Research Topics
Species | David WaxmanSummaryAffiliation: Boston University Country: USA Publications
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Publications
Male-specific hepatic Bcl6: growth hormone-induced block of transcription elongation in females and binding to target genes inversely coordinated with STAT5Rosana D Meyer
Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, Massachusetts 02215, USA
Mol Endocrinol 23:1914-26. 2009..Bcl6 and STAT5 binding are thus inversely coordinated by the endogenous pulses of pituitary GH release, suggesting this male-specific transcriptional repressor modulates hepatic GH signaling to select STAT5 target genes...
Impact of tumor vascularity on responsiveness to antiangiogenesis in a prostate cancer stem cell-derived tumor modelKexiong Zhang
Corresponding Author David J Waxman, Department of Biology, 5 Cummington Mall, Boston, MA 02215
Mol Cancer Ther 12:787-98. 2013..Mol Cancer Ther; 12(5); 787-98. ©2013 AACR...- Impact of tumor blood flow modulation on tumor sensitivity to the bioreductive drug banoxantroneEugene Manley
Department of Biology, Boston University, 5 Cummington Street, Boston, MA 02215
J Pharmacol Exp Ther 344:368-77. 2013..Moreover, the activation of AQ4N cytotoxicity in vivo requires tumor hypoxia that is more extensive or prolonged than can readily be achieved by vasodilation or by antiangiogenic drug treatment... - Sex-specific mouse liver gene expression: genome-wide analysis of developmental changes from pre-pubertal period to young adulthoodTara L Conforto
Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, MA 02215, USA
Biol Sex Differ 3:9. 2012..abstract:.. - MAnorm: a robust model for quantitative comparison of ChIP-Seq data setsZhen Shao
Department of Pediatric Oncology, Dana Farber Cancer Institute, Boston, MA 02115, USA
Genome Biol 13:R16. 2012..The quantitative binding differences inferred by MAnorm showed strong correlation with both the changes in expression of target genes and the binding of cell type-specific regulators... - Dynamic, sex-differential STAT5 and BCL6 binding to sex-biased, growth hormone-regulated genes in adult mouse liverYijing Zhang
Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, Massachusetts, USA
Mol Cell Biol 32:880-96. 2012..The approaches used here to characterize sex-dependent STAT5 and BCL6 binding can be applied to other condition-specific regulatory factors and binding sites and their interplay with cooperative chromatin binding factors... - Complex modulation of androgen responsive gene expression by methoxyacetic acidGargi Bagchi
Department of Biology, Boston University, Boston, MA 02215, USA
Reprod Biol Endocrinol 9:42. 2011.... - Impact of methoxyacetic acid on mouse Leydig cell gene expressionGargi Bagchi
Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, MA 02215, USA
Reprod Biol Endocrinol 8:65. 2010..This study investigates the impact of MAA on gene expression in testicular Leydig cells, which play a critical role in germ cell survival and male reproductive function... - PC3 prostate tumor-initiating cells with molecular profile FAM65Bhigh/MFI2low/LEF1low increase tumor angiogenesisKexiong Zhang
Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, MA 02215, USA
Mol Cancer 9:319. 2010.... - Adenoviral delivery of pan-caspase inhibitor p35 enhances bystander killing by P450 gene-directed enzyme prodrug therapy using cyclophosphamide+Joshua C Doloff
Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, MA 02215, USA
BMC Cancer 10:487. 2010..Previous studies have shown that the pan-caspase inhibitor p35 significantly increases CPA-induced bystander killing by tumor cells that stably express P450 enzyme CYP2B6 (Schwartz et al, (2002) Cancer Res. 62: 6928-37)... - Sex differences in the expression of hepatic drug metabolizing enzymesDavid J Waxman
Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, MA 02215, USA
Mol Pharmacol 76:215-28. 2009.... 
