Darrell Kotton

Summary

Affiliation: Boston University
Country: USA

Publications

  1. pmc The prolonged life-span of alveolar macrophages
    Jaime Murphy
    The Pulmonary Center, R 304, Boston University School of Medicine, 715 Albany Street, Boston, MA 02118, USA
    Am J Respir Cell Mol Biol 38:380-5. 2008
  2. pmc Maintenance and repair of the lung endothelium does not involve contributions from marrow-derived endothelial precursor cells
    Sarah J Ohle
    The Pulmonary Center, Boston University School of Medicine, Boston, MA 02118, USA
    Am J Respir Cell Mol Biol 47:11-9. 2012
  3. doi request reprint Next-generation regeneration: the hope and hype of lung stem cell research
    Darrell N Kotton
    Pulmonary Center and Center for Regenerative Medicine CReM, Boston University and Boston Medical Center, 715 Albany St, R 304, Boston, MA 02118, USA
    Am J Respir Crit Care Med 185:1255-60. 2012
  4. ncbi request reprint Lung stem cells
    Darrell N Kotton
    Boston University Pulmonary Center, Boston University School of Medicine, 715 Albany Street, R 304, Boston, MA 02118, USA
    Cell Tissue Res 331:145-56. 2008
  5. pmc Failure of bone marrow to reconstitute lung epithelium
    Darrell N Kotton
    Department of Genetics, Harvard Medical School, Division of Molecular Medicine, Children s Hospital, Boston, MA 02115, USA
    Am J Respir Cell Mol Biol 33:328-34. 2005
  6. doi request reprint Excision of reprogramming transgenes improves the differentiation potential of iPS cells generated with a single excisable vector
    Cesar A Sommer
    Section of Gastroenterology, Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    Stem Cells 28:64-74. 2010
  7. pmc Dual-promoter lentiviral system allows inducible expression of noxious proteins in macrophages
    Hui Pan
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, 667 Huntington Avenue, Boston, MA 02115, USA
    J Immunol Methods 329:31-44. 2008
  8. pmc Amelioration of emphysema in mice through lentiviral transduction of long-lived pulmonary alveolar macrophages
    Andrew A Wilson
    Pulmonary Center and Department of Medicine, Boston University School of Medicine, 715 Albany Street, Boston, Massachusetts 02118, USA
    J Clin Invest 120:379-89. 2010
  9. pmc A novel stem-cell population in adult liver with potent hematopoietic-reconstitution activity
    Darrell N Kotton
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Blood 106:1574-80. 2005
  10. pmc Telomere dysfunction induces metabolic and mitochondrial compromise
    Ergun Sahin
    Belfer Institute for Applied Cancer Science, Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Nature 470:359-65. 2011

