dimorphism of rat liver gene expression: regulatory role of growth hormone revealed by deoxyribonucleic Acid microarray analysis
    Amrita Ahluwalia
    Division of Cell and Molecular Biology, Department of Biology, Boston University, 5 Cummington Street, Boston, Massachusetts 02215, USA
    Mol Endocrinol 18:747-60. 2004
    ..Taken together, these studies establish that GH-regulated gene expression is the major mechanistic determinant of sexually dimorphic gene expression in the rat liver model...
  36. ncbi Down-regulation of STAT5b transcriptional activity by ligand-activated peroxisome proliferator-activated receptor (PPAR) alpha and PPARgamma
    Jonathan M Shipley
    Department of Biology, Boston University, 5 Cummington St, Boston, MA 02215, USA
    Mol Pharmacol 64:355-64. 2003
    ..These findings demonstrate the bidirectionality of cross-talk between the PPAR and STAT pathways and provide a mechanism whereby exposure to environmental chemical activators of PPAR can suppress expression of GH target genes...
  37. ncbi Activation of PPARalpha and PPARgamma by environmental phthalate monoesters
    Christopher H Hurst
    Department of Biology, Division of Cell and Molecular Biology, Boston University, Boston, Massachusetts 02215, USA
    Toxicol Sci 74:297-308. 2003
    ....
  38. pmc Signalling cross-talk between hepatocyte nuclear factor 4alpha and growth-hormone-activated STAT5b
    Soo Hee Park
    Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, MA 02215, USA
    Biochem J 397:159-68. 2006
    ....
  39. ncbi Use of 7-ethoxycoumarin to monitor multiple enzymes in the human CYP1, CYP2, and CYP3 families
    David J Waxman
    Department of Biology, Boston University, MA, USA
    Methods Mol Biol 320:153-6. 2006
    ....
  40. pmc Role of STAT5a in regulation of sex-specific gene expression in female but not male mouse liver revealed by microarray analysis
    Karl H Clodfelter
    Division of Cell and Molecular Biology, Department of Biology, Boston University, Massachusetts, USA
    Physiol Genomics 31:63-74. 2007
    ..These findings highlight the importance of STAT5a for regulation of sex-specific gene expression specifically in female liver, in striking contrast to STAT5b, whose major effects are restricted to male liver...
  41. ncbi Sexual dimorphism of rat liver nuclear proteins: regulatory role of growth hormone
    Ekaterina V Laz
    Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, MA 02215, USA
    Mol Cell Proteomics 3:1170-80. 2004
    ....
  42. pmc Loss of sexually dimorphic liver gene expression upon hepatocyte-specific deletion of Stat5a-Stat5b locus
    Minita G Holloway
    Department of Biology, Boston University, Boston, Massachusetts 02215, USA
    Endocrinology 148:1977-86. 2007
    ..Thus, the major loss of liver sexual dimorphism in hepatocyte STAT5ab-deficient mice can primarily be attributed to the loss of STAT5b...
  43. pmc Modulation of the antitumor activity of metronomic cyclophosphamide by the angiogenesis inhibitor axitinib
    Jie Ma
    Division of Cell and Molecular Biology, Department of Biology, Boston University, 5 Cummington Street, Boston, MA 02215, USA
    Mol Cancer Ther 7:79-89. 2008
    ....
  44. ncbi Impact of dimethyl sulfoxide on expression of nuclear receptors and drug-inducible cytochromes P450 in primary rat hepatocytes
    Ting Su
    Department of Biology, Division of Cell and Molecular Biology, Boston University, 5 Cummington Street, Boston, MA 02215, USA
    Arch Biochem Biophys 424:226-34. 2004
    ....
  45. ncbi Sex-dependent liver gene expression is extensive and largely dependent upon signal transducer and activator of transcription 5b (STAT5b): STAT5b-dependent activation of male genes and repression of female genes revealed by microarray analysis
    Karl H Clodfelter
    Department of Biology, Boston University, 5 Cummington Street, Boston, Massachusetts 02215, USA
    Mol Endocrinol 20:1333-51. 2006
    ..Several female-predominant repressors are elevated in STAT5b-deficient males; these may contribute to the major loss of male gene expression seen in the absence of STAT5b...
