Petra Fromme

Summary

Affiliation: Arizona State University
Country: USA

Publications

  1. pmc High-resolution NMR reveals secondary structure and folding of amino acid transporter from outer chloroplast membrane
    James D Zook
    Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona, United States of America
    PLoS ONE 8:e78116. 2013
  2. pmc Femtosecond nanocrystallography using X-ray lasers for membrane protein structure determination
    Petra Fromme
    Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287 1604, USA
    Curr Opin Struct Biol 21:509-16. 2011
  3. ncbi request reprint Structure and function of photosystem I: interaction with its soluble electron carriers and external antenna systems
    Petra Fromme
    Department of Chemistry and Biochemistry, Arizona State University, P O Box 871604, Tempe, AZ 85287 1604, USA
    FEBS Lett 555:40-4. 2003
  4. ncbi request reprint Structure of Photosystems I and II
    Petra Fromme
    Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287 1604, USA
    Results Probl Cell Differ 45:33-72. 2008
  5. pmc Functional implications on the mechanism of the function of photosystem II including water oxidation based on the structure of photosystem II
    Petra Fromme
    Max Volmer Laboratorium für Biophysikalische Chemie und Biochemie, Technische Universitat Berlin, Strasse den 17 Juni 135, 10623 Berlin, Germany
    Philos Trans R Soc Lond B Biol Sci 357:1337-44; discussion 1344-5, 1367. 2002
  6. ncbi request reprint Structure of plant photosystem I revealed by theoretical modeling
    Craig Jolley
    Department of Physics and Astronomy, Arizona State University, Tempe, Arizona 85281 1504, USA
    J Biol Chem 280:33627-36. 2005
  7. pmc Conformational gating of dimannose binding to the antiviral protein cyanovirin revealed from the crystal structure at 1.35 A resolution
    Raimund Fromme
    Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287 1604, USA
    Protein Sci 17:939-44. 2008
  8. doi request reprint Isolation, folding and structural investigations of the amino acid transporter OEP16
    Da Qun Ni
    Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287 1604, USA
    Protein Expr Purif 80:157-68. 2011
  9. ncbi request reprint Characterization of a novel Photosystem I-LHCI supercomplex isolated from Chlamydomonas reinhardtii under anaerobic (State II) conditions
    Rajagopal Subramanyam
    School of Life Sciences and Center for the Study of Early Events in Photosynthesis, P O Box, 874501, Arizona State University, Tempe, AZ 85287 4501, USA
    FEBS Lett 580:233-8. 2006
  10. ncbi request reprint Docking of photosystem I subunit C using a constrained geometric simulation
    Craig C Jolley
    Department of Physics and Astronomy, The Center for the Study of Early Events in Photosynthesis, Arizona State University, Tempe, Arizona 85287, USA
    J Am Chem Soc 128:8803-12. 2006

