David Levin

..The interface of this signaling pathway with other pathways that contribute to the maintenance of cell wall integrity is also discussed...

..These results implicate Ypk1/2 in the regulation of phosphoinositides at the plasma membrane. The level at which this regulation takes place will be explored. ..

..Other known cell lysis mutants will also be examined for genetic interactions with PKC1 and BCK1...

..We have identified two redundant proteins whose loss of function results in cell lysis. We propose a multifaceted approach to establishing their role in the maintenance of cell wall integrity. ..

..Novel signaling components will be identified through synthetic lethal and suppressor screens using a conditional allele of the SIT4 gene, which encodes a Pl-4 kinase that has been implicated in PKC1 signaling. ..

..Proposed studies are likely to reveal suitable molecular targets for the development of antifungal agents that display selective toxicity against fungal cells. ..

..We have identified two redundant proteins whose loss of function results in cell lysis. We propose a multifaceted approach to establishing their role in the maintenance of cell wall integrity. ..

..These experiments will help to test the applicant's model of Rin1 function as an inhibitor of Ras signaling in the ER. ..