  46. ncbi Codependence of growth hormone-responsive, sexually dimorphic hepatic gene expression on signal transducer and activator of transcription 5b and hepatic nuclear factor 4alpha
    Minita G Holloway
    Division of Cell and Molecular Biology, Department of Biology, Boston University, 5 Cummington Street, Boston, Massachusetts 02215, USA
    Mol Endocrinol 20:647-60. 2006
    ....
  47. ncbi Spectrofluorometric analysis of CYP2A6-catalyzed coumarin 7-hydroxylation
    David J Waxman
    Department of Biology, Boston University, MA, USA
    Methods Mol Biol 320:91-6. 2006
    ....
  48. ncbi Antitumor activity of methoxymorpholinyl doxorubicin: potentiation by cytochrome P450 3A metabolism
    Hong Lu
    Department of Biology, Boston University, 5 Cummington Street, Boston, MA 02215, USA
    Mol Pharmacol 67:212-9. 2005
    ....
  49. pmc Combination of antiangiogenesis with chemotherapy for more effective cancer treatment
    Jie Ma
    Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, MA 02215, USA
    Mol Cancer Ther 7:3670-84. 2008
    ..New targets for antiangiogenesis continue to be discovered, increasing the opportunities to interdict tumor angiogenesis and circumvent resistance mechanisms that may emerge with chronic use of these drugs...
  50. pmc Dynamic in vivo binding of STAT5 to growth hormone-regulated genes in intact rat liver. Sex-specific binding at low- but not high-affinity STAT5 sites
    Ekaterina V Laz
    Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, Massachusetts 02215, USA
    Mol Endocrinol 23:1242-54. 2009
    ....
  51. ncbi Aryl hydrocarbon receptor-independent activation of estrogen receptor-dependent transcription by 3-methylcholanthrene
    Jonathan M Shipley
    Division of Cell and Molecular Biology, Department of Biology, Boston University, 5 Cummington Street, MA 02215, USA
    Toxicol Appl Pharmacol 213:87-97. 2006
    ..3MC may thus elicit estrogenic activity by multiple mechanisms...
  52. ncbi Computational solvent mapping reveals the importance of local conformational changes for broad substrate specificity in mammalian cytochromes P450
    Karl H Clodfelter
    Bioinformatics Program, Boston University, 44 Cummington Street, Boston, Massachusetts 02215, USA
    Biochemistry 45:9393-407. 2006
    ..In addition, the binding of S-warfarin to P450 2C9 creates a high-affinity site for a second ligand, which may help to explain the prevalence of drug-drug interactions involving this and other mammalian P450s...
  53. ncbi Sexual dimorphism of hepatic gene expression: novel biological role of KRAB zinc finger repressors revealed
    David J Waxman
    Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, Massachusetts 02215, USA
    Genes Dev 17:2607-13. 2003
  54. pmc Intrinsic sex differences in the early growth hormone responsiveness of sex-specific genes in mouse liver
    Valerie Wauthier
    Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, Massachusetts 02215, USA
    Mol Endocrinol 24:667-78. 2010
    ....
  55. ncbi Computational screening of phthalate monoesters for binding to PPARgamma
    Taner Kaya
    Department of Chemistry, Boston University, Boston, Massachusetts 02215, USA
    Chem Res Toxicol 19:999-1009. 2006
    ..These findings support the use of computational methods to identify environmental chemicals that warrant further experimental evaluation for PPAR binding and trans-activation potential in cell-based models...
  56. ncbi Evaluation of thyroid hormone effects on liver P450 reductase translation
    Ekaterina V Apletalina
    Department of Biology, Boston University, 5 Cummington Street, MA 02215, USA
    Arch Biochem Biophys 409:172-9. 2003
    ..Taken together, these data suggest that P450R may in part be regulated at the level of protein stability in hyperthyroid rat liver...
  57. pmc Dominant effect of antiangiogenesis in combination therapy involving cyclophosphamide and axitinib
    Jie Ma
    Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, Massachusetts 02215, USA
    Clin Cancer Res 15:578-88. 2009
    ..Furthermore, the effect of axitinib on the antitumor activity of combination treatments with cyclophosphamide was examined...
