Research Topics
Genomes and Genes | Roberto DominguezSummaryAffiliation: Boston Biomedical Research Institute Country: USA Publications
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Publications
Actin-binding proteins--a unifying hypothesisRoberto Dominguez
Boston Biomedical Research Institute, 64 Grove Street, Watertown, MA 02472, USA
Trends Biochem Sci 29:572-8. 2004..As the pocket remains accessible in F-actin, it is proposed that this pocket represents a primary target for F-actin-binding proteins, such as calponin-homology-related proteins and myosin...
Structure of the light chain-binding domain of myosin VMohammed Terrak
Boston Biomedical Research Institute, 64 Grove Street, Watertown, MA 02472, USA
Proc Natl Acad Sci U S A 102:12718-23. 2005..The model points to a prominent role of the LCBD in the function, regulation, and molecular interactions of myosin V...- Two distinct myosin light chain structures are induced by specific variations within the bound IQ motifs-functional implicationsMohammed Terrak
Boston Biomedical Research Institute, 64 Grove Street, Watertown, MA 02472, USA
EMBO J 22:362-71. 2003..The resulting 'free' N-lobes of myosin light chains in the extended conformation could mediate the formation of ternary complexes during protein localization and/or partner recruitment... - Understanding the role of the G-actin-binding domain of Ena/VASP in actin assemblyDavid Chereau
Boston Biomedical Research Institute, 64 Grove Street, Watertown, MA 02472, USA
J Struct Biol 155:195-201. 2006..Conserved domain architecture suggests that WASP may work similarly... - Actin-bound structures of Wiskott-Aldrich syndrome protein (WASP)-homology domain 2 and the implications for filament assemblyDavid Chereau
Boston Biomedical Research Institute, Watertown, MA 02472, USA
Proc Natl Acad Sci U S A 102:16644-9. 2005..The structures also suggest a mechanism for how profilin-binding Pro-rich sequences positioned N-terminal to WH2 could feed actin monomers directly to WH2, thereby playing a role in filament elongation... - Crystal structure of the actin-binding domain of alpha-actinin 1: evaluating two competing actin-binding modelsEmma Borrego-Diaz
Boston Biomedical Research Institute, 64 Grove Street, Watertown, MA 02472, USA
J Struct Biol 155:230-8. 2006.... - Leiomodin is an actin filament nucleator in muscle cellsDavid Chereau
Boston Biomedical Research Institute, Watertown, MA 02472, USA
Science 320:239-43. 2008..Knocking down leiomodin severely compromised sarcomere assembly in cultured muscle cells, which suggests a role for leiomodin in the nucleation of tropomyosin-decorated filaments in muscles... - Crystal structure of monomeric actin in the ATP state. Structural basis of nucleotide-dependent actin dynamicsPhilip Graceffa
Boston Biomedical Research Institute, Watertown, Massachusetts 02472, USA
J Biol Chem 278:34172-80. 2003..An analysis of the existing structures of members of the actin superfamily suggests that the cleft is open in the nucleotide-free state... - Structural basis of protein phosphatase 1 regulationMohammed Terrak
Boston Biomedical Research Institute, 64 Grove Street, Watertown, Massachusetts 02472, USA
Nature 429:780-4. 2004..The structure has general implications for the control of PP1 activity by other regulatory subunits... - Crystal structures of the vitamin D-binding protein and its complex with actin: structural basis of the actin-scavenger systemLudovic R Otterbein
Boston Biomedical Research Institute, 64 Grove Street, Watertown, MA 02472, USA
Proc Natl Acad Sci U S A 99:8003-8. 2002..Formation of the strong actin-DBP complex proceeds with limited conformational changes to both proteins, demonstrating how DBP has evolved to become an effective actin-scavenger protein... - Crystallization, X-ray characterization and selenomethionine phasing of Mlc1p bound to IQ motifs from myosin VMohammed Terrak
Boston Biomedical Research Institute, 64 Grove Street, Watertown, Massachusetts 02472, USA
Acta Crystallogr D Biol Crystallogr 58:1882-5. 2002..The other two structures (Mlc1p-IQ2 and Mlc1p-IQ3) were determined by molecular replacement using the partially refined structure of Mlc1p-IQ2,3 as a search model... - Crystal structures of S100A6 in the Ca(2+)-free and Ca(2+)-bound states: the calcium sensor mechanism of S100 proteins revealed at atomic resolutionLudovic R Otterbein
Boston Biomedical Research Institute, 64 Grove Street, Watertown, MA 02472, USA