Growth hormone regulation of sex-dependent liver gene expressionDavid J Waxman
Department of Biology, Boston University, 5 Cummington Street, Boston, Massachusetts 02215, USA
Mol Endocrinol 20:2613-29. 2006....- Harnessing apoptosis for improved anticancer gene therapyDavid J Waxman
Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, Massachusetts 02215, USA
Cancer Res 63:8563-72. 2003.... - Identification of novel enzyme-prodrug combinations for use in cytochrome P450-based gene therapy for cancerAlex Baldwin
Division of Cell and Molecular Biology, Department of Biology, Boston University, 5 Cummington St, MA 02215, USA
Arch Biochem Biophys 409:197-206. 2003.... - Collaboration between hepatic and intratumoral prodrug activation in a P450 prodrug-activation gene therapy model for cancer treatmentJie Ma
Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, MA 02215, USA
Mol Cancer Ther 6:2879-90. 2007.... - Sustained P450 expression and prodrug activation in bolus cyclophosphamide-treated cultured tumor cells. Impact of prodrug schedule on P450 gene-directed enzyme prodrug therapyPamela S Schwartz
Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, Massachussetts 02215, USA
Cancer Gene Ther 10:571-82. 2003.... - Use of replication-conditional adenovirus as a helper system to enhance delivery of P450 prodrug-activation genes for cancer therapyYoussef Jounaidi
Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, Massachusetts 02215, USA
Cancer Res 64:292-303. 2004.... - Role of hepatocyte nuclear factors in growth hormone-regulated, sexually dimorphic expression of liver cytochromes P450Christopher A Wiwi
Division of Cell and Molecular Biology, Department of Biology, Boston University, 5 Cummington Street, Boston, MA 02215, USA
Growth Factors 22:79-88. 2004.... - Role of hepatocyte nuclear factors in transcriptional regulation of male-specific CYP2A2Christopher A Wiwi
Division of Cell and Molecular Biology, Department of Biology Boston University, Boston, Massachusetts 02215, USA
J Biol Chem 280:3259-68. 2005.... - Activation of the anticancer prodrugs cyclophosphamide and ifosfamide: identification of cytochrome P450 2B enzymes and site-specific mutants with improved enzyme kineticsChong-Sheng Chen
Department of Biology, Boston University, Boston, MA 02215, USA
Mol Pharmacol 65:1278-85. 2004.... - Environmental phthalate monoesters activate pregnane X receptor-mediated transcriptionChristopher H Hurst
Division of Cell and Molecular Biology, Department of Biology, Boston University, MA 02215, USA
Toxicol Appl Pharmacol 199:266-74. 2004.... - Conditionally replicating adenoviruses for cancer treatmentYoussef Jounaidi
Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, MA 02215, USA
Curr Cancer Drug Targets 7:285-301. 2007..The continued expansion of the knowledge base of adenovirus biology will likely lead to further improvements in the design of the ideal oncolytic adenoviruses for cancer treatment... - Enantioselective metabolism and cytotoxicity of R-ifosfamide and S-ifosfamide by tumor cell-expressed cytochromes P450Chong-Sheng Chen
Division of Cell and Molocular Biology, Department of Biology, Boston University, Boston, MA 02215, USA
Drug Metab Dispos 33:1261-7. 2005.... - Enhanced antitumor activity of P450 prodrug-based gene therapy using the low Km cyclophosphamide 4-hydroxylase P450 2B11Youssef Jounaidi
Division of Cell and Molecular Biology, Department of Biology, Boston University, 5 Cummington Street, Boston, MA 02215, USA
Mol Cancer Ther 5:541-55. 2006..Thus, P450 gene-directed enzyme prodrug therapy can be greatly improved by using the low Km P450 enzyme 2B11, which catalyzes intratumoral activation of cyclophosphamide and ifosfamide at pharmacologically relevant drug concentrations... - Simultaneous, bidirectional inhibitory crosstalk between PPAR and STAT5bJonathan M Shipley
Division of Cell and Molecular Biology, Department of Biology, Boston University, MA 02215, USA
Toxicol Appl Pharmacol 199:275-84. 2004..Conversely, STAT5-activating hormones and cytokines may modulate the responsiveness of PPARs to their foreign chemical ligands... - Enhanced bystander cytotoxicity of P450 gene-directed enzyme prodrug therapy by expression of the antiapoptotic factor p35Pamela S Schwartz
Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, Massachusetts 02215, USA
