Ramunas Stepanauskas

Summary

Affiliation: Bigelow Laboratory for Ocean Sciences
Country: USA

Publications

  1. doi Single cell genomics: an individual look at microbes
    Ramunas Stepanauskas
    Bigelow Laboratory for Ocean Sciences, East Boothbay, ME 04544, United States
    Curr Opin Microbiol 15:613-20. 2012
  2. ncbi Coselection for microbial resistance to metals and antibiotics in freshwater microcosms
    Ramunas Stepanauskas
    University of Georgia, Savannah River Ecology Laboratory, Aiken, SC, USA
    Environ Microbiol 8:1510-4. 2006
  3. pmc Matching phylogeny and metabolism in the uncultured marine bacteria, one cell at a time
    Ramunas Stepanauskas
    Bigelow Laboratory for Ocean Sciences, P O Box 475, West Boothbay Harbor, ME 04575 0475, USA
    Proc Natl Acad Sci U S A 104:9052-7. 2007
  4. doi Influence of industrial contamination on mobile genetic elements: class 1 integron abundance and gene cassette structure in aquatic bacterial communities
    Meredith S Wright
    Savannah River Ecology Laboratory, University of Georgia, Aiken, SC, USA
    ISME J 2:417-28. 2008
  5. pmc Unveiling in situ interactions between marine protists and bacteria through single cell sequencing
    Manuel Martinez-Garcia
    Single Cell Genomics Center, Bigelow Laboratory for Ocean Sciences, West Boothbay Harbor, ME 04575 0475, USA
    ISME J 6:703-7. 2012
  6. ncbi Antibiotic resistance in the shellfish pathogen Vibrio parahaemolyticus isolated from the coastal water and sediment of Georgia and South Carolina, USA
    Craig Baker-Austin
    Savannah River Ecology Laboratory, Drawer E, Aiken, South Carolina 29802, USA
    J Food Prot 71:2552-8. 2008
  7. ncbi Elevated microbial tolerance to metals and antibiotics in metal-contaminated industrial environments
    Ramunas Stepanauskas
    Savannah River Ecology Laboratory, University of Georgia, Drawer E, Aiken, South Carolina 29802, USA
    Environ Sci Technol 39:3671-8. 2005
  8. doi Multi-site analysis reveals widespread antibiotic resistance in the marine pathogen Vibrio vulnificus
    Craig Baker-Austin
    Savannah River Ecology Laboratory, Drawer E, Aiken, SC, USA
    Microb Ecol 57:151-9. 2009
  9. pmc Prevalent genome streamlining and latitudinal divergence of planktonic bacteria in the surface ocean
    Brandon K Swan
    Bigelow Laboratory for Ocean Sciences, East Boothbay, ME 04544, USA
    Proc Natl Acad Sci U S A 110:11463-8. 2013
  10. pmc Capturing single cell genomes of active polysaccharide degraders: an unexpected contribution of Verrucomicrobia
    Manuel Martinez-Garcia
    Bigelow Laboratory for Ocean Sciences, West Boothbay Harbor, Main, United States of America
    PLoS ONE 7:e35314. 2012

