Ann M Leen

Summary

Affiliation: Baylor College of Medicine
Country: USA

Publications

  1. ncbi request reprint Fiber-modified adenoviruses generate subgroup cross-reactive, adenovirus-specific cytotoxic T lymphocytes for therapeutic applications
    Ann M Leen
    Center for Cell and Gene Therapy, Department of Pediatrics, 6621 Fannin S, MC 3 3320, Houston, TX 77030, USA
    Blood 103:1011-9. 2004
  2. ncbi request reprint Characterization of latent membrane protein 2 specificity in CTL lines from patients with EBV-positive nasopharyngeal carcinoma and lymphoma
    Karin C Straathof
    Center for Cell and Gene Therapy, Baylor College of Medicine, Methodist Hospital and Texas Children s Hospital, Houston, TX 77030, USA
    J Immunol 175:4137-47. 2005
  3. pmc Challenges of T cell therapies for virus-associated diseases after hematopoietic stem cell transplantation
    Ann M Leen
    The Methodist Hospital, Texas Children s Hospital, Center for Cell and Gene Therapy, Baylor College of Medicine, 1102 Bates Street, Houston, TX 77030, USA
    Expert Opin Biol Ther 10:337-51. 2010
  4. pmc Cytotoxic T lymphocytes as immune-therapy in haematological practice
    Ann M Leen
    Center for Cell and Gene Therapy, Baylor College of Medicine, The Methodist Hospital, and Texas Children s Hospital, Houston, TX 77030, USA
    Br J Haematol 143:169-79. 2008
  5. pmc Cytotoxic T lymphocyte therapy with donor T cells prevents and treats adenovirus and Epstein-Barr virus infections after haploidentical and matched unrelated stem cell transplantation
    Ann M Leen
    Center for Cell and Gene Therapy and Departments of Pediatrics, Immunology, Medicine, and Virology, Baylor College of Medicine, Texas Children s Hospital and The Methodist Hospital, Houston, TX 77030, USA
    Blood 114:4283-92. 2009
  6. pmc Identification of hexon-specific CD4 and CD8 T-cell epitopes for vaccine and immunotherapy
    Ann M Leen
    Center for Cell and Gene Therapy, Baylor College of Medicine, 6621 Fannin Street, MC 3 3320, Houston, TX 77030, USA
    J Virol 82:546-54. 2008
  7. pmc Cytotoxic T lymphocytes simultaneously targeting multiple tumor-associated antigens to treat EBV negative lymphoma
    Ulrike Gerdemann
    Center for Cell and Gene Therapy, Baylor College of Medicine, The Methodist Hospital, Texas Children s Hospital, Houston, Texas 77030, USA
    Mol Ther 19:2258-68. 2011
  8. pmc Production of good manufacturing practice-grade cytotoxic T lymphocytes specific for Epstein-Barr virus, cytomegalovirus and adenovirus to prevent or treat viral infections post-allogeneic hematopoietic stem cell transplant
    Uluhan Sili
    Center for Cell and Gene Therapy, Baylor College of Medicine, Texas Children s Hospital, The Methodist Hospital, Houston, Texas 77030, USA
    Cytotherapy 14:7-11. 2012
  9. pmc Genetic modification of T cells with IL-21 enhances antigen presentation and generation of central memory tumor-specific cytotoxic T-lymphocytes
    Anjum S Kaka
    Center for Cell and Gene Therapy, Baylor College of Medicine, The Methodist Hospital and Texas Children s Hospital, Houston, TX, USA
    J Immunother 32:726-36. 2009
  10. pmc Exploiting cytokine secretion to rapidly produce multivirus-specific T cells for adoptive immunotherapy
    Yuriko Fujita
    Center for Cell and Gene Therapy, Baylor College of Medicine, The Methodist Hospital, Texas Children s Hospital, Houston, TX 77030, USA
    J Immunother 31:665-74. 2008

Collaborators

Detail Information

Publications42

  1. ncbi request reprint Fiber-modified adenoviruses generate subgroup cross-reactive, adenovirus-specific cytotoxic T lymphocytes for therapeutic applications
    Ann M Leen
    Center for Cell and Gene Therapy, Department of Pediatrics, 6621 Fannin S, MC 3 3320, Houston, TX 77030, USA
    Blood 103:1011-9. 2004
    ..This is the first report of a simple and reproducible method to activate and expand Ad-specific cytotoxic T lymphocytes (CTLs), which should be protective against the range of different Ad subtypes that affect transplant recipients...
