John C Samuelson

Summary

Affiliation: Boston University
Country: USA

Publications

  1. pmc Trichomonas transmembrane cyclases result from massive gene duplication and concomitant development of pseudogenes
    Jike Cui
    Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston, Massachusetts, USA
    PLoS Negl Trop Dis 4:e782. 2010
  2. pmc Evidence for a "wattle and daub" model of the cyst wall of entamoeba
    Anirban Chatterjee
    Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston, MA, USA
    PLoS Pathog 5:e1000498. 2009
  3. pmc The Jacob2 lectin of the Entamoeba histolytica cyst wall binds chitin and is polymorphic
    Sudip K Ghosh
    Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston, Massachusetts, USA
    PLoS Negl Trop Dis 4:e750. 2010
  4. pmc Evidence for a structural role for acid-fast lipids in oocyst walls of Cryptosporidium, Toxoplasma, and Eimeria
    G Guy Bushkin
    Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston, Massachusetts, USA
    MBio 4:e00387-13. 2013
  5. pmc Giardia cyst wall protein 1 is a lectin that binds to curled fibrils of the GalNAc homopolymer
    Aparajita Chatterjee
    Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston, Massachusetts, USA
    PLoS Pathog 6:e1001059. 2010
  6. pmc A simple fibril and lectin model for cyst walls of Entamoeba and perhaps Giardia
    John Samuelson
    Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston, MA, USA
    Trends Parasitol 27:17-22. 2011
  7. pmc The diversity of dolichol-linked precursors to Asn-linked glycans likely results from secondary loss of sets of glycosyltransferases
    John Samuelson
    Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, 715 Albany Street, Boston, MA 02118 2932, USA
    Proc Natl Acad Sci U S A 102:1548-53. 2005
  8. ncbi request reprint Gene expansion in Trichomonas vaginalis: a case study on transmembrane cyclases
    Jike Cui
    Boston University, Boston, MA 02215, U S A
    Genome Inform 18:35-43. 2007
  9. pmc A spliceosomal intron in Giardia lamblia
    Julie E J Nixon
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 02115 6018, USA
    Proc Natl Acad Sci U S A 99:3701-5. 2002
  10. pmc Heterologous expression of an Entamoeba histolytica chitin synthase in Saccharomyces cerevisiae
    Katrina L Van Dellen
    Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, 715 Albany St, Evans 426, Boston, Massachusetts 02118, USA
    Eukaryot Cell 5:203-6. 2006

Collaborators

  • Temple Smith
  • Jike Cui
  • Hilary G Morrison
  • Brendan Loftus
  • M L Sogin
  • Jitender P Dubey
  • Reid Gilmore
  • Paula Magnelli
  • Phillips W Robbins
  • Daniel M Ratner
  • Sulagna Banerjee
  • G Guy Bushkin
  • Katrina L Van Dellen
  • Sudip K Ghosh
  • Kariona A Grabińska
  • Anirban Chatterjee
  • Aparajita Chatterjee
  • Martin Steffen
  • Julie E J Nixon
  • Catherine E Costello
  • Andrea Carpentieri
  • Esther Bullitt
  • Ziqiang Guan
  • Christian R H Raetz
  • Suchismita Das
  • Luis M Bredeston
  • Amy Wang
  • Andrew G McArthur
  • Edwin Motari
  • Jeffrey D Dvorin
  • Rashidul Haque
  • Manoj T Duraisingh
  • Seth D Robertson
  • Tuli Dey
  • Cameron V Jennings
  • Barry R O'Keefe
  • Marc Jan Gubbels
  • Ken Jang
  • Landon Moore
  • Landon L Moore
  • Daniel J Kelleher
  • Prashanth Vishwanath
  • Dorota Bulik
  • Charles A Specht
  • James Head
  • John F Cipollo
  • Dorota A Bulik
  • Katrina Van Dellen
  • Carlos B Hirschberg
  • Carolina E Caffaro
  • Jessica Field

