P J Hastings

Summary

Affiliation: Baylor College of Medicine
Country: USA

Publications

  1. pmc R-loops and nicks initiate DNA breakage and genome instability in non-growing Escherichia coli
    Hallie Wimberly
    Department of Molecular and Human Genetics, 1 Baylor Plaza, Houston, Texas 77030, USA
    Nat Commun 4:2115. 2013
  2. pmc Global chromosomal structural instability in a subpopulation of starving Escherichia coli cells
    Dongxu Lin
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, United States of America
    PLoS Genet 7:e1002223. 2011
  3. pmc Mechanisms of change in gene copy number
    P J Hastings
    Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    Nat Rev Genet 10:551-64. 2009
  4. pmc Competition of Escherichia coli DNA polymerases I, II and III with DNA Pol IV in stressed cells
    P J Hastings
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, United States of America
    PLoS ONE 5:e10862. 2010
  5. ncbi request reprint Adaptive amplification
    P J Hastings
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Crit Rev Biochem Mol Biol 42:271-83. 2007
  6. ncbi request reprint SOS mutator DNA polymerase IV functions in adaptive mutation and not adaptive amplification
    G J McKenzie
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030 3411, USA
    Mol Cell 7:571-9. 2001
  7. pmc Replicative mechanisms for CNV formation are error prone
    Claudia M B Carvalho
    1 Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA 2 Centro de Pesquisas René Rachou FIOCRUZ, Belo Horizonte, Brazil
    Nat Genet 45:1319-26. 2013
  8. ncbi request reprint Adaptive mutation and amplification in Escherichia coli: two pathways of genome adaptation under stress
    Megan N Hersh
    Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Rm S809, Mail Stop 225, Houston, TX 77030 3411, USA
    Res Microbiol 155:352-9. 2004
  9. pmc Separate DNA Pol II- and Pol IV-dependent pathways of stress-induced mutation during double-strand-break repair in Escherichia coli are controlled by RpoS
    Ryan L Frisch
    Department of Molecular and Human Genetics, Baylor College of Medicine, Baylor College of Medicine, Houston, Texas 77030 3411, USA
    J Bacteriol 192:4694-700. 2010
  10. pmc Adaptive amplification and point mutation are independent mechanisms: evidence for various stress-inducible mutation mechanisms
    P J Hastings
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA
    PLoS Biol 2:e399. 2004