  2. ncbi request reprint Characterization of latent membrane protein 2 specificity in CTL lines from patients with EBV-positive nasopharyngeal carcinoma and lymphoma
    Karin C Straathof
    Center for Cell and Gene Therapy, Baylor College of Medicine, Methodist Hospital and Texas Children s Hospital, Houston, TX 77030, USA
    J Immunol 175:4137-47. 2005
    ..These new LMP2 epitopes broaden the diversity of HLA alleles with available epitopes, and, in particular, those epitopes conserved between EBV strains provide valuable tools for immunotherapy and immune monitoring...
  3. pmc Challenges of T cell therapies for virus-associated diseases after hematopoietic stem cell transplantation
    Ann M Leen
    The Methodist Hospital, Texas Children s Hospital, Center for Cell and Gene Therapy, Baylor College of Medicine, 1102 Bates Street, Houston, TX 77030, USA
    Expert Opin Biol Ther 10:337-51. 2010
    ..However, alternative donor HSCT are associated with three major complications i) graft rejection; ii) graft-versus-host disease (GvHD); and iii) delayed immune reconstitution leading to viral infections and relapse...
  4. pmc Cytotoxic T lymphocytes as immune-therapy in haematological practice
    Ann M Leen
    Center for Cell and Gene Therapy, Baylor College of Medicine, The Methodist Hospital, and Texas Children s Hospital, Houston, TX 77030, USA
    Br J Haematol 143:169-79. 2008
    ....
  5. pmc Cytotoxic T lymphocyte therapy with donor T cells prevents and treats adenovirus and Epstein-Barr virus infections after haploidentical and matched unrelated stem cell transplantation
    Ann M Leen
    Center for Cell and Gene Therapy and Departments of Pediatrics, Immunology, Medicine, and Virology, Baylor College of Medicine, Texas Children s Hospital and The Methodist Hospital, Houston, TX 77030, USA
    Blood 114:4283-92. 2009
    ..This trial was registered at www.clinicaltrials.gov as #NCT00590083...
  6. pmc Identification of hexon-specific CD4 and CD8 T-cell epitopes for vaccine and immunotherapy
    Ann M Leen
    Center for Cell and Gene Therapy, Baylor College of Medicine, 6621 Fannin Street, MC 3 3320, Houston, TX 77030, USA
    J Virol 82:546-54. 2008
    ....
  7. pmc Cytotoxic T lymphocytes simultaneously targeting multiple tumor-associated antigens to treat EBV negative lymphoma
    Ulrike Gerdemann
    Center for Cell and Gene Therapy, Baylor College of Medicine, The Methodist Hospital, Texas Children s Hospital, Houston, Texas 77030, USA
    Mol Ther 19:2258-68. 2011
    ..Infusion of such multispecific monocultures may extend the benefits of CTL therapy to treatment even of EBV negative HL and NHL...
  8. pmc Production of good manufacturing practice-grade cytotoxic T lymphocytes specific for Epstein-Barr virus, cytomegalovirus and adenovirus to prevent or treat viral infections post-allogeneic hematopoietic stem cell transplant
    Uluhan Sili
    Center for Cell and Gene Therapy, Baylor College of Medicine, Texas Children s Hospital, The Methodist Hospital, Houston, Texas 77030, USA
    Cytotherapy 14:7-11. 2012
    ..We now provide the details and logistics of T cell manufacture...
