Barbara E Corkey

Summary

Affiliation: Boston University
Country: USA

Publications

  1. ncbi request reprint Acyl coenzyme a synthetase regulation: putative role in long-chain acyl coenzyme a partitioning
    Yan Lin Wang
    Department of Medicine, Obesity Research Center, Boston University School of Medicine, Boston, MA 02118, USA
    Obes Res 12:1781-8. 2004
  2. pmc Mitochondrial networking protects beta-cells from nutrient-induced apoptosis
    Anthony J A Molina
    Department of Molecular Medicine, Obesity Research Center, Boston University School of Medicine, Boston, Massachusetts, USA
    Diabetes 58:2303-15. 2009
  3. pmc Metabolic master regulators: sharing information among multiple systems
    Barbara E Corkey
    Obesity Research Center, Evans Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA
    Trends Endocrinol Metab 23:594-601. 2012
  4. pmc Banting lecture 2011: hyperinsulinemia: cause or consequence?
    Barbara E Corkey
    Obesity Research Center, Evans Department of Medicine, Boston University School of Medicine, Boston, Massachusetts, USA
    Diabetes 61:4-13. 2012
  5. pmc Reactive oxygen species facilitate translocation of hormone sensitive lipase to the lipid droplet during lipolysis in human differentiated adipocytes
    Sarah A Krawczyk
    Department of Medicine, Boston University School of Medicine, Boston, Massachusetts, United States of America
    PLoS ONE 7:e34904. 2012
  6. doi request reprint Respiration in adipocytes is inhibited by reactive oxygen species
    Tong Wang
    Obesity Research Center, Department of Medicine, Boston University School of Medicine, Boston, Massachusetts, USA
    Obesity (Silver Spring) 18:1493-502. 2010
  7. ncbi request reprint Regulation of acetyl CoA carboxylase and carnitine palmitoyl transferase-1 in rat adipocytes
    Yan Zang
    Obesity Research Center, Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA
    Obes Res 13:1530-9. 2005
  8. pmc Reactive oxygen species stimulate insulin secretion in rat pancreatic islets: studies using mono-oleoyl-glycerol
    Marylana Saadeh
    Department of Medicine, Obesity Research Center, School of Medicine, Boston University, Boston, Massachusetts, United States of America
    PLoS ONE 7:e30200. 2012
  9. ncbi request reprint Octanoate inhibits triglyceride synthesis in 3T3-L1 and human adipocytes
    Wen Guo
    Obesity Research Center, Department of Biophysics, Boston University School of Medicine, Boston, MA 02118, USA
    J Nutr 133:2512-8. 2003
  10. ncbi request reprint Glucose-dependent insulin modulation of oscillatory lipolysis in perifused rat adipocytes
    Lisa Getty-Kaushik
    Obesity Research Center, Boston Medical Center, MA 02118, USA
    Obes Res 13:2058-65. 2005

Research Grants

  1. METABOLIC REGULATION OF INSULIN SECRETION
    BARBARA CORKEY; Fiscal Year: 2005
  2. METABOLIC SIGNAL TRANSDUCTION IN ADIPOCYTES
    BARBARA CORKEY; Fiscal Year: 2007
  3. METABOLIC REGULATION OF INSULIN SECRETION
    BARBARA CORKEY; Fiscal Year: 2007
  4. METABOLIC SIGNAL TRANSDUCTION IN ADIPOCYTES
    Barbara E Corkey; Fiscal Year: 2010
  5. Lipid signal transduction /oscillatory insulin secretion
    BARBARA CORKEY; Fiscal Year: 2004
  6. METABOLIC REGULATION OF INSULIN SECRETION
    BARBARA CORKEY; Fiscal Year: 2003
  7. METABOLIC SIGNAL TRANSDUCTION IN ADIPOCYTES
    BARBARA CORKEY; Fiscal Year: 2005
  8. METABOLIC REGULATION OF INSULIN SECRETION
    Orian S Shirihai; Fiscal Year: 2010