Collaborators

Detail Information

Publications24

  1. pmc The prolonged life-span of alveolar macrophages
    Jaime Murphy
    The Pulmonary Center, R 304, Boston University School of Medicine, 715 Albany Street, Boston, MA 02118, USA
    Am J Respir Cell Mol Biol 38:380-5. 2008
    ....
  2. pmc Maintenance and repair of the lung endothelium does not involve contributions from marrow-derived endothelial precursor cells
    Sarah J Ohle
    The Pulmonary Center, Boston University School of Medicine, Boston, MA 02118, USA
    Am J Respir Cell Mol Biol 47:11-9. 2012
    ..Our findings argue against any significant engraftment of bone marrow-derived cells in the pulmonary vascular endothelium...
  3. doi request reprint Next-generation regeneration: the hope and hype of lung stem cell research
    Darrell N Kotton
    Pulmonary Center and Center for Regenerative Medicine CReM, Boston University and Boston Medical Center, 715 Albany St, R 304, Boston, MA 02118, USA
    Am J Respir Crit Care Med 185:1255-60. 2012
    ..Exogenous derivation of lung epithelia from embryonic stem cells or induced pluripotent stem cells is also presented as an alternative method for engineering lung tissue de novo in culture...
  4. ncbi request reprint Lung stem cells
    Darrell N Kotton
    Boston University Pulmonary Center, Boston University School of Medicine, 715 Albany Street, R 304, Boston, MA 02118, USA
    Cell Tissue Res 331:145-56. 2008
    ..As a detailed understanding of stem cell biology, lung development, lineage commitment, and epithelial differentiation emerges, an ability to modulate lung injury and repair is likely to follow...
  5. pmc Failure of bone marrow to reconstitute lung epithelium
    Darrell N Kotton
    Department of Genetics, Harvard Medical School, Division of Molecular Medicine, Children s Hospital, Boston, MA 02115, USA
    Am J Respir Cell Mol Biol 33:328-34. 2005
    ....
  6. doi request reprint Excision of reprogramming transgenes improves the differentiation potential of iPS cells generated with a single excisable vector
    Cesar A Sommer
    Section of Gastroenterology, Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    Stem Cells 28:64-74. 2010
    ..In addition, our results underscore the consequences of long-term culture that will need to be taken into account for the clinical application of iPS cells...
  7. pmc Dual-promoter lentiviral system allows inducible expression of noxious proteins in macrophages
    Hui Pan
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, 667 Huntington Avenue, Boston, MA 02115, USA
    J Immunol Methods 329:31-44. 2008
    ..Our method will be especially useful to study immunity-related macrophage proteins in their physiological context during macrophage activation or infection...
  8. pmc Amelioration of emphysema in mice through lentiviral transduction of long-lived pulmonary alveolar macrophages
    Andrew A Wilson
    Pulmonary Center and Department of Medicine, Boston University School of Medicine, 715 Albany Street, Boston, Massachusetts 02118, USA
    J Clin Invest 120:379-89. 2010
    ..Our results challenge the dogma that AMs are short lived and suggest that these differentiated cells may be a possible target cell population for in vivo gene therapy applications, including the sustained correction of hAAT deficiency...
  9. pmc A novel stem-cell population in adult liver with potent hematopoietic-reconstitution activity
    Darrell N Kotton
    Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
    Blood 106:1574-80. 2005
    ..Overall, these studies have important implications for understanding extramedullary hematopoiesis, and may be relevant to current strategies aimed at inducing tolerance to transplanted organs...
  10. pmc Telomere dysfunction induces metabolic and mitochondrial compromise
    Ergun Sahin
    Belfer Institute for Applied Cancer Science, Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Nature 470:359-65. 2011
    ..We propose that this telomere-p53-PGC axis contributes to organ and metabolic failure and to diminishing organismal fitness in the setting of telomere dysfunction...
  11. pmc Epigenetic mechanisms modulate thyroid transcription factor 1-mediated transcription of the surfactant protein B gene
    YuXia Cao
    Pulmonary Center, Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    J Biol Chem 285:2152-64. 2010
    ..Our findings suggest that DNA methylation and chromatin modifications cooperate with Nkx2-1 to regulate Sftpb gene cell specific expression...
  12. ncbi request reprint miR-129 regulates cell proliferation by downregulating Cdk6 expression
    Junjie Wu
    Pulmonary Center, Department of Medicine, Boston University School of Medicine, Boston, MA, USA
    Cell Cycle 9:1809-18. 2010
    ..Together, our data indicate that miR-129 plays an important role in regulating cell proliferation by downregulation of Cdk6...
  13. pmc Sustained expression of alpha1-antitrypsin after transplantation of manipulated hematopoietic stem cells
    Andrew A Wilson
    The Pulmonary Center, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    Am J Respir Cell Mol Biol 39:133-41. 2008
    ..This lentiviral-based system may be useful for investigations requiring the systemic secretion of anti-proteases or cytokines relevant to the pathogenesis of a variety of lung diseases...