  9. pmc Genetic modification of T cells with IL-21 enhances antigen presentation and generation of central memory tumor-specific cytotoxic T-lymphocytes
    Anjum S Kaka
    Center for Cell and Gene Therapy, Baylor College of Medicine, The Methodist Hospital and Texas Children s Hospital, Houston, TX, USA
    J Immunother 32:726-36. 2009
    ....
  10. pmc Exploiting cytokine secretion to rapidly produce multivirus-specific T cells for adoptive immunotherapy
    Yuriko Fujita
    Center for Cell and Gene Therapy, Baylor College of Medicine, The Methodist Hospital, Texas Children s Hospital, Houston, TX 77030, USA
    J Immunother 31:665-74. 2008
    ..By simultaneously expanding a portion of the selected product, we can characterize phenotype and function of the infused product and link them with subsequent in vivo outcome...
  11. pmc Functionally active virus-specific T cells that target CMV, adenovirus, and EBV can be expanded from naive T-cell populations in cord blood and will target a range of viral epitopes
    Patrick J Hanley
    Department of Immunology, Baylor College of Medicine, Texas Children s Hospital and The Methodist Hospital, Center for Cell and Gene Therapy, Houston, TX 77030, USA
    Blood 114:1958-67. 2009
    ..This study is registered at www.clinicaltrials.gov as NCT00078533...
  12. pmc Using dendritic cell maturation and IL-12 producing capacity as markers of function: a cautionary tale
    Anjum S Kaka
    Center for Cell and Gene Therapy, Baylor College of Medicine, The Methodist Hospital and Texas Children s Hospital, Houston, TX 77030, USA
    J Immunother 31:359-69. 2008
    ....
  13. pmc Complete responses of relapsed lymphoma following genetic modification of tumor-antigen presenting cells and T-lymphocyte transfer
    Catherine M Bollard
    Center for Cell and Gene Therapy, Baylor College of Medicine, The Methodist Hospital and Texas Children s Hospital, Houston, TX 77030, USA
    Blood 110:2838-45. 2007
    ..This study is registered at http://www.cancer.gov/clinicaltrials (protocol IDs: BCM-H-9936, NCT00062868, NCT00070226)...
  14. pmc Derivation of human T lymphocytes from cord blood and peripheral blood with antiviral and antileukemic specificity from a single culture as protection against infection and relapse after stem cell transplantation
    Kenneth P Micklethwaite
    Center for Cell and Gene Therapy, Baylor College of Medicine, Texas Children s Hospital, Houston, TX, USA
    Blood 115:2695-703. 2010
    ..This approach may prove beneficial for patients with high-risk B-ALL who have recently received an HSC or CB transplant and are at risk of infection and relapse...
  15. pmc Accelerated production of antigen-specific T cells for preclinical and clinical applications using gas-permeable rapid expansion cultureware (G-Rex)
    Juan F Vera
    Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX 77030, USA
    J Immunother 33:305-15. 2010
    ..This bioprocess optimization increased T-cell output while decreasing the complexity and cost of CTL manufacture, making cell therapy more accessible...
  16. pmc Antigen-specific cytotoxic T lymphocytes can target chemoresistant side-population tumor cells in Hodgkin lymphoma
    Jessica A Shafer
    Center for Cell and Gene Therapy, Department of Pediatrics, Baylor College of Medicine, The Methodist Hospital and Texas Children s Cancer Center, Houston, TX 77030, USA
    Leuk Lymphoma 51:870-80. 2010
    ..This study suggests that chemoresistant HL SP cells can be targeted by the immune system, providing a rationale for combined chemotherapy and immunotherapy for the treatment of HL...
  17. pmc Immunotherapeutic strategies to prevent and treat human herpesvirus 6 reactivation after allogeneic stem cell transplantation
    Ulrike Gerdemann
    Center for Cell and Gene Therapy, Baylor College of Medicine, Methodist Hospital, Texas Children s Hospital, Houston, TX 77030, USA
    Blood 121:207-18. 2013
    ..Thus, we conclude that adoptive T-cell immunotherapy for HHV6 is a practical objective and that the peptide and epitope tools we describe will allow such cells to be prepared, administered, and monitored in human subjects...
