Peter J Newman

Summary

Affiliation: Blood Research Institute
Country: USA

Publications

  1. ncbi request reprint PECAM-1 functions as a specific and potent inhibitor of mitochondrial-dependent apoptosis
    Cunji Gao
    Laboratories of PECAM 1 Research and Cell Biology, Blood Research Institute, The Blood Center of Southeastern Wisconsin, PO Box 2178, 638 N 18th St, Milwaukee, WI 53201, USA
    Blood 102:169-79. 2003
  2. ncbi request reprint Signal transduction pathways mediated by PECAM-1: new roles for an old molecule in platelet and vascular cell biology
    Peter J Newman
    Blood Research Institute, The Blood Center of Southeastern Wisconsin, PO Box 2178, 638 N 18th St, Milwaukee, Wis 53201, USA
    Arterioscler Thromb Vasc Biol 23:953-64. 2003
  3. pmc Integrin activation by regulated dimerization and oligomerization of platelet endothelial cell adhesion molecule (PECAM)-1 from within the cell
    T Zhao
    Blood Research Institute, The Blood Center of Southeastern Wisconsin, Milwaukee, Wisconsin 53201, USA
    J Cell Biol 152:65-73. 2001
  4. pmc Eptifibatide-induced thrombocytopenia and thrombosis in humans require FcgammaRIIa and the integrin beta3 cytoplasmic domain
    Cunji Gao
    Blood Research Institute, Blood Center of Wisconsin, 638 N 18th Street, Milwaukee, WI 53201, USA
    J Clin Invest 119:504-11. 2009
  5. ncbi request reprint The cell-adhesion and signaling molecule PECAM-1 is a molecular mediator of resistance to genotoxic chemotherapy
    Carmen Bergom
    Blood Research Institute, Blood Center of Wisconsin, and Department of Cell Biology, Medical College of Wisconsin, Milwaukee, Wisconsin 53201, USA
    Cancer Biol Ther 5:1699-707. 2006
  6. pmc Identification of FcgammaRIIa as the ITAM-bearing receptor mediating alphaIIbbeta3 outside-in integrin signaling in human platelets
    Brian Boylan
    Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, USA
    Blood 112:2780-6. 2008
  7. ncbi request reprint Reactive oxygen species induce reversible PECAM-1 tyrosine phosphorylation and SHP-2 binding
    Matthias Maas
    Blood Research Institute, The Blood Center of Southeastern Wisconsin, PO Box 2178, Milwaukee, WI 53201 2178, USA
    Am J Physiol Heart Circ Physiol 285:H2336-44. 2003
  8. pmc Immunoreceptor tyrosine-based inhibitory motif (ITIM)-mediated inhibitory signaling is regulated by sequential phosphorylation mediated by distinct nonreceptor tyrosine kinases: a case study involving PECAM-1
    Benjamin E Tourdot
    Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Biochemistry 52:2597-608. 2013
  9. pmc Cooperative integrin/ITAM signaling in platelets enhances thrombus formation in vitro and in vivo
    Huiying Zhi
    Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, WI 53201, USA
    Blood 121:1858-67. 2013
  10. pmc Drug-dependent clearance of human platelets in the NOD/scid mouse by antibodies from patients with drug-induced immune thrombocytopenia
    Daniel W Bougie
    Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, WI, USA
    Blood 116:3033-8. 2010

Collaborators

Detail Information

Publications44

  1. ncbi request reprint PECAM-1 functions as a specific and potent inhibitor of mitochondrial-dependent apoptosis
    Cunji Gao
    Laboratories of PECAM 1 Research and Cell Biology, Blood Research Institute, The Blood Center of Southeastern Wisconsin, PO Box 2178, 638 N 18th St, Milwaukee, WI 53201, USA
    Blood 102:169-79. 2003
    ....
  2. ncbi request reprint Signal transduction pathways mediated by PECAM-1: new roles for an old molecule in platelet and vascular cell biology
    Peter J Newman
    Blood Research Institute, The Blood Center of Southeastern Wisconsin, PO Box 2178, 638 N 18th St, Milwaukee, Wis 53201, USA
    Arterioscler Thromb Vasc Biol 23:953-64. 2003
    ....