Collaborators

Detail Information

Publications33

  1. pmc R-loops and nicks initiate DNA breakage and genome instability in non-growing Escherichia coli
    Hallie Wimberly
    Department of Molecular and Human Genetics, 1 Baylor Plaza, Houston, Texas 77030, USA
    Nat Commun 4:2115. 2013
    ....
  2. pmc Global chromosomal structural instability in a subpopulation of starving Escherichia coli cells
    Dongxu Lin
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, United States of America
    PLoS Genet 7:e1002223. 2011
    ..These data imply that chromosomal structural changes occur in bursts or showers of instability that may have the potential to drive rapid evolution...
  3. pmc Mechanisms of change in gene copy number
    P J Hastings
    Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    Nat Rev Genet 10:551-64. 2009
    ..For example, cellular stress might induce repair of broken replication forks to switch from high-fidelity homologous recombination to non-homologous repair, thus promoting copy number change...
  4. pmc Competition of Escherichia coli DNA polymerases I, II and III with DNA Pol IV in stressed cells
    P J Hastings
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, United States of America
    PLoS ONE 5:e10862. 2010
    ..This mutagenesis assay reveals the DNA polymerases operating in DSB repair during stress and also provides a sensitive indicator for DNA polymerase competition and choice in vivo...
  5. ncbi request reprint Adaptive amplification
    P J Hastings
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    Crit Rev Biochem Mol Biol 42:271-83. 2007
    ..Recent advances in our understanding of amplification in bacteria suggests new interpretations of events leading to human copy number variation, as well as evolution in general...
  6. ncbi request reprint SOS mutator DNA polymerase IV functions in adaptive mutation and not adaptive amplification
    G J McKenzie
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030 3411, USA
    Mol Cell 7:571-9. 2001
    ..We show that pol IV, together with the major replicase, pol III, can account for all adaptive point mutations at lac. The results identify a role for pol IV in inducible genetic change...
  7. pmc Replicative mechanisms for CNV formation are error prone
    Claudia M B Carvalho
    1 Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA 2 Centro de Pesquisas René Rachou FIOCRUZ, Belo Horizonte, Brazil
    Nat Genet 45:1319-26. 2013
    ..Our findings implicate low-fidelity, error-prone DNA polymerase activity in synthesis associated with DNA repair mechanisms as the cause of local increase in point mutation burden associated with human CGR...
  8. ncbi request reprint Adaptive mutation and amplification in Escherichia coli: two pathways of genome adaptation under stress
    Megan N Hersh
    Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Rm S809, Mail Stop 225, Houston, TX 77030 3411, USA
    Res Microbiol 155:352-9. 2004
    ..Stress-induced genetic changes may underlie much of microbial evolution, pathogenesis and antibiotic resistance, and also cancer formation, progression and drug resistance...
  9. pmc Separate DNA Pol II- and Pol IV-dependent pathways of stress-induced mutation during double-strand-break repair in Escherichia coli are controlled by RpoS
    Ryan L Frisch
    Department of Molecular and Human Genetics, Baylor College of Medicine, Baylor College of Medicine, Houston, Texas 77030 3411, USA
    J Bacteriol 192:4694-700. 2010
    ..The results identify a second DNA polymerase contributing to stress-induced mutagenesis and show that RpoS promotes mutagenesis by more than the simple upregulation of dinB...
  10. pmc Adaptive amplification and point mutation are independent mechanisms: evidence for various stress-inducible mutation mechanisms
    P J Hastings
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA
    PLoS Biol 2:e399. 2004
    ..We suggest that the availability of alternative pathways for genetic/evolutionary adaptation and clonal expansion under stress may be exploited during processes ranging from the evolution of drug resistance to cancer progression...
  11. pmc Identity and function of a large gene network underlying mutagenic repair of DNA breaks
    Abu Amar M Al Mamun
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030 3411, USA
    Science 338:1344-8. 2012
    ....
  12. pmc On the mechanism of gene amplification induced under stress in Escherichia coli
    Andrew Slack
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA
    PLoS Genet 2:e48. 2006
    ..We outline its possible applicability to amplification in humans and other organisms and circumstances...
  13. pmc DinB upregulation is the sole role of the SOS response in stress-induced mutagenesis in Escherichia coli
    Rodrigo S Galhardo
    Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030 3411, USA
    Genetics 182:55-68. 2009
    ....
  14. ncbi request reprint Microbiology and evolution. Modulating mutation rates in the wild
    Susan M Rosenberg
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
    Science 300:1382-3. 2003
  15. pmc Role of RecA and the SOS response in thymineless death in Escherichia coli
    Natalie C Fonville
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA
    PLoS Genet 6:e1000865. 2010
    ..The data predict the importance of DNA damage-response and HR networks to TLD and chemotherapy resistance in humans...
  16. pmc Gross chromosomal rearrangement mediated by DNA replication in stressed cells: evidence from Escherichia coli
    J M Moore
    Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas, USA
    Ann N Y Acad Sci 1267:103-9. 2012
    ..Strains deleted for the genes for three proteins that inhibit RecA activity, psiB, dinI, and recX, all show unaltered amplification, suggesting that if they do downregulate RecA indirectly, this activity does not promote amplification...
  17. pmc Mechanisms for recurrent and complex human genomic rearrangements
