Qizhen Shi

Summary

Affiliation: Blood Research Institute
Country: USA

Publications

  1. pmc Intravascular recovery of VWF and FVIII following intraperitoneal injection and differences from intravenous and subcutaneous injection in mice
    Q Shi
    Department of Pediatrics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Haemophilia 18:639-46. 2012
  2. pmc Targeting FVIII expression to endothelial cells regenerates a releasable pool of FVIII and restores hemostasis in a mouse model of hemophilia A
    Qizhen Shi
    Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, WI, USA
    Blood 116:3049-57. 2010
  3. pmc Platelets as delivery systems for disease treatments
    Qizhen Shi
    Blood Research Institute, BloodCenter of Wisconsin, Departments of Pediatrics, Medical College of Wisconsin, Children s Hospital of Wisconsin, Milwaukee, USA
    Adv Drug Deliv Rev 62:1196-203. 2010
  4. pmc Syngeneic transplantation of hematopoietic stem cells that are genetically modified to express factor VIII in platelets restores hemostasis to hemophilia A mice with preexisting FVIII immunity
    Qizhen Shi
    Department of Pediatrics, Medical College of Wisconsin, Milwaukee, USA
    Blood 112:2713-21. 2008
  5. ncbi request reprint Lentivirus-mediated platelet-derived factor VIII gene therapy in murine haemophilia A
    Q Shi
    Department of Pediatrics, Medical College of Wisconsin Milwaukee, WI 53226, USA
    J Thromb Haemost 5:352-61. 2007
  6. pmc Factor VIII ectopically targeted to platelets is therapeutic in hemophilia A with high-titer inhibitory antibodies
    Qizhen Shi
    Blood Research Institute, BloodCenter of Wisconsin, Department of Pediatrics, Medical College of Wisconsin, and Children s Research Institute, Children s Hospital of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Clin Invest 116:1974-82. 2006
  7. pmc Factor IX ectopically expressed in platelets can be stored in alpha-granules and corrects the phenotype of hemophilia B mice
    Guowei Zhang
    Department of Pediatrics, Medical College of Wisconsin, Milwaukee, USA
    Blood 116:1235-43. 2010
  8. doi request reprint Platelet gene therapy corrects the hemophilic phenotype in immunocompromised hemophilia A mice transplanted with genetically manipulated human cord blood stem cells
    Qizhen Shi
    Department of Pediatrics, Medical College of Wisconsin
    Blood 123:395-403. 2014
  9. pmc Correction of murine Bernard-Soulier syndrome by lentivirus-mediated gene therapy
    Sachiko Kanaji
    Blood Research Institute, Blood Center of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Mol Ther 20:625-32. 2012
  10. pmc Platelet and endothelial expression of clotting factors for the treatment of hemophilia
    Robert R Montgomery
    Blood Research Institute of BloodCenter of Wisconsin and Medical College of Wisconsin, Milwaukee, WI, USA
    Thromb Res 129:S46-8. 2012