Cancer Res 62:6928-37. 2002.... - Environmental and endogenous peroxisome proliferator-activated receptor gamma agonists induce bone marrow B cell growth arrest and apoptosis: interactions between mono(2-ethylhexyl)phthalate, 9-cis-retinoic acid, and 15-deoxy-Delta12,14-prostaglandin J2Jennifer J Schlezinger
Department of Environmental Health, Boston University School of Public Health, Boston, MA 02118, USA
J Immunol 173:3165-77. 2004..These data demonstrate that environmental phthalates can cooperate with an endogenous ligand, 15d-PGJ(2), to inhibit proliferation of and induce apoptosis in developing bone marrow B cells, potentially via PPARgamma activation... - Sexually dimorphic P450 gene expression in liver-specific hepatocyte nuclear factor 4alpha-deficient miceChristopher A Wiwi
Department of Biology, Boston University, 5 Cummington Street, Boston, Massachusetts 02215, USA
Mol Endocrinol 18:1975-87. 2004.... - Sex-specific early growth hormone response genes in rat liverValerie Wauthier
Department of Biology, Boston University, 5 Cummington Street, Boston, Massachusetts 02215, USA
Mol Endocrinol 22:1962-74. 2008..We conclude that GH acts via both positive and negative regulatory mechanisms to establish and maintain the sex specificity of liver gene expression... - Activation of PPARalpha and PPARgamma by environmental phthalate monoestersChristopher H Hurst
Department of Biology, Division of Cell and Molecular Biology, Boston University, Boston, Massachusetts 02215, USA
Toxicol Sci 74:297-308. 2003.... - Sexual dimorphism of rat liver gene expression: regulatory role of growth hormone revealed by deoxyribonucleic Acid microarray analysisAmrita Ahluwalia
Division of Cell and Molecular Biology, Department of Biology, Boston University, 5 Cummington Street, Boston, Massachusetts 02215, USA
Mol Endocrinol 18:747-60. 2004..Taken together, these studies establish that GH-regulated gene expression is the major mechanistic determinant of sexually dimorphic gene expression in the rat liver model... - Elevated basal expression of liver peroxisomal beta-oxidation enzymes and CYP4A microsomal fatty acid omega-hydroxylase in STAT5b(-/-) mice: cross-talk in vivo between peroxisome proliferator-activated receptor and signal transducer and activator of transYuan Chun Zhou
Department of Biology, Division of Cell and Molecular Biology, Boston University, Boston, Massachusetts 02215, USA
Toxicol Appl Pharmacol 182:1-10. 2002.... - Characterization of three growth hormone-responsive transcription factors preferentially expressed in adult female liverEkaterina V Laz
Department of Biology, Boston University, 5 Cummington Street, Boston, Massachusetts 02215, USA
Endocrinology 148:3327-37. 2007..Binding sites for Cutl1, whose DNA-binding specificity is close to that of Cutl2, were statistically overrepresented in STAT5b-dependent male-specific mouse genes, lending support to this hypothesis... - Cytochrome p450-based gene therapies for cancerE Antonio Chiocca
Molecular Neuro-Oncology Laboratory, Neurosurgery Service, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA
Methods Mol Med 90:203-22. 2004 - Unbiased, genome-wide in vivo mapping of transcriptional regulatory elements reveals sex differences in chromatin structure associated with sex-specific liver gene expressionGuoyu Ling
Department of Biology, Boston University, 5 Cummington Street, Boston, MA 02215, USA
Mol Cell Biol 30:5531-44. 2010..This approach can readily be applied to mapping condition-specific regulatory sites in mammalian tissues under a wide variety of physiological conditions... - Down-regulation of STAT5b transcriptional activity by ligand-activated peroxisome proliferator-activated receptor (PPAR) alpha and PPARgammaJonathan M Shipley
Department of Biology, Boston University, 5 Cummington St, Boston, MA 02215, USA
Mol Pharmacol 64:355-64. 2003..These findings demonstrate the bidirectionality of cross-talk between the PPAR and STAT pathways and provide a mechanism whereby exposure to environmental chemical activators of PPAR can suppress expression of GH target genes... - Modulation of the antitumor activity of metronomic cyclophosphamide by the angiogenesis inhibitor axitinibJie Ma
Division of Cell and Molecular Biology, Department of Biology, Boston University, 5 Cummington Street, Boston, MA 02215, USA
