Wah Chiu

Summary

Affiliation: Baylor College of Medicine
Country: USA

Publications

  1. pmc A tail-like assembly at the portal vertex in intact herpes simplex type-1 virions
    Michael F Schmid
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas, United States of America
    PLoS Pathog 8:e1002961. 2012
  2. pmc Symmetry-free cryo-EM structures of the chaperonin TRiC along its ATPase-driven conformational cycle
    Yao Cong
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, National Center for Macromolecular Imaging, Baylor College of Medicine, Houston, TX 77030, USA
    EMBO J 31:720-30. 2012
  3. ncbi request reprint Structural biology of cellular machines
    Wah Chiu
    National Center for Macromolecular Imaging and Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Trends Cell Biol 16:144-50. 2006
  4. pmc Structural basis for scaffolding-mediated assembly and maturation of a dsDNA virus
    Dong Hua Chen
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 108:1355-60. 2011
  5. pmc Cryo-EM structure of a group II chaperonin in the prehydrolysis ATP-bound state leading to lid closure
    Junjie Zhang
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, National Center for Macromolecular Imaging, Baylor College of Medicine, Houston, TX 77030, USA
    Structure 19:633-9. 2011
  6. pmc Mechanism of folding chamber closure in a group II chaperonin
    Junjie Zhang
    Graduate Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, Houston, Texas 77030, USA
    Nature 463:379-83. 2010
  7. pmc Mechanism of lid closure in the eukaryotic chaperonin TRiC/CCT
    Christopher R Booth
    Graduate Program in Structural and Computational Biology and Molecular Biophysics, One Baylor Plaza, Baylor College of Medicine, Houston, Texas 77030, USA
    Nat Struct Mol Biol 15:746-53. 2008
  8. ncbi request reprint Architecture of the herpes simplex virus major capsid protein derived from structural bioinformatics
    Matthew L Baker
    National Center for Macromolecular Imaging, Baylor College of Medicine, Houston, TX 77030, USA
    J Mol Biol 331:447-56. 2003
  9. doi request reprint De novo backbone trace of GroEL from single particle electron cryomicroscopy
    Steven J Ludtke
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Structure 16:441-8. 2008
  10. ncbi request reprint An expanded conformation of single-ring GroEL-GroES complex encapsulates an 86 kDa substrate
    Dong Hua Chen
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Structure 14:1711-22. 2006

Detail Information

Publications96

  1. pmc A tail-like assembly at the portal vertex in intact herpes simplex type-1 virions
    Michael F Schmid
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas, United States of America
    PLoS Pathog 8:e1002961. 2012
    ..The location of this assembly suggests that it plays a role in genome release into the nucleus and is also important for virion architecture...
  2. pmc Symmetry-free cryo-EM structures of the chaperonin TRiC along its ATPase-driven conformational cycle
    Yao Cong
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, National Center for Macromolecular Imaging, Baylor College of Medicine, Houston, TX 77030, USA
    EMBO J 31:720-30. 2012
    ..Intriguingly, these mechanisms are different from other group I and II chaperonins despite their similar architecture...
  3. ncbi request reprint Structural biology of cellular machines
    Wah Chiu
    National Center for Macromolecular Imaging and Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Trends Cell Biol 16:144-50. 2006
    ..The establishment of a database describing the structural and dynamic properties of protein assemblies will provide novel opportunities to define the molecular and atomic mechanisms controlling overall cell physiology...
  4. pmc Structural basis for scaffolding-mediated assembly and maturation of a dsDNA virus
    Dong Hua Chen
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 108:1355-60. 2011
    ....
  5. pmc Cryo-EM structure of a group II chaperonin in the prehydrolysis ATP-bound state leading to lid closure
    Junjie Zhang
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, National Center for Macromolecular Imaging, Baylor College of Medicine, Houston, TX 77030, USA
    Structure 19:633-9. 2011
    ..This mechanism of structural changes in response to ATP is entirely different from those found in group I chaperonins...
  6. pmc Mechanism of folding chamber closure in a group II chaperonin
    Junjie Zhang
    Graduate Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, Houston, Texas 77030, USA
    Nature 463:379-83. 2010
    ....
  7. pmc Mechanism of lid closure in the eukaryotic chaperonin TRiC/CCT
    Christopher R Booth
    Graduate Program in Structural and Computational Biology and Molecular Biophysics, One Baylor Plaza, Baylor College of Medicine, Houston, Texas 77030, USA
    Nat Struct Mol Biol 15:746-53. 2008
    ..Our findings show how different mechanical switches can evolve from a common structural framework through modification of allosteric networks...
  8. ncbi request reprint Architecture of the herpes simplex virus major capsid protein derived from structural bioinformatics
    Matthew L Baker
    National Center for Macromolecular Imaging, Baylor College of Medicine, Houston, TX 77030, USA
    J Mol Biol 331:447-56. 2003
    ..The combined structural and sequence-based informatics has led to an architectural model of VP5. This model placed in the context of the capsid provides insights into the strategies used to achieve viral capsid stability...