  18. pmc Nucleofection of DCs to generate Multivirus-specific T cells for prevention or treatment of viral infections in the immunocompromised host
    Ulrike Gerdemann
    Center for Cell and Gene Therapy, Baylor College of Medicine, The Methodist Hospital, Texas Children s Hospital, Houston, Texas 77030, USA
    Mol Ther 17:1616-25. 2009
    ..These CTL generation procedures should increase the feasibility and applicability of T-cell therapy...
  19. ncbi request reprint Conserved CTL epitopes on the adenovirus hexon protein expand subgroup cross-reactive and subgroup-specific CD8+ T cells
    Ann M Leen
    Center for Cell and Gene Therapy, Department of Pediatrics, 6621 Fannin St, MC 3 3320, Houston, TX, USA
    Blood 104:2432-40. 2004
    ..Thus, by exploiting these peptide targets, it is possible to prepare a T-cell population capable of reacting with most adenoviruses that cause disease in immunocompromised patients...
  20. ncbi request reprint Monoculture-derived T lymphocytes specific for multiple viruses expand and produce clinically relevant effects in immunocompromised individuals
    Ann M Leen
    Center for Cell and Gene Therapy, Baylor College of Medicine, The Methodist Hospital and Texas Children s Hospital, Houston, Texas 77030, USA
    Nat Med 12:1160-6. 2006
    ..Monoculture-derived multispecific CTL infusion could provide a safe and efficient means to restore virus-specific immunity in the immunocompromised host...
  21. pmc Rapidly generated multivirus-specific cytotoxic T lymphocytes for the prophylaxis and treatment of viral infections
    Ulrike Gerdemann
    Center for Cell and Gene Therapy, Baylor College of Medicine, The Methodist Hospital, Texas Children s Hospital, Houston, TX, USA
    Mol Ther 20:1622-32. 2012
    ..Our approach can be readily introduced into clinical practice and should be a cost-effective alternative to common antiviral prophylactic agents for allogeneic hematopoietic stem cell transplant (HSCT) recipients...
  22. pmc Improving T-cell therapy for relapsed EBV-negative Hodgkin lymphoma by targeting upregulated MAGE-A4
    Conrad R Cruz
    Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, Texas
    Clin Cancer Res 17:7058-66. 2011
    ..Furthermore, epigenetic modification by clinically available demethylating agents can enhance MAGE-A4 expression in previously MAGE-negative tumors...
  23. pmc Genetic manipulation of tumor-specific cytotoxic T lymphocytes to restore responsiveness to IL-7
    Juan F Vera
    Center for Cell and Gene Therapy, Baylor College of Medicine, The Methodist Hospital, Houston, Texas 77030, USA
    Mol Ther 17:880-8. 2009
    ..This approach may allow selective expansion of CTLs without the unwanted effects associated with IL-2...
  24. ncbi request reprint Autologous designer antigen-presenting cells by gene modification of T lymphocyte blasts with IL-7 and IL-12
    Aaron E Foster
    Center for Cell and Gene Therapy, Baylor College of Medicine, The Methodist Hospital and Texas Children s Hospital, 6621 Fannin Street, Houston, TX 77030, USA
    J Immunother 30:506-16. 2007
    ..Thus, T cells provide a platform for the generation of autologous APC that can be customized to express both antigens and therapeutic molecules for the induction of antigen-specific T cell immunity...
  25. ncbi request reprint Adenoviral infections in hematopoietic stem cell transplantation
    Ann M Leen
    Center for Cell and Gene Therapy, Baylor College of Medicine, and The Methodist Hospital, Houston, Texas 77030, USA
    Biol Blood Marrow Transplant 12:243-51. 2006
    ..We discuss the advantages and limitations of T-cell therapy for the prophylaxis and treatment of adenovirus infection posttransplantation...
