FRANK OPPENHEIM

Summary

Affiliation: Boston University
Country: USA

Publications

  1. pmc Anti-candidal activity of genetically engineered histatin variants with multiple functional domains
    Frank G Oppenheim
    Department of Periodontology and Oral Biology, Boston University Henry M Goldman School of Dental Medicine, Boston, Massachusetts, USA
    PLoS ONE 7:e51479. 2012
  2. ncbi request reprint Salivary proteome and its genetic polymorphisms
    Frank G Oppenheim
    Department of Periodontology and Oral Biology, Boston University, Goldman School of Dental Medicine, Boston, Massachusetts 02118, USA
    Ann N Y Acad Sci 1098:22-50. 2007
  3. pmc Mass spectrometric identification of key proteolytic cleavage sites in statherin affecting mineral homeostasis and bacterial binding domains
    Eva J Helmerhorst
    Department of Periodontology and Oral Biology, Henry M Goldman School of Dental Medicine, Boston, Massachusetts 02118, USA
    J Proteome Res 9:5413-21. 2010
  4. pmc Large-scale phosphoproteome of human whole saliva using disulfide-thiol interchange covalent chromatography and mass spectrometry
    Erdjan Salih
    Department of Periodontology and Oral Biology, Henry M Goldman School of Dental Medicine, Boston University Medical Center, Boston, MA 02118, USA
    Anal Biochem 407:19-33. 2010
  5. pmc Identification of Lys-Pro-Gln as a novel cleavage site specificity of saliva-associated proteases
    Eva J Helmerhorst
    Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, Boston, MA 02118, USA
    J Biol Chem 283:19957-66. 2008
  6. pmc Discovery of a novel and rich source of gluten-degrading microbial enzymes in the oral cavity
    Eva J Helmerhorst
    Department of Periodontology and Oral Biology, Boston University Henry M Goldman School of Dental Medicine, Boston, Massachusetts, United States of America
    PLoS ONE 5:e13264. 2010
  7. pmc Roles of cellular respiration, CgCDR1, and CgCDR2 in Candida glabrata resistance to histatin 5
    Eva J Helmerhorst
    Department of Periodontology and Oral Biology, Goldman School of Dental Medicine, Boston University, 700 Albany St, CABR W 201, Boston, Massachusetts 02118, USA
    Antimicrob Agents Chemother 50:1100-3. 2006
  8. ncbi request reprint The concomitant expression and availability of conventional and alternative, cyanide-insensitive, respiratory pathways in Candida albicans
    Eva J Helmerhorst
    Department of Periodontology and Oral Biology, Goldman School of Dental Medicine, Boston University, 700 Albany Street W201, Boston, MA 02118, USA
    Mitochondrion 5:200-11. 2005
  9. ncbi request reprint Candida glabrata is unusual with respect to its resistance to cationic antifungal proteins
    Eva J Helmerhorst
    Department of Periodontology and Oral Biology, Goldman School of Dental Medicine, Boston University, 700 Albany Street, Boston, MA 02118, USA
    Yeast 22:705-14. 2005
  10. pmc Dialysis unmasks the fungicidal properties of glandular salivary secretions
    Eva J Helmerhorst
    Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, Boston, Massachusetts 02118, USA
    Infect Immun 72:2703-9. 2004