  9. doi request reprint De novo backbone trace of GroEL from single particle electron cryomicroscopy
    Steven J Ludtke
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Structure 16:441-8. 2008
    ..This asymmetric conformation is different from previous asymmetric structures, including GroES-bound GroEL, and may represent a "primed state" in the chaperonin pathway...
  10. ncbi request reprint An expanded conformation of single-ring GroEL-GroES complex encapsulates an 86 kDa substrate
    Dong Hua Chen
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Structure 14:1711-22. 2006
    ..Encapsulation of the large assembly intermediate is supported by a series of electron cryomicroscopy studies as well as the protection of both alpha and beta subunits of the substrate from tryptic digestion...
  11. ncbi request reprint Fast rotational matching of single-particle images
    Yao Cong
    School of Health Information Sciences and Institute of Molecular Medicine, University of Texas Health Science Center at Houston, 7000 Fannin St, Suite 600, Houston, TX 77030, USA
    J Struct Biol 152:104-12. 2005
    ..Moreover, at sub-nanometer resolution, GroEL and rice dwarf virus reconstructions exhibit a remarkable resolution gain of 10-20% that is attributed to the novel image alignment kernel...
  12. pmc Subnanometer-resolution electron cryomicroscopy-based domain models for the cytoplasmic region of skeletal muscle RyR channel
    Irina I Serysheva
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 105:9610-5. 2008
    ..Taken together, these results provide a structural context for both channel gating and the consequences of certain malignant hyperthermia and central core disease-associated mutations in RyR1...
  13. pmc Location and flexibility of the unique C-terminal tail of Aquifex aeolicus co-chaperonin protein 10 as derived by cryo-electron microscopy and biophysical techniques
    Dong Hua Chen
    National Center for Macromolecular Imaging, Verna and Mars McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    J Mol Biol 381:707-17. 2008
    ..In addition, the 3D variance coefficient map identifies several regions inside the GroEL-Aacpn10 complex that have flexible conformations. This observation is in full agreement with the structural properties of an effective chaperonin...
  14. pmc Structure of the bifunctional and Golgi-associated formiminotransferase cyclodeaminase octamer
    Yuxin Mao
    Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX 77030, USA
    EMBO J 23:2963-71. 2004
    ..The structure further shed light on the molecular nature of two strong antigenic determinants of FTCD recognized by autoantibodies from patients with autoimmune hepatitis and on the binding of thin vimentin filaments to the FTCD octamer...
  15. pmc 4.0-A resolution cryo-EM structure of the mammalian chaperonin TRiC/CCT reveals its unique subunit arrangement
    Yao Cong
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemsitry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030
    Proc Natl Acad Sci U S A 107:4967-72. 2010
    ..These interior surface chemical properties likely play an important role in TRiC's cellular substrate specificity...
  16. pmc Validated near-atomic resolution structure of bacteriophage epsilon15 derived from cryo-EM and modeling
    Matthew L Baker
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 110:12301-6. 2013
    ..The presence of the canonical phage and jellyroll viral protein folds, gp7 and gp10, respectively, in the same virus suggests that epsilon15 may have emerged more recently relative to other bacteriophages. ..
  17. pmc Visualizing GroEL/ES in the act of encapsulating a folding protein
    Dong Hua Chen
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, National Center for Macromolecular Imaging, Baylor College of Medicine, Houston, TX 77030, USA
    Cell 153:1354-65. 2013
    ..A comparative structural analysis suggests that the allosteric transitions leading to substrate protein release and folding involve concerted shifts of GroES and the GroEL apical domains and C-terminal tails...
  18. pmc Reconstructing virus structures from nanometer to near-atomic resolutions with cryo-electron microscopy and tomography
    Juan Chang
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Adv Exp Med Biol 726:49-90. 2012
    ..Here we present the common techniques used to acquire and process cryo-EM and cryo-ET data and discuss their implications for structural virology both now and in the future...
  19. pmc Modeling protein structure at near atomic resolutions with Gorgon
    Matthew L Baker
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    J Struct Biol 174:360-73. 2011
    ..Examples from cryo-EM maps of Rotavirus and Rice Dwarf Virus are used to demonstrate its applicability to modeling protein structure...
  20. pmc Crystallographic conformers of actin in a biologically active bundle of filaments
    Yao Cong
    National Center for Macromolecular Imaging and Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    J Mol Biol 375:331-6. 2008
    ..The scruin and bundle packing forces appear to influence the tertiary and quaternary conformations of actin in the filament of this biologically active bundle...
  21. pmc JADAS: a customizable automated data acquisition system and its application to ice-embedded single particles
    Junjie Zhang
    Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, Houston, TX 77030, USA
    J Struct Biol 165:1-9. 2009
    ....
  22. pmc 4.4 Å cryo-EM structure of an enveloped alphavirus Venezuelan equine encephalitis virus
    Rui Zhang
    Graduate Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, Houston, TX, USA
    EMBO J 30:3854-63. 2011
    ..Our structural results suggest a mechanism for the initial stage of nucleocapsid core formation, and shed light on the virulence attenuation, host recognition and neutralizing activities of VEEV and other alphavirus pathogens...
