Research Topics
| William E BrownellSummaryAffiliation: Baylor College of Medicine Country: USA Publications
Research Grants
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Detail Information
Publications
Micro- and nanomechanics of the cochlear outer hair cellW E Brownell
Bobby R Alford Department of Otorhinolaryngology and Communicative Sciences, Baylor College of Medicine and Department of Bioengineering, Rice University, Houston, Texas 77030, USA
Annu Rev Biomed Eng 3:169-94. 2001..The methods and techniques utilized to increase our understanding of the central role outer hair cell mechanics plays in hearing are also relevant to broader research questions in cell mechanics, cell motility, and cell transduction...
Membrane cholesterol modulates cochlear electromechanicsWilliam E Brownell
Bobby R Alford Department of Otolaryngology Head and Neck Surgery, Baylor College of Medicine, Houston, TX 77030, USA
Pflugers Arch 461:677-86. 2011..The enhanced electromechanical response reflects an increase in outer hair cell electromotility and may reveal features of cholesterol distribution and trafficking in outer hair cells...- Cell membrane tethers generate mechanical force in response to electrical stimulationWilliam E Brownell
Bobby R Alford Department of Otolaryngology, Head and Neck Surgery, and Department of Neuroscience, Baylor College of Medicine, Houston, Texas, USA
Biophys J 99:845-52. 2010..By harnessing the energy in the transmembrane electric field, cell membranes may contribute to processes as diverse as outer hair cell electromotility, ion channel gating, and transport... - Poking versus deflection: anisotropy in actionWilliam E Brownell
Department of Otolaryngology, Baylor College of Medicine, Houston, Texas, USA
Biophys J 94:4157-8. 2008 - Glycosylation regulates prestin cellular activityLavanya Rajagopalan
Bobby R Alford Department of Otolaryngology Head and Neck Surgery, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
J Assoc Res Otolaryngol 11:39-51. 2010..We speculate that the cholesterol regulation of prestin occurs through localization to and internalization from membrane microdomains by clathrin- and caveolin-dependent mechanisms... - Membrane composition modulates prestin-associated charge movementJohn Sfondouris
Bobby R Alford Department of Otolaryngology Head and Neck Surgery, Baylor College of Medicine, Houston, Texas 77030, USA
J Biol Chem 283:22473-81. 2008..Our results quantify the relation between membrane cholesterol concentration and prestin-associated charge movement and enhance our understanding of how membrane composition modulates prestin function... - Tuning of the outer hair cell motor by membrane cholesterolLavanya Rajagopalan
Bobby R Alford Department of Otolaryngology Head and Neck Surgery, Huffington Center on Aging and Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA
J Biol Chem 282:36659-70. 2007..These findings indicate that alterations in membrane cholesterol affect prestin function and functionally tune the outer hair cell... - Prestin modulates mechanics and electromechanical force of the plasma membraneRui Zhang
Applied Physics Graduate Program, Rice University, Houston, Texas, USA
Biophys J 93:L07-9. 2007..These results suggest that prestin-associated charge transfer is necessary for maximal EMF generation by the membrane... - Effects of chlorpromazine on mechanical properties of the outer hair cell plasma membraneDavid R Murdock
Department of Bioengineering, Rice University, Houston, Texas 77251-1892, USA
Biophys J 89:4090-5. 2005..The CPZ-induced changes in tether viscoelastic behavior suggest that alterations in the mechanical properties of the OHC lateral wall could play a role in the modulation of OHC electromotility by CPZ... - Studies of plasma membrane mechanics and plasma membrane-cytoskeleton interactions using optical tweezers and fluorescence imagingSergey A Ermilov
Department of Bioengineering, Rice University, P O Box 1892, MS 142, Houston, TX 77251 1892, USA
J Biomech 40:476-80. 2007.... - Effects of salicylate on plasma membrane mechanicsSergey A Ermilov
Department of Bioengineering, Rice University, P.O. Box 1892, MS 142, Houston, Texas 77251-1892, USA
J Neurophysiol 94:2105-10. 2005.... - Tonotopic relationships reveal the charge density varies along the lateral wall of outer hair cellsChristian Corbitt
Bobby R Alford Department of Otolaryngology Head and Neck Surgery, Baylor College of Medicine, Houston, Texas, USA