Detail Information

Publications23

  1. pmc Trichomonas transmembrane cyclases result from massive gene duplication and concomitant development of pseudogenes
    Jike Cui
    Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston, Massachusetts, USA
    PLoS Negl Trop Dis 4:e782. 2010
    ..With the goal of beginning to understand why some Trichomonas genes are present in so many copies, we characterized here a family of approximately 123 Trichomonas genes that encode transmembrane adenylyl cyclases (TMACs)...
  2. pmc Evidence for a "wattle and daub" model of the cyst wall of entamoeba
    Anirban Chatterjee
    Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston, MA, USA
    PLoS Pathog 5:e1000498. 2009
    ....
  3. pmc The Jacob2 lectin of the Entamoeba histolytica cyst wall binds chitin and is polymorphic
    Sudip K Ghosh
    Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston, Massachusetts, USA
    PLoS Negl Trop Dis 4:e750. 2010
    ..The goal here was to determine how well the Ei model applies to Entamoeba cysts from humans...
  4. pmc Evidence for a structural role for acid-fast lipids in oocyst walls of Cryptosporidium, Toxoplasma, and Eimeria
    G Guy Bushkin
    Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston, Massachusetts, USA
    MBio 4:e00387-13. 2013
    ..We propose a two-layered model of the oocyst wall (glucan and acid-fast lipids) that resembles the two-layered walls of mycobacteria (peptidoglycan and acid-fast lipids) and plants (cellulose and cutin)...
  5. pmc Giardia cyst wall protein 1 is a lectin that binds to curled fibrils of the GalNAc homopolymer
    Aparajita Chatterjee
    Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston, Massachusetts, USA
    PLoS Pathog 6:e1001059. 2010
    ..During excystation, host and Giardia proteases appear to degrade bound CWPs, exposing fibrils of the GalNAc homopolymer that are digested by a stage-specific glycohydrolase...
  6. pmc A simple fibril and lectin model for cyst walls of Entamoeba and perhaps Giardia
    John Samuelson
    Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston, MA, USA
    Trends Parasitol 27:17-22. 2011
    ..Although many of the details remain to be determined for the cyst wall of Giardia, current data suggest a relatively simple fibril and lectin model for the Entamoeba cyst wall...
  7. pmc The diversity of dolichol-linked precursors to Asn-linked glycans likely results from secondary loss of sets of glycosyltransferases
    John Samuelson
    Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, 715 Albany Street, Boston, MA 02118 2932, USA
    Proc Natl Acad Sci U S A 102:1548-53. 2005
    ..Finally, the present diversity of protist and fungal dolichol-PP-linked glycans appears to result from secondary loss of glycosyltransferases from a common ancestor that contained the complete set of Alg glycosyltransferases...
  8. ncbi request reprint Gene expansion in Trichomonas vaginalis: a case study on transmembrane cyclases
    Jike Cui
    Boston University, Boston, MA 02215, U S A
    Genome Inform 18:35-43. 2007
    ..We gave three possible hypotheses for that observation: a) Sequencing error or stop-codon read-through; b) Recency of duplication and mutation; c) The likelihood of functional pseudogene...
  9. pmc A spliceosomal intron in Giardia lamblia
    Julie E J Nixon
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 02115 6018, USA
    Proc Natl Acad Sci U S A 99:3701-5. 2002
    ..If introns were added during eukaryotic evolution (the introns-late hypothesis), then these results push back the date of this event before the branching of G. lamblia...
  10. pmc Heterologous expression of an Entamoeba histolytica chitin synthase in Saccharomyces cerevisiae
    Katrina L Van Dellen
    Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, 715 Albany St, Evans 426, Boston, Massachusetts 02118, USA
    Eukaryot Cell 5:203-6. 2006
    ..EhCHS-1 is deposited chitin in the lateral wall of transformed Saccharomyces cerevisiae Chs mutants, independent of accessory proteins (Chs4p to Chs7p) required by yeast Chs3p...
  11. pmc Unique Asn-linked oligosaccharides of the human pathogen Entamoeba histolytica
    Paula Magnelli
    Department of Molecular and Cell Biology, Department of Biochemistry, Boston University Medical Center, Boston, MA 02118 2526, USA
    J Biol Chem 283:18355-64. 2008
    ..In summary, E. histolytica N-glycans include unprocessed Man(5)GlcNAc(2), which is a target for cyanovirin-N, as well as unique, complex N-glycans containing Gal and Glc...
  12. pmc Dolichyl-phosphate-glucose is used to make O-glycans on glycoproteins of Trichomonas vaginalis