    Pengfei Liu
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
    Curr Opin Genet Dev 22:211-20. 2012
    ..Both nonhomologous end-joining and aberrant replication have significant roles in chromothripsis. As we study CNV, the processes underlying human genome evolution are revealed...
  18. ncbi request reprint In pursuit of a molecular mechanism for adaptive gene amplification
    P J Hastings
    Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Room T809 Mail Stop 225, Houston, TX 77030 3411, USA
    DNA Repair (Amst) 1:111-23. 2002
    ..We explore possible molecular mechanisms of adaptive amplification in the bacterial system and note parallels to chromosomal instability in other systems...
  19. pmc Pathways of resistance to thymineless death in Escherichia coli and the function of UvrD
    Natalie C Fonville
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030 3411, USA
    Genetics 189:23-36. 2011
    ..These results define pathways by which cells resist TLD and suggest strategies for combating TLD resistance during chemotherapies...
  20. ncbi request reprint A role for topoisomerase III in a recombination pathway alternative to RuvABC
    Christopher R Lopez
    Interdepartmental Program in Cell and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Mol Microbiol 58:80-101. 2005
    ..These data are consistent with a role for topoisomerase III in disentangling recombination intermediates as an alternative to RuvABC to maintain the stability of the genome...
  21. pmc The sigma(E) stress response is required for stress-induced mutation and amplification in Escherichia coli
    Janet L Gibson
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030 3411, USA
    Mol Microbiol 77:415-30. 2010
    ..This provides the first report of mutagenesis promoted by sigma(E), and implies that extracytoplasmic stressors may affect genome integrity and, potentially, the ability to evolve...
  22. pmc Stress-induced mutation via DNA breaks in Escherichia coli: a molecular mechanism with implications for evolution and medicine
    Susan M Rosenberg
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA
    Bioessays 34:885-92. 2012
    ..New data also suggest a possible harmony between previous, apparently opposed, models for the molecular mechanism. They additionally strengthen the case for anti-evolvability therapeutics for infectious disease and cancer...
  23. pmc Mutation as a stress response and the regulation of evolvability
    Rodrigo S Galhardo
    Department of Molecular and Human Genetics, Baylor College, Houston, Texas 77030 3411, USA
    Crit Rev Biochem Mol Biol 42:399-435. 2007
    ..The emerging commonalities in stress-induced-mutation mechanisms provide hope for new therapeutic interventions for all of these processes...
  24. pmc Frequency of nonallelic homologous recombination is correlated with length of homology: evidence that ectopic synapsis precedes ectopic crossing-over
    Pengfei Liu
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
    Am J Hum Genet 89:580-8. 2011
    ..To explain this, we propose that the probability of ectopic chromosome synapsis increases with increased LCR length, and that ectopic synapsis is a necessary precursor to ectopic crossing-over...
  25. pmc Adaptive point mutation and adaptive amplification pathways in the Escherichia coli Lac system: stress responses producing genetic change
    Susan M Rosenberg
    Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, BCM S809A Mail Stop BCM225, Houston, TX 77030 3411, USA
    J Bacteriol 186:4838-43. 2004
  26. pmc A microhomology-mediated break-induced replication model for the origin of human copy number variation
    P J Hastings
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, United States of America
    PLoS Genet 5:e1000327. 2009
    ....
  27. pmc Mutability and importance of a hypermutable cell subpopulation that produces stress-induced mutants in Escherichia coli
    Caleb Gonzalez
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, United States of America
    PLoS Genet 4:e1000208. 2008
    ..e., it is important. We consider a model in which HMS differentiation is controlled by stress responses. Differentiation of an HMS potentially limits the risks of mutagenesis in cell clones...
  28. pmc Rebuttal: growth under selection stimulates Lac(+) reversion (Roth and Andersson)
    Susan M Rosenberg
    Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, BCM S809A, Mail Stop BCM225, Houston, TX 77030 3411, USA
    J Bacteriol 186:4862-3. 2004
  29. pmc Rebuttal: adaptive mutation in Escherichia coli (Foster)
    Susan M Rosenberg
    Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030 3411, USA
    J Bacteriol 186:4853. 2004
  30. ncbi request reprint Antibiotic-induced lateral transfer of antibiotic resistance
    P J Hastings
    Department of Molecular and Human Genetics, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX 77030 3411, USA
    Trends Microbiol 12:401-4. 2004
    ..Several commonly used antibiotics induce the SOS response, potentially hastening genetic change and the evolution to resistance of pathogenic populations. The use of such antibiotics should be reconsidered...
  31. pmc Chromosome catastrophes involve replication mechanisms generating complex genomic rearrangements
    Pengfei Liu
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
    Cell 146:889-903. 2011
    ..The resemblance between CGR and chromothripsis suggests similar mechanistic underpinnings. Such chromosome catastrophic events appear to reflect basic DNA metabolism operative throughout an organism's life cycle...
  32. pmc Inverted genomic segments and complex triplication rearrangements are mediated by inverted repeats in the human genome
    Claudia M B Carvalho
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA
    Nat Genet 43:1074-81. 2011
    ..We propose a mechanism that involves both homology-driven events, via inverted repeats, and microhomologous or nonhomologous events...