EVA JOSEPHINE HELMERHORST

Summary

Affiliation: Boston University
Country: USA

Publications

  1. ncbi request reprint Characterization of histatin 5 with respect to amphipathicity, hydrophobicity, and effects on cell and mitochondrial membrane integrity excludes a candidacidal mechanism of pore formation
    E J Helmerhorst
    Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, Boston, Massachusetts 02118, USA
    J Biol Chem 276:5643-9. 2001
  2. pmc Identification of Lys-Pro-Gln as a novel cleavage site specificity of saliva-associated proteases
    Eva J Helmerhorst
    Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, Boston, MA 02118, USA
    J Biol Chem 283:19957-66. 2008
  3. ncbi request reprint Saliva: a dynamic proteome
    E J Helmerhorst
    Boston University Goldman School of Dental Medicine, Department of Periodontology and Oral Biology, 700 Albany Street CABR W 201, Boston, MA 02118, USA
    J Dent Res 86:680-93. 2007
  4. ncbi request reprint Whole saliva proteolysis: wealth of information for diagnostic exploitation
    Eva J Helmerhorst
    Department of Periodontology and Oral Biology, Boston University, Goldman School of Dental Medicine, Boston, MA 02118, USA
    Ann N Y Acad Sci 1098:454-60. 2007
  5. pmc Discovery of a novel and rich source of gluten-degrading microbial enzymes in the oral cavity
    Eva J Helmerhorst
    Department of Periodontology and Oral Biology, Boston University Henry M Goldman School of Dental Medicine, Boston, Massachusetts, United States of America
    PLoS ONE 5:e13264. 2010
  6. ncbi request reprint Oral fluid proteolytic effects on histatin 5 structure and function
    E J Helmerhorst
    Department of Periodontology and Oral Biology, Boston University, Goldman School of Dental Medicine, 700 Albany Street, Boston, MA 02118, USA
    Arch Oral Biol 51:1061-70. 2006
  7. pmc Roles of cellular respiration, CgCDR1, and CgCDR2 in Candida glabrata resistance to histatin 5
    Eva J Helmerhorst
    Department of Periodontology and Oral Biology, Goldman School of Dental Medicine, Boston University, 700 Albany St, CABR W 201, Boston, Massachusetts 02118, USA
    Antimicrob Agents Chemother 50:1100-3. 2006
  8. ncbi request reprint The concomitant expression and availability of conventional and alternative, cyanide-insensitive, respiratory pathways in Candida albicans
    Eva J Helmerhorst
    Department of Periodontology and Oral Biology, Goldman School of Dental Medicine, Boston University, 700 Albany Street W201, Boston, MA 02118, USA
    Mitochondrion 5:200-11. 2005
  9. pmc The human salivary peptide histatin 5 exerts its antifungal activity through the formation of reactive oxygen species
    E J Helmerhorst
    Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, 100 East Newton Street, Boston, MA 02118 2392, USA
    Proc Natl Acad Sci U S A 98:14637-42. 2001
  10. pmc Dialysis unmasks the fungicidal properties of glandular salivary secretions
    Eva J Helmerhorst
    Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, Boston, Massachusetts 02118, USA
    Infect Immun 72:2703-9. 2004

Research Grants

  1. Gastro-intestinal microbes degrading dietary gluten
    EVA JOSEPHINE HELMERHORST; Fiscal Year: 2010
  2. Oral Yeast Carriage and Salivary Antifungal Activity
    EVA HELMERHORST; Fiscal Year: 2007