Collaborators

Detail Information

Publications40

  1. ncbi request reprint Acyl coenzyme a synthetase regulation: putative role in long-chain acyl coenzyme a partitioning
    Yan Lin Wang
    Department of Medicine, Obesity Research Center, Boston University School of Medicine, Boston, MA 02118, USA
    Obes Res 12:1781-8. 2004
    ..ACSL-1 is highly expressed in adipose tissue with broad substrate specificity. We tested the hypothesis that ACSL localization, and resulting local generation of LC-CoA, regulates FFA partitioning...
  2. pmc Mitochondrial networking protects beta-cells from nutrient-induced apoptosis
    Anthony J A Molina
    Department of Molecular Medicine, Obesity Research Center, Boston University School of Medicine, Boston, Massachusetts, USA
    Diabetes 58:2303-15. 2009
    ..In this article, we investigate beta-cell mitochondrial fusion and fission in detail and report alterations in response to various combinations of nutrients...
  3. pmc Metabolic master regulators: sharing information among multiple systems
    Barbara E Corkey
    Obesity Research Center, Evans Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA
    Trends Endocrinol Metab 23:594-601. 2012
    ..Validation of this model would support simultaneous regulation of all collaborating metabolic organs through changes in the circulation, regardless of whether change was initiated exogenously or by a single organ...
  4. pmc Banting lecture 2011: hyperinsulinemia: cause or consequence?
    Barbara E Corkey
    Obesity Research Center, Evans Department of Medicine, Boston University School of Medicine, Boston, Massachusetts, USA
    Diabetes 61:4-13. 2012
    ..She presented the Banting Lecture, "Hyperinsulinemia: Cause or Consequence?" on Sunday, 26 June 2011...
  5. pmc Reactive oxygen species facilitate translocation of hormone sensitive lipase to the lipid droplet during lipolysis in human differentiated adipocytes
    Sarah A Krawczyk
    Department of Medicine, Boston University School of Medicine, Boston, Massachusetts, United States of America
    PLoS ONE 7:e34904. 2012
    ..These findings are significant because they document that ROS contributes to the physiological regulation of lipolysis via an effect on translocation. Such regulation could be useful in developing new obesity therapies...
  6. doi request reprint Respiration in adipocytes is inhibited by reactive oxygen species
    Tong Wang
    Obesity Research Center, Department of Medicine, Boston University School of Medicine, Boston, Massachusetts, USA
    Obesity (Silver Spring) 18:1493-502. 2010
    ..Stimulating fuel oxidation in adipocytes by decreasing ROS may provide a novel means to shift the balance from fuel storage to fuel burning...
  7. ncbi request reprint Regulation of acetyl CoA carboxylase and carnitine palmitoyl transferase-1 in rat adipocytes
    Yan Zang
    Obesity Research Center, Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA
    Obes Res 13:1530-9. 2005
    ..To test the hypothesis that both enzymes play critical roles in regulation of FFA partitioning in adipocytes, we compared enzyme mRNA expression and specific activity from fed, fasted, and diabetic rats...
  8. pmc Reactive oxygen species stimulate insulin secretion in rat pancreatic islets: studies using mono-oleoyl-glycerol
    Marylana Saadeh
    Department of Medicine, Obesity Research Center, School of Medicine, Boston University, Boston, Massachusetts, United States of America
    PLoS ONE 7:e30200. 2012
    ..These data suggest that basal insulin secretion is stimulated by increased ROS production, due to an increase in the mitochondrial redox state independent of the established components of GSIS...
  9. ncbi request reprint Octanoate inhibits triglyceride synthesis in 3T3-L1 and human adipocytes
    Wen Guo
    Obesity Research Center, Department of Biophysics, Boston University School of Medicine, Boston, MA 02118, USA
    J Nutr 133:2512-8. 2003
    ..Together, these results suggest that octanoate affected adipocyte function by reducing TG synthesis but not by enhancing oxidation...
  10. ncbi request reprint Glucose-dependent insulin modulation of oscillatory lipolysis in perifused rat adipocytes
    Lisa Getty-Kaushik
    Obesity Research Center, Boston Medical Center, MA 02118, USA
    Obes Res 13:2058-65. 2005
    ..This study examined the effect of insulin on this hypothesis...
  11. pmc Ca2+, NAD(P)H and membrane potential changes in pancreatic beta-cells by methyl succinate: comparison with glucose