Biophys J 102:2715-24. 2012..3/A(LW) and ρ(LW) = 9/A(LW)). This shows contrary to conventional wisdom that σ(LW) and ρ(LW) are not constant along the length of an individual outer hair cell... - Standing wave total internal reflection fluorescence microscopy to measure the size of nanostructures in living cellsOlga Gliko
Department of Otolaryngology-Head and Neck Surgery, Baylor College of Medicine, Houston, Texas 77030, USA
J Biomed Opt 11:064013. 2006..The combination of SWFM with total internal reflection (TIR), referred to as SW-TIRFM, allows additional improvement of axial resolution by selective excitation of fluorescence in a layer of about 100 nm... - Hypotonic swelling of salicylate-treated cochlear outer hair cellsMan Zhi
Bobby R Alford Department of Otolaryngology Head and Neck Surgery, Baylor College of Medicine, Houston, TX 77030, United States
Hear Res 228:95-104. 2007.... - Fast two-dimensional standing-wave total-internal-reflection fluorescence microscopy using acousto-optic deflectorsOlga Gliko
Department of Otorhinolaryngology Head and Neck Surgery, Baylor College of Medicine, Houston, TX 77030, USA
Opt Lett 34:836-8. 2009..This technique can provide real-time imaging of subresolution structures in live biological specimens near a glass-water interface... - Dyslipidemia and auditory functionM Bradley Evans
Bobby R. Alford Department of Otolaryngology-Head and Neck Surgery, Baylor College of Medicine, Houston, Texas 77030, USA
Otol Neurotol 27:609-14. 2006..These results suggest that whereas chronic dyslipidemia associated with elevated triglycerides may reduce auditory function, short-term dietary changes may not... - Excess plasma membrane and effects of ionic amphipaths on mechanics of outer hair cell lateral wallNoriko Morimoto
Department of Otorhinolaryngology and Communicative Science, Baylor College of Medicine, Houston, Texas 77030, USA
Am J Physiol Cell Physiol 282:C1076-86. 2002..Application of CPZ and/or Sal decreased this strain-induced hydraulic conductivity. The impact of ionic amphipaths on OHC plasma membrane and lateral wall mechanics may contribute to their effects on OHC electromotility and hearing... - Photometric recording of transmembrane potential in outer hair cellsTakashi Nakagawa
Bobby R Alford Department of Otolaryngology-Head and Neck Surgery, Baylor College of Medicine, Houston, TX 77030, USA
J Neural Eng 3:79-86. 2006..Results are consistent with the hypothesis that OHCs exhibit inhomogeneous membrane potentials that vary with position in analogy with the voltage in nerve axons... - Measuring forces at the leading edge: a force assay for cell motilityBrenda Farrell
Bobby R Alford Department of Otolaryngology Head and Neck Surgery, Baylor College of Medicine, Houston, TX 77030, USA
Integr Biol (Camb) 5:204-14. 2012..This demonstrates that actin kinetics can be monitored in a living cell by measuring force at the membrane, and used to probe the mobility of cells including cancer cells... - Functional expression and microdomain localization of prestin in cultured cellsAngela K Sturm
Bobby R Alford Department of Otolaryngology Head and Neck Surgery, Baylor College of Medicine, Houston, TX 77030, USA
Otolaryngol Head Neck Surg 136:434-9. 2007..A model system to study prestin employs its transfection into cultured HEK 293 cells. Our goal was to characterize prestin's trafficking pathway and localization in the plasma membrane... - Compartmentalization of the outer hair cell demonstrated by slow diffusion in the extracisternal spaceOlga Gliko
Department of Otorhinolaryngology Head and Neck Surgery, Baylor College of Medicine, Houston, Texas, USA
Biophys J 97:1215-24. 2009..Compared with free solution, the diffusion of 10 kDa dextran was slowed down in both the ECiS and the axial core by a factor of 4.6 and 1.6, respectively... - Voltage-dependent capacitance of human embryonic kidney cellsBrenda Farrell
Department of Otolaryngology and Head and Neck Surgery, Baylor College of Medicine, Houston, TX 77030, USA
Phys Rev E Stat Nonlin Soft Matter Phys 73:041930. 2006..We suggest that the capacitance-voltage relationships should be similar to that observed for HEK cells for OHCs that do not express prestin in their membranes... - Outer hair cell piezoelectricity: frequency response enhancement and resonance behaviorErik K Weitzel
Bobby R. Alford Department of Otorhinolaryngology and Communicative Sciences, Baylor College of Medicine, Houston, Texas 77030, USA
J Acoust Soc Am 114:1462-6. 2003..It is also possible that members of some mammalian orders use outer hair cell piezoelectric resonance in detecting species-specific vocalizations... - Identification of functionally important residues/domains in membrane proteins using an evolutionary approach coupled with systematic mutational analysisLavanya Rajagopalan
