Maria Erali

Summary

Affiliation: ARUP Institute for Clinical and Experimental Pathology
Country: USA

Publications

  1. pmc Human papillomavirus genotyping using an automated film-based chip array
    Maria Erali
    ARUP Laboratories, 500 Chipeta Way, Salt Lake City, UT 84108, USA
    J Mol Diagn 11:439-45. 2009
  2. pmc High resolution melting applications for clinical laboratory medicine
    Maria Erali
    ARUP Institute for Clinical and Experimental Pathology, University of Utah, Salt Lake City, UT 84108, USA
    Exp Mol Pathol 85:50-8. 2008
  3. doi request reprint SNP genotyping by unlabeled probe melting analysis
    Maria Erali
    Advanced Technology Group, Institute for Clinical and Experimental Pathology, ARUP Laboratories, Salt Lake City, UT, USA
    Methods Mol Biol 429:199-206. 2008
  4. ncbi request reprint Closed-tube SNP genotyping without labeled probes/a comparison between unlabeled probe and amplicon melting
    Michael Liew
    Institute for Clinical and Experimental Pathology, ARUP, Salt Lake City, UT 84108 1221, USA
    Am J Clin Pathol 127:341-8. 2007
  5. ncbi request reprint Localization and imaging of nucleic acids on nanoporous aluminum oxide membranes
    Maria Erali
    ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT 84108, USA
    Clin Chem 50:1819-21. 2004
  6. pmc High resolution melting analysis for gene scanning
    Maria Erali
    ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT 84108, USA
    Methods 50:250-61. 2010
  7. pmc Genotyping of human platelet antigens 1 to 6 and 15 by high-resolution amplicon melting and conventional hybridization probes
    Michael Liew
    ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108 1221, USA
    J Mol Diagn 8:97-104. 2006
  8. ncbi request reprint Increased sensitivity of bacterial detection in cerebrospinal fluid by fluorescent staining on low-fluorescence membrane filters
    Jacob D Durtschi
    ARUP Institute for Clinical and Experimental Pathology and Department of Pathology, University of Utah, Salt Lake City, UT 84108, USA
    J Med Microbiol 54:843-50. 2005
  9. ncbi request reprint Genotyping of single-nucleotide polymorphisms by high-resolution melting of small amplicons
    Michael Liew
    Institute for Clinical and Experimental Pathology, ARUP, Salt Lake City, UT, USA
    Clin Chem 50:1156-64. 2004
  10. ncbi request reprint Multiplex genotyping by melting analysis of loci-spanning probes: beta-globin as an example
    Genevieve Pont-Kingdon
    ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT 84108, USA
    Biotechniques 42:193-7. 2007

