Genomes and Genes
Affiliation: Boston University
- Residual expression of reprogramming factors affects the transcriptional program and epigenetic signatures of induced pluripotent stem cellsCesar A Sommer
Section of Gastroenterology, Department of Medicine, Boston University School of Medicine, Boston, Massachusetts, United States of America
PLoS ONE 7:e51711. 2012..Our findings provide new insights into the reprogramming process and emphasize the importance of generating iPSCs free of any residual transgene expression...
- New directions in cellular therapy of cancer: a summary of the summit on cellular therapy for cancerDavid F Stroncek
Department of Transfusion Medicine, Clinical Center, NIH, Bethesda, USA
J Transl Med 10:48. 2012..In the future, combinations of adoptive transfer of T cells and specific vaccination against the cognate antigen can be envisaged to further enhance the effectiveness of these therapies...
- Shb deficient mice display an augmented TH2 response in peripheral CD4+ T cellsKarin Gustafsson
Department of Medical Cell Biology, Uppsala University, Sweden
BMC Immunol 12:3. 2011..A Shb knockout mouse was recently generated and the aim of the current study was to address the importance of Shb deficiency on T cell development and function...
- Concise review: The magic act of generating induced pluripotent stem cells: many rabbits in the hatGustavo Mostoslavsky
Section of Gastroenterology, Department of Medicine and Center for Regenerative Medicine CReM, Boston University School of Medicine, Boston, Massachusetts 02118, USA
Stem Cells 30:28-32. 2012....
- Excision of reprogramming transgenes improves the differentiation potential of iPS cells generated with a single excisable vectorCesar A Sommer
Section of Gastroenterology, Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118, USA
Stem Cells 28:64-74. 2010..In addition, our results underscore the consequences of long-term culture that will need to be taken into account for the clinical application of iPS cells...
- Mouse ES and iPS cells can form similar definitive endoderm despite differences in imprinted genesConstantina Christodoulou
Boston University Pulmonary Center, Boston University School of Medicine, Boston, Massachusetts 02118, USA
J Clin Invest 121:2313-25. 2011..Our results show that, despite some molecular differences, iPS cells can be efficiently differentiated into DE precursors, reinforcing their potential for development of cell-based therapies for diseased endoderm-derived tissues...
- Induced pluripotent stem cell generation using a single lentiviral stem cell cassetteCesar A Sommer
Department of Medicine, Boston University School of Medicine, Massachusetts 02118, USA
Stem Cells 27:543-9. 2009..The use of a single lentiviral vector for reprogramming represents a powerful laboratory tool and a significant step toward the application of iPS technology for clinical purposes...
- Differentiation stage determines potential of hematopoietic cells for reprogramming into induced pluripotent stem cellsSarah Eminli
Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, Boston, Massachusetts, USA
Nat Genet 41:968-76. 2009..Moreover, we identify hematopoietic progenitors as an attractive cell type for applications of iPS cell technology in research and therapy...
- Critical-size calvarial bone defects healing in a mouse model with silk scaffolds and SATB2-modified iPSCsJin Hai Ye
Division of Oral Biology, Tufts University School of Dental Medicine, One Kneeland Street, Boston, MA 02111, USA
Biomaterials 32:5065-76. 2011..In conclusion, the results demonstrate that SATB2 facilitates the differentiation of iPSCs towards osteoblast-lineage cells by repressing HoxA2 and augmenting the functions of the osteoblast determinants Runx2, BSP and OCN...
- Dual-promoter lentiviral system allows inducible expression of noxious proteins in macrophagesHui Pan
Department of Immunology and Infectious Diseases, Harvard School of Public Health, 667 Huntington Avenue, Boston, MA 02115, USA
J Immunol Methods 329:31-44. 2008..Our method will be especially useful to study immunity-related macrophage proteins in their physiological context during macrophage activation or infection...
- Complete correction of murine Artemis immunodeficiency by lentiviral vector-mediated gene transferGustavo Mostoslavsky
Department of Genetics, Harvard Medical School, and Division of Molecular Medicine, Children s Hospital, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 103:16406-11. 2006....
- Generation of transgene-free lung disease-specific human induced pluripotent stem cells using a single excisable lentiviral stem cell cassetteAba Somers
Boston University Pulmonary Center, Boston, Massachusetts 02118, USA
Stem Cells 28:1728-40. 2010..Moreover, we demonstrate that human iPSC generated with this approach have the ability to robustly differentiate into definitive endoderm in vitro, the developmental precursor tissue of lung epithelia...
- The aryl hydrocarbon receptor directs hematopoietic progenitor cell expansion and differentiationBrenden W Smith
Section of Hematology and Oncology, Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA
Blood 122:376-85. 2013....