Collaborators

Detail Information

Publications21

  1. doi Single cell genomics: an individual look at microbes
    Ramunas Stepanauskas
    Bigelow Laboratory for Ocean Sciences, East Boothbay, ME 04544, United States
    Curr Opin Microbiol 15:613-20. 2012
    ....
  2. ncbi Coselection for microbial resistance to metals and antibiotics in freshwater microcosms
    Ramunas Stepanauskas
    University of Georgia, Savannah River Ecology Laboratory, Aiken, SC, USA
    Environ Microbiol 8:1510-4. 2006
    ..Our results provide first experimental evidence that the exposure of freshwater environments to individual metals and antibiotics selects for multiresistant microorganisms, including opportunistic human pathogens...
  3. pmc Matching phylogeny and metabolism in the uncultured marine bacteria, one cell at a time
    Ramunas Stepanauskas
    Bigelow Laboratory for Ocean Sciences, P O Box 475, West Boothbay Harbor, ME 04575 0475, USA
    Proc Natl Acad Sci U S A 104:9052-7. 2007
    ..Compared with metagenomics, the power of our approach lies in the ability to detect metabolic genes in uncultured microorganisms directly, even when the metabolic and phylogenetic markers are located far apart on the chromosome...
  4. doi Influence of industrial contamination on mobile genetic elements: class 1 integron abundance and gene cassette structure in aquatic bacterial communities
    Meredith S Wright
    Savannah River Ecology Laboratory, University of Georgia, Aiken, SC, USA
    ISME J 2:417-28. 2008
    ....
  5. pmc Unveiling in situ interactions between marine protists and bacteria through single cell sequencing
    Manuel Martinez-Garcia
    Single Cell Genomics Center, Bigelow Laboratory for Ocean Sciences, West Boothbay Harbor, ME 04575 0475, USA
    ISME J 6:703-7. 2012
    ..This study demonstrates the power of single cell sequencing to untangle ecological interactions between uncultured protists and prokaryotes...
  6. ncbi Antibiotic resistance in the shellfish pathogen Vibrio parahaemolyticus isolated from the coastal water and sediment of Georgia and South Carolina, USA
    Craig Baker-Austin
    Savannah River Ecology Laboratory, Drawer E, Aiken, South Carolina 29802, USA
    J Food Prot 71:2552-8. 2008
    ..parahaemolyticus strains. The observed antibiotic susceptibility patterns suggest that current guidelines for the antibiotic treatment of non-cholerae Vibrio should be reevaluated and extended...
  7. ncbi Elevated microbial tolerance to metals and antibiotics in metal-contaminated industrial environments
    Ramunas Stepanauskas
    Savannah River Ecology Laboratory, University of Georgia, Drawer E, Aiken, South Carolina 29802, USA
    Environ Sci Technol 39:3671-8. 2005
    ....
  8. doi Multi-site analysis reveals widespread antibiotic resistance in the marine pathogen Vibrio vulnificus
    Craig Baker-Austin
    Savannah River Ecology Laboratory, Drawer E, Aiken, SC, USA
    Microb Ecol 57:151-9. 2009
    ....
  9. pmc Prevalent genome streamlining and latitudinal divergence of planktonic bacteria in the surface ocean
    Brandon K Swan
    Bigelow Laboratory for Ocean Sciences, East Boothbay, ME 04544, USA
    Proc Natl Acad Sci U S A 110:11463-8. 2013
    ..Our study demonstrates the utility of single cell genomics for gaining an improved understanding of the composition and dynamics of natural microbial assemblages. ..
  10. pmc Capturing single cell genomes of active polysaccharide degraders: an unexpected contribution of Verrucomicrobia
    Manuel Martinez-Garcia
    Bigelow Laboratory for Ocean Sciences, West Boothbay Harbor, Main, United States of America
    PLoS ONE 7:e35314. 2012
    ..The employed method offers a powerful tool to rapidly identify and recover discrete genomes of active players in polysaccharide degradation, without the need for cultivation...
  11. pmc High-throughput single-cell sequencing identifies photoheterotrophs and chemoautotrophs in freshwater bacterioplankton
    Manuel Martinez-Garcia
    Bigelow Laboratory for Ocean Sciences, West Boothbay Harbor, ME, USA
    ISME J 6:113-23. 2012