  3. pmc Integrin activation by regulated dimerization and oligomerization of platelet endothelial cell adhesion molecule (PECAM)-1 from within the cell
    T Zhao
    Blood Research Institute, The Blood Center of Southeastern Wisconsin, Milwaukee, Wisconsin 53201, USA
    J Cell Biol 152:65-73. 2001
    ....
  4. pmc Eptifibatide-induced thrombocytopenia and thrombosis in humans require FcgammaRIIa and the integrin beta3 cytoplasmic domain
    Cunji Gao
    Blood Research Institute, Blood Center of Wisconsin, 638 N 18th Street, Milwaukee, WI 53201, USA
    J Clin Invest 119:504-11. 2009
    ....
  5. ncbi request reprint The cell-adhesion and signaling molecule PECAM-1 is a molecular mediator of resistance to genotoxic chemotherapy
    Carmen Bergom
    Blood Research Institute, Blood Center of Wisconsin, and Department of Cell Biology, Medical College of Wisconsin, Milwaukee, Wisconsin 53201, USA
    Cancer Biol Ther 5:1699-707. 2006
    ..In addition, reducing PECAM-1 levels in the tumor endothelium may aid in low-dose, anti-angiogenic therapy...
  6. pmc Identification of FcgammaRIIa as the ITAM-bearing receptor mediating alphaIIbbeta3 outside-in integrin signaling in human platelets
    Brian Boylan
    Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, USA
    Blood 112:2780-6. 2008
    ....
  7. ncbi request reprint Reactive oxygen species induce reversible PECAM-1 tyrosine phosphorylation and SHP-2 binding
    Matthias Maas
    Blood Research Institute, The Blood Center of Southeastern Wisconsin, PO Box 2178, Milwaukee, WI 53201 2178, USA
    Am J Physiol Heart Circ Physiol 285:H2336-44. 2003
    ..These findings support a role for PECAM-1 as a sensor of oxidative stress, perhaps most importantly during the process of inflammation...
  8. pmc Immunoreceptor tyrosine-based inhibitory motif (ITIM)-mediated inhibitory signaling is regulated by sequential phosphorylation mediated by distinct nonreceptor tyrosine kinases: a case study involving PECAM-1
    Benjamin E Tourdot
    Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Biochemistry 52:2597-608. 2013
    ....
  9. pmc Cooperative integrin/ITAM signaling in platelets enhances thrombus formation in vitro and in vivo
    Huiying Zhi
    Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, WI 53201, USA
    Blood 121:1858-67. 2013
    ....
  10. pmc Drug-dependent clearance of human platelets in the NOD/scid mouse by antibodies from patients with drug-induced immune thrombocytopenia
    Daniel W Bougie
    Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, WI, USA
    Blood 116:3033-8. 2010
    ....
  11. pmc Residues within a lipid-associated segment of the PECAM-1 cytoplasmic domain are susceptible to inducible, sequential phosphorylation
    Cathy Paddock
    Blood Research Institute, BloodCenter of Wisconsin and Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Blood 117:6012-23. 2011
    ..On the basis of these findings, we propose a novel model for regulation of inhibitory signaling by ITIM-containing receptors that relies on reversible plasma membrane interactions and sequential ITIM phosphorylation...
  12. pmc PECAM-1 dampens cytokine levels during LPS-induced endotoxemia by regulating leukocyte trafficking
    Jamie R Privratsky
    Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, WI 53201, United States
    Life Sci 90:177-84. 2012
    ..The purpose of the present investigation was to test the hypothesis that PECAM-1 influences circulating cytokine levels by regulating the trafficking of activated, cytokine-producing leukocytes to sites of inflammation...
  13. pmc Site-specific effects of PECAM-1 on atherosclerosis in LDL receptor-deficient mice
    Reema Goel
    Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, WI 53201, USA
    Arterioscler Thromb Vasc Biol 28:1996-2002. 2008
    ..PECAM-1 also inhibits inflammatory responses, on which basis it is predicted to be atheroprotective...
  14. ncbi request reprint Peroxynitrite reduces the endothelium-derived hyperpolarizing factor component of coronary flow-mediated dilation in PECAM-1-knockout mice
    Yanping Liu
    Cardiovascular Center, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Am J Physiol Regul Integr Comp Physiol 290:R57-65. 2006
    ..In the absence of this adhesion and signaling molecule, ONOO- production is increased concomitant with a reduction in both the EDHF and INDO-sensitive components of FMD...