Mol Cancer Ther 7:79-89. 2008.... - Role of STAT5a in regulation of sex-specific gene expression in female but not male mouse liver revealed by microarray analysisKarl H Clodfelter
Division of Cell and Molecular Biology, Department of Biology, Boston University, Massachusetts, USA
Physiol Genomics 31:63-74. 2007..These findings highlight the importance of STAT5a for regulation of sex-specific gene expression specifically in female liver, in striking contrast to STAT5b, whose major effects are restricted to male liver... - Loss of sexually dimorphic liver gene expression upon hepatocyte-specific deletion of Stat5a-Stat5b locusMinita G Holloway
Department of Biology, Boston University, Boston, Massachusetts 02215, USA
Endocrinology 148:1977-86. 2007..Thus, the major loss of liver sexual dimorphism in hepatocyte STAT5ab-deficient mice can primarily be attributed to the loss of STAT5b... - Sexual dimorphism of rat liver nuclear proteins: regulatory role of growth hormoneEkaterina V Laz
Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, MA 02215, USA
Mol Cell Proteomics 3:1170-80. 2004.... - Signalling cross-talk between hepatocyte nuclear factor 4alpha and growth-hormone-activated STAT5bSoo Hee Park
Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, MA 02215, USA
Biochem J 397:159-68. 2006.... - Sex-dependent liver gene expression is extensive and largely dependent upon signal transducer and activator of transcription 5b (STAT5b): STAT5b-dependent activation of male genes and repression of female genes revealed by microarray analysisKarl H Clodfelter
Department of Biology, Boston University, 5 Cummington Street, Boston, Massachusetts 02215, USA
Mol Endocrinol 20:1333-51. 2006..Several female-predominant repressors are elevated in STAT5b-deficient males; these may contribute to the major loss of male gene expression seen in the absence of STAT5b... - Codependence of growth hormone-responsive, sexually dimorphic hepatic gene expression on signal transducer and activator of transcription 5b and hepatic nuclear factor 4alphaMinita G Holloway
Division of Cell and Molecular Biology, Department of Biology, Boston University, 5 Cummington Street, Boston, Massachusetts 02215, USA
Mol Endocrinol 20:647-60. 2006.... - Impact of dimethyl sulfoxide on expression of nuclear receptors and drug-inducible cytochromes P450 in primary rat hepatocytesTing Su
Department of Biology, Division of Cell and Molecular Biology, Boston University, 5 Cummington Street, Boston, MA 02215, USA
Arch Biochem Biophys 424:226-34. 2004.... - Use of 7-ethoxycoumarin to monitor multiple enzymes in the human CYP1, CYP2, and CYP3 familiesDavid J Waxman
Department of Biology, Boston University, MA, USA
Methods Mol Biol 320:153-6. 2006.... - Combination of antiangiogenesis with chemotherapy for more effective cancer treatmentJie Ma
Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, MA 02215, USA
Mol Cancer Ther 7:3670-84. 2008..New targets for antiangiogenesis continue to be discovered, increasing the opportunities to interdict tumor angiogenesis and circumvent resistance mechanisms that may emerge with chronic use of these drugs... - Dynamic in vivo binding of STAT5 to growth hormone-regulated genes in intact rat liver. Sex-specific binding at low- but not high-affinity STAT5 sitesEkaterina V Laz
Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, Massachusetts 02215, USA
Mol Endocrinol 23:1242-54. 2009.... - Intrinsic sex differences in the early growth hormone responsiveness of sex-specific genes in mouse liverValerie Wauthier
Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, Massachusetts 02215, USA
Mol Endocrinol 24:667-78. 2010.... - Computational solvent mapping reveals the importance of local conformational changes for broad substrate specificity in mammalian cytochromes P450Karl H Clodfelter
Bioinformatics Program, Boston University, 44 Cummington Street, Boston, Massachusetts 02215, USA
Biochemistry 45:9393-407. 2006..In addition, the binding of S-warfarin to P450 2C9 creates a high-affinity site for a second ligand, which may help to explain the prevalence of drug-drug interactions involving this and other mammalian P450s... - Antitumor activity of methoxymorpholinyl doxorubicin: potentiation by cytochrome P450 3A metabolismHong Lu
Department of Biology, Boston University, 5 Cummington Street, Boston, MA 02215, USA
