Research Topics
| W WangSummaryAffiliation: Bayer HealthCare Country: USA Publications
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Detail Information
Publications
Dual effects of Tween 80 on protein stabilityWei Wang
Pfizer Inc, Global Biologics, 700 Chesterfield Parkway West, Chesterfield, MO 63017, USA
Int J Pharm 347:31-8. 2008..Addition of 0.1% Tween 80 not only increased the rate of oxidation in general but also altered the temperature-dependency of IL-2 mutein oxidation...- Coagulation factor VIII: structure and stabilityWei Wang
Analytics and Formulation, Biotechnology Division, Bayer HealthCare, 800 Dwight Way, Berkeley, CA 94701, USA
Int J Pharm 259:1-15. 2003.... - Peroxide formation in polysorbate 80 and protein stabilityEmily Ha
Analytics and Formulation Department, Process Sciences, Bayer Biotechnology, 800 Dwight Way, Berkeley, California 94701, USA
J Pharm Sci 91:2252-64. 2002.... - Correlation of rFVIII inactivation with aggregation in solutionWei Wang
Analytics and Formulation, Process Sciences, Biotechnology, Bayer Corp, 800 Dwight Way, Berkeley, California 94701, USA
Pharm Res 20:693-700. 2003..This study was designed to investigate the stability of recombinant FVIII (rFVIII) in solution at different pHs and to probe the cause(s) of rFVIII inactivation under accelerated storage conditions... - Antibody structure, instability, and formulationWei Wang
Pfizer, Inc, Global Biologics, 700 Chesterfield Parkway West, Chesterfield, Missouri 63017, USA
J Pharm Sci 96:1-26. 2007..Various aspects of formulation development have been examined to identify the critical attributes for the stabilization of antibodies... - Lyophilization and development of solid protein pharmaceuticalsW Wang
Biotechnology, Bayer Corporation, 800 Dwight Way, Berkeley, CA 94701, USA
Int J Pharm 203:1-60. 2000.... - A novel method for removing residual acetone from gelatin microspheresWei Wang
Biotechnology, Bayer Corporation, Berkeley, CA 94701, USA
Pharm Dev Technol 7:169-80. 2002..To develop a method for removing residual acetone from gelatin microspheres... - Oxidation of protein by vaporized sanitizing agentsWei Wang
Analytics and Formulation, Biotechnology Division, Bayer HealthCare, Berkeley, CA 94701, USA
PDA J Pharm Sci Technol 58:121-9. 2004..These results strongly suggest that the sanitization process needs to be properly controlled and closely monitored during manufacturing of drug products that are sensitive to oxidation... - Instability, stabilization, and formulation of liquid protein pharmaceuticalsW Wang
Biotechnology, Bayer Corporation, 800 Dwight Way, Berkeley, CA 94701, USA
Int J Pharm 185:129-88. 1999.... - Stabilization of a polypeptide in non-aqueous solventsWei Wang
Bayer HealthCare, 800 Dwight Way, Berkeley, CA 94701, United States
Int J Pharm 351:1-7. 2008..The stabilization approaches are applicable only in a non-aqueous environment for this peptide, and possibly for other peptides with similar reactive moieties... - pH dependent effect of glycosylation on protein stabilityWei Wang
Pfizer Inc, Global Biologics, 700 Chesterfield Parkway West, Chesterfield, MO 63017, USA
Eur J Pharm Sci 33:120-7. 2008..0 and both forms are most stable at pH 5.5 in terms of thermal stability, precipitation rate and total degradation rate. This study clearly demonstrates that the effect of glycosylation on the stability of a protein is pH-dependent... - [Peptide mapping analysis of recombinant human interleukin-11 with HPLC-ESI-Q-TOF/MS spectrometry]Ying Yang
National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China
Yao Xue Xue Bao 41:756-60. 2006..To analyze the peptide mapping of recombinant human interleukin-11 (rhIL-11) by HPLC-ESI-Q-TOF/MS spectrometry... - Evaluation of the in vitro and in vivo genotoxicity of magnolia bark extractNing Li
Department of Toxicological Safety Assessment, National Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing, China
Regul Toxicol Pharmacol 49:154-9. 2007..The results of these studies demonstrate that MBE is not genotoxic under the conditions of the in vitro bacterial reverse mutation assay and the in vivo micronucleus test, and support the safety of MBE for dietary consumption... - Role of glycosylation in corin zymogen activationXudong Liao