Structure 10:557-67. 2002..The structures have important implications for our understanding of target binding and cooperativity of Ca(2+) binding in the S100 family... - Cortactin binding to F-actin revealed by electron microscopy and 3D reconstructionKiran Pant
Department of Physiology and Biophysics, Boston University School of Medicine, 715 Albany Street, Boston, MA 02118, USA
J Mol Biol 359:840-7. 2006..In addition, cortactin may act as a structural adapter protein, stabilizing nascent filament branches while mediating the simultaneous recruitment of Arp2/3 and WASP... - X-ray scattering study of activated Arp2/3 complex with bound actin-WCAMalgorzata Boczkowska
Department of Physiology, University of Pennsylvania School of Medicine, 3700 Hamilton Walk, A507 Richards Building, Philadelphia, PA 19104 6085, USA
Structure 16:695-704. 2008..The results support a model of activation that is consistent with most of the biochemical observations... - Crystal structure of the actin-binding domain of alpha-actinin-4 Lys255Glu mutant implicated in focal segmental glomerulosclerosisSung Haeng Lee
University of Pennsylvania School of Medicine, Department of Physiology, 3700 Hamilton Walk, Philadelphia, PA 19104 6085, USA
J Mol Biol 376:317-24. 2008..The absence of a structural change suggests that the compact ABD conformation observed in the majority of the structures is highly stable and is preserved in solution, even in FSGS mutant ABDs... - Structural basis for the recruitment of profilin-actin complexes during filament elongation by Ena/VASPFrancois Ferron
Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
EMBO J 26:4597-606. 2007..The actin monomer bound at the GAB domain is proposed to be in position to join the barbed end of the growing filament concurrently with the release of profilin... - The beta-thymosin/WH2 fold: multifunctionality and structureRoberto Dominguez
Department of Physiology, University of Pennsylvania School of Medicine, 3700 Hamilton Walk, Philadelphia, PA 19104, USA
Ann N Y Acad Sci 1112:86-94. 2007.... - Structural basis for the actin-binding function of missing-in-metastasisSung Haeng Lee
Department of Physiology, University of Pennsylvania School of Medicine, 3700 Hamilton Walk, Philadelphia, PA 19104, USA
Structure 15:145-55. 2007..The WH2 of MIM is longer than the prototypical WH2, interacting with all four subdomains of actin. We characterize a similar WH2 at the C terminus of IRSp53 and propose that in these two proteins WH2 performs a scaffolding function... - Solution properties of TMR-actin: when biochemical and crystal data agreeRoberto Dominguez
Biophys J 85:2073-4. 2003 - Toxofilin from Toxoplasma gondii forms a ternary complex with an antiparallel actin dimerSung Haeng Lee
Department of Physiology, University of Pennsylvania School of Medicine, 3700 Hamilton Walk, Philadelphia, PA 19104 6085, USA
Proc Natl Acad Sci U S A 104:16122-7. 2007..This effect is linked to the formation of the antiparallel actin dimer because a construct lacking helices 1 and 2 binds only one actin and inhibits nucleotide exchange less potently... - Multiple luteinizing hormone receptor (LHR) protein variants, interspecies reactivity of anti-LHR mAb clone 3B5, subcellular localization of LHR in human placenta, pelvic floor and brain, and possible role for LHR in the development of abnormal pregnancy,Antonin Bukovsky
Laboratory for Development, Differentiation and Cancer, Department of Obstetrics and Gynecology, The University of Tennessee Graduate School of Medicine, 1924 Alcoa Highway, Knoxville, TN 37920, USA
Reprod Biol Endocrinol 1:46. 2003.... - X-ray scattering study of actin polymerization nuclei assembled by tandem W domainsGrzegorz Rebowski
Department of Physiology, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
Proc Natl Acad Sci U S A 105:10785-90. 2008..The successful stabilization of filament-like multiactin assemblies opens the way to the crystallographic investigation of intersubunit contacts in the actin filament... - Modulation of actin structure and function by phosphorylation of Tyr-53 and profilin bindingKyuwon Baek
Department of Physiology, 3700 Hamilton Walk, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 6085, USA
Proc Natl Acad Sci U S A 105:11748-53. 2008..As a result, the nucleotide cleft becomes moderately more open in the profilin-actin complex, probably explaining the stimulation of nucleotide exchange on actin by profilin...