  23. pmc Structural changes in a marine podovirus associated with release of its genome into Prochlorococcus
    Xiangan Liu
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas, USA
    Nat Struct Mol Biol 17:830-6. 2010
    ..Our observations suggest a mechanism whereby, upon binding to the host cell, the tail fibers induce a cascade of structural alterations of the portal vertex complex that triggers DNA release...
  24. ncbi request reprint Automated segmentation of molecular subunits in electron cryomicroscopy density maps
    Matthew L Baker
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    J Struct Biol 156:432-41. 2006
    ..As such, automated segmentation could become a useful tool for the analysis of the ever-increasing number of structures of macromolecular machines derived from cryoEM...
  25. pmc Zernike phase contrast cryo-electron microscopy and tomography for structure determination at nanometer and subnanometer resolutions
    Kazuyoshi Murata
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Structure 18:903-12. 2010
    ..These results show quantitatively the benefits of ZPC-cryoEM and ZPC-cryoET for structural determinations of macromolecular machines at nanometer and subnanometer resolutions...
  26. pmc The pore structure of the closed RyR1 channel
    Steven J Ludtke
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Structure 13:1203-11. 2005
    ..The kinked inner helix closely resembles the inner helix of the open MthK channel, suggesting that kinking alone does not open RyR1, as proposed for K+ channels...
  27. pmc Structure of epsilon15 bacteriophage reveals genome organization and DNA packaging/injection apparatus
    Wen Jiang
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Nature 439:612-6. 2006
    ..The image reconstruction approach should be applicable to studying other biological nanomachines with components of mixed symmetries...
  28. pmc Visualizing virus assembly intermediates inside marine cyanobacteria
    Wei Dai
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Nature 502:707-10. 2013
    ..The morphogenetic pathway we describe here is highly conserved and was probably established long before that of double-stranded DNA viruses infecting more complex organisms. ..
  29. pmc Tracing conformational changes in proteins
    Nurit Haspel
    Department of Computer Science, Rice University, Houston, TX 77005, USA
    BMC Struct Biol 10:S1. 2010
    ..Traditional biophysics-based conformational search methods require a large number of calculations and are hard to apply to large-scale conformational motions...
  30. ncbi request reprint Cryo-EM asymmetric reconstruction of bacteriophage P22 reveals organization of its DNA packaging and infecting machinery
    Juan Chang
    Graduate Program in Structural and Computational Biology and Molecular Biophysics, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Structure 14:1073-82. 2006
    ..The coaxially packed DNA genome is encapsidated by the icosahedral shell. This complete structure unifies various biochemical, genetic, and crystallographic data of its components from the past several decades...
  31. pmc Ab initio modeling of the herpesvirus VP26 core domain assessed by CryoEM density
    Matthew L Baker
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas, United States of America
    PLoS Comput Biol 2:e146. 2006
    ..While this new model provides for a better understanding of the assembly and interactions of VP26 in HSV-1, the approach itself may have broader applications in modeling the components of large macromolecular assemblies...
  32. pmc Cryo-EM of macromolecular assemblies at near-atomic resolution
    Matthew L Baker
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas, USA
    Nat Protoc 5:1697-708. 2010
    ..Additionally, the feature recognition and computational modeling tools can be applied to any near-atomic resolution density maps, including those from X-ray crystallography...
  33. ncbi request reprint The structure of ClpB: a molecular chaperone that rescues proteins from an aggregated state
    Sukyeong Lee
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Cell 115:229-40. 2003
    ..Taken together, we propose a mechanism by which an ATP-driven conformational change is coupled to a large coiled-coil motion, which is indispensable for protein disaggregation...
  34. ncbi request reprint Structure of the type 1 inositol 1,4,5-trisphosphate receptor revealed by electron cryomicroscopy
    Irina I Serysheva
    Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA
    J Biol Chem 278:21319-22. 2003
    ..A structural mapping of the amino acid sequences to several functional domains is deduced within the structure of the InsP3R1 tetramer...
  35. pmc Achievable resolution from images of biological specimens acquired from a 4k x 4k CCD camera in a 300-kV electron cryomicroscope
    Dong Hua Chen
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    J Struct Biol 163:45-52. 2008
    ....
  36. ncbi request reprint Seeing GroEL at 6 A resolution by single particle electron cryomicroscopy
    Steven J Ludtke
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030 USA
    Structure 12:1129-36. 2004
    ..The 6 A resolution cryo-EM GroEL structure clearly demonstrates the veracity and expanding scope of cryo-EM and the single particle reconstruction technique for macromolecular machines...
  37. pmc Constructing and validating initial Cα models from subnanometer resolution density maps with pathwalking
    Mariah R Baker
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Structure 20:450-63. 2012
    ..With the growing number of near-atomic resolution density maps from cryo-EM and X-ray crystallography, pathwalking can become an important tool in modeling protein structures...