Mol Pharmacol 67:212-9. 2005.... - Sexual dimorphism of hepatic gene expression: novel biological role of KRAB zinc finger repressors revealedDavid J Waxman
Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, Massachusetts 02215, USA
Genes Dev 17:2607-13. 2003 - Spectrofluorometric analysis of CYP2A6-catalyzed coumarin 7-hydroxylationDavid J Waxman
Department of Biology, Boston University, MA, USA
Methods Mol Biol 320:91-6. 2006.... - Aryl hydrocarbon receptor-independent activation of estrogen receptor-dependent transcription by 3-methylcholanthreneJonathan M Shipley
Division of Cell and Molecular Biology, Department of Biology, Boston University, 5 Cummington Street, MA 02215, USA
Toxicol Appl Pharmacol 213:87-97. 2006..3MC may thus elicit estrogenic activity by multiple mechanisms... - Dominant effect of antiangiogenesis in combination therapy involving cyclophosphamide and axitinibJie Ma
Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, Massachusetts 02215, USA
Clin Cancer Res 15:578-88. 2009..Furthermore, the effect of axitinib on the antitumor activity of combination treatments with cyclophosphamide was examined... - The structural basis of pregnane X receptor binding promiscuityChi Ho Ngan
Department of Biomedical Engineering, Boston University, Boston, Massachusetts 02215, USA
Biochemistry 48:11572-81. 2009..Results also suggest a unique signal transduction mechanism between the PXR homodimerization interface and its coactivator binding site... - Interactions of methoxyacetic acid with androgen receptorGargi Bagchi
Division of Cell and Molecular Biology, Department of Biology, Boston University, 5 Cummington Street, Boston, MA 02215, USA
Toxicol Appl Pharmacol 238:101-10. 2009..Deregulation of these genes may alter androgen synthesis and action in a manner that contributes to MAA-induced testicular toxicity... - Liver-specific hepatocyte nuclear factor-4alpha deficiency: greater impact on gene expression in male than in female mouse liverMinita G Holloway
Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, Massachusetts 02215, USA
Mol Endocrinol 22:1274-86. 2008..Thus, a substantial fraction of the HNF4alpha-dependent genes reported here are likely to be direct targets of HNF4alpha... - Role of the cytokine-induced SH2 domain-containing protein CIS in growth hormone receptor internalizationTanya Landsman
Division of Cell and Molecular Biology, Department of Biology, Boston University, MA 02215, USA
J Biol Chem 280:37471-80. 2005.... - Human telomerase reverse transcriptase promoter-driven oncolytic adenovirus with E1B-19 kDa and E1B-55 kDa gene deletionsJoshua C Doloff
Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, MA 02215, USA
Hum Gene Ther 19:1383-400. 2008..Adeno-hTERT-E1A thus has strong therapeutic potential and an improved safety profile compared with ONYX-015, which may lead to reduced toxicity in the clinic... - Exploring the binding site structure of the PPAR gamma ligand-binding domain by computational solvent mappingShu-Hsien Sheu
Department of Biomedical Engineering, Boston University, 44 Cummington Street, Boston, Massachusetts 02215, USA
Biochemistry 44:1193-209. 2005..The number of probe clusters retained by these sites depends on the properties of the bound agonist, providing information on the origin of correlations between ligand and coactivator binding... - Post-transcriptional regulation of hepatic NADPH-cytochrome P450 reductase by thyroid hormone: independent effects on poly(A) tail length and mRNA stabilityDongxu Liu
Division of Cell and Molecular Biology, Department of Biology, Boston University, Boston, Massachusetts, USA
Mol Pharmacol 61:1089-96. 2002.... - trans-activation of PPARalpha and induction of PPARalpha target genes by perfluorooctane-based chemicalsJonathan M Shipley
Division of Cell and Molecular Biology, Department of Biology, Boston University, 5 Cummington Street, Boston, Massachusetts 02215, USA
Toxicol Sci 80:151-60. 2004.... - Evaluation of thyroid hormone effects on liver P450 reductase translationEkaterina V Apletalina
Department of Biology, Boston University, 5 Cummington Street, MA 02215, USA
Arch Biochem Biophys 409:172-9. 2003..Taken together, these data suggest that P450R may in part be regulated at the level of protein stability in hyperthyroid rat liver... - Computational screening of phthalate monoesters for binding to PPARgammaTaner Kaya
