Genomes and Genes
G M Rubanyi
Affiliation: Berlex Biosciences
- Hedgehog-interacting protein is highly expressed in endothelial cells but down-regulated during angiogenesis and in several human tumorsCatherine L Olsen
Department of Gene Therapy, Berlex Laboratories, Inc, Richmond, CA 94806, USA
BMC Cancer 4:43. 2004..Recently a putative antagonist of the pathway, Hedgehog-interacting protein (HIP), was identified as a Hh binding protein that is also a target of Hh signaling. We sought to clarify possible roles for HIP in angiogenesis and cancer...
- Therapeutic angiogenesis and wound healing--IBC's International Conference. 15-16 November 2004, Cambridge, MA, USAGabor M Rubanyi
Berlex Biosciences, Vascular and Endothelial Research, 15049 San Pablo Avenue, PO Box 4099, Richmond, CA 94804 0099, USA
IDrugs 8:94-6. 2005
- Effect of estrogen on endothelial function and angiogenesisGabor M Rubanyi
Department of Gene Therapy, Berlex Biosciences, Richmond, CA, USA
Vascul Pharmacol 38:89-98. 2002..treatment of existing disease...
- Role of estrogen receptors in the vascular systemGabor M Rubanyi
Department of Gene Therapy, Berlex Biosciences, Richmond, CA, USA
Vascul Pharmacol 38:81-8. 2002..These studies in mice and a male human subject suggest that absence of functional ER may represent a novel risk factor for cardiovascular diseases...
- The future of human gene therapyG M Rubanyi
Gene Therapy Department, Berlex Biosciences, 15049 San Pablo Avenue, Richmond, CA 94804 0099, USA
Mol Aspects Med 22:113-42. 2001..The technical advances together with the ever increasing knowledge and experience in the field will undoubtedly lead to the realization of the full potential of HGT in the future...
- Endothelium-dependent vasorelaxation in the aorta of transgenic mice expressing human apolipoprotein(a) or lipoprotein(a)G M Rubanyi
Gene Therapy and Genomics Department, Berlex Biosciences Richmond, Californtia 94804, USA
Endothelium 7:253-64. 2000..These results show for the first time that elevated plasma levels of apo(a) and Lp(a) do not cause endothelial dysfunction in transgenic mice...
- Vascular estrogen receptors and endothelium-derived nitric oxide production in the mouse aorta. Gender difference and effect of estrogen receptor gene disruptionG M Rubanyi
Department of Cardiovascular Research, Berlex Biosciences, Richmond, California 94804 0099, USA
J Clin Invest 99:2429-37. 1997....
- Role of endogenous nitric oxide in progression of atherosclerosis in apolipoprotein E-deficient miceK Kauser
Cardiovascular Research, Berlex Biosciences, Richmond, California 94804 0099, USA
Am J Physiol Heart Circ Physiol 278:H1679-85. 2000..The acceleration in lesion size concomitant to the severely impaired NO-mediated responses indicates that lack of endogenous NO is an important progression factor of atherosclerosis in the apoE-KO mouse...
- Gender difference in endothelial dysfunction in the aorta of spontaneously hypertensive ratsK Kauser
Berlex Biosciences, Cardiovascular Department, Richmond, Calif 94804 0099, USA
Hypertension 25:517-23. 1995..These results suggest that sex steroid hormones may control endothelium-dependent vascular reactivity.(ABSTRACT TRUNCATED AT 250 WORDS)..
- Increased aortic stiffness assessed by pulse wave velocity in apolipoprotein E-deficient miceY X Wang
Department of Pharmacology, Berlex Biosciences, Richmond, California 94804, USA
Am J Physiol Heart Circ Physiol 278:H428-34. 2000..These results demonstrate for the first time that aortic stiffness determined by PWV increases in 13-mo-old apoE-KO mice. Endothelial dysfunction and elastic destruction in vascular wall caused by atherosclerosis may have contributed...
- Expression of interleukin-6 in atherosclerotic lesions of male ApoE-knockout mice: inhibition by 17beta-estradiolD A Sukovich
Department of Cardiovascular Research, Berlex Biosciences, Richmond, Calif 94804 0099, USA
Arterioscler Thromb Vasc Biol 18:1498-505. 1998....
- Effective treatment of vascular endothelial growth factor refractory hindlimb ischemia by a mutant endothelial nitric oxide synthase geneH S Qian
Department of Pharmacology, Berlex Biosciences, Richmond, CA, USA
Gene Ther 13:1342-50. 2006....
- Regulated expression of the interferon-beta gene in miceR N Harkins
Department of Gene Technologies, Berlex Biosciences, Richmond, CA, USA
Gene Ther 15:1-11. 2008....
- Development and validation of a robust and versatile one-plasmid regulated gene expression systemPaul Szymanski
Department of Gene Technologies, Berlex Biosciences, Richmond, California, USA
Mol Ther 15:1340-7. 2007..Finally, a pBRES expression cassette was transferred to an adeno-associated virus (AAV) vector and shown to be capable of regulated gene expression in vivo for nearly 1 year...
- Age-dependent acceleration of ischemic injury in endothelial nitric oxide synthase-deficient mice: potential role of impaired VEGF receptor 2 expressionHu Sheng Qian
Pharmacology Department, Berlex Biosciences, Richmond, CA, USA
J Cardiovasc Pharmacol 47:587-93. 2006..Lack of increased KDR expression may provide a mechanistic explanation for the severe ischemic injury and perhaps can be used as a clinical marker to identify severe, vascular endothelial growth factor refractory patient population...