Collaborators

Detail Information

Publications40

  1. pmc High-resolution NMR reveals secondary structure and folding of amino acid transporter from outer chloroplast membrane
    James D Zook
    Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona, United States of America
    PLoS ONE 8:e78116. 2013
    ..The results provide the necessary basis for high-resolution structural determination of this important plant membrane protein. ..
  2. pmc Femtosecond nanocrystallography using X-ray lasers for membrane protein structure determination
    Petra Fromme
    Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287 1604, USA
    Curr Opin Struct Biol 21:509-16. 2011
    ..We briefly review the possibilities for time-resolved crystallography, and the potential for making 'molecular movies' of membrane proteins at work...
  3. ncbi request reprint Structure and function of photosystem I: interaction with its soluble electron carriers and external antenna systems
    Petra Fromme
    Department of Chemistry and Biochemistry, Arizona State University, P O Box 871604, Tempe, AZ 85287 1604, USA
    FEBS Lett 555:40-4. 2003
    ..Furthermore, the potential interaction sites with the peripheral antenna proteins are discussed...
  4. ncbi request reprint Structure of Photosystems I and II
    Petra Fromme
    Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287 1604, USA
    Results Probl Cell Differ 45:33-72. 2008
    ..This chapter describes and compares the structure of two Photosystems and discusses their function in respect to the mechanism of light harvesting, electron transfer and water splitting...
  5. pmc Functional implications on the mechanism of the function of photosystem II including water oxidation based on the structure of photosystem II
    Petra Fromme
    Max Volmer Laboratorium für Biophysikalische Chemie und Biochemie, Technische Universitat Berlin, Strasse den 17 Juni 135, 10623 Berlin, Germany
    Philos Trans R Soc Lond B Biol Sci 357:1337-44; discussion 1344-5, 1367. 2002
    ..5 A resolution shows that photosystem II lacks the central domain of the photosystem I antenna, which is discussed in respect of the repair cycle of photosystem II due to photoinhibition...
  6. ncbi request reprint Structure of plant photosystem I revealed by theoretical modeling
    Craig Jolley
    Department of Physics and Astronomy, Arizona State University, Tempe, Arizona 85281 1504, USA
    J Biol Chem 280:33627-36. 2005
    ..Our model reveals some important structural features of plant PSI that were not visible in the crystal structure, and our model sheds light on the evolutionary relationship between plant and cyanobacterial PSI...
  7. pmc Conformational gating of dimannose binding to the antiviral protein cyanovirin revealed from the crystal structure at 1.35 A resolution
    Raimund Fromme
    Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287 1604, USA
    Protein Sci 17:939-44. 2008
    ....
  8. doi request reprint Isolation, folding and structural investigations of the amino acid transporter OEP16
    Da Qun Ni
    Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287 1604, USA
    Protein Expr Purif 80:157-68. 2011
    ..Recent collection of multidimensional NMR data at 500, 600 and 800 MHz demonstrated that the protein is suitable for structure determination by NMR and stable for weeks during data collection...
  9. ncbi request reprint Characterization of a novel Photosystem I-LHCI supercomplex isolated from Chlamydomonas reinhardtii under anaerobic (State II) conditions
    Rajagopal Subramanyam
    School of Life Sciences and Center for the Study of Early Events in Photosynthesis, P O Box, 874501, Arizona State University, Tempe, AZ 85287 4501, USA
    FEBS Lett 580:233-8. 2006
    ..These results indicate that there are large and dynamic changes of the PSI-LHCI supercomplex during the acclimation from aerobic (State I) to anaerobic (State II) conditions in Chlamydomonas...
  10. ncbi request reprint Docking of photosystem I subunit C using a constrained geometric simulation
    Craig C Jolley
    Department of Physics and Astronomy, The Center for the Study of Early Events in Photosynthesis, Arizona State University, Tempe, Arizona 85287, USA
    J Am Chem Soc 128:8803-12. 2006
    ..Simulations for two possible docking scenarios are explored, and experimental approaches to distinguish between them are discussed...
  11. pmc Data-directed top-down Fourier-transform mass spectrometry of a large integral membrane protein complex: photosystem II from Galdieria sulphuraria
    Balakumar Thangaraj
    Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 90024, USA
    Proteomics 10:3644-56. 2010
    ..Top-down high-resolution MS provides the necessary precision, typically less than 5 ppm, for identification and characterization of polypeptide composition of these important membrane protein complexes...
  12. doi request reprint A novel photosynthetic strategy for adaptation to low-iron aquatic environments
    Devendra Chauhan
    Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287, United States
    Biochemistry 50:686-92. 2011
    ..Excitation trapping and electron transfer are highly efficient, allowing cyanobacteria to avoid oxidative stress. This mechanism may be a major factor used by cyanobacteria to successfully adapt to modern low-Fe environments...
  13. pmc Fitting low-resolution cryo-EM maps of proteins using constrained geometric simulations
    Craig C Jolley
    Center for Biological Physics, Bateman Physical Sciences, Arizona State University, Tempe, Arizona, USA
