Research Topics
Species | Holden T MaeckerSummaryAffiliation: BD Biosciences Country: USA Publications
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Detail Information
Publications
Functional T cell responses to tumor antigens in breast cancer patients have a distinct phenotype and cytokine signatureMargaret Inokuma
BD Biosciences, San Jose, CA 95131, USA
J Immunol 179:2627-33. 2007..Immunotherapies directed against TAAs may need to alter these T cell signatures to be effective...
Results and harmonization guidelines from two large-scale international Elispot proficiency panels conducted by the Cancer Vaccine Consortium (CVC/SVI)Sylvia Janetzki
ZellNet Consulting, Inc, Fort Lee, NJ, USA
Cancer Immunol Immunother 57:303-15. 2008..These results provide initial harmonization guidelines to optimize Elispot assay performance to the immunotherapy community. Further optimization is in process with ongoing panels...- Flow cytometry controls, instrument setup, and the determination of positivityHolden T Maecker
BD Biosciences, San Jose, CA 95131, USA
Cytometry A 69:1037-42. 2006..In this tutorial, we classify controls in various categories, describe the options within each category, and discuss the merits of each option... - Selecting fluorochrome conjugates for maximum sensitivityHolden T Maecker
BD Biosciences, San Jose, California 95131, USA
Cytometry A 62:169-73. 2004 - T cell immunity to HIV: defining parameters of protectionHolden T Maecker
BD Biosciences, Immunocytometry Systems, 2350 Qume Drive, San Jose, CA 95131, USA
Curr HIV Res 1:249-59. 2003..These parameters may include functional avidity, epitope breadth and specificity, proliferative capacity, cytokine repertoire, degree of anergy, and differentiation phenotype, as well as magnitude, of HIV-specific CD4 and CD8 T cells... - Human CD81 directly enhances Th1 and Th2 cell activation, but preferentially induces proliferation of Th2 cells upon long-term stimulationHolden T Maecker
BD Biosciences, Immunocytometry Systems, 2350 Qume Drive, San Jose, CA 95131, USA
BMC Immunol 4:1. 2003..CD81, a cell-surface protein of the tetraspanin superfamily, has been shown to costimulate T cell activation in murine T cells, and is involved in development of Th2 immune responses in mice... - Standardization of cytokine flow cytometry assaysHolden T Maecker
BD Biosciences, San Jose, USA
BMC Immunol 6:13. 2005.... - Impact of cryopreservation on tetramer, cytokine flow cytometry, and ELISPOTHolden T Maecker
BD Biosciences, San Jose, USA
BMC Immunol 6:17. 2005..In this study, we compared the performance of these assays on leukapheresed PBMC shipped overnight in medium versus cryopreserved PBMC from matched donors... - Optimal preparation of rhesus macaque blood for cytokine flow cytometric analysisLaurel E Nomura
Biological Research and Development, Immunocytometry Systems, BD Biosciences, 2350 Qume Drive, San Jose, CA 95131, USA
Cytometry A 53:28-38. 2003..Monoclonal antibodies and fluorochromes optimized for use in macaques were included in samples examined for immune responses with the use of intracellular cytokine flow cytometry (CFC)... - Analyzing T-cell responses to cytomegalovirus by cytokine flow cytometryHolden T Maecker
BD Biosciences, San Jose, California 95131, USA
Hum Immunol 65:493-9. 2004..These data will be reviewed in terms of what they suggest about the threshold of protective T-cell immunity to CMV, and other factors in addition to T-cell frequencies that could be important in protecting from CMV-associated disease... 
Precision and linearity targets for validation of an IFNgamma ELISPOT, cytokine flow cytometry, and tetramer assay using CMV peptidesHolden T Maecker
BD Biosciences, San Jose, CA, USA
BMC Immunol 9:9. 2008..Here we assess the levels of intra-assay, inter-assay, and inter-operator precision, as well as linearity, of CD8+ T cell IFNgamma-based ELISPOT and cytokine flow cytometry (CFC), as well as tetramer assays...- Performance of plate-based cytokine flow cytometry with automated data analysisMaria A Suni
BD Biosciences, 2350 Qume Drive, San Jose, CA 95131, USA
BMC Immunol 4:9. 2003..To increase the throughput of CFC assays, we have optimized methods for stimulating, staining, and acquiring whole blood or PBMC samples in 96-well or 24-well plates... 