    Kariona A Grabińska
    Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, 715 Albany Street, Evans 425, Boston, MA 02118, USA
    Eukaryot Cell 7:1344-51. 2008
    ..To our knowledge, this is the first demonstration of Dol-P-Glc as a sugar donor for O-glycans on glycoproteins...
  13. pmc Suggestive evidence for Darwinian Selection against asparagine-linked glycans of Plasmodium falciparum and Toxoplasma gondii
    G Guy Bushkin
    Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston, MA 02118, USA
    Eukaryot Cell 9:228-41. 2010
    ..In addition, occupation of N-glycan sites is markedly reduced in apicoplast proteins versus some secretory proteins in both Plasmodium and Toxoplasma...
  14. pmc Darwinian selection for sites of Asn-linked glycosylation in phylogenetically disparate eukaryotes and viruses
    Jike Cui
    Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston, MA 02118, USA
    Proc Natl Acad Sci U S A 106:13421-6. 2009
    ..We conclude that there is Darwinian selection for sequons in phylogenetically disparate eukaryotes and viruses...
  15. pmc The evolution of N-glycan-dependent endoplasmic reticulum quality control factors for glycoprotein folding and degradation
    Sulagna Banerjee
    Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston, MA 02118, USA
    Proc Natl Acad Sci U S A 104:11676-81. 2007
    ..We conclude that N-glycan precursor length has profound effects on N-glycan-dependent QC of glycoprotein folding and ERAD...
  16. pmc Changes in the N-glycome, glycoproteins with Asn-linked glycans, of Giardia lamblia with differentiation from trophozoites to cysts
    Daniel M Ratner
    Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, 715 Albany Street, Evans 425, Boston, MA 02118, USA
    Eukaryot Cell 7:1930-40. 2008
    ..In summary, there are major changes in the Giardia N-glycome with the differentiation from trophozoites to cysts...
  17. pmc Unique posttranslational modifications of chitin-binding lectins of Entamoeba invadens cyst walls
    Katrina L Van Dellen
    Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston, MA 02118, USA
    Eukaryot Cell 5:836-48. 2006
    ..invadens cyst wall all contain chitin-binding domains (chitinases, Jessie lectins, and Jacob lectins) and that the Jacob lectins are differentially modified by site-specific Cys proteases and O-phosphodiester-linked glycans...
  18. ncbi request reprint The cyst wall of Entamoeba invadens contains chitosan (deacetylated chitin)
    Suchismita Das
    Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, MA 02118, USA
    Mol Biochem Parasitol 148:86-92. 2006
    ..These results are consistent with the idea that Ei chitin deacetylases modify chitin to produce chitosan in the Ei cyst wall...
  19. doi request reprint Use of Giardia, which appears to have a single nucleotide-sugar transporter for UDP-GlcNAc, to identify the UDP-Glc transporter of Entamoeba
    Sulagna Banerjee
    Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston, MA 02118 2492, USA
    Mol Biochem Parasitol 159:44-53. 2008
    ..In summary, all three NSTs of Entamoeba and the single NST of Giardia have been molecularly characterized, and transfected Giardia provides a new system for testing heterologous UDP-Glc transporters...
  20. ncbi request reprint Golgi and endoplasmic reticulum functions take place in different subcellular compartments of Entamoeba histolytica
    Luis M Bredeston
    Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Massachusetts 02118 2492, USA
    J Biol Chem 280:32168-76. 2005
    ..Together, these studies demonstrate that E. histolytica has different vesicles that play a role in protein glycosylation and folding quality control, analogous to the above organellar functions of higher eukaryotes...
  21. pmc Evidence for lateral transfer of genes encoding ferredoxins, nitroreductases, NADH oxidase, and alcohol dehydrogenase 3 from anaerobic prokaryotes to Giardia lamblia and Entamoeba histolytica
    Julie E J Nixon
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    Eukaryot Cell 1:181-90. 2002
    ..In further support of lateral transfer, the G. lamblia NADH oxidase and adh3 genes appeared to have an evolutionary history distinct from those of E. histolytica...
  22. pmc Molecular characterization of the cis-prenyltransferase of Giardia lamblia
    Kariona A Grabińska
    Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston, MA 02118, USA
    Glycobiology 20:824-32. 2010
    ..These results suggest that the synthetic pathway for Dol-P is conserved in Giardia, even if some of the important enzymes are different from those of higher eukaryotes or remain unidentified...