Bobby R Alford Department of Otolaryngology HNS, Baylor College of Medicine, Houston, TX, USA
Methods Mol Biol 493:287-97. 2009.... - Efferent modulation of hair cell functionRichard D Rabbitt
Department of Bioengineering, University of Utah, Salt Lake City, Utah, USA
Curr Opin Otolaryngol Head Neck Surg 19:376-81. 2011..This review covers the articles published between 2010 and early 2011 that presented new findings on inner-ear efferents and their ability to modulate hair cell function... - Membrane tether formation from outer hair cells with optical tweezersZhiwei Li
Department of Bioengineering, Rice University, Houston, Texas 77251, USA
Biophys J 82:1386-95. 2002..The information these studies provide on the mechanical properties of the OHC lateral wall is important for understanding the mechanism of OHC electromotility... - Urocortin-deficient mice display normal stress-induced anxiety behavior and autonomic control but an impaired acoustic startle responseXiaozhong Wang
Department of Molecular and Human Genetics, Division of Neuroscience, Baylor College of Medicine, Houston, Texas 77030, USA
Mol Cell Biol 22:6605-10. 2002..Ucn may modulate the acoustic startle response through the Ucn-expressing neuron projections from the region of the Edinger-Westphal nucleus... - Outer hair cell active force generation in the cochlear environmentZhijie Liao
Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland 21205, USA
J Acoust Soc Am 122:2215-25. 2007..Our findings can provide a better understanding of the outer hair cell's role in the cochlear amplifier... - Mechanosensitive channels in the lateral wall can enhance the cochlear outer hair cell frequency responseAlexander A Spector
Department of Biomedical Engineering, Johns Hopkins University, 720 Rutland Avenue, Traylor 411, Baltimore, MD 21205, USA
Ann Biomed Eng 33:991-1002. 2005..The effect of the channels is especially significant in an intermediate range of sound frequencies, and the channel-related gain is up to 3-4 times between 3 and 15 kHz... - Effects of prestin on membrane mechanics and electromechanicsBahman Anvari
Department of Bioengineering, University of California, Riverside, CA 92521, USA
Conf Proc IEEE Eng Med Biol Soc 2007:5384-6. 2007..We propose that prestin and membrane work in synergy to produce the electrical and mechanical changes that are required during OHC electromotility...
Research Grants
- MECHANICS OF THE COCHLEAR OUTER HAIR CELLWILLIAM BROWNELL; Fiscal Year: 2005..The model will allow us to predict force-deformation relationships for the OHC at acoustic frequencies and it will meet all the criteria for the mechanism of OHC force production. ..
- Electromechanics of the Cochlear Outer Hair CellWILLIAM BROWNELL; Fiscal Year: 2007..The final result will be a complete description of OHC electromechanical force production and its role in hearing. ..
- INTRACOCHLEAR ELECTROCHEMICAL GRADIENTSWILLIAM BROWNELL; Fiscal Year: 2007..Clarification of the physical principles underlying electromotility will also contribute to the emerging field of biological nanotechnology. ..
- Research Training in Otolaryngology Head & Neck SurgeryWILLIAM BROWNELL; Fiscal Year: 2007....
- INTRACOCHLEAR ELECTROCHEMICAL GRADIENTSWILLIAM BROWNELL; Fiscal Year: 2009..In addition to potential benefits to the hearing impaired, elucidation of the physical principles underlying the outer hair cell motor will contribute to the emerging field of biological nanotechnology. ..
- INTRACOCHLEAR ELECTROCHEMICAL GRADIENTSAlexander A Spector; Fiscal Year: 2010..In addition to potential benefits to the hearing impaired, elucidation of the physical principles underlying the outer hair cell motor will contribute to the emerging field of biological nanotechnology. ..
- MECHANICS OF THE COCHLEAR OUTER HAIR CELLWILLIAM BROWNELL; Fiscal Year: 2000..Knowledge of the microstructural elements and their functional significance in OHC mechanics will enhance our understanding of how the OHC contributes to mammalian hearing in health and disease. ..
- INTRACOCHLEAR ELECTROCHEMICAL GRADIENTSWILLIAM BROWNELL; Fiscal Year: 2002..Clarification of the physical principles underlying electromotility will also contribute to the emerging field of biological nanotechnology. ..
- Electromechanics of the Cochlear Outer Hair CellWilliam E Brownell; Fiscal Year: 2011..The final result will be a complete description of OHC electromechanical force production and its role in hearing. ..