  15. pmc PECAM-1: conflicts of interest in inflammation
    Jamie R Privratsky
    Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, WI 53201, USA
    Life Sci 87:69-82. 2010
    ..A better understanding of how inflammatory responses are regulated should enable the development of new therapeutics that can be used in the treatment of acute and chronic inflammatory disorders...
  16. pmc Heparin promotes platelet responsiveness by potentiating αIIbβ3-mediated outside-in signaling
    Cunji Gao
    Blood Research Institute, Medical College of Wisconsin, Milwaukee, WI, USA
    Blood 117:4946-52. 2011
    ....
  17. pmc Platelet hyperreactivity explains the bleeding abnormality and macrothrombocytopenia in a murine model of sitosterolemia
    Taisuke Kanaji
    Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, WI
    Blood 122:2732-42. 2013
    ..Intercalation of plant sterols into the plasma membrane therefore results in dysregulation of multiple platelet activation pathways, leading to macrothrombocytopenia and bleeding. ..
  18. pmc An alternatively spliced isoform of PECAM-1 is expressed at high levels in human and murine tissues, and suggests a novel role for the C-terminus of PECAM-1 in cytoprotective signaling
    Carmen Bergom
    Blood Research Institute, BloodCenter of Wisconsin, 8727 Watertown Plank Road, Milwaukee, WI 53201, USA
    J Cell Sci 121:1235-42. 2008
    ..These studies suggest a novel role for the PECAM-1 C-terminus in cytoprotective signaling and highlight a need for further characterization of expression of PECAM-1 isoforms in normal and malignant tissues...
  19. ncbi request reprint The proinflammatory phenotype of PECAM-1-deficient mice results in atherogenic diet-induced steatohepatitis
    Reema Goel
    Blood Research Institute, BloodCenter of Wisconsin, P O Box 2178, Milwaukee, WI 53201, USA
    Am J Physiol Gastrointest Liver Physiol 293:G1205-14. 2007
    ..Future examination of recently identified PECAM-1 allelic isoforms in humans as potential risk factors for developing NASH may be warranted...
  20. pmc Blockade of maternal anti-HPA-1a-mediated platelet clearance by an HPA-1a epitope-specific F(ab') in an in vivo mouse model of alloimmune thrombocytopenia
    Tamam Bakchoul
    Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, 53201, USA
    Transfusion 49:265-70. 2009
    ....
  21. pmc Paxillin family members function as Csk-binding proteins that regulate Lyn activity in human and murine platelets
    Vipul B Rathore
    Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, WI, USA
    Biochem J 403:275-81. 2007
    ..These observations support the notion that Hic-5 and paxillin function as negative feedback regulators of SFKs in aggregated platelets and that, when both are present, paxillin is preferentially used...
  22. ncbi request reprint Endothelial cell PECAM-1 confers protection against endotoxic shock
    Matthias Maas
    Blood Research Institute, Blood Center of Southeastern Wisconsin, PO Box 2178, 638 N 18th St, Milwaukee, WI 53201, USA
    Am J Physiol Heart Circ Physiol 288:H159-64. 2005
    ..The diverse functional roles played by PECAM-1 in thrombosis, inflammation, apoptosis, and the immune response may make this molecule an attractive target for the development of novel therapeutics to manage and treat endotoxic shock...
  23. pmc Activation-independent, antibody-mediated removal of GPVI from circulating human platelets: development of a novel NOD/SCID mouse model to evaluate the in vivo effectiveness of anti-human platelet agents
    Brian Boylan
    Blood Research Institute, BloodCenter of Wisconsin, PO Box 2178, 638 N 18th St, Milwaukee, 53201, USA
    Blood 108:908-14. 2006
    ....
  24. pmc Neutrophil proteinase 3 acts on protease-activated receptor-2 to enhance vascular endothelial cell barrier function
    Christopher J Kuckleburg
    Blood Research Institute, BloodCenter of Wisconsin, 8727 Watertown Plank Rd, Milwaukee, WI 53051, USA
    Arterioscler Thromb Vasc Biol 33:275-84. 2013
    ..We examined whether neutrophils might secrete factors that promote vascular integrity during the latter stages of neutrophil transmigration, in particular, the role of neutrophil serine proteinase 3 (PR3)...
