Research Topics
Genomes and Genes | Benigno ValdezSummaryAffiliation: Baylor College of Medicine Country: USA Publications
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Publications
Cloning and characterization of Gu/RH-II binding proteinB C Valdez
Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030, USA
Biochem Biophys Res Commun 234:335-40. 1997..The GBP mRNA level is highest in testis, 9- to 49-fold greater than other tissues. When GBP interacts with Gu/RH-II, proteolytic cleavage of Gu/RH-II occurs; the amino-terminal portion of Gu/RH-II is critical for this proteolysis...
Cloning and characterization of a new silver-stainable protein SSP29, a member of the LRR familyL Zhu
Department of Pharmacology, Baylor College of Medicine, Houston, TX 77030, USA
Biochem Mol Biol Int 42:927-35. 1997..Its interaction with RNA polymerase I suggests that SSP29 may participate in signal transduction that directs nucleolar activities by regulating ribosomal RNA biosynthesis...
RNA-unwinding and RNA-folding activities of RNA helicase II/Gu--two activities in separate domains of the same proteinB C Valdez
Department of Pharmacology, Baylor College of Medicine, Houston, TX 77030, USA
Eur J Biochem 250:800-7. 1997..Amino acids 646-801 fold single-stranded RNA but lack helicase activity. This report shows distinct RNA-unwinding and RNA-folding activities residing in separate domains within the same protein...
Green fluorescent protein tag for studies of drug-induced translocation of nucleolar protein RH-II/GuB C Valdez
Department of Pharmacology, Baylor College of Medicine, Houston, TX 77030, USA
Biotechniques 24:1032-6. 1998..Real-time results are obtained more quickly without the disadvantages associated with cell fixation and immunofluorescence-based staining...
Mouse RNA helicase II/Gu: cDNA and genomic sequences, chromosomal localization, and regulation of expressionB C Valdez
Department of Pharmacology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas, 77030, USA
Genomics 66:184-94. 2000..Both mouse and human RH II/Gu genes are localized on chromosome 10. The availability of the mouse RH II/Gu gene will facilitate its functional analysis including creation of a mouse deficient in RH II/Gu protein...
Structural domains involved in the RNA folding activity of RNA helicase II/Gu proteinB C Valdez
Department of Pharmacology, Baylor College of Medicine, Houston, USA
Eur J Biochem 267:6395-402. 2000..The last 17 amino acids of RH II/Gu can be replaced by an RNA binding motif from nucleolar protein p120 without a deleterious effect on its foldase activity. A model is proposed to explain how RH II/Gu binds and folds an RNA substrate...
The Treacher Collins syndrome (TCOF1) gene product is involved in ribosomal DNA gene transcription by interacting with upstream binding factorBenigno C Valdez
Department of Pharmacology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
Proc Natl Acad Sci U S A 101:10709-14. 2004..The elucidation of a physiological function of treacle provides important information of relevance to the molecular dissection of the biochemical pathology of TCS...
Expression, cellular localization, and enzymatic activities of RNA helicase II/Gu(beta)Benigno C Valdez
Department of Pharmacology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
Exp Cell Res 276:249-63. 2002..Instead, the presence of RH-II/Gu(beta) stimulates RH-II/Gu(alpha) unwinding activity. Our data suggest distinct and complex regulation of expression of the two paralogues with nonredundant gene products...
The Treacher Collins syndrome (TCOF1) gene product is involved in pre-rRNA methylationBianca Gonzales
Department of Pharmacology, Baylor College of Medicine, Houston, TX 77030, USA
Hum Mol Genet 14:2035-43. 2005....
Functional interaction between RNA helicase II/Gu(alpha) and ribosomal protein L4Hushan Yang
Department of Pharmacology, Baylor College of Medicine, Houston, TX 77030, USA
FEBS J 272:3788-802. 2005..These observations have suggested that the function of Gu(alpha) in rRNA processing is at least partially dependent on its ability to interact with RPL4...
Cloning and functional characterization of the Xenopus orthologue of the Treacher Collins syndrome (TCOF1) gene productBianca Gonzales
Department of Pharmacology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030 USA
Gene 359:73-80. 2005..laevis oocytes resulted in inhibition of rDNA gene transcription. The results suggest evolutionary conservation of the function of treacle in ribosomal RNA biogenesis in higher eukaryotes...
c-Jun supports ribosomal RNA processing and nucleolar localization of RNA helicase DDX21Tim H Holmström
Centre for Biotechnology, University of Turku and Abo Akademi University, 20520 Turku, Finland
J Biol Chem 283:7046-53. 2008..Moreover, these results suggest that the phenotypes observed previously in c-Jun-depleted mouse models and cell lines could be partly due to the effects of c-Jun on rRNA processing...
Another face of the Treacher Collins syndrome (TCOF1) gene: identification of additional exonsRolando B So
Department of Pharmacology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
Gene 328:49-57. 2004..The discovery of exons 6A and 16A is relevant to mutational analysis of the TCOF1 gene in TCS patients, and to functional analysis of its gene product...
Silencing of RNA helicase II/Gualpha inhibits mammalian ribosomal RNA productionDale Henning
Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030, USA
J Biol Chem 278:52307-14. 2003..Both down-regulation of RH-II/Gualpha and overexpression of RH-II/Gubeta slowed cell proliferation. The opposite effects of the two paralogues suggest antagonistic functions...
Down-regulation of RNA helicase II/Gu results in the depletion of 18 and 28 S rRNAs in Xenopus oocyteHushan Yang
Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030, USA
J Biol Chem 278:38847-59. 2003..Our results show that xGu is involved in the processing of 20 to 18 S rRNA and contributes to the stability of 28 S rRNA in Xenopus oocytes...
Drug-induced translocation of nucleolar proteins fused to green fluorescent proteinBenigno C Valdez
Department of Pharmacology, Baylor College of Medicine, Houston, TX, USA
Methods Mol Biol 183:151-61. 2002
Genomic structure of newly identified paralogue of RNA helicase II/Gu: detection of pseudogenes and multiple alternatively spliced mRNAsBenigno C Valdez
Department of Pharmacology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
Gene 284:53-61. 2002..No alternative splicing was identified for RH-II/Gualpha. Overall, the results suggest that the two paralogues of RH-II/Gu arose by gene duplication but the resulting genes are differentially regulated...
The DEXD/H-box RNA helicase RHII/Gu is a co-factor for c-Jun-activated transcriptionJukka Westermarck
EMBL, D 69117 Heidelberg, Germany
EMBO J 21:451-60. 2002..These findings clarify the mechanism of c-Jun-mediated transcriptional regulation, and provide evidence for an involvement of RHII/Gu in stress response and in RNA polymerase II-catalyzed transcription in mammalian cells...
Protein inhibitor of activated signal transducer and activator of transcription (STAT)-1 (PIAS-1) regulates the IFN-gamma response in macrophage cell linesEliana M Coccia
Laboratory of Immunology, Viale Regina Elena 299, 00161 Rome, Italy
Cell Signal 14:537-45. 2002..D1. Thus, our results indicate that PIAS-1 may account for the differential IFN-gamma responsiveness in macrophage cell lines at different stages of maturation...
Altered gene expression in busulfan-resistant human myeloid leukemiaBenigno C Valdez
Department of Stem Cell Transplantation and Cellular Therapy, University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, United States
Leuk Res 32:1684-97. 2008....
Research Grants
- AUTOANTIGEN RNA HELICASE II/GUBenigno Valdez; Fiscal Year: 2003..This study may also provide an impetus for understanding the pathological roles of RH-II/Gu on the elicitation of autoimmune responses. ..