  38. pmc Structural mechanism of SDS-induced enzyme activity of scorpion hemocyanin revealed by electron cryomicroscopy
    Yao Cong
    National Center for Macromolecular Imaging, Baylor College of Medicine, Houston, TX 77030, USA
    Structure 17:749-58. 2009
    ..This mechanism could be applicable to other type 3 copper proteins, as the active site is highly conserved...
  39. ncbi request reprint Modular software platform for low-dose electron microscopy and tomography
    Michael P Marsh
    Graduate Program in Structural and Computational Biology and Molecular Biophysics, and National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    J Microsc 228:384-9. 2007
    ..Both applications have been widely used within our NCRR-supported Center for routine data collection and are now made available for public download...
  40. ncbi request reprint Experimental verification of conformational variation of human fatty acid synthase as predicted by normal mode analysis
    Jacob Brink
    National Center for Macromolecular Imaging, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Structure 12:185-91. 2004
    ....
  41. pmc Electron cryotomography reveals the portal in the herpesvirus capsid
    Juan T Chang
    Graduate Program in Structural and Computational Biology and Molecular Biophysics, National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    J Virol 81:2065-8. 2007
    ..Such similarity in portal organization supports the idea of the evolutionary relatedness of these viruses...
  42. ncbi request reprint Learning-based segmentation framework for tissue images containing gene expression data
    Musodiq Bello
    Computational Biomedicine Lab, University of Houston, Houston, TX 77204 3010, USA
    IEEE Trans Med Imaging 26:728-44. 2007
    ..92 compared with expert segmentation in 64 images tested. This tool is intended to help scientists interpret large-scale gene expression patterns more efficiently...
  43. pmc Structure of Trypanosoma brucei flagellum accounts for its bihelical motion
    Alexey Y Koyfman
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 108:11105-8. 2011
    ..We propose that the PFR modifies the in-plane axoneme motion to produce the characteristic trypanosome bihelical motility as captured by high-speed light microscope videography...
  44. pmc Direct electron detection yields cryo-EM reconstructions at resolutions beyond 3/4 Nyquist frequency
    Benjamin E Bammes
    Graduate Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, Houston, TX 77030, USA
    J Struct Biol 177:589-601. 2012
    ..We anticipate the unparalleled performance of this digital recording device will dramatically benefit cryo-EM for routine tomographic and single-particle structural determination of biological specimens...
  45. pmc Near-atomic-resolution cryo-EM for molecular virology
    Corey F Hryc
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Curr Opin Virol 1:110-7. 2011
    ..This paper reviews the recent advances in near-atomic-resolution cryo-EM for probing the mechanisms of virus assembly and morphogenesis...
  46. pmc Gorgon and pathwalking: macromolecular modeling tools for subnanometer resolution density maps
    Matthew L Baker
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, National Center for Macromolecular Imaging, Baylor College of Medicine, Houston, TX 77030, USA
    Biopolymers 97:655-68. 2012
    ..In the end, the utilities that we present here offer users a robust toolkit for analyzing and modeling protein structure in macromolecular assemblies at non-atomic resolutions...
  47. ncbi request reprint Applications of a bilateral denoising filter in biological electron microscopy
    Wen Jiang
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    J Struct Biol 144:114-22. 2003
    ..In as much, we have applied this technique to individual micrographs, entire 3D reconstructions, segmented proteins, and tomographic reconstructions...
  48. pmc Structure of the herpesvirus major capsid protein
    Brian R Bowman
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Graduate Program in Structural and Computational Biology, Baylor College of Medicine, Houston, TX 77030, USA
    EMBO J 22:757-65. 2003
    ..These differences may mediate the binding and release of other structural proteins during capsid maturation...
  49. ncbi request reprint Coat protein fold and maturation transition of bacteriophage P22 seen at subnanometer resolutions
    Wen Jiang
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    Nat Struct Biol 10:131-5. 2003
    ..The cryo-EM shows that a large conformational change of the P22 capsid during maturation transition involves not only the domain movement of individual subunits, but also refolding of the capsid protein...
  50. pmc The skeletal muscle Ca2+ release channel has an oxidoreductase-like domain
    Matthew L Baker
    Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 99:12155-60. 2002
    ..Consistent with the prediction of an oxidoreductase domain, RyR1 binds [3H]NAD+, supporting a model in which RyR1 has a oxidoreductase-like domain that could function as a type of redox sensor...
  51. pmc Visualizing the structural changes of bacteriophage Epsilon15 and its Salmonella host during infection
    Juan T Chang
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    J Mol Biol 402:731-40. 2010
    ..This tube would direct the DNA into the cytoplasm and protect it from periplasmic nucleases. Once the DNA has been injected into the cell, the tube and portal seals, and the empty bacteriophage remains at the cell surface...
  52. pmc Common ancestry of herpesviruses and tailed DNA bacteriophages
    Matthew L Baker
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    J Virol 79:14967-70. 2005
    ..On the basis of this and other emerging relationships, it seems increasingly likely that the very diverse collection of extant viruses may have arisen from a relatively small number of primordial progenitors...