Collaborators

Detail Information

Publications19

  1. ncbi request reprint Characterization of histatin 5 with respect to amphipathicity, hydrophobicity, and effects on cell and mitochondrial membrane integrity excludes a candidacidal mechanism of pore formation
    E J Helmerhorst
    Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, Boston, Massachusetts 02118, USA
    J Biol Chem 276:5643-9. 2001
    ..These data indicate that the salivary protein histatin 5 exerts its antifungal function through a mechanism other than pore formation...
  2. pmc Identification of Lys-Pro-Gln as a novel cleavage site specificity of saliva-associated proteases
    Eva J Helmerhorst
    Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, Boston, MA 02118, USA
    J Biol Chem 283:19957-66. 2008
    ..The P(1) site being occupied by a Gln residue is a nonarchetype with respect to known proteases and indicates the presence of novel glutamine-specific endoprotease(s) in oral fluid...
  3. ncbi request reprint Saliva: a dynamic proteome
    E J Helmerhorst
    Boston University Goldman School of Dental Medicine, Department of Periodontology and Oral Biology, 700 Albany Street CABR W 201, Boston, MA 02118, USA
    J Dent Res 86:680-93. 2007
    ....
  4. ncbi request reprint Whole saliva proteolysis: wealth of information for diagnostic exploitation
    Eva J Helmerhorst
    Department of Periodontology and Oral Biology, Boston University, Goldman School of Dental Medicine, Boston, MA 02118, USA
    Ann N Y Acad Sci 1098:454-60. 2007
    ..We propose that the rate and mode of glandular salivary protein degradation in the oral cavity contains information that could be of unique value in the diagnosis of oral disease...
  5. pmc Discovery of a novel and rich source of gluten-degrading microbial enzymes in the oral cavity
    Eva J Helmerhorst
    Department of Periodontology and Oral Biology, Boston University Henry M Goldman School of Dental Medicine, Boston, Massachusetts, United States of America
    PLoS ONE 5:e13264. 2010
    ..Salivary microorganisms however exhibit glutamine endoprotease activity, discovered towards glutamine- and proline-rich salivary proteins. The aim was to explore if gliadins can serve as substrates for oral microbial enzymes...
  6. ncbi request reprint Oral fluid proteolytic effects on histatin 5 structure and function
    E J Helmerhorst
    Department of Periodontology and Oral Biology, Boston University, Goldman School of Dental Medicine, 700 Albany Street, Boston, MA 02118, USA
    Arch Oral Biol 51:1061-70. 2006
    ..The data also reveal that the early proteolysis phase of histatin 5 does not abolish the antifungal properties associated with this protein...
  7. pmc Roles of cellular respiration, CgCDR1, and CgCDR2 in Candida glabrata resistance to histatin 5
    Eva J Helmerhorst
    Department of Periodontology and Oral Biology, Goldman School of Dental Medicine, Boston University, 700 Albany St, CABR W 201, Boston, Massachusetts 02118, USA
    Antimicrob Agents Chemother 50:1100-3. 2006
    ..Fluconazole resistance in this fungus is due in most cases to upregulation of CgCDR efflux pumps. We investigated whether the distinct resistance of C. glabrata to histatin 5 is related to similar mechanisms...
  8. ncbi request reprint The concomitant expression and availability of conventional and alternative, cyanide-insensitive, respiratory pathways in Candida albicans
    Eva J Helmerhorst
    Department of Periodontology and Oral Biology, Goldman School of Dental Medicine, Boston University, 700 Albany Street W201, Boston, MA 02118, USA
    Mitochondrion 5:200-11. 2005
    ..The availability of two electron pathways in C. albicans and the potential use of either pathway endows this pleomorphic fungus with another level at which it can rapidly adjust to altered environmental conditions...
  9. pmc The human salivary peptide histatin 5 exerts its antifungal activity through the formation of reactive oxygen species
    E J Helmerhorst
    Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, 100 East Newton Street, Boston, MA 02118 2392, USA
    Proc Natl Acad Sci U S A 98:14637-42. 2001
    ..These data provide strong evidence for a comprehensive mechanistic model of histatin-5-provoked yeast cell death in which oxygen radical formation is the ultimate and essential step...
  10. pmc Dialysis unmasks the fungicidal properties of glandular salivary secretions
    Eva J Helmerhorst
    Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, Boston, Massachusetts 02118, USA
    Infect Immun 72:2703-9. 2004