    ..This study demonstrates the power of single-cell DNA sequencing addressing previously unresolved questions about the metabolic potential and evolutionary histories of uncultured microorganisms, which dominate most natural environments...
  12. ncbi Bacterial tolerances to metals and antibiotics in metal-contaminated and reference streams
    Meredith S Wright
    University of Georgia, Savannah River Ecology Laboratory, Aiken, SC 29802, USA
    FEMS Microbiol Ecol 58:293-302. 2006
    ..Temporal dynamics were not directly correlated to a suite of physicochemical parameters, suggesting that tolerance patterns within microhabitats are linked to a complex interaction of the physicochemical characteristics of the stream...
  13. pmc Targeted sorting of single virus-infected cells of the coccolithophore Emiliania huxleyi
    Joaquin Martinez Martinez
    Bigelow Laboratory for Ocean Sciences, West Boothbay Harbor, Maine, United States of America
    PLoS ONE 6:e22520. 2011
    ..huxleyi. The method may be transferable to other virus-host systems...
  14. doi Single-cell genomics reveals organismal interactions in uncultivated marine protists
    Hwan Su Yoon
    Bigelow Laboratory for Ocean Sciences, West Boothbay Harbor, ME 04575, USA
    Science 332:714-7. 2011
    ..By using shotgun sequencing of uncultured marine picobiliphytes, we revealed the distinct interactions of individual cells...
  15. pmc Capturing diversity of marine heterotrophic protists: one cell at a time
    Jane L Heywood
    Bigelow Laboratory for Ocean Sciences, W Boothbay Harbor, ME, USA
    ISME J 5:674-84. 2011
    ..In addition, the creation of SAG libraries opens opportunities for the analysis of multiple genes or entire genomes of the uncultured protist groups...
  16. pmc What's new is old: resolving the identity of Leptothrix ochracea using single cell genomics, pyrosequencing and FISH
    Emily J Fleming
    Bigelow Laboratory for Ocean Sciences, West Boothbay Harbor, Maine, United States of America
    PLoS ONE 6:e17769. 2011
    ..The SSU rRNA gene of L. ochracea shares 96% homology with its closet cultivated relative, L. cholodnii, This establishes that L. ochracea is indeed related to this group of morphologically similar, filamentous, sheathed microorganisms...
  17. ncbi Co-selection of antibiotic and metal resistance
    Craig Baker-Austin
    Savannah River Ecology Laboratory, Drawer E, SC 29803, USA
    Trends Microbiol 14:176-82. 2006
    ....
  18. doi Potential for chemolithoautotrophy among ubiquitous bacteria lineages in the dark ocean
    Brandon K Swan
    Bigelow Laboratory for Ocean Sciences, 180 McKown Point Road, Post Office Box 475, West Boothbay Harbor, ME 04575, USA
    Science 333:1296-300. 2011
    ..Our study suggests potential chemolithoautotrophy in several uncultured Proteobacteria lineages that are ubiquitous in the dark oxygenated ocean and provides new perspective on carbon cycling in the ocean's largest habitat...
  19. pmc Flow-cytometric cell sorting and subsequent molecular analyses for culture-independent identification of bacterioplankton involved in dimethylsulfoniopropionate transformations
    Xiaozhen Mou
    Department of Marine Sciences, University of Georgia, Athens, GA 30602, USA
    Appl Environ Microbiol 71:1405-16. 2005
    ..Our study suggests that diverse bacterioplankton participate in the metabolism of DMSP in coastal marine systems and that their relative importance varies temporally...
  20. pmc Insights into the environmental resistance gene pool from the genome sequence of the multidrug-resistant environmental isolate Escherichia coli SMS-3-5
    W Florian Fricke
    Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Bacteriol 190:6779-94. 2008
    ..The association of antimicrobial resistance with APEC virulence genes on pSMS35_130 highlights the risk of promoting the spread of virulence through the extensive use of antibiotics...
  21. pmc Genomic sequencing of single microbial cells from environmental samples
    Thomas Ishoey
    J Craig Venter Institute, 10355 Science Center Drive, San Diego, CA 92121, United States
    Curr Opin Microbiol 11:198-204. 2008
    ..Methods are reviewed for preparation of bacterial fractions from environmental samples, single-cell isolation, DNA amplification by MDA, and DNA sequencing...