    Biophys J 94:1613-21. 2008
    ..We show that by incorporating the correct local stereochemistry in the modeling, structures can be obtained with effective resolution that is significantly higher than might be expected from the nominal cryo-EM resolution...
  14. ncbi request reprint Low Temperature Assembly of Functional 3D DNA-PNA-Protein Complexes
    Justin D Flory
    Department of Chemistry and Biochemistry, Center for Bio Inspired Solar Fuel Production, and Biodesign Institute, Arizona State University, Tempe, Arizona 85287, United States
    J Am Chem Soc 136:8283-95. 2014
    ....
  15. doi request reprint PNA-peptide assembly in a 3D DNA nanocage at room temperature
    Justin D Flory
    Center for Bio Inspired Solar Fuel Production, Arizona State University, Tempe, Arizona 85287, United States
    J Am Chem Soc 135:6985-93. 2013
    ..This work demonstrates a flexible new approach to leverage rationally designed nucleic acid (DNA-PNA) nanoscaffolds to guide polypeptide engineering...
  16. ncbi request reprint A monovalent mutant of cyanovirin-N provides insight into the role of multiple interactions with gp120 for antiviral activity
    Raimund Fromme
    Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287 1604, USA
    Biochemistry 46:9199-207. 2007
    ..Our findings show that the presence of at least two oligomannoside-binding sites, either by the presence of intact domains A and B or by formation of domain-swapped dimers, is essential for activity...
  17. ncbi request reprint Structure of cyanobacterial photosystem I
    Ingo Grotjohann
    Arizona State University, Tempe, AZ, 85287 1604, USA
    Photosynth Res 85:51-72. 2005
    ..This review discusses the complex function of Photosystem I based on the structure of the complex at 2.5 A resolution as well as spectroscopic and biochemical data...
  18. doi request reprint Genetic analysis of the Photosystem I subunits from the red alga, Galdieria sulphuraria
    Christopher Vanselow
    Department of Chemistry and Biochemistry, Arizona State University, Tempe 85287 1604, AZ, USA
    Biochim Biophys Acta 1787:46-59. 2009
    ....
  19. pmc Crystallization and electron paramagnetic resonance characterization of the complex of photosystem I with its natural electron acceptor ferredoxin
    Petra Fromme
    Max Volmer Laboratorium, Institut fur Chemie, Fakultat II, Technische Universitat Berlin, Germany
    Biophys J 83:1760-73. 2002
    ..This simulation is consistent with the presence of 12 magnetically inequivalent F clusters per unit cell with the C(3) axis of the PSI trimers oriented at (23 degrees, 72 degrees, 77 degrees ) to the unit cell axes...
  20. pmc Toward structure determination using membrane-protein nanocrystals and microcrystals
    Mark S Hunter
    Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287, USA
    Methods 55:387-404. 2011
    ..The first proof-of-principle results for the femtosecond nanocrystallography technique indicate the incredible potential of the technique to offer a new route to the structure determination of membrane proteins...
  21. pmc Crystallization of a self-assembled three-dimensional DNA nanostructure
    Kimberly N Rendek
    Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287 1604, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 69:141-6. 2013
    ....
  22. pmc Efficient light harvesting in a dark, hot, acidic environment: the structure and function of PSI-LHCI from Galdieria sulphuraria
    Balakumar Thangaraj
    Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona, USA
    Biophys J 100:135-43. 2011
    ..This tight coupling helps Galdieria perform efficient light harvesting under the low-light conditions present in its natural endolithic habitat...
  23. pmc Crystallization of the large membrane protein complex photosystem I in a microfluidic channel
    Bahige G Abdallah
    Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287, United States
    ACS Nano 7:10534-43. 2013
    ..We postulate that this approach could be utilized to efficiently study and optimize crystallization conditions for a wide range of proteins that are poorly understood to date. ..
  24. pmc Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography
    Uwe Weierstall
    Department of Physics, Arizona State University, Tempe, Arizona 85287, USA
    Nat Commun 5:3309. 2014
    ..Data collected from sub-10-μm-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor...
  25. pmc Dielectrophoretic sorting of membrane protein nanocrystals
    Bahige G Abdallah
    Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287, United States
    ACS Nano 7:9129-37. 2013
    ..Microfluidic integration, continuous sorting, and nanometer-sized crystal fractions make this method ideal for direct coupling to femtosecond nanocrystallography...
  26. pmc Crystallization of the c14-rotor of the chloroplast ATP synthase reveals that it contains pigments
    Benjamin Varco-Merth
    Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona, USA 85287, USA
    Biochim Biophys Acta 1777:605-12. 2008
    ....
  27. pmc Structure-factor analysis of femtosecond microdiffraction patterns from protein nanocrystals
    Richard A Kirian
    Department of Physics, Arizona State University, Tempe, Arizona 85287, USA
    Acta Crystallogr A 67:131-40. 2011
    ..The implications for improving the efficiency of data collection by allowing the use of very small crystals, for radiation-damage reduction and for time-resolved diffraction studies at room temperature are discussed...
