Research Topics
| John C H SpenceSummaryAffiliation: Arizona State University Country: USA Publications
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Detail Information
Publications
Diffraction and imaging from a beam of laser-aligned proteins: resolution limitsJ C H Spence
Department of Physics and Astronomy, Arizona State University, Tempe, AZ 85287 1504, USA
Acta Crystallogr A 61:237-45. 2005..For a typical virus (TMV), a 50 W continuous-wave laser is adequate for subnanometre resolution at room temperature. The dependence of resolution on laser power, temperature, molecular size, shape and dielectric constant is analyzed...
Lawrence Bragg, microdiffraction and X-ray lasersJ C H Spence
Department of Physics, Arizona State University, Tempe, 85282, USA
Acta Crystallogr A 69:25-33. 2013..In this way, using short pulses instead of freezing, the nexus between dose, resolution and crystal size has been broken, opening the way to time-resolved diffraction without damage for a stream of identical particles...- X-ray lasers for structural and dynamic biologyJ C H Spence
Department of Physics, Arizona State University, Tempe, AZ 85287, USA
Rep Prog Phys 75:102601. 2012..Possibilities for time-resolved chemistry using fast WAXS (solution scattering) from mixtures is reviewed, toward the general goal of making molecular movies of biochemical processes... - Phasing of coherent femtosecond X-ray diffraction from size-varying nanocrystalsJohn C H Spence
Department of Physics, Arizona State University, Tempe, AZ 85287, USA
Opt Express 19:2866-73. 2011..By using information on the particle-size distribution within the patterns, this digital method succeeds, using all the data, without knowledge of the distribution of particle size or requiring atomic-resolution data... - Three-dimensional diffractive imaging for crystalline monolayers with one-dimensional compact supportJ C H Spence
Department of Physics, Arizona State University, Tempe, AZ 85287 1504, USA
J Struct Biol 144:209-18. 2003..The method should greatly reduce the time and labor needed for data acquisition and analysis in cryo-electron microscopy of organic thin crystals by avoiding the need to record images at high tilt angles... - Convergent-beam low energy electron diffraction (CBLEED) and the measurement of surface dipole layersJ C H Spence
Department of Physics and Astronomy, College of Liberal Arts and Science, Arizona State University, Tempe, AZ 85287 1504, USA
Microsc Microanal 10:128-33. 2004..We briefly discuss also the application of this method to oxides and the formation of atomic-resolution scanning images in an idealized instrument in which coherent diffracted LEED orders overlap... - Coherence and sampling requirements for diffractive imagingJ C H Spence
Department of Physics and Astronomy, Arizona State University, Tempe, AZ 85287 1505, USA
Ultramicroscopy 101:149-52. 2004..The results are relevant to the design of diffraction cameras for CDI and plans for femotosecond X-ray diffraction from individual proteins... - Femtosecond protein nanocrystallography-data analysis methodsRichard A Kirian
Department of Physics, Arizona State University, Tempe, Arizona 85287 USA
Opt Express 18:5713-23. 2010..We determine the number of shots needed for a required accuracy in structure factor measurement and resolution, and investigate the convergence of our Monte-Carlo integration method... - Signal, noise, and resolution in correlated fluctuations from snapshot small-angle x-ray scatteringRichard A Kirian
Department of Physics, Arizona State University, Tempe, Arizona 85287, USA
Phys Rev E Stat Nonlin Soft Matter Phys 84:011921. 2011.... - Diffractive electron imaging of nanoparticles on a substrateJinsong Wu
Department of Physics and Astronomy, Arizona State University, Tempe, Arizona 85287 1504, USA
Nat Mater 4:912-6. 2005..The method has the advantage that the reconstructed exit-face wavefunction is free of the aberrations of the objective lens normally used in the microscope, whereas resolution is limited only by thermal vibration and noise... 
