Holden T Maecker

Summary

Affiliation: BD Biosciences
Country: USA

Publications

  1. ncbi request reprint Functional T cell responses to tumor antigens in breast cancer patients have a distinct phenotype and cytokine signature
    Margaret Inokuma
    BD Biosciences, San Jose, CA 95131, USA
    J Immunol 179:2627-33. 2007
  2. pmc Results and harmonization guidelines from two large-scale international Elispot proficiency panels conducted by the Cancer Vaccine Consortium (CVC/SVI)
    Sylvia Janetzki
    ZellNet Consulting, Inc, Fort Lee, NJ, USA
    Cancer Immunol Immunother 57:303-15. 2008
  3. ncbi request reprint Flow cytometry controls, instrument setup, and the determination of positivity
    Holden T Maecker
    BD Biosciences, San Jose, CA 95131, USA
    Cytometry A 69:1037-42. 2006
  4. ncbi request reprint Selecting fluorochrome conjugates for maximum sensitivity
    Holden T Maecker
    BD Biosciences, San Jose, California 95131, USA
    Cytometry A 62:169-73. 2004
  5. ncbi request reprint T cell immunity to HIV: defining parameters of protection
    Holden T Maecker
    BD Biosciences, Immunocytometry Systems, 2350 Qume Drive, San Jose, CA 95131, USA
    Curr HIV Res 1:249-59. 2003
  6. pmc Human CD81 directly enhances Th1 and Th2 cell activation, but preferentially induces proliferation of Th2 cells upon long-term stimulation
    Holden T Maecker
    BD Biosciences, Immunocytometry Systems, 2350 Qume Drive, San Jose, CA 95131, USA
    BMC Immunol 4:1. 2003
  7. pmc Standardization of cytokine flow cytometry assays
    Holden T Maecker
    BD Biosciences, San Jose, USA
    BMC Immunol 6:13. 2005
  8. pmc Impact of cryopreservation on tetramer, cytokine flow cytometry, and ELISPOT
    Holden T Maecker
    BD Biosciences, San Jose, USA
    BMC Immunol 6:17. 2005
  9. ncbi request reprint Optimal preparation of rhesus macaque blood for cytokine flow cytometric analysis
    Laurel E Nomura
    Biological Research and Development, Immunocytometry Systems, BD Biosciences, 2350 Qume Drive, San Jose, CA 95131, USA
    Cytometry A 53:28-38. 2003
  10. ncbi request reprint Analyzing T-cell responses to cytomegalovirus by cytokine flow cytometry
    Holden T Maecker
    BD Biosciences, San Jose, California 95131, USA
    Hum Immunol 65:493-9. 2004

