Research Topics
| Peter ChristieSummaryAffiliation: Baylor College of Medicine Country: USA Publications
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Detail Information
Publications
Bacterial type IV secretion: conjugation systems adapted to deliver effector molecules to host cellsP J Christie
Dept of Microbiology and Molecular Genetics, The University of Texas Houston Medical School, 6431 Fannin, Houston, TX 77030, USA
Trends Microbiol 8:354-60. 2000..Other pathogens, including Bordetella pertussis, Legionella pneumophila, Brucellaspp. and Helicobacter pylori, use type IV machines to export effector proteins to the extracellular milieu or the mammalian cell cytosol...- Type IV secretion: intercellular transfer of macromolecules by systems ancestrally related to conjugation machinesP J Christie
Department of Microbiology and Molecular Genetics, The University of Texas Houston Medical School, 6431 Fannin, Houston, TX 77030, USA
Mol Microbiol 40:294-305. 2001..The list of putative type IV family members is increasing rapidly, suggesting that macromolecular transfer by these systems is a widespread phenomenon in nature... - Biogenesis, architecture, and function of bacterial type IV secretion systemsPeter J Christie
Department of Microbiology and Molecular Genetics, UT Houston Medical School, Houston, Texas 77030, USA
Annu Rev Microbiol 59:451-85. 2005..We also summarize salient features of the increasingly studied effector translocator systems of mammalian pathogens... - Structural and dynamic properties of bacterial type IV secretion systems (review)Peter J Christie
Department of Microbiology and Molecular Genetics, UT Houston Medical School, Houston, Texas 77030, USA
Mol Membr Biol 22:51-61. 2005..Together, these recent findings describe the mechanics of type IV secretion in unprecedented detail... - Type IV secretion: the Agrobacterium VirB/D4 and related conjugation systemsPeter J Christie
Department of Microbiology and Molecular Genetics, UT Houston Medical School, 6431 Fannin, JFB1 765, Houston TX 77030, USA
Biochim Biophys Acta 1694:219-34. 2004..This review summarizes recent progress in our understanding of the mechanistic details of DNA trafficking with a focus on the paradigmatic Agrobacterium tumefaciens VirB/D4 T4SS and related conjugation systems... - Agrobacterium VirB10 domain requirements for type IV secretion and T pilus biogenesisSimon J Jakubowski
University of Texas Houston Medical School, Department of Microbiology and Molecular Genetics, 6431 Fannin Street, Houston, TX 77030, USA
Mol Microbiol 71:779-94. 2009..The data further support a model that distinct domains of VirB10 regulate formation of the secretion channel or the T pilus... - Agrobacterium VirB10, an ATP energy sensor required for type IV secretionEric Cascales
Department of Microbiology and Molecular Genetics, University of Texas-Houston Medical School, 6431 Fannin, Houston, TX 77030, USA
Proc Natl Acad Sci U S A 101:17228-33. 2004..tumefaciens cell envelope... - Energetic components VirD4, VirB11 and VirB4 mediate early DNA transfer reactions required for bacterial type IV secretionKrishnamohan Atmakuri
Department of Microbiology and Molecular Genetics, University of Texas Medical School at Houston, 6431 Fannin, JFB1 765, Houston, TX 77030, USA
Mol Microbiol 54:1199-211. 2004..Our findings define genetic requirements for DNA substrate binding and the early transfer reactions of a bacterial type IV translocation pathway... - Definition of a bacterial type IV secretion pathway for a DNA substrateEric Cascales
Department of Microbiology and Molecular Genetics, University of Texas Medical School at Houston, 6431 Fannin, JFB1 765, Houston, TX 77030, USA
Science 304:1170-3. 2004..Our results define the translocation pathway for a DNA substrate through a bacterial conjugation machine, specifying the contributions of each subunit of the secretory apparatus to substrate passage... - VirE2, a type IV secretion substrate, interacts with the VirD4 transfer protein at cell poles of Agrobacterium tumefaciensKrishnamohan Atmakuri
Department of Microbiology and Molecular Genetics, The University of Texas Houston Medical School, 6431 Fannin, Houston, TX 77030, USA
Mol Microbiol 49:1699-713. 2003..Together, our findings establish for the first time that a protein substrate of a type IV secretion system is recruited to a member of the coupling protein superfamily... - Enterococcus faecalis PcfC, a spatially localized substrate receptor for type IV secretion of the pCF10 transfer intermediateYuqing Chen
Department of Microbiology and Molecular Genetics, University of Texas Medical School at Houston, 6431 Fannin, Houston, TX 77030, USA
J Bacteriol 190:3632-45. 2008.... - Agrobacterium tumefaciens VirB6 protein participates in formation of VirB7 and VirB9 complexes required for type IV secretionSimon J Jakubowski
Department of Microbiology and Molecular Genetics, The University of Texas-Houston Medical School, Houston, Texas 77030, USA
J Bacteriol 185:2867-78. 2003..Our findings support a model that VirB6 mediates formation of VirB7 and VirB9 complexes required for biogenesis of the T pilus and the secretion channel... - Agrobacterium ParA/MinD-like VirC1 spatially coordinates early conjugative DNA transfer reactionsKrishnamohan Atmakuri
Department of Microbiology and Molecular Genetics, University of Texas Medical School at Houston, Houston, TX 77030, USA