Research Grants

  1. SALIVARY PROTEINS IN DENTAL INTEGUMENTS
    FRANK OPPENHEIM; Fiscal Year: 2004
  2. MICROBICIDAL SALIVARY HISTIDINE RICH PROTEINS
    FRANK OPPENHEIM; Fiscal Year: 2000
  3. MICROBICIDAL SALIVARY HISTIDINE-RICH PROTEINS
    Frank G Oppenheim; Fiscal Year: 2010
  4. SALIVARY PROTEINS IN DENTAL INTEGUMENTS
    FRANK OPPENHEIM; Fiscal Year: 2009
  5. MICROBICIDAL SALIVARY HISTIDINE-RICH PROTEINS
    FRANK OPPENHEIM; Fiscal Year: 2006
  6. MICROBICIDAL SALIVARY HISTIDINE-RICH PROTEINS
    FRANK OPPENHEIM; Fiscal Year: 1993
  7. MICROBICIDAL SALIVARY HISTIDINE-RICH PROTEINS
    FRANK OPPENHEIM; Fiscal Year: 1990
  8. ANIONIC SALIVARY PROTEINS IN DENTAL INTEGUMENTS
    FRANK OPPENHEIM; Fiscal Year: 1990
  9. MICROBICIDAL SALIVARY HISTIDINE-RICH PROTEINS
    Frank G Oppenheim; Fiscal Year: 2011