  53. ncbi request reprint Assessing the capabilities of a 4kx4k CCD camera for electron cryo-microscopy at 300kV
    Christopher R Booth
    National Center for Macromolecular Imaging, Baylor College of Medicine, Houston, TX 77030, USA
    J Struct Biol 156:556-63. 2006
    ..Based on this analysis it is realistic to collect images containing subnanometer resolution data (6-9A) using this CCD camera at an effective magnification of approximately 112000x on a 300kV electron microscope...
  54. pmc Structure of Ca2+ release channel at 14 A resolution
    Irina I Serysheva
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    J Mol Biol 345:427-31. 2005
    ..This putative structure was computationally localized to the clamp-shaped region of RyR1, which has been implicated to have a regulatory role in the channel activity...
  55. ncbi request reprint Electron cryomicroscopy of biological machines at subnanometer resolution
    Wah Chiu
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Structure 13:363-72. 2005
    ..The consequences of these analyses can not only identify accurately some of the secondary structure elements of the molecular components in machines but also suggest structural mechanisms related to their biological functions...
  56. ncbi request reprint A 9 angstroms single particle reconstruction from CCD captured images on a 200 kV electron cryomicroscope
    Christopher R Booth
    Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, Houston, TX 77030, USA
    J Struct Biol 147:116-27. 2004
    ....
  57. pmc Identification of secondary structure elements in intermediate-resolution density maps
    Matthew L Baker
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Structure 15:7-19. 2007
    ....
  58. pmc EMEN2: an object oriented database and electronic lab notebook
    Ian Rees
    Graduate Program of Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, Houston, TX 77030, USA
    Microsc Microanal 19:1-10. 2013
    ..EMEN2 installations currently support roughly 800 scientists worldwide with over 1/2 million experimental records and over 20 TB of experimental data. The software is freely available with complete source...
  59. pmc Radiation damage effects at four specimen temperatures from 4 to 100 K
    Benjamin E Bammes
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    J Struct Biol 169:331-41. 2010
    ..At lower resolution, suitable for tomography, intermediate temperatures (25 or 42K) may provide a modest improvement in cryo-protection without introducing deleterious effects evident at 4 K...
  60. pmc Structure of Halothiobacillus neapolitanus carboxysomes by cryo-electron tomography
    Michael F Schmid
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    J Mol Biol 364:526-35. 2006
    ..The averages reveal icosahedral symmetry of the shell, but not of the density inside it, for all the size classes...
  61. pmc Interprotofilament interactions between Alzheimer's Abeta1-42 peptides in amyloid fibrils revealed by cryoEM
    Rui Zhang
    Graduate Program in Structural and Computational Biology and Molecular Biophysics, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 106:4653-8. 2009
    ..In our model, the C terminus of Abeta forms the inside wall of the hollow core, which is supported by partial proteolysis analysis...
  62. pmc Cryoelectron microscopy of protein IX-modified adenoviruses suggests a new position for the C terminus of protein IX
    Michael P Marsh
    Program in Structural and Computational Biology and Molecular Biophysics, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
    J Virol 80:11881-6. 2006
    ..The original assignment of IIIa was based largely on indirect evidence, and the data presented herein support the reassignment of the IIIa density as protein IX...
  63. ncbi request reprint Single-particle electron cryomicroscopy of the ion channels in the excitation-contraction coupling junction
    Irina I Serysheva
    National Center for Macromolecular Imaging, Baylor College of Medicine, Houston, Texas 77030, USA
    Methods Cell Biol 79:407-35. 2007
  64. pmc Workshop on molecular animation
    Sarina Bromberg
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Structure 18:1261-5. 2010
    ..Another use of molecular animation emerged in the workshop: helping to focus scientific inquiry about the motions of molecules and enhancing informal communication within and between laboratories...
  65. pmc Flexible architecture of IP3R1 by Cryo-EM
    Steven J Ludtke
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Structure 19:1192-9. 2011
    ..3D statistical analysis of the cryo-EM density map identifies high variance in the cytoplasmic region. This structural variation could be attributed to genuine structural flexibility of IP3R1...
  66. pmc Comparison of Segger and other methods for segmentation and rigid-body docking of molecular components in cryo-EM density maps
    Grigore Pintilie
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Biopolymers 97:742-60. 2012
    ..The accuracy of the component segmentation using Segger and other methods was assessed by comparing segmented regions with docked components...
  67. pmc Averaging tens to hundreds of icosahedral particle images to resolve protein secondary structure elements using a Multi-Path Simulated Annealing optimization algorithm
    Xiangan Liu
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    J Struct Biol 160:11-27. 2007
    ..6A resolution at which four helices of the P3A subunit of RDV are resolved. Furthermore, with the 284 best particles, the reconstruction is improved to 7.9A resolution, and 21 of 22 helices and six of seven beta sheets are resolved...
  68. ncbi request reprint Focal pair merging for contrast enhancement of single particles
    Steven J Ludtke
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    J Struct Biol 144:73-8. 2003
    ..This technique is useful in numerous cases where low-contrast images are produced, such as small particles, proteins solubilized in detergent or projects with high-resolution goals when the first image is taken very close to focus...