    Emma Heart
    Obesity Research Center, Evans Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA
    Biochem J 403:197-205. 2007
    ..The data are consistent with the common intermediates being pyruvate, cytosolic NADPH or both, and suggest that cytosolic NADPH production could account for the more rapid onset of MeS-induced secretion compared with glucose stimulation...
  12. ncbi request reprint Aging results in paradoxical susceptibility of fat cell progenitors to lipotoxicity
    Wen Guo
    Evans Department of Medicine, Obesity Research Center, Boston University Medical Center, 88 E Newton St, Boston, MA 02118, USA
    Am J Physiol Endocrinol Metab 292:E1041-51. 2007
    ..Thus preadipocyte fatty acid handling changes with aging, with increased susceptibly to lipotoxicity and impaired fatty acid-induced adipogenesis and beta-oxidation...
  13. ncbi request reprint Regulation of lipolytic activity by long-chain acyl-coenzyme A in islets and adipocytes
    Liping Hu
    Obesity Research Center, Boston University School of Medicine, 650 Albany Street, Boston, MA 02118, USA
    Am J Physiol Endocrinol Metab 289:E1085-92. 2005
    ..Together, these opposite actions of LC-CoA lower circulating fat by inhibiting its release from adipocytes and promoting fat storage via insulin action...
  14. ncbi request reprint Oleate-induced formation of fat cells with impaired insulin sensitivity
    Weisheng Xie
    Obesity Research Center, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    Lipids 41:267-71. 2006
    ....
  15. ncbi request reprint beta-Cell mitochondria exhibit membrane potential heterogeneity that can be altered by stimulatory or toxic fuel levels
    Jakob D Wikstrom
    Tufts University, Department of Pharmacology and Experimental Therapeutics, 136 Harrison Ave, Boston, MA 02111, USA
    Diabetes 56:2569-78. 2007
    ..This study offers insight into the different metabolic states of beta-cell mitochondria...
  16. pmc Fission and selective fusion govern mitochondrial segregation and elimination by autophagy
    Gilad Twig
    Department of Pharmacology and Experimental Therapeutics, Tufts University School of Medicine, Boston, MA 02111, USA
    EMBO J 27:433-46. 2008
    ..Together, these findings suggest that fission followed by selective fusion segregates dysfunctional mitochondria and permits their removal by autophagy...
  17. ncbi request reprint Medium-chain Fatty acids attenuate agonist-stimulated lipolysis, mimicking the effects of starvation
    Tianguang Lei
    Obesity Research Center, Boston University School of Medicine, Boston, Massachusetts USA
    Obes Res 12:599-611. 2004
    ..To test the hypothesis that incorporation of medium-chain fatty acids (FAs) into adipocyte triglycerides alters intracellular lipolysis...
  18. pmc Glucose-dependent insulinotropic polypeptide enhances adipocyte development and glucose uptake in part through Akt activation
    Diane H Song
    Department of Medicine, Boston University School of Medicine and Boston Medical Center, Boston, Massachusetts, USA
    Gastroenterology 133:1796-805. 2007
    ..The purpose of these studies was to characterize the effects of GIP and its receptor (GIPR) in adipocyte development and signaling...
  19. ncbi request reprint Free fatty acid regulation of glucose-dependent intrinsic oscillatory lipolysis in perifused isolated rat adipocytes
    Lisa Getty-Kaushik
    Obesity Research Center, Department of Molecular Medicine, Boston Medical Center, EBRC 840, 650 Albany St, Boston, MA 02118, USA
    Diabetes 54:629-37. 2005
    ..The results contribute to the understanding of physiological and biochemical regulators of lipolysis, such as glucose and FFAs. Lipolytic oscillations may be beneficial in the delivery of FFAs to liver, pancreas, and other tissues...
  20. ncbi request reprint New scanning electron microscopic method for determination of adipocyte size in humans and mice
    Wen Guo
    Department of Medicine, Boston University School of Medicine, 650 Albany Streeet, Room 805, Boston, MA 02118, USA
    Obesity (Silver Spring) 15:1657-65. 2007
    ..To evaluate a new scanning electronic microscopic (EM) method for assessing fat cell sizes and compare fat cell size distribution in human adipose tissue from different fat depots before and after weight loss...
  21. doi request reprint The CB1 antagonist rimonabant decreases insulin hypersecretion in rat pancreatic islets
    Lisa Getty-Kaushik
