Research Topics
| Caifu ChenSummaryAffiliation: Applied Biosystems Country: USA Publications
| Collaborators
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Detail Information
Publications
Real-time quantification of microRNAs by stem-loop RT-PCRCaifu Chen
Applied Biosystems, 850 Lincoln Centre Drive, Foster City, CA 94404, USA
Nucleic Acids Res 33:e179. 2005..Furthermore, the concept of stem-loop RT primer design could be applied in small RNA cloning and multiplex assays for better specificity and efficiency...
Gene expression in stem cellsYu Liang
Molecular Biology Systems Division, Life Technologies, Foster City, CA 94404, USA
Crit Rev Eukaryot Gene Expr 19:289-300. 2009..This review summarizes recent advances of gene expression analysis in stem cell research and also discusses critical issues that remain to be elucidated in the search for cancer stem cells markers...- The microRNA body map: dissecting microRNA function through integrative genomicsPieter Mestdagh
Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium and Life Technologies, Foster City, CA, USA
Nucleic Acids Res 39:e136. 2011..The miRNA bodymap project provides users with a single one-stop data-mining solution and has great potential to become a community resource... - The SNPlex genotyping system: a flexible and scalable platform for SNP genotypingAndreas R Tobler
Applied Biosystems, Foster City, CA 94404, USA
J Biomol Tech 16:398-406. 2005..The SNPlex Genotyping System is an efficient and reliable tool for a broad range of genotyping applications, supported by applications for study design, data analysis, and data management... 
Stem-loop RT-qPCR for microRNA expression profilingJames Hurley
Life Technologies Corporation, Foster City, CA, USA
Methods Mol Biol 822:33-52. 2012..Here, we demonstrate the use of both TaqMan(®) Array Card and OpenArray(®) platforms to accurately determine the level of miRNA gene expression in biological samples...- MicroRNAs in embryonic stem cellsYulei Wang
Gene Expression and Genotyping Assays R and D, Molecular Cell Biology Division, Applied Biosystems, Foster City, California, USA
J Cell Physiol 218:251-5. 2009..Here, we review recent progresses in characterizing the role of miRNAs in the maintenance and development of ESCs... - Defining embryonic stem cell identity using differentiation-related microRNAs and their potential targetsCaifu Chen
R and D, Applied Biosystems, Foster City, California 94404, USA
Mamm Genome 18:316-27. 2007..The concept of cellular identity has changed with technology; this study redefines cellular identity by a generic statistical method of known dimension... - Asynchronous PCRCaifu Chen
Genomic Assays R and D, Molecular Biology Systems Division, Life Technologies, Foster City, CA, USA
Methods Mol Biol 687:231-43. 2011..This new method can be used in real-time quantitative PCR (qPCR) for gene expression analyses as well as production of robust ssDNA targets for microarray and other hybridization-based applications... - Quantitation of microRNAs by real-time RT-qPCRCaifu Chen
Genomic Assays R and D, Molecular Biology Systems Division, Life Technologies, Foster City, CA, USA
Methods Mol Biol 687:113-34. 2011..Other methods such as poly (A) tailing-based and direct RT-based SYBR miRNA assays are also discussed in this chapter... - Characterization of microRNA expression profiles in normal human tissuesYu Liang
Molecular and Cell Biology R and D, Applied Biosystems, Foster City, CA 94404, USA
BMC Genomics 8:166. 2007..Matched samples from normal state can provide essential baseline references to analyze the variation of miRNA abundance... - Multiplexing RT-PCR for the detection of multiple miRNA species in small samplesKaiqin Lao
Applied Biosystems, 850 Lincoln Centre Dr, Foster City, CA 94404, USA
Biochem Biophys Res Commun 343:85-9. 2006..Here we investigate the parameters associated with multiplexing RT-PCR to obtain relative abundance profiles of multiple microRNAs in small sample sizes down to the amount of RNA found in a single cell... - MicroRNA and stem cell regulationYulei Wang
Life Technologies, Molecular Biology Systems Division, Foster City, CA 94404, USA
Curr Opin Mol Ther 11:292-8. 2009..This article reviews recent advances in the understanding of the roles of miRNAs in regulating various stem cell types, including embryonic stem cells, adult stem cells and CSCs... - Real-time PCR quantification of precursor and mature microRNAThomas D Schmittgen
College of Pharmacy, Ohio State University, Columbus, OH 43210, USA
Methods 44:31-8. 2008..Technological advancements have allowed for quantification of miRNA that is of comparable quality to more traditional RNAs... - Integrative genomic and functional analyses reveal neuronal subtype differentiation bias in human embryonic stem cell linesHao Wu
Mental Retardation Research Center, David Geffen School of Medicine at University of California, Los Angeles, Neuroscience Research Building Room 351, 635 Charles E Young Drive South, Los Angeles, CA 90095, USA
Proc Natl Acad Sci U S A 104:13821-6. 2007..Information on hESC line-specific differentiation biases is crucial for neural stem cell therapy and establishment of novel disease models using hESCs... - Identification of miRNA changes in Alzheimer's disease brain and CSF yields putative biomarkers and insights into disease pathwaysJohn P Cogswell
Department of Pharmacogenetics, GlaxoSmithKline Inc, Research Triangle Park, NC 27709, USA
J Alzheimers Dis 14:27-41. 2008..We additionally recovered miRNAs from cerebrospinal fluid and discovered AD-specific miRNA changes consistent with their role as potential biomarkers of disease... - Nonrestrictive developmental regulation of microRNA gene expressionWilliam M Strauss
MCD Biology, University of Colorado, Boulder, Colorado, 80309, USA
Mamm Genome 17:833-40. 2006.... - A recurrent 15q13.3 microdeletion syndrome associated with mental retardation and seizuresAndrew J Sharp
Department of Genome Sciences, University of Washington School of Medicine, 1705 NE Pacific St, Seattle, Washington 98195, USA
Nat Genet 40:322-8. 2008..The frequency of these microdeletions in mental retardation cases is approximately 0.3% (6/2,082 tested), a prevalence comparable to that of Williams, Angelman and Prader-Willi syndromes... - Characterization of microRNA expression levels and their biological correlates in human cancer cell linesArti Gaur
Norris Cotton Cancer Center, Dartmouth Medical School, Lebanon, New Hampshire 03755, USA
Cancer Res 67:2456-68. 2007..These findings call attention to the potential of microRNAs to provide etiologic insights as well as to serve as both diagnostic markers and therapeutic targets for many different tumor types... - A microRNA component of the p53 tumour suppressor networkLin He
Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA
Nature 447:1130-4. 2007..The p53 network suppresses tumour formation through the coordinated activation of multiple transcriptional targets, and miR-34 may act in concert with other effectors to inhibit inappropriate cell proliferation...