  58. ncbi Post-transcriptional regulation of hepatic NADPH-cytochrome P450 reductase by thyroid hormone: independent effects on poly(A) tail length and mRNA stability
    Dongxu Liu
    Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, Massachusetts, USA
    Mol Pharmacol 61:1089-96. 2002
    ....
  59. pmc Interactions of methoxyacetic acid with androgen receptor
    Gargi Bagchi
    Division of Cell and Molecular Biology, Department of Biology, Boston University, 5 Cummington Street, Boston, MA 02215, USA
    Toxicol Appl Pharmacol 238:101-10. 2009
    ..Deregulation of these genes may alter androgen synthesis and action in a manner that contributes to MAA-induced testicular toxicity...
  60. ncbi trans-activation of PPARalpha and induction of PPARalpha target genes by perfluorooctane-based chemicals
    Jonathan M Shipley
    Division of Cell and Molecular Biology, Department of Biology, Boston University, 5 Cummington Street, Boston, Massachusetts 02215, USA
    Toxicol Sci 80:151-60. 2004
    ....
  61. pmc Liver-specific hepatocyte nuclear factor-4alpha deficiency: greater impact on gene expression in male than in female mouse liver
    Minita G Holloway
    Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, Massachusetts 02215, USA
    Mol Endocrinol 22:1274-86. 2008
    ..Thus, a substantial fraction of the HNF4alpha-dependent genes reported here are likely to be direct targets of HNF4alpha...
  62. ncbi Exploring the binding site structure of the PPAR gamma ligand-binding domain by computational solvent mapping
    Shu Hsien Sheu
    Department of Biomedical Engineering, Boston University, 44 Cummington Street, Boston, Massachusetts 02215, USA
    Biochemistry 44:1193-209. 2005
    ..The number of probe clusters retained by these sites depends on the properties of the bound agonist, providing information on the origin of correlations between ligand and coactivator binding...
  63. pmc Human telomerase reverse transcriptase promoter-driven oncolytic adenovirus with E1B-19 kDa and E1B-55 kDa gene deletions
    Joshua C Doloff
    Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, MA 02215, USA
    Hum Gene Ther 19:1383-400. 2008
    ..Adeno-hTERT-E1A thus has strong therapeutic potential and an improved safety profile compared with ONYX-015, which may lead to reduced toxicity in the clinic...
  64. pmc The structural basis of pregnane X receptor binding promiscuity
    Chi Ho Ngan
    Department of Biomedical Engineering, Boston University, Boston, Massachusetts 02215, USA
    Biochemistry 48:11572-81. 2009
    ..Results also suggest a unique signal transduction mechanism between the PXR homodimerization interface and its coactivator binding site...
  65. ncbi Role of the cytokine-induced SH2 domain-containing protein CIS in growth hormone receptor internalization
    Tanya Landsman
    Division of Cell and Molecular Biology, Department of Biology, Boston University, MA 02215, USA
    J Biol Chem 280:37471-80. 2005
    ....
  66. ncbi Activation of oxazaphosphorines by cytochrome P450: application to gene-directed enzyme prodrug therapy for cancer
    Partha Roy
    Forest Research Institute, A Division of Forest Laboratories, Inc, Harborside Financial Center, Plaza V, Jersey City, NJ 07311, USA
    Toxicol In Vitro 20:176-86. 2006
    ..Recent advances in this area of research are reviewed, and two proof-of-concept clinical trials that highlight the utility of this strategy are discussed...
  67. ncbi Synthetic drugs and natural products as modulators of constitutive androstane receptor (CAR) and pregnane X receptor (PXR)
    Thomas K H Chang
    Faculty of Pharmaceutical Sciences, The University of British Columbia, Vancouver, Canada
    Drug Metab Rev 38:51-73. 2006
    ..This article discusses the importance of these receptors for pharmacology and toxicology, emphasizing the role of individual drugs and natural products as agonists, indirect activators, inverse agonists, and antagonists of CAR and PXR...
  68. ncbi Growth hormone determines sexual dimorphism of hepatic cytochrome P450 3A4 expression in transgenic mice
    Connie Cheung
    Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Building 37, Room 3106, Bethesda, MD 20892, USA
    J Pharmacol Exp Ther 316:1328-34. 2006
    ....