Standardization and optimization of multiparameter intracellular cytokine stainingLaurel Nomura
BD Biosciences, 2350 Qume Dr, San Jose, California 95131, USA
Cytometry A 73:984-91. 2008..We present data from two different ongoing multi-laboratory standardization studies, one involving 15 laboratories and one involving nine. We identify issues of variability and, where possible, offer solutions...- Quality assurance of intracellular cytokine staining assays: analysis of multiple rounds of proficiency testingMaria C Jaimes
BD Biosciences, 2350 Qume Drive, San Jose, CA 95131, USA
J Immunol Methods 363:143-57. 2011.... - Dynamics of CD4 and CD8 T cell responses to cytomegalovirus in healthy human donorsHolli S Dunn
BD Biosciences, Immunocytometry Systems, San Jose, CA 95131, USA
J Infect Dis 186:15-22. 2002..By understanding the normal dynamics of CMV responses over time, it may be possible to better identify aberrant responses to CMV in immunocompromised hosts... - Cytokine flow cytometry: a multiparametric approach for assessing cellular immune responses to viral antigensVernon C Maino
BD Biosciences, San Jose, CA 95131, USA
Clin Immunol 110:222-31. 2004..These ideas are discussed in the context of new developments in sample preparation and analysis automation... - Ex vivo analysis of T-cell functionMaria A Suni
Becton, Dickinson and Company Biosciences, 2350 Qume Drive, San Jose, California 95131, USA
Curr Opin Immunol 17:434-40. 2005..Major recent developments in this area include the ability to measure T-cell degranulation via cell surface exposure of CD107 and the use of polychromatic flow cytometry to examine multiple phenotypes and functions of responding T cells... - Cytokine flow cytometryHolden T Maecker
Immunocytometry Systems, BD Biosciences, San Jose, CA, USA
Methods Mol Biol 263:95-108. 2004..In this chapter, specific methodology for such intracellular staining is elaborated, with emphasis on the effects of variables such as sample type, antigens, activation conditions, sample processing, and data acquisition and analysis... - Analysis of the frequencies and of the memory T cell phenotypes of human CD8+ T cells specific for influenza A virusesXiao Song He
Stanford University School of Medicine, Stanford, California, USA
J Infect Dis 187:1075-84. 2003..The different patterns of CD27 expression in influenza virus- and cytomegalovirus-specific CD8+ T cells suggest that influenza virus-specific memory and effector CD8+ T cells can be differentiated by phenotypic analysis... - An analytical workflow for investigating cytokine profilesJanet C Siebert
CytoAnalytics, Analytical Services, Denver, Colorado 80209, USA
Cytometry A 73:289-98. 2008..In conclusion, the analytical workflow described herein yielded statistically supported and biologically relevant findings that were otherwise unapparent... - Results of a cytomegalovirus (CMV)-specific CD8+/interferon- gamma+ cytokine flow cytometry assay correlate with clinical evidence of protective immunity in patients with AIDS with CMV retinitisMark A Jacobson
Positive Health Program, Department of Medicine, and San Francisco General Hospital General Clinical Research Center, University of California San Francisco, San Francisco, California, USA
J Infect Dis 189:1362-73. 2004..These data suggest that further testing of the CMV-specific CD8+/IFN- gamma+ CFC assay, for clinical utility in predicting incident and progressive CMVR disease, is warranted... - Poor predictive value of cytomegalovirus (CMV)-specific T cell assays for the development of CMV retinitis in patients with AIDSMark A Jacobson
Positive Health Program, Department of Medicine, University of California San Francisco, CA, USA
Clin Infect Dis 46:458-66. 2008..We examined the potential clinical utility of using a cytomegalovirus (CMV)-specific T cell immunoassay to determine the risk of developing new-onset CMV retinitis (CMVR) in patients with acquired immunodeficiency syndrome (AIDS)... - Development and dynamics of robust T-cell responses to CML under imatinib treatmentChristiane I U Chen
Department of Medicine, Division of Hematology, Stanford University School of Medicine, Stanford, CA 94305, USA
Blood 111:5342-9. 2008..This confirms the immune system's ability to respond to leukemia under certain conditions. Such responses may be further amplified as a potential therapy that synergizes with imatinib for improved control of CML... - Epigenetic changes in tumor Fas levels determine immune escape and response to therapyHeather L Maecker
Department of Radiation Oncology, Stanford University Medical Center, CCSR South, 269 Campus Drive, Stanford, California 94305, USA