EMBO J 26:2540-51. 2007.... - A novel cytology-based, two-hybrid screen for bacteria applied to protein-protein interaction studies of a type IV secretion systemZhiyong Ding
Department of Microbiology and Molecular Genetics, The University of Texas--Houston Medical School, Houston, Texas 77030, USA
J Bacteriol 184:5572-82. 2002..Together, our findings establish a proof-of-concept for the use of cell-location-specific proteins for studies of interactions among cytosolic and membrane proteins in diverse bacterial species... - Agrobacterium tumefaciens twin-arginine-dependent translocation is important for virulence, flagellation, and chemotaxis but not type IV secretionZhiyong Ding
Department of Microbiology and Molecular Genetics, The University of Texas-Houston Medical School, 6431 Fannin, Houston, TX 77030, USA
J Bacteriol 185:760-71. 2003..Furthermore, this secretion pathway contributes to flagellar biogenesis and chemotactic responses but not to sensory perception of plant signals or the assembly of a type IV secretion system... - Agrobacterium tumefaciens VirB9, an outer-membrane-associated component of a type IV secretion system, regulates substrate selection and T-pilus biogenesisSimon J Jakubowski
Department of Microbiology and Molecular Genetics, University of Texas Medical School at Houston, 6431 Fannin, Houston, TX 77030, USA
J Bacteriol 187:3486-95. 2005..Our findings, together with computer-based analyses, support a model in which distinct domains of VirB9 contribute to substrate selection and translocation, establishment of channel subunit contacts, and T-pilus biogenesis... - The outs and ins of bacterial type IV secretion substratesZhiyong Ding
Department of Microbiology and Molecular Genetics, The University of Texas-Houston Medical School, Houston, TX 77030, USA
Trends Microbiol 11:527-35. 2003..Rapid progress has been made toward identification of type IV secretion substrates and the requirements for substrate recognition... - The versatile bacterial type IV secretion systemsEric Cascales
Department of Microbiology and Molecular Genetics, University of Texas-Houston Medical School, Houston, Texas 77030, USA
Nat Rev Microbiol 1:137-49. 2003.... - Agrobacterium tumefaciens oncogenic suppressors inhibit T-DNA and VirE2 protein substrate binding to the VirD4 coupling proteinEric Cascales
Department of Microbiology and Molecular Genetics, University of Texas Houston, Medical School, Houston, TX 77030, USA
Mol Microbiol 58:565-79. 2005..Our findings establish that a competing plasmid substrate and a plasmid fertility inhibition factor act on a common target, the T4S receptor, to inhibit docking of DNA and protein substrates to the translocation apparatus... - Biological diversity of prokaryotic type IV secretion systemsCristina E Alvarez-Martinez
Department of Microbiology and Molecular Genetics, University of Texas Medical School at Houston, 6431 Fannin, Houston, TX 77030, USA
Microbiol Mol Biol Rev 73:775-808. 2009.... - Agrobacterium tumefaciens VirB6 domains direct the ordered export of a DNA substrate through a type IV secretion SystemSimon J Jakubowski
Department of Microbiology and Molecular Genetics, University of Texas Medical School at Houston, 77030, USA
J Mol Biol 341:961-77. 2004..Our findings support a model whereby the periplasmic loop P2 comprises a portion of the secretion channel and distinct domains of VirB6 participate in channel subunit interactions required for substrate passage to the cell exterior... - Evidence for VirB4-mediated dislocation of membrane-integrated VirB2 pilin during biogenesis of the Agrobacterium VirB/VirD4 type IV secretion systemJennifer E Kerr
Department of Microbiology and Molecular Genetics, University of Texas Medical School at Houston, 6431 Fannin St, Houston, TX 77030, USA
J Bacteriol 192:4923-34. 2010..The VirB11 ATPase functioned together with VirB4 to induce a structural change in the pilin that was detectable by MPB labeling, suggestive of a role for VirB11 as a modulator of VirB4 dislocase activity... - NMR structure of a complex between the VirB9/VirB7 interaction domains of the pKM101 type IV secretion systemRichard Bayliss
Institute of Structural Molecular Biology, University College London Birkbeck, Malet Street, London WC1E 7HX, United Kingdom
Proc Natl Acad Sci U S A 104:1673-8. 2007..tumefaciens inserts in the membrane and protrudes extracellularly. This complex structure elucidates the molecular basis for the interaction between two essential components of a T4S system... - Secretion by numbers: Protein traffic in prokaryotesAnastasias Economou
Institute of Molecular Biology and Biotechnology, F O R T H and University of Crete, PO Box 1527, GR-711 10 Iraklio, Crete, Greece
Mol Microbiol 62:308-19. 2006....
Research Grants
- TRANSLOCATION OF DNA ACROSS THE AGROBACTERIUM ENVELOPEPeter Christie; Fiscal Year: 2007..The proposed studies will develop a mechanistic understanding of type IV secretion in unprecedented detail. ..
- DNA Translocation Across the Agrobacterium EnvelopePeter Christie; Fiscal Year: 2005....
- TRANSLOCATION OF DNA ACROSS THE AGROBACTERIUM ENVELOPEPeter Christie; Fiscal Year: 2001....
- TRANSLOCATION OF DNA ACROSS THE AGROBACTERIUM ENVELOPEPeter J Christie; Fiscal Year: 2010..The proposed studies will develop a mechanistic understanding of type IV secretion in unprecedented detail. ..