  14. ncbi request reprint Stem cell antigen-1 expression in the pulmonary vascular endothelium
    Darrell N Kotton
    The Pulmonary Center, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    Am J Physiol Lung Cell Mol Physiol 284:L990-6. 2003
    ..Overall, our data indicate that Sca-1 expression helps define the surface phenotype of endothelial cells throughout the pulmonary vasculature...
  15. ncbi request reprint Side population cells and Bcrp1 expression in lung
    Ross Summer
    The Pulmonary Center, Boston University School of Medicine, 80 E Concord St, Boston, MA 02118, USA
    Am J Physiol Lung Cell Mol Physiol 285:L97-104. 2003
    ..In BSMC, Bcrp1 was localized to an intracellular compartment, suggesting that the molecular site of Bcrp1 expression regulates SP phenotype...
  16. ncbi request reprint Lung stem cells: new paradigms
    Darrell N Kotton
    The Pulmonary Center, Boston University School of Medicine, Boston, MA 02118, USA
    Exp Hematol 32:340-3. 2004
    ..In this review, we explore the implications of these new studies for lung stem cell biology. We also summarize and discuss the ongoing controversies that these studies have generated...
  17. ncbi request reprint Embryonic lung side population cells are hematopoietic and vascular precursors
    Ross Summer
    The Pulmonary Center, Boston University School of Medicine, MA 02118, USA
    Am J Respir Cell Mol Biol 33:32-40. 2005
    ..Together, these findings illustrate that embryonic lung SP cells are heterogeneous, composed of hematopoeitic and nonhematopoeitic progenitors, and may play a key role in the formation of the lung vasculature...
  18. ncbi request reprint Efficiency of transduction of highly purified murine hematopoietic stem cells by lentiviral and oncoretroviral vectors under conditions of minimal in vitro manipulation
    Gustavo Mostoslavsky
    Department of Genetics, Harvard Medical School and Molecular Medicine, Children s Hospital, Boston, MA 02115, USA
    Mol Ther 11:932-40. 2005
    ..The use of a similar transduction/transplantation strategy in future clinical studies may offer distinct advantages over current protocols...
  19. doi request reprint Induced pluripotent stem cell generation using a single lentiviral stem cell cassette
    Cesar A Sommer
    Department of Medicine, Boston University School of Medicine, Massachusetts 02118, USA
    Stem Cells 27:543-9. 2009
    ..The use of a single lentiviral vector for reprogramming represents a powerful laboratory tool and a significant step toward the application of iPS technology for clinical purposes...
  20. doi request reprint Regeneration and orthotopic transplantation of a bioartificial lung
    Harald C Ott
    Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA
    Nat Med 16:927-33. 2010
    ..After transplantation, constructs were perfused by the recipient's circulation and ventilated by means of the recipient's airway and respiratory muscles, and they provided gas exchange in vivo for up to 6 h after extubation...
  21. ncbi request reprint Exogenous control of mammalian gene expression via modulation of translational termination
    George J Murphy
    Department of Genetics, Harvard Medical School, and Division of Molecular Medicine, Children s Hospital, Boston, Massachusetts 02115, USA
    Nat Med 12:1093-9. 2006
    ..This gene regulation strategy offers significant advantages over existing methods for controlling gene expression and should have both immediate experimental application and possible clinical application...
  22. doi request reprint Pulmonary alveolar proteinosis: a bench-to-bedside story of granulocyte-macrophage colony-stimulating factor dysfunction
    Sara R Greenhill
    Pulmonary Center, Boston University School of Medicine, Boston, MA 02118, USA
    Chest 136:571-7. 2009
    ....
  23. doi request reprint Another notch in stem cell biology: Drosophila intestinal stem cells and the specification of cell fates
    Andrew A Wilson
    Boston University School of Medicine, Boston, MA 02118, USA
    Bioessays 30:107-9. 2008
    ..These observations made in Drosophila are challenging and advancing our understanding of stem cell biology...
  24. ncbi request reprint Translational physiology: origin and phenotype of lung side population cells
    Ross Summer
    Am J Physiol Lung Cell Mol Physiol 287:L477-83. 2004
    ..In summary, these findings demonstrate that lung SP cells are derived from the BM and that CD45-positive and -negative subtypes can be distinguished by morphological differences and gene expression patterns...

Research Grants5

  1. Derivation of Lung Epithelium from Bone Marrow
    Darrell Kotton; Fiscal Year: 2007
    ....
  2. Cell-specific Delivery of RNAi to Pulmonary Alveolar Macrophages in vivo
    Darrell Kotton; Fiscal Year: 2007
    ..Finally, the system is applied for in vivo knockdown of NF-kB signaling in mouse alveolar macrophages and the treatment of tobacco smoke- induced lung inflammation. ..
  3. Derivation of Transplantable Lung Epithelial Progenitors from iPS Cells
    Darrell N Kotton; Fiscal Year: 2010
    ..This proposal develops novel therapies, based on infusions of lung progenitors derived from induced pluripotent stem (iPS) cells, designed to rescue and repair the injured lining of lung alveoli. ..