Research Topics
Genomes and GenesSpecies | M C GomezSummaryAffiliation: Audubon Nature Institute Center for Research of Endangered Species Country: USA Publications
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Detail Information
Publications
151 evaluation of reference gene transcript stability during early pre-implantation development and in spermatogonial cells in the catM N Biancardi
Audubon Center for Research of Endangered Species, New Orleans, LA, USA
Reprod Fertil Dev 24:187-8. 2011....
Scriptaid and 5-aza-2'deoxycytidine enhanced expression of pluripotent genes and in vitro developmental competence in interspecies black-footed cat cloned embryosM C Gomez
Audubon Center for Research of Endangered Species, New Orleans, LA 70131, USA
Reprod Domest Anim 47:130-5. 2012....
Generation of domestic transgenic cloned kittens using lentivirus vectorsMartha C Gómez
Audubon Center for Research of Endangered Species, New Orleans, Louisiana 70131, USA
Cloning Stem Cells 11:167-76. 2009..In summary, we report the birth of transgenic cloned kittens produced by LV vector-mediated transduction of donor cells and confirm that cloned kittens express the EGFP reporter transgene in all body tissues...
Nuclear transfer of sand cat cells into enucleated domestic cat oocytes is affected by cryopreservation of donor cellsMartha C Gómez
Audubon Center for Research of Endangered Species, New Orleans, Louisiana 70131, USA
Cloning Stem Cells 10:469-83. 2008..Consequently, the number of embryos that cleaved, implanted, and developed to term-gestation and POU5F1 expression in derived blastocysts indirectly was affected...
Derivation of cat embryonic stem-like cells from in vitro-produced blastocysts on homologous and heterologous feeder cellsM C Gomez
Audubon Center for Research of Endangered Species, New Orleans, LA, USA
Theriogenology 74:498-515. 2010..During culture, cESL colonies spontaneously differentiated into fibroblasts, cardiomyocytes, and embryoid bodies. Development of techniques to prevent differentiation of cESL cells will be essential for maintaining defined cell lines...
Chromosomal aneuploidy in African Wildcat somatic cells and cloned embryosMartha C Gómez
Audubon Center for Research of Endangered Species, 14001 River Road, New Orleans, LA 70131, USA
Cloning Stem Cells 8:69-78. 2006....
Cloning endangered felids using heterospecific donor oocytes and interspecies embryo transferMartha C Gómez
Audubon Center for Research of Endangered Species, 14001 River Road, New Orleans, LA 70124, USA
Reprod Fertil Dev 21:76-82. 2009..In addition, we discuss epigenetic events that take place in donor cells and felid cloned embryos and mtDNA inheritance in wild felid clones and their offspring...
Nuclear transfer in cats and its applicationM C Gomez
Audubon Nature Institute Center for Research of Endangered Species, New Orleans, LA 70131, USA
Theriogenology 66:72-81. 2006..It is important to continue evaluating these animals throughout their lives and to examine their capability for natural reproduction...
Trichostatin A modified histone covalent pattern and enhanced expression of pluripotent genes in interspecies black-footed cat cloned embryos but did not improve in vitro and in vivo viabilityMartha C Gómez
Audubon Center for Research of Endangered Species, New Orleans, Louisiana, USA
Cell Reprogram 13:315-29. 2011..However, genomic reprogramming of Is-BFC cloned blastocysts did not follow the same reprogramming pattern observed in DSH-IVF embryos, and in vitro and in vivo developmental competence was not enhanced...
Birth of African Wildcat cloned kittens born from domestic catsMartha C Gómez
Audubon Center for Research of Endangered Species, New Orleans, LA 70131, USA
Cloning Stem Cells 6:247-58. 2004..Subsequent DNA analysis of 12 cat-specific microsatellite loci confirmed that all 17 kittens were clones of the AWC donor male. These AWC kittens represent the first wild carnivores to be produced by nuclear transfer...
Nuclear transfer of synchronized african wild cat somatic cells into enucleated domestic cat oocytesMartha C Gómez
Department of Animal Science, Louisiana State University, Baton Rouge, Louisiana 70808, USA
Biol Reprod 69:1032-41. 2003..Incomplete reprogramming of the differentiated nucleus may be a major constraint to the in vivo developmental potential of the embryos...