    Obesity Research Center, Department of Medicine, Boston University School of Medicine, Boston, Massachusetts, USA
    Obesity (Silver Spring) 17:1856-60. 2009
    ..In contrast, it appears to decrease stimulated secretion in islets from lean animals but not in obese or GL-exposed islets...
  22. ncbi request reprint Effects of dietary medium-chain triglyceride on weight loss and insulin sensitivity in a group of moderately overweight free-living type 2 diabetic Chinese subjects
    Jian Rong Han
    Department of Medicine, Obesity Research Center, Boston University School of Medicine, Boston, MA 02118, USA
    Metabolism 56:985-91. 2007
    ..Collectively, our results suggest a link between moderate consumption of MCT and improved risk factors in moderately overweight humans in a low-cost, free-living setting...
  23. ncbi request reprint Glucose-dependent insulinotropic polypeptide modulates adipocyte lipolysis and reesterification
    Lisa Getty-Kaushik
    Obesity Research Center, Department of Molecular Medicine, Boston University School of Medicine, Boston, MA 02118, USA
    Obesity (Silver Spring) 14:1124-31. 2006
    ....
  24. pmc Beta-mecaptoethanol suppresses inflammation and induces adipogenic differentiation in 3T3-F442A murine preadipocytes
    Wen Guo
    Department of Medicine, Boston University School of Medicine, Boston, Massachusetts, United States of America
    PLoS ONE 7:e40958. 2012
    ....
  25. doi request reprint Dual role of proapoptotic BAD in insulin secretion and beta cell survival
    Nika N Danial
    Department of Pathology, Harvard Medical School, Dana Farber Cancer Institute, 44 Binney Street, Boston, Massachusetts 02115, USA
    Nat Med 14:144-53. 2008
    ..Furthermore, we show that BAD regulates the physiologic adaptation of beta cell mass during high-fat feeding. Our findings provide genetic proof of the bifunctional activities of BAD in both beta cell survival and insulin secretion...
  26. ncbi request reprint Metabolic partitioning of endogenous fatty acid in adipocytes
    Tong Wang
    Obesity Research Center, Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    Obes Res 11:880-7. 2003
    ..To develop an accurate new method to measure the partitioning of adipocyte endogenous fatty acids among different metabolic pathways, a critical step toward understanding the regulatory mechanism by which fat disposition is modulated...
  27. ncbi request reprint Potentiation of insulin secretion by phorbol esters is mediated by PKC-alpha and nPKC isoforms
    Gordon C Yaney
    Obesity Research Center, Evans Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    Am J Physiol Endocrinol Metab 283:E880-8. 2002
    ..Therefore, stimulation of insulin secretion by PMA, and presumably by endogenous diacylglycerol, involves the activation of PKC isoforms delta and/or mu, and also PKC-alpha...
  28. ncbi request reprint Glucose-dependent increase in mitochondrial membrane potential, but not cytoplasmic calcium, correlates with insulin secretion in single islet cells
    Emma Heart
    Department of Medicine, Obesity Research Center, Boston University School of Medicine, Boston, MA 02118, USA
    Am J Physiol Endocrinol Metab 290:E143-E148. 2006
    ....
  29. ncbi request reprint 3H-serotonin as a marker of oscillatory insulin secretion in clonal beta-cells (INS-1)
    Jude T Deeney
    The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, S 171 76, Stockholm, Sweden
    FEBS Lett 581:4080-4. 2007
    ..The use of 3H-serotonin provides a real-time indicator of exocytosis from populations of clonal insulin-secreting cells...
  30. ncbi request reprint Medium-chain oil reduces fat mass and down-regulates expression of adipogenic genes in rats
    Jianrong Han
    Obesity Research Center, Boston University School of Medicine, Boston, Massachusetts, USA
    Obes Res 11:734-44. 2003
    ..To test the hypothesis that adipose tissue could be one of the primary targets through which medium-chain fatty acids (MCFAs) exert their metabolic influence...
  31. pmc Glucose-6-phosphate isomerase deficiency results in mTOR activation, failed translocation of lipin 1α to the nucleus and hypersensitivity to glucose: Implications for the inherited glycolytic disease
    Jorge F Haller
    Department of Physiology and Biophysics, Boston University School of Medicine, USA
    Biochim Biophys Acta 1812:1393-402. 2011