  25. pmc Lyn and PECAM-1 function as interdependent inhibitors of platelet aggregation
    Zhangyin Ming
    Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, WI, USA
    Blood 117:3903-6. 2011
    ..These results support a model in which PECAM-1/SHP-2 complexes, formed in a Lyn-dependent manner, suppress GPVI signaling...
  26. pmc Relative contribution of PECAM-1 adhesion and signaling to the maintenance of vascular integrity
    Jamie R Privratsky
    Blood Research Institute, Blood Center of Wisconsin, Milwaukee, WI 53201, USA
    J Cell Sci 124:1477-85. 2011
    ..Taken together, these studies suggest that PECAM-1-PECAM-1 homophilic interactions are more important than its signaling function for maintaining the integrity of endothelial cell junctions...
  27. ncbi request reprint PECAM-1 negatively regulates GPIb/V/IX signaling in murine platelets
    Vipul Rathore
    Blood Research Institute, The Blood Center of Southeastern Wisconsin, PO Box 2178, 638 N 18th St, Milwaukee, WI 53201, USA
    Blood 102:3658-64. 2003
    ....
  28. ncbi request reprint Mechanisms of PECAM-1-mediated cytoprotection and implications for cancer cell survival
    Carmen Bergom
    Blood Research Institute, The Blood Center of Southeastern Wisconsin, Milwaukee, Milwaukee, WI, USA
    Leuk Lymphoma 46:1409-21. 2005
    ....
  29. ncbi request reprint Phospholipase Cgamma2 contributes to stable thrombus formation on VWF
    Vipul Rathore
    Blood Research Institute The Blood Center of Southeastern Wisconsin, P O Box 2178, 638 N 18th St, Milwaukee, WI 53201, USA
    FEBS Lett 573:26-30. 2004
    ..While absence of PLCgamma2 had only a minimal effect on platelet adhesion to immobilized VWF, its absence impaired spreading and profoundly affected thrombus growth and stability on VWF...
  30. pmc The anti-inflammatory actions of platelet endothelial cell adhesion molecule-1 do not involve regulation of endothelial cell NF-kappa B
    Jamie R Privratsky
    Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, WI 53201, USA
    J Immunol 184:3157-63. 2010
    ..Taken together, these studies strongly suggest that the anti-inflammatory actions of PECAM-1 in endothelial cells are not likely to involve its regulation of NF-kappaB...
  31. ncbi request reprint Anti-GPVI-associated ITP: an acquired platelet disorder caused by autoantibody-mediated clearance of the GPVI/FcRgamma-chain complex from the human platelet surface
    Brian Boylan
    Blood Research Institute, The Blood Center of Southeastern Wisconsin, PO Box 2178, 638 N 18th St, Milwaukee, WI 53201, USA
    Blood 104:1350-5. 2004
    ....
  32. ncbi request reprint Disruption of the long-range GPIIIa Cys(5)-Cys(435) disulfide bond results in the production of constitutively active GPIIb-IIIa (alpha(IIb)beta(3)) integrin complexes
    Qi hong Sun
    Blood Research Institute, Blood Center of Southeastern Wisconsin, 8727 Watertown Plank Road, PO Box 2178, Milwaukee, WI 53233, USA
    Blood 100:2094-101. 2002
    ..Additionally, these studies may provide a molecular explanation for the previously reported ability of mild reducing agents to activate the GPIIb-IIIa complex and promote platelet aggregation...
  33. ncbi request reprint A site involving the "hybrid" and PSI homology domains of GPIIIa (beta 3-integrin subunit) is a common target for antibodies associated with quinine-induced immune thrombocytopenia
    Julie A Peterson
    Blood Research Institute, The Blood Center of Southeastern Wisconsin, and the Department of Cellular Biology, Medical College of Wisconsin, Milwaukee 53201 2178, USA
    Blood 101:937-42. 2003
    ..The findings suggest this region of GPIIIa may be a favored target for quinine-dependent antibodies and may provide a basis for further studies to elucidate the molecular basis of glycoprotein-drug-antibody interaction...
  34. pmc Proteinase 3 contributes to transendothelial migration of NB1-positive neutrophils
    Christopher J Kuckleburg
    Blood Research Institute, Blood Center of Wisconsin, Milwaukee, WI 53201, USA
    J Immunol 188:2419-26. 2012
    ..These data demonstrate that PR3 contributes in the selective recruitment of the NB1-positive neutrophil population...