  26. pmc Multicenter study of banked third-party virus-specific T cells to treat severe viral infections after hematopoietic stem cell transplantation
    Ann M Leen
    Center for Cell and Gene Therapy, Baylor College of Medicine, Texas Children s Hospital, The Methodist Hospital, Houston, TX 77030, USA
    Blood 121:5113-23. 2013
    ..The use of banked third-party VSTs is a feasible and safe approach to rapidly treat severe or intractable viral infections after stem cell transplantation. This study is registered at www.clinicaltrials.gov as NCT00711035. ..
  27. pmc PiggyBac-mediated cancer immunotherapy using EBV-specific cytotoxic T-cells expressing HER2-specific chimeric antigen receptor
    Yozo Nakazawa
    Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, Texas 77030, USA
    Mol Ther 19:2133-43. 2011
    ..Our results demonstrate that PB can be used to redirect virus-specific CTLs to tumor targets, which should prolong tumor-specific T cell survival in vivo producing more efficacious immunotherapy...
  28. pmc Safety and clinical efficacy of rapidly-generated trivirus-directed T cells as treatment for adenovirus, EBV, and CMV infections after allogeneic hematopoietic stem cell transplant
    Ulrike Gerdemann
    Center for Cell and Gene Therapy, Baylor College of Medicine, The Methodist Hospital, Texas Children s Hospital, Houston, Texas, USA
    Mol Ther 21:2113-21. 2013
    ..This approach should increase both the feasibility and applicability of T cell therapy. The trial was registered at www.clinicaltrials.gov as NCT01070797...
  29. pmc High-avidity cytotoxic T lymphocytes specific for a new PRAME-derived peptide can target leukemic and leukemic-precursor cells
    Concetta Quintarelli
    Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX 77030, USA
    Blood 117:3353-62. 2011
    ..These PRAME-directed CTLs did not affect normal hematopoietic progenitors, indicating that this approach may be of value for immunotherapy of PRAME(+) hematologic malignancies...
  30. pmc Human papillomavirus type 16 E6/E7-specific cytotoxic T lymphocytes for adoptive immunotherapy of HPV-associated malignancies
    Carlos A Ramos
    Center for Cell and Gene Therapy, Baylor College of Medicine, The Methodist Hospital and Texas Children s Hospital, Houston, TX77030, USA
    J Immunother 36:66-76. 2013
    ..Because our technique is scalable and good-manufacturing procedures-compliant, these lines could be used for adoptive cellular immunotherapy of patients with HPV16 cancers...
  31. pmc Generation of polyclonal CMV-specific T cells for the adoptive immunotherapy of glioblastoma
    Alexia Ghazi
    Center for Cell and Gene Therapy, Baylor College of Medicine, The Methodist Hospital and Texas Children s Hospital, Houston, TX 77030, USA
    J Immunother 35:159-68. 2012
    ..Infusion of such CMV-specific T-cell lines may extend the benefits of T-cell therapy to patients with CMV GBMs...
  32. ncbi request reprint Adenovirus as an emerging pathogen in immunocompromised patients
    Ann M Leen
    Center for Cell and Gene Therapy, Baylor College of Medicine, The Methodist Hospital, and Texas Children s Hospital, Houston, TX 77030, USA
    Br J Haematol 128:135-44. 2005
    ....
  33. pmc Engineered T cells for pancreatic cancer treatment
    Usha L Katari
    Center for Cell and Gene Therapy, Baylor College of Medicine, Texas Children s Hospital, Houston, TX 77030, USA
    HPB (Oxford) 13:643-50. 2011
    ..The aim of this study is to develop an adoptive T cell transfer approach to target tumours expressing prostate stem cell antigen (PSCA), a tumour-associated antigen that is frequently expressed by pancreatic cancer cells...
  34. doi request reprint Complementation of Antigen-presenting Cells to Generate T Lymphocytes With Broad Target Specificity
    Minhtran Charlotte Ngo
    Center for Cell and Gene Therapy, Texas Children s Hospital, The Methodist Hospital, Baylor College of Medicine, Houston, TX Interdepartmental Program in Translational Biology and Medicine Medical Scientist Training Program Departments of Pediatrics Medicine Molecular Virology and Microbiology Pathology and Immunology, Baylor College of Medicine, Houston, TX
    J Immunother 37:193-203. 2014
    ..Hence, KATpx provides a robust, reliable, and scalable process to expand tumor-directed T cells for the treatment of virus-associated cancers. ..