    ..These results shed light on the mechanism underlying the pathologies associated with inherited GPI deficiency and the variability in the severity of the symptoms observed in these patients...
  32. ncbi request reprint Sulfonylureas rapidly cross phospholipid bilayer membranes by a free-diffusion mechanism
    Frits Kamp
    Department of Physiology and Biophysics, Boston University School of Medicine, Boston, Massachusetts, USA
    Diabetes 52:2526-31. 2003
    ..Thus, we now provide a molecular mechanism by which sulfonylureas can penetrate the plasma membrane and reach intracellular sites regulating exocytosis...
  33. pmc Glucagon-like peptide 1 and fatty acids amplify pulsatile insulin secretion from perifused rat islets
    Barbara A Cunningham
    Department of Biochemistry, Boston University Medical Center, Room 815, 650 Albany Street, Boston, MA 02118, U S A and The Obesity Research Center, Evans Department of Medicine, Boston University Medical Center, 650 Albany Street, Boston, MA 02118, U S A
    Biochem J 369:173-8. 2003
    ..These data show that the physiological incretin GLP-1 preserves and enhances normal pulsatile insulin secretion, which may be essential in proposed therapeutic uses of GLP-1 or its analogues...
  34. ncbi request reprint Succinate is a preferential metabolic stimulus-coupling signal for glucose-induced proinsulin biosynthesis translation
    Cristina Alarcon
    Pacific Northwest Research Institute, Seattle, Washington 98122, USA
    Diabetes 51:2496-504. 2002
    ..Thus, succinate and/or succinyl-CoA appear to be preferential metabolic stimulus-coupling factors for glucose-induced proinsulin biosynthesis translation...
  35. ncbi request reprint Hormone-sensitive lipase has a role in lipid signaling for insulin secretion but is nonessential for the incretin action of glucagon-like peptide 1
    Marie Line Peyot
    Molecular Nutrition Unit, Department of Nutrition, University of Montréal and the Centre Hospitalier de l Université de Montréal, Montreal, Quebec, Canada
    Diabetes 53:1733-42. 2004
    ..Our results support the view that the availability of endogenous FFA through HSL and an additional enzyme(s) is involved in providing lipid moieties for beta-cell signaling for secretion in response to glucose...
  36. ncbi request reprint The L-type voltage-gated Ca2+ channel is the Ca2+ sensor protein of stimulus-secretion coupling in pancreatic beta cells
    Michael Trus
    Department of Biological Chemistry, Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel
    Biochemistry 46:14461-7. 2007
    ..These results confirm that the Ca2+ channel is a constituent of the exocytotic complex [Wiser et al. (1999) PNAS 96, 248-253] and the putative Ca2+-sensor protein of release...
  37. ncbi request reprint Increased beta-oxidation in muscle cells enhances insulin-stimulated glucose metabolism and protects against fatty acid-induced insulin resistance despite intramyocellular lipid accumulation
    German Perdomo
    Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 279:27177-86. 2004
    ..We propose that accelerated beta-oxidation in muscle cells exerts an insulin-sensitizing effect independently of changes in intracellular lipid content...
  38. ncbi request reprint Octanoate attenuates adipogenesis in 3T3-L1 preadipocytes
    Jianrong Han
    Departments of Medicine and Biochemistry, Obesity Research Center, Boston University School of Medicine, Boston, MA 02118, USA
    J Nutr 132:904-10. 2002
    ..These results suggest that octanoate may block adipogenesis at least in part by its influence on the expression/activation of PPARgamma...
  39. ncbi request reprint Rapid flip-flop of oleic acid across the plasma membrane of adipocytes
    Frits Kamp
    Obesity Research Center, Boston Medical Center, Massachusetts 02118, USA
    J Biol Chem 278:7988-95. 2003
    ..Any postulated transport mechanism for facilitating translocation of fatty acid across the plasma membrane of adipocytes, including a protein transporter, would have to compete with the highly effective flip-flop mechanism...
  40. ncbi request reprint Reactive oxygen species as a signal in glucose-stimulated insulin secretion
    Jingbo Pi
    Endocrine Biology Program, The Hamner Institutes for Health Sciences, 6 Davis Dr, Research Triangle Park, NC 27709, USA
    Diabetes 56:1783-91. 2007
    ..Taken together, these findings suggest that H2O2 derived from glucose metabolism is one of the metabolic signals for insulin secretion, whereas oxidative stress may disturb its signaling function...