Department of Molecular Cardiology, Cleveland Clinic, Cleveland, Ohio 44195, USA
J Biol Chem 282:27728-35. 2007..Mutations altering the two N-glycosylation sites in the protease domain of rat corin prevented its activation in HEK 293 and HL-1 cells. Our results indicate that N-linked oligosaccharides play an important role in corin activation... - Grouping of amino acids and recognition of protein structurally conserved regions by reduced alphabets of amino acidsJing Li
National Laboratory of Solid State Microstructure and Department of Physics, Nanjing University, Nanjing, 210093, China
Sci China C Life Sci 50:392-402. 2007..The results indicate that the accuracy or efficiency of sequence alignment can be improved with the optimal reduced alphabet with N around 9... - In vivo culture of encapsulated endostatin-secreting Chinese hamster ovary cells for systemic tumor inhibitionYing Zhang
Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, People s Republic of China
Hum Gene Ther 18:474-81. 2007..4% and enhanced the survival of treated mice to 80% by 27 days posttreatment. Continuous systemic release of endostatin from microcapsules offers an effective therapeutic strategy to eradicate solid tumors... - [VNTR polymorphism of C6orf37 in Chinese population]Jing Cui
Department of Pathology, College of Medicine, Zhejiang University, Hangzhou 310031, China
Zhejiang Da Xue Xue Bao Yi Xue Ban 35:354-9. 2006..To identify a novel VNTR in C6orf37 and to detect the C6orf37 VNTR polymorphism distribution in Chinese population... - Self-assembly of nano-hydroxyapatite on multi-walled carbon nanotubesSusan Liao
Nanoscience and Nanotechnology Initiative, Division of Bioengineering, Faculty of Engineering, National University of Singapore, Singapore 117576, Singapore
Acta Biomater 3:669-75. 2007..Spindle-shaped units that are detached from the MWCNT template are able to maintain the ordered parallel structure of the nHA polycrystal fibril. We have thus created a self-assembled hydroxyapatite on MWCNTs... - Scanning tunneling microscopy study of DNA-chromophore motif on solid surfacesWenli Deng
College of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China
J Phys Chem B 111:6544-8. 2007.... - Optimization of microencapsulated recombinant CHO cell growth, endostatin production, and stability of microcapsule in vivoYing Zhang
Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese, Academy of Sciences, Dalian 116023, People s Republic of China
J Biomed Mater Res B Appl Biomater 84:79-88. 2008..The optimized microencapsulation conditions for in vivo implantation are liquid core and 240 microm in diameter. This study provides useful information for antiangiogenic gene therapy to tumors using microencapsulated recombinant cells... - Combinatorial protein design strategies using computational methodsHidetoshi Kono
Computational Biology Group, Neutron Science Research Center, Quantum Beam Science Directorate, Japan Atomic Energy Agency, Kyoto, Japan
Methods Mol Biol 352:3-22. 2007..Such methods may be used to guide the construction of both individual sequences and combinatorial libraries of proteins... - The selective retention of lutein, meso-zeaxanthin and zeaxanthin in the retina of chicks fed a xanthophyll-free dietYingMing Wang
Division of Endocrinology, Diabetes and Clinical Nutrition, Department of Medicine, L465, Oregon Health and Science University, Portland, OR 972391, USA
Exp Eye Res 84:591-8. 2007..It is clear that these xanthophylls may have an essential role in the cone-rich retina of the chick as evidenced by their selective retention... - Characterization of active phenolic components in the ethanolic extract of Ananas comosus L. leaves using high-performance liquid chromatography with diode array detection and tandem mass spectrometryChao Ma
Laboratory of Pharmaceutical Sciences, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China
J Chromatogr A 1165:39-44. 2007..Meanwhile, a new HPLC-DAD-MS method was established for the identification and characterization of phenylpropane diglycerides in natural plants... - Acute electrical stimulation of nucleus ambiguus enhances immune function in ratsYing Wu Mei
College of Animal Science and Veterinary Medicine, Jilin University, Changchun, China
Can J Neurol Sci 35:441-7. 2008.... 