  28. pmc Femtosecond protein nanocrystallography-data analysis methods
    Richard A Kirian
    Department of Physics, Arizona State University, Tempe, Arizona 85287 USA
    Opt Express 18:5713-23. 2010
    ..We determine the number of shots needed for a required accuracy in structure factor measurement and resolution, and investigate the convergence of our Monte-Carlo integration method...
  29. pmc Purification and biophysical characterization of the CapA membrane protein FTT0807 from Francisella tularensis
    José M Martín-García
    Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287, United States
    Biochemistry 53:1958-70. 2014
    ..CapA is shown to be an inner-membrane protein and is predicted to play a key cellular role in the assembly of polysaccharides. ..
  30. pmc Folding kinetics and structure of OEP16
    Dirk Linke
    Max Volmer Laboratorium, Institut fur Chemie der Technischen Universitat Berlin, 10623 Berlin, Germany
    Biophys J 86:1479-87. 2004
    ....
  31. doi request reprint Recombinant production and purification of the subunit c of chloroplast ATP synthase
    Robert M Lawrence
    Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287 1604, USA
    Protein Expr Purif 76:15-24. 2011
    ..This work will enable further investigation into the undefined factors that affect the c-ring stoichiometry and structure. The c-subunit chosen for this work is that of spinach (Spinacia oleracea) chloroplast ATP synthase...
  32. ncbi request reprint Relevance of the diastereotopic ligation of magnesium atoms of chlorophylls in Photosystem I
    Teodor Silviu Balaban
    Forschungszentrum Karlsruhe, Institut fur Nanotechnologie, Postfach 3640, D 76021, Karlsruhe, Germany
    Biochim Biophys Acta 1556:197-207. 2002
    ..Results from semiempirical quantum mechanical and detailed exciton coupling calculations allow us to speculate on the functional relevance of the diasteretopicity for PSI functioning...
  33. ncbi request reprint Assembly of protein subunits within the stromal ridge of photosystem I. Structural changes between unbound and sequentially PS I-bound polypeptides and correlated changes of the magnetic properties of the terminal iron sulfur clusters
    Mikhail L Antonkine
    Institut fur Experimentalphysik, Freie Universitat Berlin, Arnimallee 14, D 14195 Berlin, Germany
    J Mol Biol 327:671-97. 2003
    ..The aim of this work is to relate contact information to structural changes in the proteins and to propose a model for the assembly of the stromal ridge of PS I based on this analysis...
  34. ncbi request reprint Excitation migration in trimeric cyanobacterial photosystem I
    Melih K Sener
    Beckman Institute, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    J Chem Phys 120:11183-95. 2004
    ..It is seen that on the average there is a nearly 40% chance of excitation cross transfer and trapping, indicating efficient coupling between monomers. The robustness and optimality of the chlorophyll network of trimeric PSI is examined...
  35. ncbi request reprint Size and shape determination of proteins in solution by a noninvasive depolarized dynamic light scattering instrument
    Naomi Chayen
    Division of Biomedical Sciences, Imperial College of Science, Technology and Medicine, London, UK
    Ann N Y Acad Sci 1027:20-7. 2004
    ..Its dimensions were determined to be 9.6 nm thick and 26 nm in diameter, values that are in good agreement with the dimensions obtained from X-ray diffraction analysis of single crystals...
  36. pmc Comparison of the light-harvesting networks of plant and cyanobacterial photosystem I
    Melih K Sener
    Beckman Institute, University of Illinois at Urbana Champaign, Urbana, Illinois, USA
    Biophys J 89:1630-42. 2005
    ..The finding suggests that after close packing of chlorophylls was achieved, constraints other than efficiency of the overall excitation transfer process precluded further evolution of pigment ordering...
  37. ncbi request reprint Red antenna states of photosystem I from cyanobacteria Synechocystis PCC 6803 and Thermosynechococcus elongatus: single-complex spectroscopy and spectral hole-burning study
    Kerry J Riley
    Department of Chemistry, Iowa State University, Ames, Iowa 50011, USA
    J Phys Chem B 111:286-92. 2007
    ..We also demonstrate that high-quality samples obtained by dissolving crystals of PS I of Thermosynechococcus elongatus exhibit stronger absorption in the red antenna region than any samples studied so far by us and other groups...
  38. pmc Light harvesting in photosystem I: modeling based on the 2.5-A structure of photosystem I from Synechococcus elongatus
    Martin Byrdin
    Institut fur Experimentalphysik, Freie Universitat Berlin, D 14195 Berlin, Germany
    Biophys J 83:433-57. 2002
    ..8 nm and k(CS) = 0.9 ps(-1). Variations of these parameters indicate that the excited state dynamics is neither pure trap limited, nor pure transfer (to-the-trap) limited but seems to be rather balanced...
  39. ncbi request reprint One- and two-color photon echo peak shift studies of photosystem I
    Harsha M Vaswani
    Department of Chemistry, University of California at Berkeley and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA
    J Phys Chem B 110:26303-12. 2006
    ..The simulations agree well with the experimental data. Two-color photon echo peak shift is shown to be far more sensitive to variations in the molecular Hamiltonian than one-color photon echo peak shift spectroscopy...