Collaborators

Detail Information

Publications34

  1. ncbi request reprint Functional T cell responses to tumor antigens in breast cancer patients have a distinct phenotype and cytokine signature
    Margaret Inokuma
    BD Biosciences, San Jose, CA 95131, USA
    J Immunol 179:2627-33. 2007
    ..Immunotherapies directed against TAAs may need to alter these T cell signatures to be effective...
  2. pmc Results and harmonization guidelines from two large-scale international Elispot proficiency panels conducted by the Cancer Vaccine Consortium (CVC/SVI)
    Sylvia Janetzki
    ZellNet Consulting, Inc, Fort Lee, NJ, USA
    Cancer Immunol Immunother 57:303-15. 2008
    ..These results provide initial harmonization guidelines to optimize Elispot assay performance to the immunotherapy community. Further optimization is in process with ongoing panels...
  3. ncbi request reprint Flow cytometry controls, instrument setup, and the determination of positivity
    Holden T Maecker
    BD Biosciences, San Jose, CA 95131, USA
    Cytometry A 69:1037-42. 2006
    ..In this tutorial, we classify controls in various categories, describe the options within each category, and discuss the merits of each option...
  4. ncbi request reprint Selecting fluorochrome conjugates for maximum sensitivity
    Holden T Maecker
    BD Biosciences, San Jose, California 95131, USA
    Cytometry A 62:169-73. 2004
  5. ncbi request reprint T cell immunity to HIV: defining parameters of protection
    Holden T Maecker
    BD Biosciences, Immunocytometry Systems, 2350 Qume Drive, San Jose, CA 95131, USA
    Curr HIV Res 1:249-59. 2003
    ..These parameters may include functional avidity, epitope breadth and specificity, proliferative capacity, cytokine repertoire, degree of anergy, and differentiation phenotype, as well as magnitude, of HIV-specific CD4 and CD8 T cells...
  6. pmc Human CD81 directly enhances Th1 and Th2 cell activation, but preferentially induces proliferation of Th2 cells upon long-term stimulation
    Holden T Maecker
    BD Biosciences, Immunocytometry Systems, 2350 Qume Drive, San Jose, CA 95131, USA
    BMC Immunol 4:1. 2003
    ..CD81, a cell-surface protein of the tetraspanin superfamily, has been shown to costimulate T cell activation in murine T cells, and is involved in development of Th2 immune responses in mice...
  7. pmc Standardization of cytokine flow cytometry assays
    Holden T Maecker
    BD Biosciences, San Jose, USA
    BMC Immunol 6:13. 2005
    ....
  8. pmc Impact of cryopreservation on tetramer, cytokine flow cytometry, and ELISPOT
    Holden T Maecker
    BD Biosciences, San Jose, USA
    BMC Immunol 6:17. 2005
    ..In this study, we compared the performance of these assays on leukapheresed PBMC shipped overnight in medium versus cryopreserved PBMC from matched donors...
  9. ncbi request reprint Optimal preparation of rhesus macaque blood for cytokine flow cytometric analysis
    Laurel E Nomura
    Biological Research and Development, Immunocytometry Systems, BD Biosciences, 2350 Qume Drive, San Jose, CA 95131, USA
    Cytometry A 53:28-38. 2003
    ..Monoclonal antibodies and fluorochromes optimized for use in macaques were included in samples examined for immune responses with the use of intracellular cytokine flow cytometry (CFC)...
  10. ncbi request reprint Analyzing T-cell responses to cytomegalovirus by cytokine flow cytometry
    Holden T Maecker
    BD Biosciences, San Jose, California 95131, USA
    Hum Immunol 65:493-9. 2004
    ..These data will be reviewed in terms of what they suggest about the threshold of protective T-cell immunity to CMV, and other factors in addition to T-cell frequencies that could be important in protecting from CMV-associated disease...
  11. pmc Precision and linearity targets for validation of an IFNgamma ELISPOT, cytokine flow cytometry, and tetramer assay using CMV peptides
    Holden T Maecker
    BD Biosciences, San Jose, CA, USA
    BMC Immunol 9:9. 2008
    ..Here we assess the levels of intra-assay, inter-assay, and inter-operator precision, as well as linearity, of CD8+ T cell IFNgamma-based ELISPOT and cytokine flow cytometry (CFC), as well as tetramer assays...
  12. pmc Performance of plate-based cytokine flow cytometry with automated data analysis
    Maria A Suni
    BD Biosciences, 2350 Qume Drive, San Jose, CA 95131, USA
    BMC Immunol 4:9. 2003
    ..To increase the throughput of CFC assays, we have optimized methods for stimulating, staining, and acquiring whole blood or PBMC samples in 96-well or 24-well plates...
  13. doi request reprint Standardization and optimization of multiparameter intracellular cytokine staining
    Laurel Nomura
    BD Biosciences, 2350 Qume Dr, San Jose, California 95131, USA
    Cytometry A 73:984-91. 2008
    ..We present data from two different ongoing multi-laboratory standardization studies, one involving 15 laboratories and one involving nine. We identify issues of variability and, where possible, offer solutions...