Redox modulation of long-term potentiation in the hippocampus via regulation of the glycogen synthase kinase-3beta pathwayFei Cai
Department of Pharmacology, Tongji Medical College, Wuhan, Hubei 430030, China
Free Radic Biol Med 45:964-70. 2008..These findings will benefit the understanding of the redox contribution to the mechanisms underlying synaptic plasticity and AD pathogenesis...- All-atom replica exchange molecular simulation of protein BBLJian Zhang
Department of Physics, National Laboratory of Solid State Microstructure, Institute of Biophysics, Nanjing University, Nanjing, China
Proteins 72:1038-47. 2008..Compared with the experimental data (Sadqi et al., Nature 2006; 442:317), our results indicate that the Naf-BBL (pH5.3) may have an even lower barrier height and cooperativity... - Discovery of azetidinone acids as conformationally-constrained dual PPARalpha/gamma agonistsWei Wang
Metabolic Diseases Chemistry, Bristol Myers Squibb Research and Development, Princeton, NJ 08543 5400, USA
Bioorg Med Chem Lett 18:1939-44. 2008..Synthesis, in vitro and in vivo activities of compounds in this series are described. A high-yielding method for N-arylation of azetidinone esters is also described... - Influence of pH on the aggregation morphology of a novel surfactant with single hydrocarbon chain and multi-amine headgroupsWei Wang
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Colloid and Interface Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, People s Republic of China
J Phys Chem B 112:1409-13. 2008.... - Identifying folding nucleus based on residue contact networks of proteinsJie Li
National Laboratory of Solid State Microstructure, Department of Physics, Nanjing University, Nanjing 210093, China
Proteins 71:1899-907. 2008..Our study shows that the FNRs can be identified solely from the native structures of proteins based on the analysis of residue contact network without any knowledge of the transition state ensemble... - Crystal structure of long-chain alkane monooxygenase (LadA) in complex with coenzyme FMN: unveiling the long-chain alkane hydroxylaseLiu Li
Tsinghua Nankai IBP Joint Research Group for Structural Biology, Tsinghua University, Beijing 100084, China
J Mol Biol 376:453-65. 2008..The LadA structure opens a rational perspective to explore and alter the substrate binding site of LadA, with potential biotechnological applications in areas such as petroleum exploration and treatment of environmental oil pollution... - HMGA1a protein unfolds or refolds synthetic DNA-chromophore hybrid polymers: a chaperone-like behaviorWei Wan
Department of Chemistry, Washington State University, Pullman, WA 99164, USA
Chembiochem 9:304-11. 2008..The unfolding mechanisms and the binding stoichiometry of protein-hybrid polymers depended on the sequence of the synthetic polymers... 