Cancer Cell 2:139-48. 2002..This work demonstrates the importance of epigenetic modification of Fas in tumor progression and immune evasion, and emphasizes the essential interplay between Fas and innate immunity in the control of chemoresistant tumors... - Protective immunity to cytomegalovirus (CMV) retinitis in AIDS is associated with CMV-specific T cells that express interferon- gamma and interleukin-2 and have a CD8+ cell early maturational phenotypeElizabeth Sinclair
Division of Experimental Medicine, University of California, San Francisco, CA, USA
J Infect Dis 194:1537-46. 2006..Recovery from AIDS-related CMVR after the initiation of antiretroviral therapy may be mediated by CMV-specific CD4(+) and CD8(+) T cells capable of promoting antigen-specific CD8(+) T cell proliferation... - TNF-alpha detection using a flow cytometric assay to determine cellular responses to anthrax vaccineAnnie H Shinn
Thomas J Long School of Pharmacy, University of the Pacific, Stockton, CA 95211, USA
J Immunol Methods 282:169-74. 2003..5 microg/ml and IFN-gamma production was not detected. This assay will be used in future larger prospective studies to further evaluate the cellular immune responses induced by the anthrax vaccine... - Maximizing the retention of antigen specific lymphocyte function after cryopreservationMary L Disis
Tumor Vaccine Group, Center for Translational Medicine in Women s Health, 815 Mercer Street, 2nd Floor, Box 358050, University of Washington, Seattle, WA 98195 8050, USA
J Immunol Methods 308:13-8. 2006..I.) of the non-cryopreserved PBMC and SI obtained from cells frozen with media containing human serum albumin as compared to other additives such as dextran or fetal bovine serum... - Strong cell-mediated immune responses are associated with the maintenance of low-level viremia in antiretroviral-treated individuals with drug-resistant human immunodeficiency virus type 1Steven G Deeks
Department of Medicine, University of California, San Francisco, San Francisco, California, USA
J Infect Dis 189:312-21. 2004..Collectively, these data suggest that the selection of drug-resistance mutations may reduce the pathogenic potential of HIV, which leads to a balanced state of enhanced cellular immunity and low-level viremia... - Persistent and selective deficiency of CD4+ T cell immunity to cytomegalovirus in immunocompetent young childrenWenwei Tu
Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305, USA
J Immunol 172:3260-7. 2004..Thus, CD4(+) T cell-mediated immunity to CMV in humans is generated in an age-dependent manner, and may have a substantial role in controlling renal viral replication and urinary shedding... - Multicolor flow cytometric analysis in SIV-infected rhesus macaqueJoshua M Walker
Vaccine and Gene Therapy Institute, Oregon National Primate Research Center, Oregon Health and Science University, West Campus Beaverton, Oregon 97006, USA
Methods Cell Biol 75:535-57. 2004 - Dysfunction of simian immunodeficiency virus/simian human immunodeficiency virus-induced IL-2 expression by central memory CD4+ T lymphocytesYue Sun
Division of Viral Pathogenesis, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA
J Immunol 174:4753-60. 2005..Persisting clinical protection in vaccinated and challenged monkeys is thus correlated with a preserved capacity of the peripheral blood central memory CD4+ T cells to express this important immunomodulatory cytokine... - Immune responses in the draining lymph nodes against cancer: implications for immunotherapySuyu Shu
Center for Surgery Research, The Cleveland Clinic Foundation, Cleveland, OH, USA
Cancer Metastasis Rev 25:233-42. 2006..A clinical trial is now being carried out to test the immunogenicity and therapeutic effects of fusion hybrids for the treatment of metastatic melanoma... - Immune dysfunction and micrometastases in women with breast cancerMichael J Campbell
Department of Surgery, University of California, San Francisco, CA 94115, USA
Breast Cancer Res Treat 91:163-71. 2005..These dysregulated T cell responses did not correlate with age, stage of disease, or nodal status. However, we did observe a correlation between number of micrometastases in the bone marrow and T cell responsiveness... - Increased brain size and glial cell number in CD81-null miceEldon E Geisert
Department of Anatomy and Neurobiology, and Neuroscience Institute, University of Tennessee, Health Science Center, Memphis, Tennessee 38163, USA
J Comp Neurol 453:22-32. 2002..This work provides support for the role of CD81 in the regulation of astrocyte and microglial number, perhaps by regulating cell proliferation by a contact inhibition-dependent mechanism...