  14. pmc Quality assurance of intracellular cytokine staining assays: analysis of multiple rounds of proficiency testing
    Maria C Jaimes
    BD Biosciences, 2350 Qume Drive, San Jose, CA 95131, USA
    J Immunol Methods 363:143-57. 2011
    ....
  15. ncbi request reprint Dynamics of CD4 and CD8 T cell responses to cytomegalovirus in healthy human donors
    Holli S Dunn
    BD Biosciences, Immunocytometry Systems, San Jose, CA 95131, USA
    J Infect Dis 186:15-22. 2002
    ..By understanding the normal dynamics of CMV responses over time, it may be possible to better identify aberrant responses to CMV in immunocompromised hosts...
  16. ncbi request reprint Cytokine flow cytometry: a multiparametric approach for assessing cellular immune responses to viral antigens
    Vernon C Maino
    BD Biosciences, San Jose, CA 95131, USA
    Clin Immunol 110:222-31. 2004
    ..These ideas are discussed in the context of new developments in sample preparation and analysis automation...
  17. ncbi request reprint Ex vivo analysis of T-cell function
    Maria A Suni
    Becton, Dickinson and Company Biosciences, 2350 Qume Drive, San Jose, California 95131, USA
    Curr Opin Immunol 17:434-40. 2005
    ..Major recent developments in this area include the ability to measure T-cell degranulation via cell surface exposure of CD107 and the use of polychromatic flow cytometry to examine multiple phenotypes and functions of responding T cells...
  18. ncbi request reprint Cytokine flow cytometry
    Holden T Maecker
    Immunocytometry Systems, BD Biosciences, San Jose, CA, USA
    Methods Mol Biol 263:95-108. 2004
    ..In this chapter, specific methodology for such intracellular staining is elaborated, with emphasis on the effects of variables such as sample type, antigens, activation conditions, sample processing, and data acquisition and analysis...
  19. ncbi request reprint Analysis of the frequencies and of the memory T cell phenotypes of human CD8+ T cells specific for influenza A viruses
    Xiao Song He
    Stanford University School of Medicine, Stanford, California, USA
    J Infect Dis 187:1075-84. 2003
    ..The different patterns of CD27 expression in influenza virus- and cytomegalovirus-specific CD8+ T cells suggest that influenza virus-specific memory and effector CD8+ T cells can be differentiated by phenotypic analysis...
  20. doi request reprint An analytical workflow for investigating cytokine profiles
    Janet C Siebert
    CytoAnalytics, Analytical Services, Denver, Colorado 80209, USA
    Cytometry A 73:289-98. 2008
    ..In conclusion, the analytical workflow described herein yielded statistically supported and biologically relevant findings that were otherwise unapparent...
  21. ncbi request reprint Results of a cytomegalovirus (CMV)-specific CD8+/interferon- gamma+ cytokine flow cytometry assay correlate with clinical evidence of protective immunity in patients with AIDS with CMV retinitis
    Mark A Jacobson
    Positive Health Program, Department of Medicine, and San Francisco General Hospital General Clinical Research Center, University of California San Francisco, San Francisco, California, USA
    J Infect Dis 189:1362-73. 2004
    ..These data suggest that further testing of the CMV-specific CD8+/IFN- gamma+ CFC assay, for clinical utility in predicting incident and progressive CMVR disease, is warranted...
  22. pmc Poor predictive value of cytomegalovirus (CMV)-specific T cell assays for the development of CMV retinitis in patients with AIDS
    Mark A Jacobson
    Positive Health Program, Department of Medicine, University of California San Francisco, CA, USA
    Clin Infect Dis 46:458-66. 2008
    ..We examined the potential clinical utility of using a cytomegalovirus (CMV)-specific T cell immunoassay to determine the risk of developing new-onset CMV retinitis (CMVR) in patients with acquired immunodeficiency syndrome (AIDS)...
  23. pmc Development and dynamics of robust T-cell responses to CML under imatinib treatment
    Christiane I U Chen
    Department of Medicine, Division of Hematology, Stanford University School of Medicine, Stanford, CA 94305, USA
    Blood 111:5342-9. 2008
    ..This confirms the immune system's ability to respond to leukemia under certain conditions. Such responses may be further amplified as a potential therapy that synergizes with imatinib for improved control of CML...
  24. ncbi request reprint Epigenetic changes in tumor Fas levels determine immune escape and response to therapy
    Heather L Maecker
    Department of Radiation Oncology, Stanford University Medical Center, CCSR South, 269 Campus Drive, Stanford, California 94305, USA
    Cancer Cell 2:139-48. 2002
    ..This work demonstrates the importance of epigenetic modification of Fas in tumor progression and immune evasion, and emphasizes the essential interplay between Fas and innate immunity in the control of chemoresistant tumors...