Collaborators

Detail Information

Publications19

  1. pmc Intravascular recovery of VWF and FVIII following intraperitoneal injection and differences from intravenous and subcutaneous injection in mice
    Q Shi
    Department of Pediatrics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Haemophilia 18:639-46. 2012
    ..These results demonstrate that both VWF and FVIII can be efficiently absorbed into the blood circulation following i.p., but not s.c. injection, indicating that i.p. administration could be an alternative route for VWF or FVIII infusion...
  2. pmc Targeting FVIII expression to endothelial cells regenerates a releasable pool of FVIII and restores hemostasis in a mouse model of hemophilia A
    Qizhen Shi
    Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, WI, USA
    Blood 116:3049-57. 2010
    ....
  3. pmc Platelets as delivery systems for disease treatments
    Qizhen Shi
    Blood Research Institute, BloodCenter of Wisconsin, Departments of Pediatrics, Medical College of Wisconsin, Children s Hospital of Wisconsin, Milwaukee, USA
    Adv Drug Deliv Rev 62:1196-203. 2010
    ..This paper reviews the genetic therapy for inherited bleeding disorders utilizing platelets as delivery system, with a particular focus on platelet-derived FVIII for hemophilia A treatment...
  4. pmc Syngeneic transplantation of hematopoietic stem cells that are genetically modified to express factor VIII in platelets restores hemostasis to hemophilia A mice with preexisting FVIII immunity
    Qizhen Shi
    Department of Pediatrics, Medical College of Wisconsin, Milwaukee, USA
    Blood 112:2713-21. 2008
    ....
  5. ncbi request reprint Lentivirus-mediated platelet-derived factor VIII gene therapy in murine haemophilia A
    Q Shi
    Department of Pediatrics, Medical College of Wisconsin Milwaukee, WI 53226, USA
    J Thromb Haemost 5:352-61. 2007
    ....
  6. pmc Factor VIII ectopically targeted to platelets is therapeutic in hemophilia A with high-titer inhibitory antibodies
    Qizhen Shi
    Blood Research Institute, BloodCenter of Wisconsin, Department of Pediatrics, Medical College of Wisconsin, and Children s Research Institute, Children s Hospital of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Clin Invest 116:1974-82. 2006
    ..Our results demonstrate that this approach to the targeted expression of FVIII in platelets has the potential to correct hemophilia A, even in the presence of inhibitory immune responses to infused FVIII...
  7. pmc Factor IX ectopically expressed in platelets can be stored in alpha-granules and corrects the phenotype of hemophilia B mice
    Guowei Zhang
    Department of Pediatrics, Medical College of Wisconsin, Milwaukee, USA
    Blood 116:1235-43. 2010
    ..Our studies suggest that targeting FIX expression to platelets could be a new gene therapy strategy for hemophilia B...
  8. doi request reprint Platelet gene therapy corrects the hemophilic phenotype in immunocompromised hemophilia A mice transplanted with genetically manipulated human cord blood stem cells
    Qizhen Shi
    Department of Pediatrics, Medical College of Wisconsin
    Blood 123:395-403. 2014
    ..We demonstrate, for the first time, the feasibility of 2bF8LV gene delivery to human hematopoietic stem cells to introduce FVIII expression in human platelets and that human platelet-derived FVIII can improve hemostasis in hemophilia A. ..
  9. pmc Correction of murine Bernard-Soulier syndrome by lentivirus-mediated gene therapy
    Sachiko Kanaji
    Blood Research Institute, Blood Center of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Mol Ther 20:625-32. 2012
    ..These results demonstrate that lentivirus-mediated gene transfer can provide sustained phenotypic correction of murine BSS, indicating that this approach may be a promising strategy for gene therapy of BSS patients...
  10. pmc Platelet and endothelial expression of clotting factors for the treatment of hemophilia
    Robert R Montgomery
    Blood Research Institute of BloodCenter of Wisconsin and Medical College of Wisconsin, Milwaukee, WI, USA
    Thromb Res 129:S46-8. 2012
    ..These approaches have been studied in murine models but will need further study before this approach can be attempted clinically...
  11. ncbi request reprint Induction of megakaryocytes to synthesize and store a releasable pool of human factor VIII
    D A Wilcox
    Department of Pediatrics, Medical College of Wisconsin, and Children s Hospital of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Thromb Haemost 1:2477-89. 2003
    ..These results suggest feasibility for the development of a locally inducible secretory pool of FVIII in platelets of patients with hemophilia A...
  12. pmc Platelet-targeted gene therapy with human factor VIII establishes haemostasis in dogs with haemophilia A
    Lily M Du
    1 Department of Pediatrics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA 2 Children s Research Institute, Children s Hospital of Wisconsin, Milwaukee, Wisconsin 53226, USA 3 MACC Fund Research Center, Milwaukee, Wisconsin 53226, USA
    Nat Commun 4:2773. 2013
    ..Thus, platelet FVIII may provide effective long-term control of bleeding in patients with haemophilia A. ..
  13. ncbi request reprint A conditional knockout mouse model reveals endothelial cells as the principal and possibly exclusive source of plasma factor VIII
    Scot A Fahs
    Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, WI
    Blood 123:3706-13. 2014
    ..Our results indicate that endothelial cells are the predominant, and possibly exclusive, source of plasma FVIII. ..
  14. ncbi request reprint Platelet gene therapy by lentiviral gene delivery to hematopoietic stem cells restores hemostasis and induces humoral immune tolerance in FIX(null) mice
    Yingyu Chen
    1 Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, Wisconsin, USA 2 Fujian Institute of Hematology, Fujian Provincial Key Laboratory of Hematology, Fujian Medical University Union Hospital, Fuzhou, Fujian, China
    Mol Ther 22:169-77. 2014
    ..These data suggest that platelet gene therapy can not only restore hemostasis but also induce immune tolerance in hemophilia B mice, indicating that this approach may be a promising strategy for gene therapy of hemophilia B in humans...
  15. pmc Alternative strategies for gene therapy of hemophilia
    Robert R Montgomery
    Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, WI, USA
    Hematology Am Soc Hematol Educ Program 2010:197-202. 2010
    ..A safe cure of hemophilia is still the desired goal, but many barriers must still be overcome...
  16. ncbi request reprint Regulated release of VWF and FVIII and the biologic implications
    S L Haberichter
    Department of Pediatrics, Medical College of Wisconsin and Blood Research Institute, Blood Center of Wisconsin, Milwaukee, Wisconsin 53201, USA
    Pediatr Blood Cancer 46:547-53. 2006
    ..The data presented indicate that regulated secretion of both proteins occurs only if there is endogenous synthesis of FVIII together with VWF...
  17. ncbi request reprint Targeting platelet GPIbalpha transgene expression to human megakaryocytes and forming a complete complex with endogenous GPIbbeta and GPIX
    Q Shi
    Department of Pediatrics, Medical College of Wisconsin, MACC Fund Research Center MFRC, Milwaukee, WI 53226, USA
    J Thromb Haemost 2:1989-97. 2004
    ..This strategy could potentially be utilized for gene therapy of BSS...
  18. ncbi request reprint Expression of human factor VIII under control of the platelet-specific alphaIIb promoter in megakaryocytic cell line as well as storage together with VWF
    Q Shi
    Department of Pediatrics, Medical College of Wisconsin, MACC Fund Research Center MFRC, 8701 Watertown Plank Road, Milwaukee 53226 0509, USA
    Mol Genet Metab 79:25-33. 2003
    ..The data indicate that the megakaryocytic compartment of hematopoietic cells may represent a potential target of gene therapy for hemophilia A-especially in those patients who have developed inhibitors to plasma FVIII...
  19. ncbi request reprint Factor VIII ectopically expressed in platelets: efficacy in hemophilia A treatment
    Helen V Yarovoi
    The Children s Hospital of Philadelphia, 1 Civic Center, ARC, Rm 317, Philadelphia, PA 19104, USA
    Blood 102:4006-13. 2003
    ..Our studies also suggest that platelet-released factor VIII is at least as potent as an equivalent plasma level and perhaps even more potent in an arterial thrombosis model...