- Gene expression profiling of human aortic endothelial cells exposed to disturbed flow and steady laminar flowAlan R Brooks
Gene Therapy Research Department, Berlex Biosciences, Richmond, California 94804, USA
Physiol Genomics 9:27-41. 2002..These studies demonstrate extensive and pathophysiologically relevant changes in sustained gene expression patterns in aortic EC exposed to DF compared with LF which are predicted to induce a proatherogenic EC phenotype...
- Transcriptional silencing is associated with extensive methylation of the CMV promoter following adenoviral gene delivery to muscleAlan R Brooks
Department of Gene Therapy, Berlex Biosciences, Richmond, CA 94804, USA
J Gene Med 6:395-404. 2004..Although the transient nature of transgene expression using first-generation adenovirus (Ad) vectors is well known, the exact mechanisms responsible for this phenomenon are uncertain...
- Gene expression profiling of vascular endothelial cells exposed to fluid mechanical forces: relevance for focal susceptibility to atherosclerosisAlan R Brooks
Gene Therapy Research Department, Berlex Biosciences, Richmond, California 94804 4099, USA
Endothelium 11:45-57. 2004....
- Mx1 and IP-10: biomarkers to measure IFN-beta activity in mice following gene-based deliveryHarald Petry
Department of Gene Therapy, Berlex Biosciences, Richmond, CA 94806, USA
J Interferon Cytokine Res 26:699-705. 2006....
- Accelerated atherosclerosis and premature calcified cartilaginous metaplasia in the aorta of diabetic male Apo E knockout mice can be prevented by chronic treatment with 17 beta-estradiolJ Tse
Berlex Biosciences, Department of Pharmacology, Richmond, CA 94804 0099, USA
Atherosclerosis 144:303-13. 1999....
- Identification of selective estrogen receptor modulators by their gene expression fingerprintsD A Zajchowski
Department of Cancer Research, Berlex Biosciences, Richmond, California 94804, USA
J Biol Chem 275:15885-94. 2000..Most compounds with similar GEFs had similar in vivo activities, thereby suggesting that GEF-based screens could be useful in predicting a compound's in vivo pharmacological profile...
- The role of endothelium in cardiovascular homeostasis and diseasesG M Rubanyi
Berlex Biosciences, Richmond, California 94804
J Cardiovasc Pharmacol 22:S1-14. 1993..Further knowledge of the pathobiology of the impaired endothelium will contribute to unraveling some of the remaining mysteries of many cardiovascular diseases and will enable us to design novel therapies to prevent and treat them...
- Synergistic effect of angiotensin II and nitric oxide synthase inhibitor in increasing aortic stiffness in miceRichard M Fitch
Department of Pharmacology, Berlex Biosciences, 2600 Hilltop Dr, Richmond, California 94804 0099, USA
Am J Physiol Heart Circ Physiol 290:H1190-8. 2006..These novel results suggest that NO deficiency and ANG II act synergistically to increase aortic stiffness in mice predominantly via changes in aortic wall collagen/elastin ratio...
- Gene-based delivery of IFN-beta is efficacious in a murine model of experimental allergic encephalomyelitisCaralee Schaefer
Department of Pharmacology, Berlex Biosciences, Richmond, CA 94804, USA
J Interferon Cytokine Res 26:449-54. 2006..A single injection of the MuIFN-beta plasmid was as effective in reducing the severity of the disease as an every other day injection of MuIFN-beta protein...
- Increased regional function and perfusion after intracoronary delivery of adenovirus encoding fibroblast growth factor 4: report of preclinical dataMei Hua Gao
VA San Diego Healthcare System 111 A, 3350 La Jolla Village Drive, San Diego, CA 92161, USA
Hum Gene Ther 15:574-87. 2004..Based on these preclinical studies, the method does not appear to be associated with major toxic effects...
- Fibroblast growth factor 4 induces vascular permeability, angiogenesis and arteriogenesis in a rabbit hindlimb ischemia modelTuomas T Rissanen
Department of Molecular Medicine, A I Virtanen Institute, Kuopio University, Finland
FASEB J 17:100-2. 2003..This study demonstrates for the first time that FGF-4 induces vascular permeability, therapeutic angiogenesis, and arteriogenesis comparable to that of VEGF and could be useful for the treatment of peripheral vascular disease...
- Endothelial nitric oxide synthase is critical for ischemic remodeling, mural cell recruitment, and blood flow reserveJun Yu
Department of Pharmacology and Vascular Cell Signaling and Therapeutics Program, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, CT 06536, USA
Proc Natl Acad Sci U S A 102:10999-1004. 2005..Collectively, our data suggest that endogenous eNOS-derived NO exerts direct effects in preserving blood flow, thereby promoting arteriogenesis, angiogenesis, and mural cell recruitment to immature angiogenic sprouts...
- Angiogenic gene therapy with adenovirus 5 fibroblast growth factor-4 (Ad5FGF-4): a new option for the treatment of coronary artery diseaseCindy Grines
William Beaumont Hospital, Royal Oak, Michigan 48073, USA
Am J Cardiol 92:24N-31N. 2003..FGF-4 was not detected in venous blood. Larger clinical trials will assess Ad5FGF-4 with regard to cardiovascular prognosis, symptom relief, and safety profile in patients with chronic stable angina...
- Estrogen inhibits the initiation of fatty streaks throughout the vasculature but does not inhibit intra-plaque hemorrhage and the progression of established lesions in apolipoprotein E deficient miceMichael E Rosenfeld
Department of Pathology, University of Washington, Seattle, WA 98195, USA
Atherosclerosis 164:251-9. 2002..These data suggest that estrogen inhibits the initiation of the fatty streak but does not alter the progression of established lesions through stages of instability and healing...