Collaborators

Detail Information

Publications31

  1. pmc Anti-candidal activity of genetically engineered histatin variants with multiple functional domains
    Frank G Oppenheim
    Department of Periodontology and Oral Biology, Boston University Henry M Goldman School of Dental Medicine, Boston, Massachusetts, USA
    PLoS ONE 7:e51479. 2012
    ..The demonstrated increase in antifungal activity may provide an evolutionary explanation why such domain multiplication is a frequent event in human salivary proteins...
  2. ncbi request reprint Salivary proteome and its genetic polymorphisms
    Frank G Oppenheim
    Department of Periodontology and Oral Biology, Boston University, Goldman School of Dental Medicine, Boston, Massachusetts 02118, USA
    Ann N Y Acad Sci 1098:22-50. 2007
    ..Knowledge of the structure and function of salivary gland-derived proteins/peptides has a critical impact on the rapid and correct identification of biomarkers, whether they originate from exocrine or non-exocrine sources...
  3. pmc Mass spectrometric identification of key proteolytic cleavage sites in statherin affecting mineral homeostasis and bacterial binding domains
    Eva J Helmerhorst
    Department of Periodontology and Oral Biology, Henry M Goldman School of Dental Medicine, Boston, Massachusetts 02118, USA
    J Proteome Res 9:5413-21. 2010
    ..The full characterization of the statherin peptides generated facilitates the elucidation of their novel functional roles in the oral and gastro-intestinal environment...
  4. pmc Large-scale phosphoproteome of human whole saliva using disulfide-thiol interchange covalent chromatography and mass spectrometry
    Erdjan Salih
    Department of Periodontology and Oral Biology, Henry M Goldman School of Dental Medicine, Boston University Medical Center, Boston, MA 02118, USA
    Anal Biochem 407:19-33. 2010
    ..The origins and identity of WS phosphoproteome suggest significant implications for both basic science and the development of novel biomarkers/diagnostic tools for systemic and oral disease states...
  5. pmc Identification of Lys-Pro-Gln as a novel cleavage site specificity of saliva-associated proteases
    Eva J Helmerhorst
    Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, Boston, MA 02118, USA
    J Biol Chem 283:19957-66. 2008
    ..The P(1) site being occupied by a Gln residue is a nonarchetype with respect to known proteases and indicates the presence of novel glutamine-specific endoprotease(s) in oral fluid...
  6. pmc Discovery of a novel and rich source of gluten-degrading microbial enzymes in the oral cavity
    Eva J Helmerhorst
    Department of Periodontology and Oral Biology, Boston University Henry M Goldman School of Dental Medicine, Boston, Massachusetts, United States of America
    PLoS ONE 5:e13264. 2010
    ..Salivary microorganisms however exhibit glutamine endoprotease activity, discovered towards glutamine- and proline-rich salivary proteins. The aim was to explore if gliadins can serve as substrates for oral microbial enzymes...
  7. pmc Roles of cellular respiration, CgCDR1, and CgCDR2 in Candida glabrata resistance to histatin 5
    Eva J Helmerhorst
    Department of Periodontology and Oral Biology, Goldman School of Dental Medicine, Boston University, 700 Albany St, CABR W 201, Boston, Massachusetts 02118, USA
    Antimicrob Agents Chemother 50:1100-3. 2006
    ..Fluconazole resistance in this fungus is due in most cases to upregulation of CgCDR efflux pumps. We investigated whether the distinct resistance of C. glabrata to histatin 5 is related to similar mechanisms...
  8. ncbi request reprint The concomitant expression and availability of conventional and alternative, cyanide-insensitive, respiratory pathways in Candida albicans
    Eva J Helmerhorst
    Department of Periodontology and Oral Biology, Goldman School of Dental Medicine, Boston University, 700 Albany Street W201, Boston, MA 02118, USA
    Mitochondrion 5:200-11. 2005
    ..The availability of two electron pathways in C. albicans and the potential use of either pathway endows this pleomorphic fungus with another level at which it can rapidly adjust to altered environmental conditions...
  9. ncbi request reprint Candida glabrata is unusual with respect to its resistance to cationic antifungal proteins
    Eva J Helmerhorst
    Department of Periodontology and Oral Biology, Goldman School of Dental Medicine, Boston University, 700 Albany Street, Boston, MA 02118, USA
    Yeast 22:705-14. 2005
    ..Besides the well-described inherent resistance of C. glabrata to azole-derived antifungal agents, our studies indicate that this species is also able to withstand the otherwise detrimental activities of cationic antifungal proteins...
  10. pmc Dialysis unmasks the fungicidal properties of glandular salivary secretions
    Eva J Helmerhorst
    Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, Boston, Massachusetts 02118, USA
    Infect Immun 72:2703-9. 2004
    ..These results are consistent with the presence of lysozyme and histatins, respectively, which may represent the major candidacidal capacity of dialyzed parotid secretion...
  11. ncbi request reprint Characterization of the mitochondrial respiratory pathways in Candida albicans
    Eva J Helmerhorst
    Goldman School of Dental Medicine, Boston University, 100 East Newton Street, Boston, MA 02118, USA
    Biochim Biophys Acta 1556:73-80. 2002
    ....
  12. ncbi request reprint Human salivary gland-specific daily variations in histatin concentrations determined by a novel quantitation technique
    Heloisa Gusman
    Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, 100 East Newton Street, Boston, MA 02118, USA
    Arch Oral Biol 49:11-22. 2004
    ....
  13. ncbi request reprint Interaction of human salivary mucin MG2, its recombinant N-terminal region and a synthetic peptide with Actinobacillus actinomycetemcomitans
    Bing Liu
    Department of Biochemistry, Boston University School of Medicine, Boston, MA 02118, USA
    J Periodontal Res 37:416-24. 2002
    ..actinomycetemcomitans and that a 20 kDa fragment of MG2 occurs in whole saliva. This suggests that cleavage of MG2 in vivo may produce fragments with microbicidal properties and that this may represent a novel mechanism of host defense...
  14. pmc Kinetics of histatin proteolysis in whole saliva and the effect on bioactive domains with metal-binding, antifungal, and wound-healing properties
    Xiuli Sun
    Dept of Periodontology and Oral Biology, Goldman School of Dental Medicine, Boston University, Boston, MA 02118, USA
    FASEB J 23:2691-701. 2009
    ....
  15. ncbi request reprint Identification of protein components in in vivo human acquired enamel pellicle using LC-ESI-MS/MS