Research Grants31

  1. METABOLIC REGULATION OF INSULIN SECRETION
    BARBARA CORKEY; Fiscal Year: 2005
    ..We will determine whether physiological alterations in 02- or NADPH alter TRX or GSH activity or DYPM and whether separately altering TRX, GSH or voltage-sensitive K+- channel activity alters insulin secretion. ..
  2. METABOLIC SIGNAL TRANSDUCTION IN ADIPOCYTES
    BARBARA CORKEY; Fiscal Year: 2007
    ..These experiments could reverse the thrifty phenotype and possibly lend insight into the thrifty genotype and its role in the development of obesity. ..
  3. METABOLIC REGULATION OF INSULIN SECRETION
    BARBARA CORKEY; Fiscal Year: 2007
    ..These redox sensitive channels reportedly play a role in PM repolarization. We will also determine whether NADPH, TRX or GSH are the main cytosolic redox modulators of Kv channel activity. ..
  4. METABOLIC SIGNAL TRANSDUCTION IN ADIPOCYTES
    Barbara E Corkey; Fiscal Year: 2010
    ..These experiments could reverse the thrifty phenotype and possibly lend insight into the thrifty genotype and its role in the development of obesity. ..
  5. Lipid signal transduction /oscillatory insulin secretion
    BARBARA CORKEY; Fiscal Year: 2004
    ..Aim 7 will test mechanisms of secretory and electrical synchronization in the intact perfused pancreas and its relationship to islet and single cell studies. ..
  6. METABOLIC REGULATION OF INSULIN SECRETION
    BARBARA CORKEY; Fiscal Year: 2003
    ..31P-NMR analysis of samples taken at different times after stimulation of beta-cells by glucose will be used to identify the source of Pi. ..
  7. METABOLIC SIGNAL TRANSDUCTION IN ADIPOCYTES
    BARBARA CORKEY; Fiscal Year: 2005
    ..Which enzymes regulate intracellular partitioning of FFA? 3. Do LC- and malonyl-CoA exert metabolic control over FFA partitioning? 4. Do FFA or LC-CoA modulate mitochondrial energy efficiency or the activity of UCP? ..
  8. METABOLIC REGULATION OF INSULIN SECRETION
    Orian S Shirihai; Fiscal Year: 2010
    ..Support for the role of ss-cell in mediating hyper insulinemia and insulin resistance would lead to radically different strategies for the treatment of insulin resistance and Type 2 diabetes. ..