Births of kittens produced by intracytoplasmic sperm injection of domestic cat oocytes matured in vitroM C Gomez
The Audubon Institute Center for Research of Endangered Species, New Orleans, LA 70131, USA
Reprod Fertil Dev 12:423-33. 2000....
In vitro production and transfer of cat embryos in the 21st centuryC E Pope
Audubon Nature Institute Center for Research of Endangered Species, New Orleans, LA 70131, USA
Theriogenology 66:59-71. 2006..Furthermore, extrapolating the domestic biotechniques to non-domestic felids has produced encouraging results in some species...
Birth of domestic cat kittens of predetermined sex after transfer of embryos produced by in vitro fertilization of oocytes with flow-sorted spermC E Pope
Audubon Center for Research of Endangered Species, 14001 River Road, New Orleans, LA 70131, USA
Theriogenology 71:864-71. 2009..In conclusion, we confirmed that sperm sorting technology can be applied to domestic cats and established that kittens of predetermined sex can be produced...
286 expression of pluripotent stem cell markers in domestic cat spermatogonial cellsR H Powell
University of New Orleans, New Orleans, LA
Reprod Fertil Dev 25:290-1. 2012....
In vivo survival of domestic cat oocytes after vitrification, intracytoplasmic sperm injection and embryo transferC E Pope
Audubon Center for Research of Endangered Species, New Orleans, Louisiana, USA
Theriogenology 77:531-8. 2012..On Days 63 and 66 of gestation, four live kittens were born. In vivo viability of zygotes and/or embryos produced via ICSI of vitrified oocytes was established by birth of live kittens after transfer to recipients...
Isolation, culture and characterisation of somatic cells derived from semen and milk of endangered sheep and eland antelopeL Nel Themaat
Department of Animal Sciences, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA
Reprod Fertil Dev 19:576-84. 2007..In conclusion, we have developed a technique for obtaining somatic cells from semen. We have also demonstrated that semen-derived cells can serve as karyoplast donors for nuclear transfer...
In vitro embryo production and embryo transfer in domestic and non-domestic catsC E Pope
Audubon Nature Institute Center for Research of Endangered Species, New Orleans, LA 70131, USA
Theriogenology 66:1518-24. 2006..9, 23.5, 22.0, 23.1, 23.5 and 40.9 oocytes (P > 0.05) were recovered at the first through the sixth treatment cycles from 138, 138, 97, 49, 22, and seven retrievals, respectively...
219 isolation and characterization of domestic cat spermatogonial cellsR H Powell
University of New Orleans, New Orleans, LA, USA
Reprod Fertil Dev 24:221-2. 2011....
64 cryopreservation of cat ovarian tissue by vitrificationJ Galiguis
Audubon Center for Research of Endangered Species, New Orleans, LA, USA
Reprod Fertil Dev 25:179. 2012..Despite this, tolerance of such follicles to cryopreservation was improved by vitrifying and warming in cryo-media containing 10nM glutathione...
54 cryopreservation of domestic cat epididymal sperm in a defined extender without animal or plant proteinsJ R Saenz
Louisiana State University Agricultural Center, Baton Rouge, LA, USA
Reprod Fertil Dev 24:139. 2011..Furthermore, we confirmed that addition of cryoprotectant (i.e. glycerol) after gradual cooling to 4°C is beneficial to post-thaw survival...
85 domestic cat kittens born after transfer of cryopreserved embryos produced by in vitro fertilization of oocytes with flow-sorted spermC E Pope
Audubon Center for Research of Endangered Species, New Orleans, LA, USA
Reprod Fertil Dev 23:148. 2011....
Applying embryo cryopreservation technologies to the production of domestic and black-footed catsC E Pope
Audubon Center for Research of Endangered Species, New Orleans, LA 70131, USA
Reprod Domest Anim 47:125-9. 2012....
122 birth of black-footed cat kittens after transfer of cryopreserved embryos produced by in vitro fertilization of oocytes with cryopreserved spermC E Pope
Audubon Nature Institute Center for Research of Endangered Species, New Orleans, LA, USA
Reprod Fertil Dev 24:173. 2011..In summary, we have further demonstrated that assisted reproductive technology can be used for conservation of rare and vulnerable small felids...