On the detection of functionally coherent groups of protein domains with an extension to protein annotationWilliam A McLaughlin
Department of Chemistry and Biochemistry, Center for Theoretical Biological Physics, University of California, San Diego, 9500 Gilman Drive La Jolla, CA 92093 0359, USA
BMC Bioinformatics 8:390. 2007..Further effort is needed to identify groups of domains that span across two or more proteins and are linked by a cooperative function. Such functional domain combinations can be useful for protein annotation...- Effects of solution conditions, processing parameters, and container materials on aggregation of a monoclonal antibody during freeze-thawingLisa A Kueltzo
Center for Pharmaceutical Biotechnology, Department of Pharmaceutical Sciences, University of Colorado Health Sciences Center, 4200 East 9th Avenue, Denver, Colorado 80262, USA
J Pharm Sci 97:1801-12. 2008..Samples stressed in plastic or glass containers contained low amounts of aggregate. Storage in Teflon or commercial freezing containers, however, led to significantly higher levels of aggregate formation... - Triptolide alters mitochondrial functionsYing Su
Department of Radiation Oncology, University of Rochester Medical Center, Rochester, NY 14642, USA
Adv Exp Med Biol 599:139-46. 2007..It is likely that by impairment of mitochondrial function, TPL exerts its inhibitory effect on growth of tumor and progression of inflammatory disease... - Proliferation, viability, and metabolism of human tumor and normal cells cultured in microcapsuleXulang Zhang
Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China
Appl Biochem Biotechnol 134:61-76. 2006..In addition, it lays a solid foundation for the use of microencapsulation in cell culture in vitro and cell implantation in vivo... - Conditional gene modification in mouse liver using hydrodynamic delivery of plasmid DNA encoding Cre recombinaseHuan Zhang Zhu
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, China
FEBS Lett 580:4346-52. 2006..Thus, hydrodynamic gene delivery of the Cre recombinase provides a valuable approach for Cre-loxP-mediated conditional gene modification in the liver of mice... - Adsorption mechanism at the molecular level between polymers and uremic octapeptide by the 2D 1H NMR TechniqueGuohua Li
The Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, Nankai University, Tianjin 300071, People's Republic of China
Biomacromolecules 7:1811-8. 2006.... - [Human peripheral blood CD4+ T-lymphocytes reconstituting severe combined immunodeficient mouse-human cell immune system and its immunological function]Qiu-hua Zeng
Department of Radiology, Third Xiangya Hospital, Central South University, Chaengsha 410013, China
Zhong Nan Da Xue Xue Bao Yi Xue Ban 29:513-6. 2004..CONCLUSION: Intraperitoneal injection of SCID mice with mature human peripheral blood CD4+ T lymphocytes can result in a human cell immune system. The method is simple, quick and has abundant donors... - Development of an in vitro multicellular tumor spheroid model using microencapsulation and its application in anticancer drug screening and testingXulang Zhang
Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China
Biotechnol Prog 21:1289-96. 2005..It demonstrated that the MMTS has the potential to be a rapid and valid in vitro model to screen chemotherapeutic drugs with a feature to mimic in vivo three-dimensional (3-D) cell growth pattern... - Separation and determination of flavonoids in Agrimonia pilosa Ledeb. by capillary electrophoresis with electrochemical detectionXueqin Xu
Department of Chemistry, Fuzhou University, Fuzhou, Fujian, 350002, China
J Sep Sci 28:647-52. 2005..02 to 0.05 microg/mL for all compounds. This method was successfully used to determine the above five flavonoids in Agrimonia pilosa Ledeb. with relatively simple extraction procedures, and the assay results were satisfactory... - Development and evaluation of an enzyme-linked immunosorbent assay for detection of antibodies against the spike protein of SARS-coronavirusJincun Zhao
Department of Immunology, Peking University Health Science Center, 38 Xueyuan Road, Beijing 100083, PR China
J Clin Virol 33:12-8. 2005..None of the serum samples from 100 healthy blood donors was positive in the S450-650-based assay. CONCLUSION: The S450-650-based ELISA can detect anti-S IgG Abs with high sensitivity and specificity... - High performance liquid chromatographic-mass spectrometric determination of ginsenoside Rg3 and its metabolites in rat plasma using solid-phase extraction for pharmacokinetic studiesHai-Tang Xie
