Research Topics
Genomes and Genes | C S GiomettiSummaryAffiliation: Argonne National Laboratory Country: USA Publications
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Publications
Transcriptional and proteomic analysis of a ferric uptake regulator (fur) mutant of Shewanella oneidensis: possible involvement of fur in energy metabolism, transcriptional regulation, and oxidative stressDorothea K Thompson
Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA
Appl Environ Microbiol 68:881-92. 2002..These results suggest a possible regulatory role of S. oneidensis MR-1 Fur in energy metabolism that extends the traditional model of Fur as a negative regulator of iron acquisition systems...
Knock-out of SO1377 gene, which encodes the member of a conserved hypothetical bacterial protein family COG2268, results in alteration of iron metabolism, increased spontaneous mutation and hydrogen peroxide sensitivity in Shewanella oneidensis MR-1Weimin Gao
Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA
BMC Genomics 7:76. 2006....
Global analysis of a "simple" proteome: Methanococcus jannaschiiCarol S Giometti
Biosciences Division, Argonne National Laboratory, 9700 South Cass Avenue, Building 202, Room B117, Argonne, IL 60439, USA
J Chromatogr B Analyt Technol Biomed Life Sci 782:227-43. 2002..jannaschii proteins has been found, as well as indications of incomplete dissociation of protein-protein complexes. These results demonstrate the complexity of proteome analysis even when dealing with a relatively simple genome...- Analysis of the Shewanella oneidensis proteome by two-dimensional gel electrophoresis under nondenaturing conditionsCarol S Giometti
Argonne National Laboratory, Biosciences Division, Argonne, IL 60439, USA
Proteomics 3:777-85. 2003..We have also found protein interactions within both the soluble and membrane fractions. The method described will be useful for the characterization of the functional proteomes of microbial systems... - Structural modifications of Methanococcus jannaschii flagellin proteins revealed by proteome analysisC S Giometti
Biosciences Division, Argonne National Laboratory, IL 60439, USA
Proteomics 1:1033-42. 2001..jannaschii and further suggest the regulation of M. jannaschii motility through structural modifications of the building blocks of the flagella... - Mouse liver selenium-binding protein decreased in abundance by peroxisome proliferatorsC S Giometti
Biosciences Division, Argonne Laboratory, IL 60439, USA
Electrophoresis 21:2162-9. 2000..Such a decrease in SBP, thought to serve as cell growth regulation factors, could be central to the nongenotoxic carcinogenicity of the peroxisome proliferators observed in rodents... - A comparison of liver protein changes in mice and hamsters treated with the peroxisome proliferator Wy-14,643C S Giometti
Center for Mechanistic Biology and Biotechnology, Argonne National Laboratory, IL 60439, USA
Electrophoresis 19:2498-505. 1998.... - A two-dimensional electrophoresis database of human breast epithelial cell proteinsC S Giometti
Center for Mechanistic Biology and Biotechnology, Argonne National Laboratory, IL 60439, USA
Electrophoresis 18:573-81. 1997..The World Wide Web presentation is designed to allow public access to the available 2-DE data together with logical connections to databases providing genome-related information... - Identification of a heritable deficiency of the folate-dependent enzyme 10-formyltetrahydrofolate dehydrogenase in miceK M Champion
Center for Mechanistic Biology and Biotechnology, Argonne National Laboratory, IL 60439 4833
Proc Natl Acad Sci U S A 91:11338-42. 1994..Measurement of hepatic folate pools showed that in homozygotes the total folate pool is decreased and the level of tetrahydrofolate is markedly depleted... - The analysis of recessive lethal mutations in mice by using two-dimensional gel electrophoresis of liver proteinsC S Giometti
Biological and Medical Research Division, Argonne National Laboratory, IL 60439
Mutat Res 242:47-55. 1990..No significant quantitative decreases in protein expression that correlated with the recessive lethal mutations were detected, however... - Mouse liver protein database: a catalog of proteins detected by two-dimensional gel electrophoresisC S Giometti
Biological and Medical Research Division, Argonne National Laboratory, IL 60439
Electrophoresis 13:970-91. 1992.... - GELBANK: a database of annotated two-dimensional gel electrophoresis patterns of biological systems with completed genomesGyorgy Babnigg
Protein Mapping Group, Biosciences Division, Argonne National Laboratory, Argonne, IL 60439, USA
Nucleic Acids Res 32:D582-5. 2004..The website currently presents 233 identifications for 81 gel patterns for Homo sapiens, Methanococcus jannaschii, Pyro coccus furiosus, Shewanella oneidensis, Escherichia coli and Deinococcus radiodurans... - ProteomeWeb: a web-based interface for the display and interrogation of proteomesGyorgy Babnigg
Biosciences Division, Argonne National Laboratory, Argonne, IL 60439, USA
Proteomics 3:584-600. 2003..The proteome of the archaeon Methanococcus jannaschii, a microbe for which the complete genome sequence is available, is used to demonstrate the capabilities of this evolving web interface (http://proteomeweb.anl.gov)... - Altered protein expression detected in the F1 offspring of male mice exposed to fission neutronsC S Giometti
Biological and Medical Research Division, Argonne National Laboratory, IL 60439
Mutat Res 320:75-85. 1994..These 2 mutations may thus represent mutation lesions other than point mutations (e.g., deletions or translocations) detectable as quantitative changes in protein expression in the F1 generation... - Tale of two metal reducers: comparative proteome analysis of Geobacter sulferreducens PCA and Shewanella oneidensis MR-1Carol S Giometti