  69. ncbi request reprint Conformational changes in Sindbis virions resulting from exposure to low pH and interactions with cells suggest that cell penetration may occur at the cell surface in the absence of membrane fusion
    Angel M Paredes
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Virology 324:373-86. 2004
    ..These data suggest an alternative route of infection for Sindbis virus that occurs by a process that does not involve membrane fusion and does not require disassembly of the virus protein shell...
  70. pmc Practical performance evaluation of a 10k × 10k CCD for electron cryo-microscopy
    Benjamin E Bammes
    Graduate Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, Houston, TX 77030, USA
    J Struct Biol 175:384-93. 2011
    ..emdatabank.org/)...
  71. ncbi request reprint Electron beam coater for reduction of charging in ice-embedded biological specimens using Ti(88)Si(12) alloy
    Michael B Sherman
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Microsc Microanal 9:566-73. 2003
    ..Images of the crystals after coating showed diffraction spots of up to 3 A resolution...
  72. ncbi request reprint Deriving folds of macromolecular complexes through electron cryomicroscopy and bioinformatics approaches
    Wah Chiu
    Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, Houston, Texas 77030, USA
    Curr Opin Struct Biol 12:263-9. 2002
    ..Such approaches have been demonstrated with the 6.8 A structure of the rice dwarf virus to derive models for the major capsid shell proteins...
  73. ncbi request reprint Determination of icosahedral virus structures by electron cryomicroscopy at subnanometer resolution
    Z Hong Zhou
    Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, Houston, Texas 77030, USA
    Adv Protein Chem 64:93-124. 2003
  74. ncbi request reprint Object oriented database and electronic notebook for transmission electron microscopy
    Steven J Ludtke
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Microsc Microanal 9:556-65. 2003
    ..Finally, as an object-oriented database, the database structure is dynamic and can be easily extended to incorporate information not defined in the original database specification...
  75. pmc Partially polymerized liposomes: stable against leakage yet capable of instantaneous release for remote controlled drug delivery
    Guoting Qin
    Department of Radiology, The Methodist Hospital Research Institute, Houston, TX 77030, USA
    Nanotechnology 22:155605. 2011
    ..The remote activation feature of this novel drug delivery system allows for precise temporal and spatial control of drug release...
  76. ncbi request reprint Structure of the acrosomal bundle
    Michael F Schmid
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Nature 431:104-7. 2004
    ....
  77. pmc A digital atlas to characterize the mouse brain transcriptome
    James P Carson
    Program in Structural and Computational Biology and Molecular Biophysics, National Center for Macromolecular Imaging, Baylor College of Medicine, Houston, Texas, United States of America
    PLoS Comput Biol 1:e41. 2005
    ..The semi-automated annotation method developed here is applicable to a broad spectrum of complex tissues and data modalities...
  78. ncbi request reprint Structure of triglyceride-rich human low-density lipoproteins according to cryoelectron microscopy
    Michael B Sherman
    Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
    Biochemistry 42:14988-93. 2003
    ..This effect is likely achieved through changes in the conformation of apo-B-100. These data suggest that the physical state of the LDL core determines particle shape, surface structure, and metabolic fate...
  79. pmc Quaternary structure of human fatty acid synthase by electron cryomicroscopy
    Jacob Brink
    Verna and Marrs McLean Department of Biochemistry and Molecular Biology and National Center for Macromolecular Imaging, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 99:138-43. 2002
    ..Each monomer subunit appears to be subdivided into three structural domains. With this reconstruction of the synthase, we propose a location for the enzyme's two fatty acid synthesis sites...
  80. ncbi request reprint High resolution structural studies of complex icosahedral viruses: a brief overview
    Wah Chiu
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA
    Virus Res 82:9-17. 2002
    ..3 MDa per asymmetric unit. The structural results reveal that each of these proteins has distinct folds and they are packed uniquely to form stable particles...
  81. pmc Structure of isolated nucleocapsids from venezuelan equine encephalitis virus and implications for assembly and disassembly of enveloped virus
    Angel Paredes
    National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, Texas 77030, USA
    J Virol 77:659-64. 2003
    ....
  82. pmc Seeing the portal in herpes simplex virus type 1 B capsids
    R H Rochat
    Structural Computational Biology and Molecular Biophysics Program, Baylor College of Medicine, Houston, Texas 77030, USA
    J Virol 85:1871-4. 2011
    ..Our map unequivocally resolves the 12-subunit portal situated beneath one of the pentameric vertices, thus removing uncertainty over the location and stoichiometry of the herpesvirus portal...
  83. pmc Protein structure fitting and refinement guided by cryo-EM density
    Maya Topf
    School of Crystallography, Birkbeck College, University of London, Malet Street, London WC1E 7HX, United Kingdom
    Structure 16:295-307. 2008
    ..At approximately 10 A resolution, Calpha rmsd between the initial and final structures was reduced on average by approximately 53%. The method is automated and can refine both experimental and predicted atomic structures...