    ..These results are consistent with the presence of lysozyme and histatins, respectively, which may represent the major candidacidal capacity of dialyzed parotid secretion...
  11. ncbi request reprint Candida glabrata is unusual with respect to its resistance to cationic antifungal proteins
    Eva J Helmerhorst
    Department of Periodontology and Oral Biology, Goldman School of Dental Medicine, Boston University, 700 Albany Street, Boston, MA 02118, USA
    Yeast 22:705-14. 2005
    ..Besides the well-described inherent resistance of C. glabrata to azole-derived antifungal agents, our studies indicate that this species is also able to withstand the otherwise detrimental activities of cationic antifungal proteins...
  12. ncbi request reprint Characterization of the mitochondrial respiratory pathways in Candida albicans
    Eva J Helmerhorst
    Goldman School of Dental Medicine, Boston University, 100 East Newton Street, Boston, MA 02118, USA
    Biochim Biophys Acta 1556:73-80. 2002
    ....
  13. pmc Large-scale phosphoproteome of human whole saliva using disulfide-thiol interchange covalent chromatography and mass spectrometry
    Erdjan Salih
    Department of Periodontology and Oral Biology, Henry M Goldman School of Dental Medicine, Boston University Medical Center, Boston, MA 02118, USA
    Anal Biochem 407:19-33. 2010
    ..The origins and identity of WS phosphoproteome suggest significant implications for both basic science and the development of novel biomarkers/diagnostic tools for systemic and oral disease states...
  14. pmc Mass spectrometric identification of key proteolytic cleavage sites in statherin affecting mineral homeostasis and bacterial binding domains
    Eva J Helmerhorst
    Department of Periodontology and Oral Biology, Henry M Goldman School of Dental Medicine, Boston, Massachusetts 02118, USA
    J Proteome Res 9:5413-21. 2010
    ..The full characterization of the statherin peptides generated facilitates the elucidation of their novel functional roles in the oral and gastro-intestinal environment...
  15. ncbi request reprint Salivary proteome and its genetic polymorphisms
    Frank G Oppenheim
    Department of Periodontology and Oral Biology, Boston University, Goldman School of Dental Medicine, Boston, Massachusetts 02118, USA
    Ann N Y Acad Sci 1098:22-50. 2007
    ..Knowledge of the structure and function of salivary gland-derived proteins/peptides has a critical impact on the rapid and correct identification of biomarkers, whether they originate from exocrine or non-exocrine sources...
  16. ncbi request reprint Human salivary gland-specific daily variations in histatin concentrations determined by a novel quantitation technique
    Heloisa Gusman
    Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, 100 East Newton Street, Boston, MA 02118, USA
    Arch Oral Biol 49:11-22. 2004
    ....
  17. pmc Kinetics of histatin proteolysis in whole saliva and the effect on bioactive domains with metal-binding, antifungal, and wound-healing properties
    Xiuli Sun
    Dept of Periodontology and Oral Biology, Goldman School of Dental Medicine, Boston University, Boston, MA 02118, USA
    FASEB J 23:2691-701. 2009
    ....
  18. ncbi request reprint Identification of protein components in in vivo human acquired enamel pellicle using LC-ESI-MS/MS
    Walter L Siqueira
    Department of Periodontology and Oral Biology, Goldman School of Dental Medicine, Boston University, Boston, Massachusetts 02118, USA
    J Proteome Res 6:2152-60. 2007
    ..A high intersubject consistency in pellicle composition was observed...
  19. pmc Concentration and fate of histatins and acidic proline-rich proteins in the oral environment
    Melanie Campese
    Boston University, Goldman School of Dental Medicine, 700 Albany Street, CABR W 201, Boston, MA 02118, USA
    Arch Oral Biol 54:345-53. 2009
    ..6 min and 29.3+/-15.3 min, respectively (n=7). The data indicate that proteolysis in WS is an important factor in explaining the substantially lower concentrations of histatins and acidic PRPs in WS as compared to in glandular secretions...

Research Grants4

  1. Gastro-intestinal microbes degrading dietary gluten
    EVA JOSEPHINE HELMERHORST; Fiscal Year: 2010
    ..The current application seeks to identify gluten-neutralizing enzymes expressed by resident microbes from the human gastrointestinal tract. ..
  2. Oral Yeast Carriage and Salivary Antifungal Activity
    EVA HELMERHORST; Fiscal Year: 2007
    ..The experiments outlined here will provide the basis for a better understanding of antifungal mechanisms exerted by saliva in the oral cavity. ..