Argonne National Laboratory, Argonne, Illinois, USA
Methods Biochem Anal 49:97-111. 2006..The end result, in the not too distant future, will be predictive models of G. sulfurreducens and S. oneidensis behavior in their natural habitats under a variety of environmental conditions... - Steady state protein levels in Geobacter metallireducens grown with iron (III) citrate or nitrate as terminal electron acceptorAngela J Ahrendt
Argonne National Laboratory, Biosciences Division, Argonne, IL, USA
Proteomics 7:4148-57. 2007..The oxidative stress response observed in previous studies of Geobacter sulfurreducens grown with Fe(III) citrate was not seen in G. metallireducens... - A combinatorial approach to studying protein complex composition by employing size-exclusion chromatography and proteome analysisShi Sheng Li
Biosciences Division, Argonne National Laboratory, Argonne, IL 60439, USA
J Sep Sci 30:1549-55. 2007..In one case, the partners of the B. subtilis protein complex have been coexpressed in Escherichia coli, and the formation of the overexpressed protein complex has been further confirmed by a pull-down assay... - A database of unique protein sequence identifiers for proteome studiesGyorgy Babnigg
Protein Mapping Group, Biosceinces Division, Argonne National Laboratory, IL 60439, USA
Proteomics 6:4514-22. 2006.... - Differential protein expression in the metal-reducing bacterium Geobacter sulfurreducens strain PCA grown with fumarate or ferric citrateTripti Khare
Argonne National Laboratory, Argonne, Illinois 60439, USA
Proteomics 6:632-40. 2006..Although an anaerobe, G. sulfurreducens appears to have alternate mechanisms for dealing with reactive oxygen species in response to increased intracellular soluble iron... - Proteomics and bioinformaticsCarol S Giometti
Argonne National Laboratory, Argonne, Illinois 60439, USA
Adv Protein Chem 65:353-69. 2003 - Differential recovery of biotinylated microbial proteins using monomeric or polymeric avidinTripti Khare
Argonne National Laboratory, Argonne, IL 60439, USA
Biotechniques 40:584, 586, 588. 2006 - The proteome of dissimilatory metal-reducing microorganism Geobacter sulfurreducens under various growth conditionsYan-Huai R Ding
Department of Microbiology, University of Massachusetts, Amherst, MA 01002, USA
Biochim Biophys Acta 1764:1198-206. 2006..sulfurreducens genome are produced and that the AMT tag approach provides a rapid method for comparing differential expression of proteins under different growth conditions in this organism... - Gene and protein expression profiles of Shewanella oneidensis during anaerobic growth with different electron acceptorsAlex S Beliaev
Environmental Sciences Division, Oak Ridge National Laboratory, Tennessee 37831-6038, USA
OMICS 6:39-60. 2002..Protein identification by mass spectrometry indicated that the expression of prismane, dihydrolipoamide succinyltransferase, and alcaligin siderophore biosynthesis protein correlated with the microarray data... - Global profiling of Shewanella oneidensis MR-1: expression of hypothetical genes and improved functional annotationsEugene Kolker
BIATECH, 19310 North Creek Parkway, Suite 115, Bothell, WA 98011, USA
Proc Natl Acad Sci U S A 102:2099-104. 2005..We propose that this integrative approach offers a valuable means to undertake the enormous challenge of characterizing the rapidly growing number of hypothetical proteins with each newly sequenced genome... - Shotgun proteomics of Methanococcus jannaschii and insights into methanogenesisWenhong Zhu
Department of Cell Biology, The Scripps Research Institute, 10550 N. Torrey Pines Rd, La Jolla, California 92037, USA
J Proteome Res 3:538-48. 2004..New peptides created by protein splicing were detected in MJ0885 (DNA dependent DNA polymerase), MJ0542, and MJ0782. The methanogenesis pathway and the enzymes involved are also discussed... - Identification of 2D-gel proteins: a comparison of MALDI/TOF peptide mass mapping to mu LC-ESI tandem mass spectrometryHanjo Lim
Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
J Am Soc Mass Spectrom 14:957-70. 2003..This improved sequence coverage in combination with enzymatic digestions of different specificity is proposed as a method for analysis of post-translational modification from 2D-gel separated proteins... - The influence of cultivation methods on Shewanella oneidensis physiology and proteome expressionDwayne A Elias
Department of Biochemistry, University of Missouri Columbia, Columbia, MO 65211, USA
Arch Microbiol 189:313-24. 2008..By decreasing cell to cell variability, higher quality samples will allow for the interpretive accuracy necessary for drawing conclusions relevant to microbial systems biology research... - Large-scale muLC-MS/MS for silver- and Coomassie blue-stained polyacrylamide gelsWenhong Zhu
Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA
Electrophoresis 26:4495-507. 2005..Although silver staining is not commonly thought to be optimal for MS analysis, protein identifications were successfully obtained from silver-stained 2-DE spots detected using methods with and without formaldehyde for protein fixation... - Low-temperature growth of Shewanella oneidensis MR-1Randa Abboud
Department of Biological Sciences, 3651 Trousdale Pkwy, AHF 107, University of Southern California, Los Angeles, CA 90089-0371, USA
Appl Environ Microbiol 71:811-6. 2005..Frequent pilus-like structures were observed during the transition from 3 to 22 degrees C... - Proteomic analysis of Psychrobacter cryohalolentis K5 during growth at subzero temperaturesCorien Bakermans
Center for Microbial Ecology, Michigan State University, East Lansing, MI 48824, USA
Extremophiles 11:343-54. 2007..In summary, these data suggest that low-temperature stresses continue to intensify as growth temperatures decrease to -4 degrees C...