  35. doi request reprint Is cancer gene therapy an empty suit?
    Malcolm K Brenner
    Center for Cell and Gene Therapy, Baylor College of Medicine, The Methodist Hospital and Texas Children s Hospital, Houston, TX, USA Department of Pediatrics, Baylor College of Medicine, Houston, TX, USA Department of Medicine, Baylor College of Medicine, Houston, TX, USA Electronic address
    Lancet Oncol 14:e447-56. 2013
    ..Recent and striking successes and correspondingly increased commercial involvement suggest that gene transfer could finally become a powerful method for development of safe and effective cancer therapeutic drugs. ..
  36. ncbi request reprint Improving T cell therapy for cancer
    Ann M Leen
    Center for Cell and Gene Therapy, Department of Pediatrics, Baylor College of Medicine, The Methodist Hospital and Texas Children s Hospital, Houston, Texas 77030, USA
    Annu Rev Immunol 25:243-65. 2007
    ..In this review, we discuss current, promising strategies to improve adoptive T cell therapy for the treatment of cancer...
  37. pmc Adverse events following infusion of T cells for adoptive immunotherapy: a 10-year experience
    Conrad Russell Cruz
    Dan L Duncan Cancer Center for Cell and Gene Therapy, Baylor College of Medicine, The Methodist Hospital, Texas Children s Hospital, Houston, Texas 77030, USA
    Cytotherapy 12:743-9. 2010
    ..Recent catastrophic reactions to 'first-in-man' biologic agents have emphasized the importance of this rule for initial studies of new products. The value of such monitoring for better established agents is less obvious...
  38. doi request reprint Kinetics of tumor destruction by chimeric antigen receptor-modified T cells
    Usanarat Anurathapan
    Center for Cell and Gene Therapy, Baylor College of Medicine, Texas Children s Hospital, and Houston Methodist Hospital, Houston, Texas, USA
    Mol Ther 22:623-33. 2014
    ....
  39. pmc T lymphocytes targeting native receptors
    Cliona M Rooney
    Center for Cell and Gene Therapy, Baylor College of Medicine, Houston Methodist Hospital and Texas Children s Hospital, Houston, TX, USA
    Immunol Rev 257:39-55. 2014
    ....
  40. doi request reprint Antiviral T-cell therapy
    Ann M Leen
    Center for Cell and Gene Therapy, Baylor College of Medicine, Houston Methodist Hospital and Texas Children s Hospital, Houston, TX, USA
    Immunol Rev 258:12-29. 2014
    ....
  41. pmc Generation of tumor antigen-specific T cell lines from pediatric patients with acute lymphoblastic leukemia--implications for immunotherapy
    Gerrit Weber
    Authors Affiliations Center for Cell and Gene Therapy, Departments of Pediatrics, Medicine, and Immunology, Baylor College of Medicine The Methodist Hospital Texas Children s Hospital Texas Children s Cancer Center, Houston, Texas and Hematology Branch, National Heart, Lung, and Blood Institute, NIH, Bethesda, Maryland
    Clin Cancer Res 19:5079-91. 2013
    ..We explored the feasibility of generating tumor antigen-specific T cells ex vivo from the peripheral blood of 50 patients with ALL [26 National Cancer Institute (NCI) high-risk and 24 standard-risk] receiving maintenance therapy...
  42. doi request reprint T-cell therapy for viral infections
    Helen E Heslop
    1Center for Cell and Gene Therapy, Baylor College of Medicine, Texas Children s Hospital, The Methodist Hospital, Houston, TX
    Hematology Am Soc Hematol Educ Program 2013:342-7. 2013
    ..The goal of current trials is to make this approach more broadly applicable using more rapidly available products from the donor, such as directly selected or briefly expanded cells or closely matched banked cells. ..