  35. ncbi request reprint Nitration of PECAM-1 ITIM tyrosines abrogates phosphorylation and SHP-2 binding
    Debra K Newman
    Blood Research Institute, The Blood Center of Southeastern Wisconsin, 53226, Milwaukee, WI, USA
    Biochem Biophys Res Commun 296:1171-9. 2002
    ..These data suggest that ITIM tyrosine nitration may represent a mechanism for modulating phosphotyrosine-dependent signal transduction pathways...
  36. pmc GPIbα regulates platelet size by controlling the subcellular localization of filamin
    Taisuke Kanaji
    Blood Research Institute, Blood Center of Wisconsin, Milwaukee, WI 53266 3548, USA
    Blood 119:2906-13. 2012
    ..Taken together, these data demonstrate that coordinated expression of GPIbα and filamin is required for efficient trafficking of either protein to the cell surface, and for production of normal-sized platelets...
  37. pmc The alleles of PECAM-1
    Melanie S Novinska
    Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, WI 53201, USA
    Gene 376:95-101. 2006
    ..Identification of these PECAM-1 allelic isoforms should facilitate future detailed examination of PECAM-1-related disease associations, and may help resolve previously disparate results...
  38. pmc PECAM-1: regulator of endothelial junctional integrity
    Jamie R Privratsky
    Blood Research Institute, BloodCenter of Wisconsin, P O Box 2178, 638N 18th Street, Milwaukee, WI, 53201, USA
    Cell Tissue Res 355:607-19. 2014
    ....
  39. ncbi request reprint Integrins: dynamic scaffolds for adhesion and signaling in platelets
    Sanford J Shattil
    Department of Cell Biology, The Scripps Research Institute, La Jolla, CA, USA
    Blood 104:1606-15. 2004
    ....
  40. ncbi request reprint The neutrophil-specific antigen CD177 is a counter-receptor for platelet endothelial cell adhesion molecule-1 (CD31)
    Ulrich J H Sachs
    Institute for Clinical Immunology and Transfusion Medicine, Justus Liebig University, Langhansstrasse 7, Giessen D 35392, Germany
    J Biol Chem 282:23603-12. 2007
    ..Our findings provide direct evidence that neutrophil-specific CD177 is a heterophilic binding partner of PECAM-1. This interaction may constitute a new pathway that participates in neutrophil transmigration...
  41. pmc Platelet PECAM-1 inhibits thrombus formation in vivo
    Shahrokh Falati
    Center for Hemostasis and Thrombosis Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA
    Blood 107:535-41. 2006
    ..In PECAM-1-deficient mice the time to 75% vessel occlusion was significantly shorter than in control mice. These data provide evidence for the involvement of platelet PECAM-1 in the negative regulation of thrombus formation...
  42. ncbi request reprint Palmitoylation at Cys595 is essential for PECAM-1 localisation into membrane microdomains and for efficient PECAM-1-mediated cytoprotection
    Caroline T Sardjono
    Kronheimer Building, Burnet Institute incorporating the Austin Research Institute, Studley Road, Heidelberg, Victoria, 3084 Australia
    Thromb Haemost 96:756-66. 2006
    ..These results show that cysteine 595 is required for constitutive association of PECAM-1 with membrane microdomains and PECAM-1-mediated cytoprotection, where it may act as a crucial regulator of signaling and apoptosis events...
  43. pmc Tests of the extension and deadbolt models of integrin activation
    Jieqing Zhu
    The CBR Institute for Biomedical Research, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 282:11914-20. 2007
    ..The data demonstrate that integrin extension is required for ligand binding during integrin inside-out signaling and that the deadbolt does not regulate integrin activation...
  44. pmc Mapping early conformational changes in alphaIIb and beta3 during biogenesis reveals a potential mechanism for alphaIIbbeta3 adopting its bent conformation
    W Beau Mitchell
    Department of Pediatrics, Mount Sinai School of Medicine, and New York Blood Center, 310 E 67th Street, New York, NY 10021, USA
    Blood 109:3725-32. 2007
    ..Our data suggest a working model in which pro-alphaIIb adopts a bent conformation soon after synthesis, and then beta3 assumes its bent conformation by virtue of its interaction with the bent pro-alphaIIb...