Key Laboratory of Drug Metabolism and Pharmacokinetics, 24 Tong Jia Xiang Street, Mail Box 210, China Pharmaceutical University, Nanjing 210009, Jiangsu Province, China
J Chromatogr B Analyt Technol Biomed Life Sci 818:167-73. 2005..1, 0.02 and 0.08 ng in each 10 microl injection onto the HPLC column) for Rg3, Rh2 and ppd, respectively. The method has been demonstrated to be highly sensitive and accurate for the determination of Rg3 and its metabolites in rat plasma... - Protein aggregation and its inhibition in biopharmaceuticsWei Wang
Biotechnology Division, Bayer HealthCare, 800 Dwight Way, Berkeley, CA 94701, USA
Int J Pharm 289:1-30. 2005.... - [Determination of entrapment efficiency of breviscapine nanoliposomes]Fei Xiong
Institute of Pharmaceutics, China Pharmaceutical University, Nanjing 210009, China
Yao Xue Xue Bao 39:755-7. 2004..8)%. The entrapment efficiency of breviscapine nanoliposomes was (75.3 +/- 2.3)%. CONCLUSION: The method used in this study is simple, applicable and accurate for determination of the entrapment efficiency of breviscapine nanoliposomes... - Molecular and biochemical characterization of the Skp2-Cks1 binding interfaceWei Wang
Department of Chemistry and Biochemistry, University of Colorado Boulder, Boulder, Colorado 80309, USA
J Biol Chem 279:51362-9. 2004..These results uncovered the specificity of the Skp2-Cks1 interaction and reveal a critical function for the structurally flexible carboxyl-terminal tail region of Skp2 in Cks1 recognition and substrate ubiquitination... - Thermodynamic stability and kinetic foldability of a lattice protein modelJie Li
National Lab of Solid State Microstructure and Physics Department, Nanjing University, Nanjing 210093, China
J Chem Phys 120:6274-87. 2004..The folding of model proteins with the MMJ potentials is fast. However, the relationship between the foldability and the thermodynamic stability of the mutated sequences is complex... - The number of protein folds and their distribution over families in natureXinsheng Liu
National Lab of Solid State Microstructure, Department of Physics and Institute of Biophysics, Nanjing University, Nanjing, China
Proteins 54:491-9. 2004..In addition, the method of directly "enlarging" the sample to the population may be useful in inferring distributions of species in different fields... - Computational design and characterization of a monomeric helical dinuclear metalloproteinJennifer R Calhoun
Department of Biochemistry and Molecular Biophysics, Johnson Foundation, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
J Mol Biol 334:1101-15. 2003..DFsc is then a realization of complete de novo design, where backbone structure, activity, and sequence are specified in the design process... - Autophosphorylation of the catalytic subunit of the DNA-dependent protein kinase is required for efficient end processing during DNA double-strand break repairQi Ding
College of Veterinary Medicine and Department of Pathobiology and Diagnostic Investigation, Michigan State University, East Lansing, Michigan 48824, USA
Mol Cell Biol 23:5836-48. 2003..However, autophosphorylation at these sites may not be sufficient for kinase disassembly... - Folding behavior of chaperonin-mediated substrate proteinWei-Xin Xu
National Laboratory of Solid State Microstructure, Department of Physics, Nanjing University, Nanjing, China
Proteins 61:777-94. 2005.... - Determination of schizandrin in rat plasma by high-performance liquid chromatography-mass spectrometry and its application in rat pharmacokinetic studiesMeijuan Xu
Key Lab of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210009, China
J Chromatogr B Analyt Technol Biomed Life Sci 828:55-61. 2005..005, 0.100 and 1.000 microg/mL. Intra- and inter-day relative standard deviations were 5.95-12.93% and 3.87-14.53%, respectively. This method was successfully applied for the pharmacokinetic studies in rats... - Structure-based function inference using protein family-specific fingerprintsDeepak Bandyopadhyay
Department of Computer Science, University of North Carolina at Chapel Hill, North Carolina 27599, USA
Protein Sci 15:1537-43. 2006..Some predictions have been corroborated by other computational methods and some validated by subsequent functional characterization... - Preparation of spherical iron nanoclusters in ethanol-water solution for nitrate removalWei Wang