  84. ncbi request reprint DNA packaging and delivery machines in tailed bacteriophages
    John E Johnson
    Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
    Curr Opin Struct Biol 17:237-43. 2007
    ....
  85. ncbi request reprint Electron cryo-microscopy of VAT, the archaeal p97/CDC48 homologue from Thermoplasma acidophilum
    Beate Rockel
    Max Planck Institut fur Biochemie, Abteilung Molekulare Strukturbiologie, Am Klopferspitz 18 A, 82152 Martinsried, Germany
    J Mol Biol 317:673-81. 2002
    ..Comparison with the p97 3-D reconstruction and the recently determined crystal structure of p97-N-D1 suggests a tail-to-tail arrangement of D1 and D2 in VAT...
  86. ncbi request reprint Backbone structure of the infectious epsilon15 virus capsid revealed by electron cryomicroscopy
    Wen Jiang
    Markey Center for Structural Biology, Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    Nature 451:1130-4. 2008
    ..Beyond epsilon15, this method potentially offers a new approach for modelling the backbone conformations of the protein subunits in other macromolecular assemblies at near-native solution states...
  87. ncbi request reprint Cryoelectron microscopy of icosahedral virus particles
    Wen Jiang
    Department of Biological Sciences, Purdue Univesity, IN, USA
    Methods Mol Biol 369:345-63. 2007
    ..In addition, we will describe how to extract useful structural information from the three-dimensional maps...
  88. ncbi request reprint Structural analysis of the anaphase-promoting complex reveals multiple active sites and insights into polyubiquitylation
    Lori A Passmore
    Section of Structural Biology, The Institute of Cancer Research, Chester Beatty Laboratories, London, UK
    Mol Cell 20:855-66. 2005
    ..Our data suggest that multimerisation and/or the presence of multiple active sites facilitates the APC/C's ability to elongate polyubiquitin chains...
  89. ncbi request reprint Mitochondrial ATP synthasome: three-dimensional structure by electron microscopy of the ATP synthase in complex formation with carriers for Pi and ADP/ATP
    Chen Chen
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2185, USA
    J Biol Chem 279:31761-8. 2004
    ..Collectively, these finding support a mechanism in which the entry of the substrates ADP and P(i) into mitochondria, the synthesis of ATP on F(1), and the release and exit of ATP are very localized and highly coordinated events...
  90. ncbi request reprint Hybrid segmentation framework for tissue images containing gene expression data
    Musodiq Bello
    Computational Biomedicine Lab, Dept of Computer Science, University of Houston, Houston TX, USA
    Med Image Comput Comput Assist Interv 8:254-61. 2005
    ..The automated large-scale annotation will help scientists interpret gene expression patterns at cellular resolution more efficiently...
  91. ncbi request reprint Close membrane-membrane proximity induced by Ca(2+)-dependent multivalent binding of synaptotagmin-1 to phospholipids
    Demet Arac
    Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
    Nat Struct Mol Biol 13:209-17. 2006
    ..We propose a model wherein synaptotagmin cooperates with the SNAREs in bringing the synaptic vesicle and plasma membranes together and accelerates membrane fusion through the highly positive electrostatic potential of its C(2)B domain...
  92. ncbi request reprint Refinement of protein structures by iterative comparative modeling and CryoEM density fitting
    Maya Topf
    Department of Biopharmaceutical Sciences, California Institute for Quantitative Biomedical Research, QB3, 1700 4th Street, Suite 503B, University of California at San Francisco, San Francisco, CA 94143 2552, USA
    J Mol Biol 357:1655-68. 2006
    ..The method is being implemented in our program MODELLER for protein structure modeling by satisfaction of spatial restraints and will be applicable to the rapidly increasing number of cryoEM density maps of macromolecular assemblies...
  93. pmc Genome sequence, structural proteins, and capsid organization of the cyanophage Syn5: a "horned" bacteriophage of marine synechococcus
    Welkin H Pope
    Department of Biology, Massachusetts Institute of Technology, Cambridge MA 02139, USA
    J Mol Biol 368:966-81. 2007
    ..This phage/host system should allow detailed investigation of the physiology and biochemistry of phage propagation in marine photosynthetic bacteria...
  94. ncbi request reprint Structural characterization of components of protein assemblies by comparative modeling and electron cryo-microscopy
    Maya Topf
    Department of Biopharmaceutical Sciences, California Institute for Quantitative Biomedical Research, Mission Bay Genentech Hall, 600 16th Street, Suite N472D, University of California, San Francisco, CA 94143, USA
    J Struct Biol 149:191-203. 2005
    ..Moreover, a pseudo-atomic model of a component in an assembly may be built better with comparative models of the native subunit sequences than with experimentally determined structures of their homologs...
  95. ncbi request reprint 3D volume reconstruction of a mouse brain from histological sections using warp filtering
    Tao Ju
    Washington University, St Louis, MO, USA
    J Neurosci Methods 156:84-100. 2006
    ..Finally, a validation framework is proposed and applied to evaluate the quality of reconstruction using both real sections and a synthetic volume...