    Walter L Siqueira
    Department of Periodontology and Oral Biology, Goldman School of Dental Medicine, Boston University, Boston, Massachusetts 02118, USA
    J Proteome Res 6:2152-60. 2007
    ..A high intersubject consistency in pellicle composition was observed...
  16. ncbi request reprint Salivary micelles: identification of complexes containing MG2, sIgA, lactoferrin, amylase, glycosylated proline-rich protein and lysozyme
    Rodrigo V Soares
    Department of Biochemistry, Boston University School of Medicine, 80 East Concord Street K 312, Boston, MA 02118, USA
    Arch Oral Biol 49:337-43. 2004
    ....
  17. ncbi request reprint Structural characterisation of cysteines in a bacterial-binding motif of human salivary mucin MG2
    Rodrigo V Soares
    Department of Periodontology and Oral Biology, Boston University Medical Center, Boston, MA 02118, USA
    Arch Oral Biol 47:591-7. 2002
    ....
  18. ncbi request reprint Patterns of secretion of mucins and non-mucin glycoproteins in human submandibular/sublingual secretion
    Laura Becerra
    Department of Periodontology and Oral Biology, Goldman School of Dental Medicine, 700 Albany Street, Boston, MA 02118, USA
    Arch Oral Biol 48:147-54. 2003
    ..Finally, the present investigation has shown that the secretory patterns of mucins and non-mucin glycoproteins from submandibular/sublingual glands are complex and represent an important aspect of salivary gland physiology...
  19. ncbi request reprint Tandem mass spectrometry for structural characterization of proline-rich proteins: application to salivary PRP-3
    Nancy Leymarie
    Department of Biochemistry, Boston University School of Medicine, Massachusetts 02118, USA
    Anal Chem 74:4124-32. 2002
    ..A complementary approach using both decomposition methods provides more complete and interpretable sequence information and yielded >93% sequence coverage for the 11-kDa PRP-3 isolated from human saliva...
  20. pmc Small molecular weight proteins/peptides present in the in vivo formed human acquired enamel pellicle
    Walter L Siqueira
    Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, Boston University, 700 Albany Street, CABR, W201, Boston, MA 02118, United States
    Arch Oral Biol 54:437-44. 2009
    ..The aim of this study was to investigate the type and the nature of peptides present in the in vivo formed human acquired enamel pellicle...
  21. pmc Concentration and fate of histatins and acidic proline-rich proteins in the oral environment
    Melanie Campese
    Boston University, Goldman School of Dental Medicine, 700 Albany Street, CABR W 201, Boston, MA 02118, USA
    Arch Oral Biol 54:345-53. 2009
    ..6 min and 29.3+/-15.3 min, respectively (n=7). The data indicate that proteolysis in WS is an important factor in explaining the substantially lower concentrations of histatins and acidic PRPs in WS as compared to in glandular secretions...
  22. ncbi request reprint Expression of membrane-associated mucins MUC1 and MUC4 in major human salivary glands
    Bing Liu
    Department of Periodontology, Boston University Medical Center, Boston, Massachusetts 02118, USA
    J Histochem Cytochem 50:811-20. 2002
    ....
  23. ncbi request reprint Conformational changes in salivary proline-rich protein 1 upon adsorption to calcium phosphate crystals
    Satheesh Elangovan
    Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, Suite W201, 700 Albany Street, Boston, Massachusetts 02118 2392, USA
    Langmuir 23:11200-5. 2007
    ....
  24. ncbi request reprint Two-hybrid analysis of human salivary mucin MUC7 interactions
    Lucila S Bruno
    Department of Biochemistry, Boston University Medical Center, Boston, MA 02118, USA
    Biochim Biophys Acta 1746:65-72. 2005
    ....
  25. pmc Modulation of bone resorption by phosphorylation state of bone sialoprotein
    Paul Curtin
    Laboratory for the Study of Skeletal Disorders and Rehabilitation, Department of Orthopedic Research, Harvard Medical School and Children s Hospital, Boston, Massachusetts 02115, USA
    Biochemistry 48:6876-86. 2009
    ..The use of a live bone organ culture system augmented these results with further evidence that links the observed in vivo variable state of phosphorylation with bone remodeling...
  26. ncbi request reprint Identification of protein components in human acquired enamel pellicle and whole saliva using novel proteomics approaches
    Yuan Yao
    Department of Periodontology and Oral Biology, Boston University School of Dental Medicine, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Biol Chem 278:5300-8. 2003
    ..MS approaches allowed identification of not only well characterized salivary proteins but also novel proteins not previously identified in pellicle...
  27. ncbi request reprint Salivary histatins in human deep posterior lingual glands (of von Ebner)
    Marco Piludu
    Dipartimento di Citomorfologia, Universita degli Studi di Cagliari, Cagliari, Italy
    Arch Oral Biol 51:967-73. 2006
    ..In the present study we determined the expression and localisation of histatins in human posterior deep lingual glands (von Ebner's glands) by means of immunoelectron microscopy...
  28. ncbi request reprint Microsensor arrays for saliva diagnostics
    David R Walt
    Department of Chemistry, Tufts University, 62 Talbot Avenue, Medford, MA 02155, USA
    Ann N Y Acad Sci 1098:389-400. 2007
    ..These results are reported in the second part of this article...
  29. ncbi request reprint Electron microscopic immunogold localization of salivary mucins MG1 and MG2 in human submandibular and sublingual glands
    Marco Piludu
    Departimento di Citomorfologia, Universita degli Studi di Cagliari, Cagliari, Italy
    J Histochem Cytochem 51:69-79. 2003
    ..They also indicate differences in protein expression patterns among salivary serous cells...
  30. pmc Use of colorimetric test strips for monitoring the effect of hemodialysis on salivary nitrite and uric acid in patients with end-stage renal disease: a proof of principle
    Timothy M Blicharz
    Department of Chemistry, Tufts University, Medford, MA 02155, USA
    Clin Chem 54:1473-80. 2008
    ..We observed that salivary nitrite (NO(2)(-)) and uric acid (UA) concentrations consistently decreased as dialysis proceeded...
  31. ncbi request reprint Immunocytochemical localization of histatins in human salivary glands
    Mansur Ahmad
    Department of Diagnostic and Surgical Science, University of Minnesota, School of Dentistry, Minneapolis, Minnesota, USA
    J Histochem Cytochem 52:361-70. 2004
    ..They are also consistent with several previous studies demonstrating the variable distribution of different proteins within the granule content...