College of Environment Science and Engineering, Nankai University, Nankai District, Tianjin, PR China
Chemosphere 65:1396-404. 2006..Finally, kinetic analysis revealed that chemical reduction of nitrate by HNZVI could not be described by the first- or pseudo-first-order kinetic model... - Reversal of the malignant phenotype of cervical cancer CaSki cells through adeno-associated virus-mediated delivery of HPV16 E7 antisense RNASufang Wu
Cancer Biology Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, Republic of China
Clin Cancer Res 12:2032-7. 2006.... - Carbon nanotubes for immunomagnetic separation of Escherichia coli O157: H7Yi Lin
Department of Chemistry, Laboratory for Emerging Materials and Technology, Clemson University, Clemson, SC 29634-097, USA
J Nanosci Nanotechnol 6:868-71. 2006.... - Identification of a novel VNTR polymorphism in C6orf37 and its association with colorectal cancer risk in Chinese populationJing Cui
Department of Pathology, School of Medicine, Zhejiang University, Yan An Road 353, Hangzhou, 310031, China
Clin Chim Acta 368:155-9. 2006..C6orf37 was a gene up-regulated in colorectal adenoma in our previous study. A variable region of C6orf37 sequence was found when we blasted its full sequence with NCBI nucleotide database... - Freeze-drying of liposomes using tertiary butyl alcohol/water cosolvent systemsJingXia Cui
School of Pharmacy, Hebei Medical University, No 361, Zhongshan East Road, Shijiazhuang City, Hebei Province 050017, PR China
Int J Pharm 312:131-6. 2006..In addition, in conjunction with a modified injection method, this FD technology might be used to produce dehydrated HSPC liposomes on a large scale... - Folding with downhill behavior and low cooperativity of proteinsGuanghong Zuo
Department of Physics, Nanjing University, China
Proteins 63:165-73. 2006..These could enable us to identify the downhill folding or the cooperative two-state folding behavior solely from the native structures of proteins... - New surface-enhanced Raman spectroscopy substrates via self-assembly of silver nanoparticles for perchlorate detection in waterWei Wang
Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA
Appl Spectrosc 59:1509-15. 2005..These capillary flow cells could be readily configured to allow for in situ, nondestructive detection of ClO4- via fiber optics... - Exploiting sequence and structure homologs to identify protein-protein binding sitesJo-Lan Chung
Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla 92093-0537, USA
Proteins 62:630-40. 2006..The results support the hypothesis that in many cases protein interfaces require some residues to provide rigidity to minimize the entropic cost upon complex formation... - Identification and determination of major flavonoids in rat urine by HPLC-UV and HPLC-MS methods following oral administration of Dalbergia odorifera extractRongxia Liu
School of Pharmaceutical Sciences, Peking University, Beijing 100083, People's Republic of China
Biomed Chromatogr 20:101-8. 2006..odorifera extract... - The use of LC/MS, GC/MS, and LC/NMR hyphenated techniques to identify a drug degradation product in pharmaceutical developmentChangkang Pan
Pharmaceutical and Analytical Development, Novartis Pharmaceuticals Corporation, One Health Plaza, East Hanover, NJ 07936, USA
J Pharm Biomed Anal 40:581-90. 2006..The combination approach is critical for unambiguous impurity structure elucidation in drug degradation studies of pharmaceutical drug products... - Folding behaviors of lattice model proteins with three kinds of contact potentialsMeng Qin
National Laboratory of Solid State Microstructure and Department of Physics, Nanjing University, China
Phys Rev E Stat Nonlin Soft Matter Phys 67:061905. 2003..The formations of various contacts are also analyzed, which provide us with some microscopic information on the model systems and interaction potentials... - Identification and characterization of AGTRAP, a human homolog of murine Angiotensin II Receptor-Associated Protein (Agtrap)Wei Wang
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, People s Republic of China
Int J Biochem Cell Biol 34:93-102. 2002..We suggest that the AGTRAP-RACK1 interaction may help to recruit signaling complex to the AT(1) receptor to affect AT(1) receptor signaling...