  25. ncbi request reprint Protective immunity to cytomegalovirus (CMV) retinitis in AIDS is associated with CMV-specific T cells that express interferon- gamma and interleukin-2 and have a CD8+ cell early maturational phenotype
    Elizabeth Sinclair
    Division of Experimental Medicine, University of California, San Francisco, CA, USA
    J Infect Dis 194:1537-46. 2006
    ..Recovery from AIDS-related CMVR after the initiation of antiretroviral therapy may be mediated by CMV-specific CD4(+) and CD8(+) T cells capable of promoting antigen-specific CD8(+) T cell proliferation...
  26. ncbi request reprint TNF-alpha detection using a flow cytometric assay to determine cellular responses to anthrax vaccine
    Annie H Shinn
    Thomas J Long School of Pharmacy, University of the Pacific, Stockton, CA 95211, USA
    J Immunol Methods 282:169-74. 2003
    ..5 microg/ml and IFN-gamma production was not detected. This assay will be used in future larger prospective studies to further evaluate the cellular immune responses induced by the anthrax vaccine...
  27. ncbi request reprint Maximizing the retention of antigen specific lymphocyte function after cryopreservation
    Mary L Disis
    Tumor Vaccine Group, Center for Translational Medicine in Women s Health, 815 Mercer Street, 2nd Floor, Box 358050, University of Washington, Seattle, WA 98195 8050, USA
    J Immunol Methods 308:13-8. 2006
    ..I.) of the non-cryopreserved PBMC and SI obtained from cells frozen with media containing human serum albumin as compared to other additives such as dextran or fetal bovine serum...
  28. ncbi request reprint Strong cell-mediated immune responses are associated with the maintenance of low-level viremia in antiretroviral-treated individuals with drug-resistant human immunodeficiency virus type 1
    Steven G Deeks
    Department of Medicine, University of California, San Francisco, San Francisco, California, USA
    J Infect Dis 189:312-21. 2004
    ..Collectively, these data suggest that the selection of drug-resistance mutations may reduce the pathogenic potential of HIV, which leads to a balanced state of enhanced cellular immunity and low-level viremia...
  29. ncbi request reprint Persistent and selective deficiency of CD4+ T cell immunity to cytomegalovirus in immunocompetent young children
    Wenwei Tu
    Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305, USA
    J Immunol 172:3260-7. 2004
    ..Thus, CD4(+) T cell-mediated immunity to CMV in humans is generated in an age-dependent manner, and may have a substantial role in controlling renal viral replication and urinary shedding...
  30. ncbi request reprint Multicolor flow cytometric analysis in SIV-infected rhesus macaque
    Joshua M Walker
    Vaccine and Gene Therapy Institute, Oregon National Primate Research Center, Oregon Health and Science University, West Campus Beaverton, Oregon 97006, USA
    Methods Cell Biol 75:535-57. 2004
  31. ncbi request reprint Dysfunction of simian immunodeficiency virus/simian human immunodeficiency virus-induced IL-2 expression by central memory CD4+ T lymphocytes
    Yue Sun
    Division of Viral Pathogenesis, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA
    J Immunol 174:4753-60. 2005
    ..Persisting clinical protection in vaccinated and challenged monkeys is thus correlated with a preserved capacity of the peripheral blood central memory CD4+ T cells to express this important immunomodulatory cytokine...
  32. ncbi request reprint Immune responses in the draining lymph nodes against cancer: implications for immunotherapy
    Suyu Shu
    Center for Surgery Research, The Cleveland Clinic Foundation, Cleveland, OH, USA
    Cancer Metastasis Rev 25:233-42. 2006
    ..A clinical trial is now being carried out to test the immunogenicity and therapeutic effects of fusion hybrids for the treatment of metastatic melanoma...
  33. ncbi request reprint Immune dysfunction and micrometastases in women with breast cancer
    Michael J Campbell
    Department of Surgery, University of California, San Francisco, CA 94115, USA
    Breast Cancer Res Treat 91:163-71. 2005
    ..These dysregulated T cell responses did not correlate with age, stage of disease, or nodal status. However, we did observe a correlation between number of micrometastases in the bone marrow and T cell responsiveness...
  34. ncbi request reprint Increased brain size and glial cell number in CD81-null mice
    Eldon E Geisert
    Department of Anatomy and Neurobiology, and Neuroscience Institute, University of Tennessee, Health Science Center, Memphis, Tennessee 38163, USA
    J Comp Neurol 453:22-32. 2002
    ..This work provides support for the role of CD81 in the regulation of astrocyte and microglial number, perhaps by regulating cell proliferation by a contact inhibition-dependent mechanism...