Research Grants40

  1. SALIVARY PROTEINS IN DENTAL INTEGUMENTS
    FRANK OPPENHEIM; Fiscal Year: 2004
    ....
  2. MICROBICIDAL SALIVARY HISTIDINE RICH PROTEINS
    FRANK OPPENHEIM; Fiscal Year: 2000
    ..Correlation of these data will permit a realistic assessment of the role of histatins under physiological conditions. ..
  3. MICROBICIDAL SALIVARY HISTIDINE-RICH PROTEINS
    Frank G Oppenheim; Fiscal Year: 2010
    ..The relevance of the proposed work is its ultimate goal of developing novel, effective and better tolerated antifungal drugs. ..
  4. SALIVARY PROTEINS IN DENTAL INTEGUMENTS
    FRANK OPPENHEIM; Fiscal Year: 2009
    ..abstract_text> ..
  5. MICROBICIDAL SALIVARY HISTIDINE-RICH PROTEINS
    FRANK OPPENHEIM; Fiscal Year: 2006
    ..albicans and 80 species of oral bacteria ranging from harmless commensal organisms to periodontal pathogens. ..
  6. MICROBICIDAL SALIVARY HISTIDINE-RICH PROTEINS
    FRANK OPPENHEIM; Fiscal Year: 1993
    ....
  7. MICROBICIDAL SALIVARY HISTIDINE-RICH PROTEINS
    FRANK OPPENHEIM; Fiscal Year: 1990
    ..5. Determine the concentration range of HRPs under stimulated and non-stimulated conditions in salivary secretions and in whole saliva of healthy subjects...
  8. ANIONIC SALIVARY PROTEINS IN DENTAL INTEGUMENTS
    FRANK OPPENHEIM; Fiscal Year: 1990
    ..The work proposed will provide the basis for understanding the protection or failure of protection of teeth in the oral cavity at a molecular level...
  9. MICROBICIDAL SALIVARY HISTIDINE-RICH PROTEINS
    Frank G Oppenheim; Fiscal Year: 2011
    ..The relevance of the proposed work is its ultimate goal of developing novel, effective and better tolerated antifungal drugs. ..