  69. ncbi Cytochrome P450 gene-directed enzyme prodrug therapy (GDEPT) for cancer
    Ling Chen
    Department of Gene Transfer Technologies and Therapeutics, Lexicon Genetics Inc, The Woodlands, TX 77381, USA
    Curr Pharm Des 8:1405-16. 2002
    ..A recent report of clinical efficacy in a P450-based phase I/II gene therapy trial for pancreatic cancer patients supports this conclusion...
  70. pmc Re-engineering cytochrome P450 2B11dH for enhanced metabolism of several substrates including the anti-cancer prodrugs cyclophosphamide and ifosfamide
    Ling Sun
    Department of Pharmacology and Toxicology, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555 1031, USA
    Arch Biochem Biophys 458:167-74. 2007
    ..Overall, the re-engineered P450 2B11dH enzymes exhibited enhanced catalytic efficiency with several substrates including the anti-cancer prodrugs...
  71. ncbi Directed evolution of mammalian cytochrome P450 2B1: mutations outside of the active site enhance the metabolism of several substrates, including the anticancer prodrugs cyclophosphamide and ifosfamide
    Santosh Kumar
    Department of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston, 77555, USA
    J Biol Chem 280:19569-75. 2005
    ..5-fold enhancement with ifosfamide. Directed evolution can thus be used to enhance P450 2B1 catalytic efficiency across a panel of substrates and to identify functionally important residues distant from the active site...
  72. ncbi Cancer chemotherapy and drug metabolism
    David S Riddick
    Department of Pharmacology, Medical Sciences Building, University of Toronto, Toronto, Ontario, Canada
    Drug Metab Dispos 33:1083-96. 2005
    ..A clear theme emerged from this symposium: drug metabolism and transport processes can be modulated and exploited in ways that may offer distinct therapeutic advantages in the management of patients with cancer...
  73. pmc A mouse model with liver-specific deletion and global suppression of the NADPH-cytochrome P450 reductase gene: characterization and utility for in vivo studies of cyclophosphamide disposition
    Jun Gu
    Wadsworth Center, New York State Department of Health, Empire State Plaza, Box 509, Albany, NY 12201 0509, USA
    J Pharmacol Exp Ther 321:9-17. 2007
    ....
  74. ncbi Enzymatic analysis of cDNA-expressed human CYP1A1, CYP1A2, and CYP1B1 with 7-ethoxyresorufin as substrate
    Thomas K H Chang
    Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, Canada
    Methods Mol Biol 320:85-90. 2006
    ..It can also be used to assay 7-ethoxyresorufin O-dealkylation activity in isolated hepatocytes and cultured cells that express this P450 activity...
  75. ncbi Pregnane X receptor-mediated transcription
    Thomas K H Chang
    Faculty of Pharmaceutical Sciences, The University of British Columbia, Vancouver, Canada
    Methods Enzymol 400:588-98. 2005
    ....
  76. ncbi Catalytic assays for human cytochrome P450: an introduction
    Thomas K H Chang
    Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, Canada
    Methods Mol Biol 320:73-83. 2006
    ..Cautions that need to be exercised when using these substrates to probe for individual P450 activities in human liver and other tissues are discussed...
  77. ncbi Spectrophotometric analysis of human CYP2E1-catalyzed p-nitrophenol hydroxylation
    Thomas K H Chang
    Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, Canada
    Methods Mol Biol 320:127-31. 2006
    ..This method is applicable to enzymatic studies for determination of P450-catalyzed p-nitrophenol hydroxylation activity...
  78. ncbi Determination of CYP2B6 component of 7-ethoxy-4-trifluoromethylcoumarin O-deethylation activity in human liver microsomes
    Thomas K H Chang
    Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, Canada
    Methods Mol Biol 320:97-102. 2006
    ..This approach can be modified to assay the catalytic activity of cDNA-expressed CYP2B6...