Detail Information

Publications20

  1. pmc Human papillomavirus genotyping using an automated film-based chip array
    Maria Erali
    ARUP Laboratories, 500 Chipeta Way, Salt Lake City, UT 84108, USA
    J Mol Diagn 11:439-45. 2009
    ..Based on its analytical performance, future studies with this platform are warranted to assess its clinical utility for HPV detection and genotyping...
  2. pmc High resolution melting applications for clinical laboratory medicine
    Maria Erali
    ARUP Institute for Clinical and Experimental Pathology, University of Utah, Salt Lake City, UT 84108, USA
    Exp Mol Pathol 85:50-8. 2008
    ..In the clinical laboratory, it is an ideal format for in-house testing, with minimal cost and time requirements for new assay development...
  3. doi request reprint SNP genotyping by unlabeled probe melting analysis
    Maria Erali
    Advanced Technology Group, Institute for Clinical and Experimental Pathology, ARUP Laboratories, Salt Lake City, UT, USA
    Methods Mol Biol 429:199-206. 2008
    ..No real-time PCR or allele-specific amplification is needed. Unlabeled probes are inexpensive, provide the sequence specificity of probes, and allow simultaneous identification of multiple alleles by melting analysis...
  4. ncbi request reprint Closed-tube SNP genotyping without labeled probes/a comparison between unlabeled probe and amplicon melting
    Michael Liew
    Institute for Clinical and Experimental Pathology, ARUP, Salt Lake City, UT 84108 1221, USA
    Am J Clin Pathol 127:341-8. 2007
    ..When 2 temperature standards were used to bracket the amplicon of interest, the accuracy of amplicon genotyping of SNPs was increased to 100% (LightTyper) and 88% (I-Cycler)...
  5. ncbi request reprint Localization and imaging of nucleic acids on nanoporous aluminum oxide membranes
    Maria Erali
    ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT 84108, USA
    Clin Chem 50:1819-21. 2004
  6. pmc High resolution melting analysis for gene scanning
    Maria Erali
    ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT 84108, USA
    Methods 50:250-61. 2010
    ..For each step in the protocol, a general overview of principles is provided, followed by an in depth analysis of one example, scanning of CYBB, the gene that is mutated in X-linked chronic granulomatous disease...
  7. pmc Genotyping of human platelet antigens 1 to 6 and 15 by high-resolution amplicon melting and conventional hybridization probes
    Michael Liew
    ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108 1221, USA
    J Mol Diagn 8:97-104. 2006
    ..Advantages of high-resolution melting include rapid assay development and execution, no need for modified oligonucleotides, and similar accuracy in genotyping compared with other closed-tube melting methods...
  8. ncbi request reprint Increased sensitivity of bacterial detection in cerebrospinal fluid by fluorescent staining on low-fluorescence membrane filters
    Jacob D Durtschi
    ARUP Institute for Clinical and Experimental Pathology and Department of Pathology, University of Utah, Salt Lake City, UT 84108, USA
    J Med Microbiol 54:843-50. 2005
    ..5 order of magnitude increase in the bacterial concentration limit of detection...
  9. ncbi request reprint Genotyping of single-nucleotide polymorphisms by high-resolution melting of small amplicons
    Michael Liew
    Institute for Clinical and Experimental Pathology, ARUP, Salt Lake City, UT, USA
    Clin Chem 50:1156-64. 2004
    ..We adapted this system to genotype single-nucleotide polymorphisms (SNPs) after rapid-cycle PCR (12 min) of small amplicons (</=50 bp)...
  10. ncbi request reprint Multiplex genotyping by melting analysis of loci-spanning probes: beta-globin as an example
    Genevieve Pont-Kingdon
    ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT 84108, USA
    Biotechniques 42:193-7. 2007
    ..This demonstrates that, in addition to their haplotyping capabilities, LSProbes are able to genotype in a single step, loci 58 nucleotides apart...
  11. pmc Genotyping hepatitis C virus by heteroduplex mobility analysis using temperature gradient capillary electrophoresis
    Rebecca L Margraf
    ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, Utah 84108, USA
    J Clin Microbiol 42:4545-51. 2004
    ..Compared to conventional HMA, TGCE improves the resolution, with better separation of heteroduplexes and homoduplexes. All common HCV genotypes can be detected and differentiated by this HMA-TGCE assay...
  12. pmc A single-tube nucleic acid extraction, amplification, and detection method using aluminum oxide
    Shale Dames
    ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108, USA
    J Mol Diagn 8:16-21. 2006
    ..The AOM single tube method is a fast, reliable, and reproducible technique for the extraction, amplification, and detection of HSV in CSF...
  13. ncbi request reprint HLA-B27 typing: evaluation of an allele-specific PCR melting assay and two flow cytometric antigen assays
    Michael T Seipp
    ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, Utah, USA
    Cytometry B Clin Cytom 63:10-5. 2005
    ..Testing for HLA-B27 is of diagnostic value because 90% of patients with ankylosing spondylitis have the B27 antigen. Two commonly used HLA-B27 flow cytometric assays are commercially available...
  14. ncbi request reprint Single-tube method for nucleic acid extraction, amplification, purification, and sequencing
    Rebecca L Margraf
    ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT 84108, USA
    Clin Chem 50:1755-61. 2004
    ..We used a commercially available nucleic acid extraction system to develop a single-tube extraction-to-sequencing (STETS) method for HCV genotyping...
  15. pmc Performance characteristics of the COBAS Amplicor Hepatitis C Virus (HCV) Monitor, Version 2.0, International Unit assay and the National Genetics Institute HCV Superquant assay
    Eric Q Konnick
    ARUP Institute for Clinical and Experimental Pathology, University of Utah Health Sciences Center, Salt Lake City, Utah 84108, USA
    J Clin Microbiol 40:768-73. 2002
    ..Overall, the Amplicor and Superquant assays agree well, and results obtained in one assay could be expected to compare well with results from the other when reported in copies per milliliter...
  16. pmc Evaluation of the COBAS amplicor HBV monitor assay and comparison with the ultrasensitive HBV hybrid capture 2 assay for quantification of hepatitis B virus DNA
    Eric Q Konnick
    ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108, USA
    J Clin Microbiol 43:596-603. 2005
    ..Amplicor is linear from 200 to 200,000 HBV DNA copies/ml with undiluted samples, and this range can be expanded through dilution. Inconsistent HC2 results near the limit of detection justify use of a grey zone...
  17. ncbi request reprint Extraction and amplification of genomic DNA from human blood on nanoporous aluminum oxide membranes
    Marc G Elgort
    ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT 84108, USA
    Clin Chem 50:1817-9. 2004
  18. pmc Hepatitis C genotyping by denaturing high-performance liquid chromatography
    Michael Liew
    Institute for Clinical and Experimental Pathology, ARUP Laboratories, Salt Lake City, Utah 84108 122, USA
    J Clin Microbiol 42:158-63. 2004
    ..However, the dHPLC method was not as accurate as other reported methods of HCV genotyping. This is the first time that HCV genotyping has been performed by dHPLC...
  19. ncbi request reprint Evaluation of electronic microarrays for genotyping factor V, factor II, and MTHFR
    Maria Erali
    ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108, USA
    Clin Chem 49:732-9. 2003
    ..We evaluated a method using electronically addressable microarrays for the detection of mutations in these genes that have been associated with vascular disease...
  20. ncbi request reprint Multiplex single-color PCR with amplicon melting analysis for identification of Aspergillus species
    Maria Erali
    Clin Chem 52:1443-5. 2006