Department of Chemistry, Boston University, Boston, Massachusetts 02215, USA
Chem Res Toxicol 19:999-1009. 2006..These findings support the use of computational methods to identify environmental chemicals that warrant further experimental evaluation for PPAR binding and trans-activation potential in cell-based models... - Cytochrome P450 gene-directed enzyme prodrug therapy (GDEPT) for cancerLing Chen
Department of Gene Transfer Technologies and Therapeutics, Lexicon Genetics Inc, The Woodlands, TX 77381, USA
Curr Pharm Des 8:1405-16. 2002..A recent report of clinical efficacy in a P450-based phase I/II gene therapy trial for pancreatic cancer patients supports this conclusion... - Growth hormone determines sexual dimorphism of hepatic cytochrome P450 3A4 expression in transgenic miceConnie Cheung
Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Building 37, Room 3106, Bethesda, MD 20892, USA
J Pharmacol Exp Ther 316:1328-34. 2006.... - Activation of oxazaphosphorines by cytochrome P450: application to gene-directed enzyme prodrug therapy for cancerPartha Roy
Forest Research Institute, A Division of Forest Laboratories, Inc, Harborside Financial Center, Plaza V, Jersey City, NJ 07311, USA
Toxicol In Vitro 20:176-86. 2006..Recent advances in this area of research are reviewed, and two proof-of-concept clinical trials that highlight the utility of this strategy are discussed... - A mouse model with liver-specific deletion and global suppression of the NADPH-cytochrome P450 reductase gene: characterization and utility for in vivo studies of cyclophosphamide dispositionJun Gu
Wadsworth Center, New York State Department of Health, Empire State Plaza, Box 509, Albany, NY 12201 0509, USA
J Pharmacol Exp Ther 321:9-17. 2007.... - Directed evolution of mammalian cytochrome P450 2B1: mutations outside of the active site enhance the metabolism of several substrates, including the anticancer prodrugs cyclophosphamide and ifosfamideSantosh Kumar
Department of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston, 77555, USA
J Biol Chem 280:19569-75. 2005..5-fold enhancement with ifosfamide. Directed evolution can thus be used to enhance P450 2B1 catalytic efficiency across a panel of substrates and to identify functionally important residues distant from the active site... - Re-engineering cytochrome P450 2B11dH for enhanced metabolism of several substrates including the anti-cancer prodrugs cyclophosphamide and ifosfamideLing Sun
Department of Pharmacology and Toxicology, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555 1031, USA
Arch Biochem Biophys 458:167-74. 2007..Overall, the re-engineered P450 2B11dH enzymes exhibited enhanced catalytic efficiency with several substrates including the anti-cancer prodrugs... - Synthetic drugs and natural products as modulators of constitutive androstane receptor (CAR) and pregnane X receptor (PXR)Thomas K H Chang
Faculty of Pharmaceutical Sciences, The University of British Columbia, Vancouver, Canada
Drug Metab Rev 38:51-73. 2006..This article discusses the importance of these receptors for pharmacology and toxicology, emphasizing the role of individual drugs and natural products as agonists, indirect activators, inverse agonists, and antagonists of CAR and PXR... - Cancer chemotherapy and drug metabolismDavid S Riddick
Department of Pharmacology, Medical Sciences Building, University of Toronto, Toronto, Ontario, Canada
Drug Metab Dispos 33:1083-96. 2005..A clear theme emerged from this symposium: drug metabolism and transport processes can be modulated and exploited in ways that may offer distinct therapeutic advantages in the management of patients with cancer... - Pregnane X receptor-mediated transcriptionThomas K H Chang
Faculty of Pharmaceutical Sciences, The University of British Columbia, Vancouver, Canada
Methods Enzymol 400:588-98. 2005.... - Enzymatic analysis of cDNA-expressed human CYP1A1, CYP1A2, and CYP1B1 with 7-ethoxyresorufin as substrateThomas K H Chang
Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, Canada
Methods Mol Biol 320:85-90. 2006..It can also be used to assay 7-ethoxyresorufin O-dealkylation activity in isolated hepatocytes and cultured cells that express this P450 activity... - Determination of CYP2B6 component of 7-ethoxy-4-trifluoromethylcoumarin O-deethylation activity in human liver microsomesThomas K H Chang
Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, Canada
Methods Mol Biol 320:97-102. 2006..This approach can be modified to assay the catalytic activity of cDNA-expressed CYP2B6... - High-performance liquid chromatography analysis of CYP2C8-catalyzed paclitaxel 6alpha-hydroxylationCharles L Crespi
Discovery Labware, BD Biosciences, Woburn, MA, USA
Methods Mol Biol 320:103-7. 2006..This method is applicable to enzymatic studies for determination of CYP2C8-catalyzed paclitaxel 6alpha-hydroxylation activity... - Determination of CYP2C9-catalyzed diclofenac 4'-hydroxylation by high-performance liquid chromatographyCharles L Crespi
Discovery Labware, BD Biosciences, Woburn, MA, USA
Methods Mol Biol 320:109-13. 2006..This method is applicable to enzymatic studies for determination of CYP2C9-catalyzed diclofenac 4'-hydroxylation activity... - CYP2C19-mediated (S)-mephenytoin 4'-hydroxylation assayed by high-performance liquid chromatography with radiometric detectionCharles L Crespi
Discovery Labware, BD Biosciences, Woburn, MA, USA
Methods Mol Biol 320:115-9. 2006..This method is applicable to enzymatic studies for determination of CYP2C19-catalyzed (S)-mephenytoin 4'-hydroxylation activity... - Catalytic assays for human cytochrome P450: an introductionThomas K H Chang
Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, Canada
Methods Mol Biol 320:73-83. 2006..Cautions that need to be exercised when using these substrates to probe for individual P450 activities in human liver and other tissues are discussed... - Spectrophotometric analysis of human CYP2E1-catalyzed p-nitrophenol hydroxylationThomas K H Chang
Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, Canada
Methods Mol Biol 320:127-31. 2006..This method is applicable to enzymatic studies for determination of P450-catalyzed p-nitrophenol hydroxylation activity... - Determination of CYP4A11-catalyzed lauric acid 12-hydroxylation by high-performance liquid chromatography with radiometric detectionCharles L Crespi
Discovery Labware, BD Biosciences, Woburn, MA, USA
Methods Mol Biol 320:143-7. 2006..This method is applicable to enzymatic studies for determination of lauric acid 12-hydroxylation activity...
Research Grants
- Hepatic Metabolism of Anti-cancer DrugsDavid Waxman; Fiscal Year: 2007..abstract_text> ..
- Cytochrome P450-Endogenous Substrate MetabolismDavid J Waxman; Fiscal Year: 2010....
- Cytochrome P450-Endogenous Substrate MetabolismDavid J Waxman; Fiscal Year: 2010....
- Hepatic Metabolism of Anti-cancer DrugsDavid Waxman; Fiscal Year: 2004..cyclophosphamide and other P450 prodrugs in a manner that moderates toxic host responses and improves therapeutic effects, and will thereby advance the development and implementation of P450 prodrug-based therapies for cancer treatment ..
- Cytochrome P450-Endogenous Substrate MetabolismDavid Waxman; Fiscal Year: 2006..abstract_text> ..
- Cytochrome P450-Endogenous Substrate MetabolismDavid Waxman; Fiscal Year: 2007..abstract_text> ..
- Hepatic Metabolism of Anti-cancer DrugsDavid Waxman; Fiscal Year: 2007..abstract_text> ..
- Combination Therapies for Cancer TreatmentDavid J Waxman; Fiscal Year: 2010..These studies may identify strategies that can be used to improve the therapeutic effectiveness of anti-angiogenic drugs in the clinic, and thereby advance the development of anti-cancer therapies. ..
- Cytochrome P450-Endogenous Substrate MetabolismDavid Waxman; Fiscal Year: 2009..Genome-wide approaches will be used to elucidate ..
- Combination Therapies for Cancer TreatmentDavid J Waxman; Fiscal Year: 2011..These studies may identify strategies that can be used to improve the therapeutic effectiveness of anti-angiogenic drugs in the clinic, and thereby advance the development of anti-cancer therapies. ..
- NADPH P450 REDUCTASE--THYROID HORMONE REGULATIONDavid Waxman; Fiscal Year: 2002....
- CYTOCHROME P450--ENDOGENOUS SUBSTRATE METABOLISMDavid Waxman; Fiscal Year: 2002..abstract_text> ..
- Cytochrome P450-Endogenous Substrate MetabolismDavid Waxman; Fiscal Year: 2003..abstract_text> ..
- HEPATIC METABOLISM OF ANTICANCER DRUGSDavid Waxman; Fiscal Year: 2003..abstract_text> ..
- Cytochrome P450-Endogenous Substrate MetabolismDavid J Waxman; Fiscal Year: 2010..Genome-wide approaches will be used to elucidate ..