Structure-factor analysis of femtosecond microdiffraction patterns from protein nanocrystalsRichard A Kirian
Department of Physics, Arizona State University, Tempe, Arizona 85287, USA
Acta Crystallogr A 67:131-40. 2011..The implications for improving the efficiency of data collection by allowing the use of very small crystals, for radiation-damage reduction and for time-resolved diffraction studies at room temperature are discussed...- Damped and thermal motion of laser-aligned hydrated macromolecule beams for diffractionD Starodub
Department of Physics and Astronomy, Arizona State University, P O Box 871504 Tempe, Arizona 85287 1504, USA
J Chem Phys 123:244304. 2005.... - Dose, exposure time and resolution in serial X-ray crystallographyD Starodub
Department of Physics, Arizona State University, PO Box 871504, Tempe, AZ 85287 1504, USA
J Synchrotron Radiat 15:62-73. 2008.... - Femtosecond nanocrystallography using X-ray lasers for membrane protein structure determinationPetra Fromme
Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287 1604, USA
Curr Opin Struct Biol 21:509-16. 2011..We briefly review the possibilities for time-resolved crystallography, and the potential for making 'molecular movies' of membrane proteins at work... - Ordering energies and occupancies in doped TiAl, and dedicated ALCHEMI instrumentationJ C H Spence
Department of Physics and Astronomy, Arizona State University, Tempe, AZ, 85287-1504, USA
Microsc Microanal 8:241-6. 2002..The resulting occupancy information can provide essential input parameters for atomistic alloy design algorithms, and can provide entirely new information on interstitial occupancies in minerals, ceramics, semiconductors, and alloys... - In situ EELS study of dehydration of Al(OH)₃ by electron beam irradiationNan Jiang
Department of Physics, Arizona State University, Tempe, AZ 85287 1504, USA
Ultramicroscopy 111:860-4. 2011..The time-dependent O K-edge shows that the pre-edge peak in the O K-edge of Al(OH)₃ is induced by the formation of unpaired O, rather than an intrinsic feature of the OH bond... - Kinematic and dynamical CBED for solving thin organic films at low temperature; experimental tests with anthraceneJ S Wu
Department of Physics and Astronomy, Arizona State University, Tempe, AZ 85287-1504, USA
Acta Crystallogr A 58:580-9. 2002..The faint peaks due to H-atom positions may be distinguished. Key issues influencing the validity of the method such as the appropriate dimension of the structure-factor matrix, sample thickness and crystal orientation are discussed... - Phase recovery and lensless imaging by iterative methods in optical, X-ray and electron diffractionJ C H Spence
Department of Physics and Astronomy, Arizona State University, Tempe, AZ 85287-1504, USA
Philos Transact A Math Phys Eng Sci 360:875-95. 2002..These new interactions can be expected to provide new information, perhaps, for example, in biology, with the advantage of less damage to samples... - On the dose-rate threshold of beam damage in TEMNan Jiang
Department of Physics, Arizona State University, Tempe AZ 85287 1504, USA
Ultramicroscopy 113:77-82. 2012..The accumulation of damage can only occur when the damage rate is higher than the recovery rate. For surface sputtering process in TEM, the recovery rate is negligible. Therefore, there is no threshold dose rate for surface sputtering... - Phasing diffraction data from a stream of hydrated proteinsJin Song Wu
Department of Physics and Astronomy, Arizona State University, Tempe, Arizona 85287 1504, USA
J Opt Soc Am A Opt Image Sci Vis 22:1453-9. 2005..Rev. Lett. 92, 198102 (2004)]. We consider also the effect of empty ice balls on the patterns. The algorithm can also be used to align images with different randomly chosen origins, so that the same embedded subunits overlap... - On the measurement of thickness in nanoporous materials by EELSNan Jiang
Department of Physics, Arizona State University, Tempe, AZ 85287 1504, USA
Ultramicroscopy 111:62-5. 2010..In homogeneous nanoporous systems, on the other hand, the log-ratio method is still valid, but the inelastic scattering mean-free-path is no longer equal to that in the same bulk system... - SEM imaging of liquid jetsDaniel P DePonte
Department of Physics, Arizona State University, Tempe, AZ 85287, United States
Micron 40:507-9. 2009..ESEM imaging of liquid jets offers a valuable research tool for the study of aerosol production, combustion processes, ink-jet generation, and many other attributes of micro- and nanojet systems... - Observation of long-range compositional fluctuations in glasses: implications for atomic and electronic structureNan Jiang
Department of Physics and Astronomy, Arizona State University, Tempe, AZ 85287 1504, USA
Micron 39:698-702. 2008..Comparisons with spectra from compositionally equivalent crystals, and multiple-scattering calculations which include core-hole effects are used to analyze the data... - Single molecule diffractionJ C H Spence
Department of Physics and Astronomy, Arizona State University, Tempe, Arizona 85287-1504, USA
Phys Rev Lett 92:198102. 2004..Iterative methods for solving the phase problem are indicated. Comparisons with a related plan for pulsed x-ray diffraction from single proteins in a molecular beam are provided... - Reconstruction from a single diffraction pattern of azimuthally projected electron density of molecules aligned parallel to a single axisD K Saldin
Department of Physics, University of Wisconsin Milwaukee, PO Box 413, Milwaukee, WI 53201, USA
Acta Crystallogr A 66:32-7. 2010.... - Three-dimensional coherent x-ray diffraction imaging of a ceramic nanofoam: determination of structural deformation mechanismsA Barty
Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA 94550, USA
Phys Rev Lett 101:055501. 2008....