Research Grants30

  1. Unified Data Resource for Large Complexes Determined by Cryo-Electron Microscopy
    Wah Chiu; Fiscal Year: 2010
    ..data collection, map generation, cryoEM map restrained modeling, visualization, segmentation and annotation (Wah Chiu)...
  2. Icosahedral Particle Reconstruction Software
    Wah Chiu; Fiscal Year: 2004
    ..The experimental data consist of structures that we have previously determined to subnanometer resolution and for which the crystal structures of some of their components are known. ..
  3. STRUCTURAL STUDIES OF HERPESVIRUS CAPSIDS
    Wah Chiu; Fiscal Year: 2005
    ..The outcome of our investigations will provide insights on the structural assembly of this important human pathogen and also advance the structural approaches for studying large macromolecular machines. ..
  4. Icosahedral Particle Reconstruction Software
    Wah Chiu; Fiscal Year: 2005
    ..The experimental data consist of structures that we have previously determined to subnanometer resolution and for which the crystal structures of some of their components are known. ..
  5. Center for Protein Folding Machinery (RMI)
    Wah Chiu; Fiscal Year: 2007
    ..Finally, the organization, collaborations and communications in our NDC exemplify the 21st century goal of conducting interdisciplinary research via new mechanisms of data sharing and analysis. ..
  6. Unified Data Resource for Large Complexes Determined by Cryo-Electron Microscopy
    Wah Chiu; Fiscal Year: 2007
    ..data collection, map generation, cryoEM map restrained modeling, visualization, segmentation and annotation (Wah Chiu)...
  7. Structures of the portal vertex in dsDNA viruses and virus-infected cells
    Wah Chiu; Fiscal Year: 2009
    ..The proposed studies, when combined with other biological and modeling information, will yield valuable structural information for developing anti-viral and gene delivery strategies targeted at these processes. ..
  8. Structures of the portal vertex in dsDNA viruses and virus-infected cells
    Wah Chiu; Fiscal Year: 2010
    ..The proposed studies, when combined with other biological and modeling information, will yield valuable structural information for developing anti-viral and gene delivery strategies targeted at these processes. ..
  9. STRUCTURAL STUDIES OF HERPESVIRUS CAPSIDS
    Wah Chiu; Fiscal Year: 2004
    ..The outcome of our investigations will provide insights on the structural assembly of this important human pathogen and also advance the structural approaches for studying large macromolecular machines. ..
  10. STRUCTURAL BASIS OF BACTERIOPHAGE P22 ASSEMBLY
    Wah Chiu; Fiscal Year: 2000
    ....
  11. STRUCTURAL STUDIES OF HERPESVIRUS CAPSIDS
    Wah Chiu; Fiscal Year: 2001
    ..The outcome of our investigations will provide insights on the structural assembly of this important human pathogen and also advance the structural approaches for studying large macromolecular machines. ..
  12. STRUCTURAL BASIS OF BACTERIOPHAGE P22 ASSEMBLY
    Wah Chiu; Fiscal Year: 2001
    ....
  13. STRUCTURAL STUDIES OF HERPESVIRUS CAPSIDS
    Wah Chiu; Fiscal Year: 1999
    ..We anticipate that this research will not only advance a useful tool or virus structure research but also shed critical insights into structure-based mechanism of biological activities of herpesvirus capsids. ..
  14. STRUCTURAL STUDIES OF HERPESVIRUS CAPSIDS
    Wah Chiu; Fiscal Year: 2002
    ..The outcome of our investigations will provide insights on the structural assembly of this important human pathogen and also advance the structural approaches for studying large macromolecular machines. ..
  15. STRUCTURAL BASIS OF BACTERIOPHAGE P22 ASSEMBLY
    Wah Chiu; Fiscal Year: 2002
    ....
  16. STRUCTURAL STUDIES OF HERPESVIRUS CAPSIDS
    Wah Chiu; Fiscal Year: 2003
    ..The outcome of our investigations will provide insights on the structural assembly of this important human pathogen and also advance the structural approaches for studying large macromolecular machines. ..
  17. Unified Data Resource for Large Complexes Determined by Cryo-Electron Microscopy
    Wah Chiu; Fiscal Year: 2009
    ..data collection, map generation, cryoEM map restrained modeling, visualization, segmentation and annotation (Wah Chiu)...
  18. STRUCTURAL BASIS OF BACTERIOPHAGE P22 ASSEMBLY
    Wah Chiu; Fiscal Year: 1999
    ....
  19. High throughput icosahedral particle reconstruction
    Wah Chiu; Fiscal Year: 2003
    ..abstract_text> ..
  20. Computation Center for Biomolecular Complexes (RMI)
    Wah Chiu; Fiscal Year: 2006
    ..We will have a project manager to organize the workshops and communications among different investigators and institutions. There will be an external advisory committee to guide the formation of the proposed center. ..
  21. Icosahedral Particle Reconstruction Software
    Wah Chiu; Fiscal Year: 2006
    ..The experimental data consist of structures that we have previously determined to subnanometer resolution and for which the crystal structures of some of their components are known. ..