Research Grants17

  1. Multiple Lectin Model of the Cyst Wall of Entamoeba
    John C Samuelson; Fiscal Year: 2010
    ..We are studying the cyst wall of Eh, because cysts are the infectious and diagnostic form. These studies will lead to a better understanding of how Eh causes disease and perhaps to better diagnostics. ..
  2. THREE LECTIN MODEL OF AMEBIC ENCYSTATION AND EXCYSTATION
    John Samuelson; Fiscal Year: 2002
    ..An encystations-specific chitinase had a Cys-richlectin domain, which resembles those of wheat germ agglutinin and plant chitinases. GlucNAc inhibited binding of Jacob and chitinase to chitin in SDS-treated cyst walls. ..
  3. Multiple Lectin Model of the Cyst Wall of Entamoeba
    John Samuelson; Fiscal Year: 2007
    ..We are studying the cyst wall of Eh, because cysts are the infectious and diagnostic form. These studies will lead to a better understanding of how Eh causes disease and perhaps to better diagnostics. ..
  4. Multiple Lectin Model of the Cyst Wall of Entamoeba
    John Samuelson; Fiscal Year: 2006
    ..We are studying the cyst wall of Eh, because cysts are the infectious and diagnostic form. These studies will lead to a better understanding of how Eh causes disease and perhaps to better diagnostics. ..
  5. Multiple Lectin Model of the Cyst Wall of Entamoeba
    John Samuelson; Fiscal Year: 2009
    ..We are studying the cyst wall of Eh, because cysts are the infectious and diagnostic form. These studies will lead to a better understanding of how Eh causes disease and perhaps to better diagnostics. ..
  6. Cell Biology, Genetics, and Biochemistry of Giardia
    John Samuelson; Fiscal Year: 2007
    ..Major hypothesis for Aim 3 (biochemistry): Giardia is sensitive to metronidazole and furazolidone, because of the presence of nitroreductases, like those of anaerobic bacteria, which reduce and activate these drugs. ..
  7. Cell Biology, Genetics, and Biochemistry of Giardia
    John Samuelson; Fiscal Year: 2006
    ..Major hypothesis for Aim 3 (biochemistry): Giardia is sensitive to metronidazole and furazolidone, because of the presence of nitroreductases, like those of anaerobic bacteria, which reduce and activate these drugs. ..
  8. THREE LECTIN MODEL OF AMEBIC ENCYSTATION AND EXCYSTATION
    John Samuelson; Fiscal Year: 2004
    ..An encystations-specific chitinase had a Cys-richlectin domain, which resembles those of wheat germ agglutinin and plant chitinases. GlucNAc inhibited binding of Jacob and chitinase to chitin in SDS-treated cyst walls. ..
  9. THREE LECTIN MODEL OF AMEBIC ENCYSTATION AND EXCYSTATION
    John Samuelson; Fiscal Year: 2002
    ..An encystations-specific chitinase had a Cys-richlectin domain, which resembles those of wheat germ agglutinin and plant chitinases. GlucNAc inhibited binding of Jacob and chitinase to chitin in SDS-treated cyst walls. ..
  10. THREE LECTIN MODEL OF AMEBIC ENCYSTATION AND EXCYSTATION
    John Samuelson; Fiscal Year: 2003
    ..An encystations-specific chitinase had a Cys-richlectin domain, which resembles those of wheat germ agglutinin and plant chitinases. GlucNAc inhibited binding of Jacob and chitinase to chitin in SDS-treated cyst walls. ..
  11. Cell Biology, Genetics, and Biochemistry of Giardia
    John Samuelson; Fiscal Year: 2003
    ..Major hypothesis for Aim 3 (biochemistry): Giardia is sensitive to metronidazole and furazolidone, because of the presence of nitroreductases, like those of anaerobic bacteria, which reduce and activate these drugs. ..
  12. THREE LECTIN MODEL OF AMEBIC ENCYSTATION AND EXCYSTATION
    John Samuelson; Fiscal Year: 2001
    ..An encystations-specific chitinase had a Cys-richlectin domain, which resembles those of wheat germ agglutinin and plant chitinases. GlucNAc inhibited binding of Jacob and chitinase to chitin in SDS-treated cyst walls. ..
  13. THREE LECTIN MODEL OF AMEBIC ENCYSTATION AND EXCYSTATION
    John Samuelson; Fiscal Year: 2001
    ..An encystations-specific chitinase had a Cys-richlectin domain, which resembles those of wheat germ agglutinin and plant chitinases. GlucNAc inhibited binding of Jacob and chitinase to chitin in SDS-treated cyst walls. ..
  14. THREE LECTIN MODEL OF AMEBIC ENCYSTATION AND EXCYSTATION
    John Samuelson; Fiscal Year: 2000
    ..An encystations-specific chitinase had a Cys-richlectin domain, which resembles those of wheat germ agglutinin and plant chitinases. GlucNAc inhibited binding of Jacob and chitinase to chitin in SDS-treated cyst walls. ..