  79. ncbi High-performance liquid chromatography analysis of CYP2C8-catalyzed paclitaxel 6alpha-hydroxylation
    Charles L Crespi
    Discovery Labware, BD Biosciences, Woburn, MA, USA
    Methods Mol Biol 320:103-7. 2006
    ..This method is applicable to enzymatic studies for determination of CYP2C8-catalyzed paclitaxel 6alpha-hydroxylation activity...
  80. ncbi Determination of CYP4A11-catalyzed lauric acid 12-hydroxylation by high-performance liquid chromatography with radiometric detection
    Charles L Crespi
    Discovery Labware, BD Biosciences, Woburn, MA, USA
    Methods Mol Biol 320:143-7. 2006
    ..This method is applicable to enzymatic studies for determination of lauric acid 12-hydroxylation activity...
  81. ncbi CYP2C19-mediated (S)-mephenytoin 4'-hydroxylation assayed by high-performance liquid chromatography with radiometric detection
    Charles L Crespi
    Discovery Labware, BD Biosciences, Woburn, MA, USA
    Methods Mol Biol 320:115-9. 2006
    ..This method is applicable to enzymatic studies for determination of CYP2C19-catalyzed (S)-mephenytoin 4'-hydroxylation activity...
  82. ncbi Determination of CYP2C9-catalyzed diclofenac 4'-hydroxylation by high-performance liquid chromatography
    Charles L Crespi
    Discovery Labware, BD Biosciences, Woburn, MA, USA
    Methods Mol Biol 320:109-13. 2006
    ..This method is applicable to enzymatic studies for determination of CYP2C9-catalyzed diclofenac 4'-hydroxylation activity...

Research Grants38

  1. Cytochrome P450-Endogenous Substrate Metabolism
    David J Waxman; Fiscal Year: 2010
    ....
  2. Hepatic Metabolism of Anti-cancer Drugs
    David Waxman; Fiscal Year: 2007
    ..abstract_text> ..
  3. Cytochrome P450-Endogenous Substrate Metabolism
    David J Waxman; Fiscal Year: 2010
    ....
  4. Cytochrome P450-Endogenous Substrate Metabolism
    David J Waxman; Fiscal Year: 2010
    ..Genome-wide approaches will be used to elucidate ..
  5. Cytochrome P450-Endogenous Substrate Metabolism
    David Waxman; Fiscal Year: 2006
    ..abstract_text> ..
  6. Cytochrome P450-Endogenous Substrate Metabolism
    David Waxman; Fiscal Year: 2007
    ..abstract_text> ..
  7. Hepatic Metabolism of Anti-cancer Drugs
    David Waxman; Fiscal Year: 2007
    ..abstract_text> ..
  8. Combination Therapies for Cancer Treatment
    David J Waxman; Fiscal Year: 2010
    ..These studies may identify strategies that can be used to improve the therapeutic effectiveness of anti-angiogenic drugs in the clinic, and thereby advance the development of anti-cancer therapies. ..
  9. Cytochrome P450-Endogenous Substrate Metabolism
    David Waxman; Fiscal Year: 2009
    ..Genome-wide approaches will be used to elucidate ..
  10. Combination Therapies for Cancer Treatment
    David J Waxman; Fiscal Year: 2011
    ..These studies may identify strategies that can be used to improve the therapeutic effectiveness of anti-angiogenic drugs in the clinic, and thereby advance the development of anti-cancer therapies. ..
  11. NADPH P450 REDUCTASE--THYROID HORMONE REGULATION
    David Waxman; Fiscal Year: 2002
    ....
  12. CYTOCHROME P450--ENDOGENOUS SUBSTRATE METABOLISM
    David Waxman; Fiscal Year: 2002
    ..abstract_text> ..
  13. Cytochrome P450-Endogenous Substrate Metabolism
    David Waxman; Fiscal Year: 2003
    ..abstract_text> ..
  14. HEPATIC METABOLISM OF ANTICANCER DRUGS
    David Waxman; Fiscal Year: 2003
    ..abstract_text> ..
  15. Hepatic Metabolism of Anti-cancer Drugs
    David Waxman; Fiscal Year: 2004
    ..cyclophosphamide and other P450 prodrugs in a manner that moderates toxic host responses and improves therapeutic effects, and will thereby advance the development and implementation of P450 prodrug-based therapies for cancer treatment ..