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Genomes and Genes | Paul F PilchSummaryAffiliation: Boston University Country: USA Publications
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Publications
Pharmacological targeting of adipocytes/fat metabolism for treatment of obesity and diabetesPaul F Pilch
Department of Biochemistry, Boston University School of Medicine, 80 E Concord St, Boston, MA 02118, USA
Mol Pharmacol 70:779-85. 2006..By definition, obesity is too much fat, and we here review efforts to treat obesity and, by proxy, diabetes by modulating the metabolic state of adipocytes...- Cellular spelunking: exploring adipocyte caveolaePaul F Pilch
Department of Biochemistry, Boston University School of Medicine, Boston, MA 02118, USA
J Lipid Res 48:2103-11. 2007.... 
The mass action hypothesis: formation of Glut4 storage vesicles, a tissue-specific, regulated exocytic compartmentP F Pilch
Department of Biochemistry, Boston University School of Medicine, Boston, MA 02118, USA
Acta Physiol (Oxf) 192:89-101. 2008..I review herein the ontogeny of GSVs and their composition as it relates to a tissue-specific, hormone-sensitive exocytic compartment and propose a mechanism for their formation...
Fat caves: caveolae, lipid trafficking and lipid metabolism in adipocytesPaul F Pilch
Department of Biochemistry Boston University School of Medicine, 72 East Concord St, Boston, MA 02118, USA
Trends Endocrinol Metab 22:318-24. 2011..In this review, we examine the evidence supporting the role of caveolae in adipocyte lipid metabolism in the context of the protein and lipid composition of these structures...- Proteomic analysis of GLUT4 storage vesicles reveals LRP1 to be an important vesicle component and target of insulin signalingMark P Jedrychowski
Department of Biochemistry, Boston University Medical School, Boston, Massachusetts 02118, USA
J Biol Chem 285:104-14. 2010..Furthermore, adipose-specific LRP1 knock-out mice also exhibit decreased GLUT4 expression. These findings suggest LRP1 is an important component of GSVs, and its expression is needed for the formation of fully functional GSVs... - Cholesterol depletion in adipocytes causes caveolae collapse concomitant with proteosomal degradation of cavin-2 in a switch-like fashionMichael R Breen
Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts, United States of America
PLoS ONE 7:e34516. 2012..Taken together, these data demonstrate that cavin-2 functions as a cholesterol responsive component of caveolae that is required for cavin-1 localization to the plasma membrane, and caveolae structural integrity... - p115 Interacts with the GLUT4 vesicle protein, IRAP, and plays a critical role in insulin-stimulated GLUT4 translocationToshio Hosaka
Department of Biochemistry, Boston University School of Medicine, Boston, MA 02118, USA
Mol Biol Cell 16:2882-90. 2005..These data suggest that p115 has an important and specific role in insulin-stimulated Glut4 translocation, probably by way of tethering insulin-sensitive Glut4 vesicles at an as yet unknown intracellular site... - Caveolins/caveolae protect adipocytes from fatty acid-mediated lipotoxicityTova Meshulam
Department of Biochemistry, Boston University School of Medicine, Boston, MA, USA
J Lipid Res 52:1526-32. 2011.... - Deletion of Cavin/PTRF causes global loss of caveolae, dyslipidemia, and glucose intoleranceLibin Liu
Department of Biochemistry, Boston University School of Medicine, 715 Albany Street, Boston, MA 02118, USA
Cell Metab 8:310-7. 2008..Our results underscore the multiorgan role of caveolae in metabolic regulation and the obligate presence of Cavin for caveolae formation... - Dynamics of lipid droplet-associated proteins during hormonally stimulated lipolysis in engineered adipocytes: stabilization and lipid droplet binding of adipocyte differentiation-related protein/adipophilinDanielle N Gross
Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118, USA
Mol Endocrinol 20:459-66. 2006.... - A critical role of cavin (polymerase I and transcript release factor) in caveolae formation and organizationLibin Liu
Department of Biochemistry, Boston University Medical School, Boston, Massachusetts 02118, USA
J Biol Chem 283:4314-22. 2008..We propose that the presence of cavin on the inside surface of caveolae stabilizes these structures, probably through interaction with the cytoskeleton, and cavin therefore plays an important role in caveolae formation and organization... - Role of insulin-dependent cortical fodrin/spectrin remodeling in glucose transporter 4 translocation in rat adipocytesLibin Liu
Department of Biochemistry, Boston University Medical School, Boston, MA 02118, USA
Mol Biol Cell 17:4249-56. 2006..Together, our data suggest that insulin induces remodeling of the fodrin-actin network, which is required for the fusion of GLUT4 storage vesicles with the plasma membrane by permitting their access to the t-SNARE syntaxin 4... - Caveolins sequester FA on the cytoplasmic leaflet of the plasma membrane, augment triglyceride formation, and protect cells from lipotoxicityJeffrey R Simard
Department of Biophysics and Physiology, Boston University School of Medicine, Boston, MA, USA
J Lipid Res 51:914-22. 2010.... - Immunopurification and characterization of rat adipocyte caveolae suggest their dissociation from insulin signalingRicardo P Souto
Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118, USA
J Biol Chem 278:18321-9. 2003..The results are consistent with a role for caveolae in lipid flux in and of adipocytes... - Glut4 storage vesicles without Glut4: transcriptional regulation of insulin-dependent vesicular trafficDanielle N Gross
Department of Biochemistry, Boston University School of Medicine, MA 02118, USA
Mol Cell Biol 24:7151-62. 2004..The differentiated cells possess a large insulin-sensitive vesicular compartment with negligible Glut4, and Glut4 translocation can be reconstituted on expression of this transporter... - The sugar is sIRVed: sorting Glut4 and its fellow travelersKonstantin V Kandror
Department of Biochemistry, Boston University School of Medicine, 72 E Concord Street, Boston, MA 02118, USA
Traffic 12:665-71. 2011.... - The interaction of Akt with APPL1 is required for insulin-stimulated Glut4 translocationTsugumichi Saito
Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118, USA
J Biol Chem 282:32280-7. 2007..These data suggest that APPL1 plays an important role in insulin-stimulated Glut4 translocation in muscle and adipose tissues and that its N-terminal portion may be critical for APPL1 function... - Isolation of GLUT4 storage vesiclesKonstantin V Kandror
Boston University School of Medicine, Boston, Massachusetts, USA
Curr Protoc Cell Biol . 2006..The various protocols are applicable to cultured and primary adipocytes as well as skeletal muscle, the major insulin target cells expressing GLUT4... - Role of caveolin-1 and cholesterol in transmembrane fatty acid movementTova Meshulam
Department of Biochemistry, Boston University School of Medicine, 715 Albany Street, Boston, Massachusetts 02118, USA
Biochemistry 45:2882-93. 2006..We conclude that transport of FA across the plasma membrane is modulated by caveolin-1 and cholesterol and is not dependent on the putative FA transport proteins CD36 and FATP... - Phosphatidylinositol 4-kinase is a component of glucose transporter (GLUT 4)-containing vesiclesR L Del Vecchio
Department of Biochemistry, Boston University School of Medicine, Massachusetts 02118
J Biol Chem 266:13278-83. 1991..We postulate that while the GTV-PtdIns 4-kinase is not regulated by insulin, it may play a role in defining the fusogenic properties necessary to mediate membrane movement between the GTVs, plasma membranes, and microsomes... - Dynamics of protein-tyrosine phosphatases in rat adipocytesM R Calera
Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118, USA
J Biol Chem 275:6308-12. 2000.... - C2C12 myocytes lack an insulin-responsive vesicular compartment despite dexamethasone-induced GLUT4 expressionLori L Tortorella
Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118, USA
Am J Physiol Endocrinol Metab 283:E514-24. 2002..Therefore, these data support the hypothesis that GLUT4 expression by itself is insufficient to establish an insulin-sensitive vesicular compartment... - Insulin increases the association of Akt-2 with Glut4-containing vesiclesM R Calera
Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118, USA
J Biol Chem 273:7201-4. 1998..5-fold increase in Akt-2 in response to insulin relative to the amount of Glut4. These results are consistent with the possibility that Akt-2 may be involved in Glut4 vesicle translocation... - Expression of the glucose transporter isoform GLUT 4 is insufficient to confer insulin-regulatable hexose uptake to cultured muscle cellsN Kotliar
Department of Biochemistry, Boston University School of Medicine, Massachusetts 02118
Mol Endocrinol 6:337-45. 1992..These results suggest that acute insulin stimulation of glucose transport is not solely dependent on the presence of the insulin receptor and the GLUT 4 protein, and that the presence of some additional protein(s) must be required... - The Formin family protein, formin homolog overexpressed in spleen, interacts with the insulin-responsive aminopeptidase and profilin IIaHideaki Tojo
Discovery Research Laboratories II, Pharmaceutical Research Division, Takeda Chemical Industries Co, Ltd, Tsukuba, Ibaraki 300 4293, Japan
Mol Endocrinol 17:1216-29. 2003..These findings suggest that FHOS mediates an interaction between GLUT4/IRAP-containing vesicles and the cytoskeleton and may participate in exocytosis and/or retention of this membrane compartment... - Regulation of glycogen concentration and glycogen synthase activity in skeletal muscle of insulin-resistant ratsLise Coderre
The Montreal Diabetes Research Centre, Centre hospitalier de l Université de Montréal CHUM Hôtel Dieu and Department of Medicine, Universite de Montreal, 3850 St Urbain, Montreal, Que, Canada H2W 1T8
Arch Biochem Biophys 464:144-50. 2007..Furthermore, our results suggest that sucrose treatment may modulate dexamethasone action in skeletal muscle... - Rapid flip-flop of oleic acid across the plasma membrane of adipocytesFrits Kamp
Obesity Research Center, Boston Medical Center, Massachusetts 02118, USA
J Biol Chem 278:7988-95. 2003..Any postulated transport mechanism for facilitating translocation of fatty acid across the plasma membrane of adipocytes, including a protein transporter, would have to compete with the highly effective flip-flop mechanism...