- Application of induced pluripotent stem (iPS) cells in periodontal tissue regenerationXuejing Duan
Division of Oral Biology, Tufts University School of Dental Medicine, Boston, Massachusetts 02111, USA
J Cell Physiol 226:150-7. 2011..iPS cells combined with EMD provide a valuable tool for periodontal tissue engineering, by promoting the formation of new cementum, alveolar bone, and normal periodontal ligament...
- Exogenous control of mammalian gene expression via modulation of translational terminationGeorge J Murphy
Department of Genetics, Harvard Medical School, and Division of Molecular Medicine, Children s Hospital, Boston, Massachusetts 02115, USA
Nat Med 12:1093-9. 2006..This gene regulation strategy offers significant advantages over existing methods for controlling gene expression and should have both immediate experimental application and possible clinical application...
- The evolving field of induced pluripotency: recent progress and future challengesCesar A Sommer
Section of Gastroenterology, Department of Medicine and Center for Regenerative Medicine, Boston University School of Medicine, Boston, Massachusetts 02118, USA
J Cell Physiol 228:267-75. 2013....
- Tet1 and Tet2 regulate 5-hydroxymethylcytosine production and cell lineage specification in mouse embryonic stem cellsKian Peng Koh
Immune Disease Institute and Program in Cellular and Molecular Medicine, Children s Hospital Boston, Boston, MA 02115, USA
Cell Stem Cell 8:200-13. 2011..Thus, 5hmC is an epigenetic modification associated with the pluripotent state, and Tet1 functions to regulate the lineage differentiation potential of ESCs...
- PPARalpha deficiency in inflammatory cells suppresses tumor growthArja Kaipainen
Vascular Biology Program, Department of Surgery, Children s Hospital, Harvard Medical School, Boston, Massachusetts, United States of America
PLoS ONE 2:e260. 2007..These findings suggest that the absence of PPARalpha activity renders inflammatory infiltrates tumor suppressive and, thus, may provide a target for inhibiting tumor growth by modulating stromal processes, such as angiogenesis...
- Mitotic spindle destabilization and genomic instability in Shwachman-Diamond syndromeKaryn M Austin
Department of Pediatric Hematology, Children s Hospital Boston, Boston, Massachusetts, USA
J Clin Invest 118:1511-8. 2008..Conversely, SDS patient cells were resistant to taxol, a microtubule stabilizing agent. These findings suggest that spindle instability in SDS contributes to bone marrow failure and leukemogenesis...
- Induced pluripotent stem cells: a novel frontier in the study of human primary immunodeficienciesItai M Pessach
Division of Immunology, Children s Hospital Boston, Harvard Medical School, Boston, MA, USA
J Allergy Clin Immunol 127:1400-7.e4. 2011..The novel ability to epigenetically reprogram somatic cells into induced pluripotent stem cells (iPSCs) through the exogenous expression of transcription promises to revolutionize the study of human diseases...
- FSP27 and PLIN1 interaction promotes the formation of large lipid droplets in human adipocytesTan Hooi Min Grahn
Section of Endocrinology, Diabetes and Nutrition, Department of Medicine, Boston University School of Medicine, 650 Albany Street, Boston, MA 02118, USA
Biochem Biophys Res Commun 432:296-301. 2013..Our data suggest that FSP27 interacts with PLIN1 to regulate lipid droplet size in human adipocytes in a concerted manner...
- Efficiency of transduction of highly purified murine hematopoietic stem cells by lentiviral and oncoretroviral vectors under conditions of minimal in vitro manipulationGustavo Mostoslavsky
Department of Genetics, Harvard Medical School and Molecular Medicine, Children s Hospital, Boston, MA 02115, USA
Mol Ther 11:932-40. 2005..The use of a similar transduction/transplantation strategy in future clinical studies may offer distinct advantages over current protocols...
- Lack of immune response to differentiated cells derived from syngeneic induced pluripotent stem cellsPrajna Guha
NIH Center of Biomedical Research Excellence COBRE in Stem Cell Biology, Roger Williams Medical Center, Boston University School of Medicine, Providence, RI 02908, USA
Cell Stem Cell 12:407-12. 2013..Our data support the idea that differentiated cells generated from autologous iPSCs could be applied for cell replacement therapy without eliciting immune rejection...
- A mechanosensitive transcriptional mechanism that controls angiogenesisAkiko Mammoto
Vascular Biology Program, Department of Pathology and Surgery, Children s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
Nature 457:1103-8. 2009..This is, to our knowledge, the first known functional cross-antagonism between transcription factors that controls tissue morphogenesis, and that responds to both mechanical and chemical cues...
- Experimental approaches for the generation of induced pluripotent stem cellsCesar A Sommer
Section of Gastroenterology, Department of Medicine and Center for Regenerative Medicine CReM, Boston University School of Medicine, Boston, MA 02118, USA
Stem Cell Res Ther 1:26. 2010....