R M Hoffman


Affiliation: AntiCancer Inc
Country: USA


  1. Higuchi T, Kawaguchi K, Miyake K, Han Q, Tan Y, Oshiro H, et al. Oral Recombinant Methioninase Combined with Caffeine and Doxorubicin Induced Regression of a Doxorubicin-resistant Synovial Sarcoma in a PDOX Mouse Model. Anticancer Res. 2018;38:5639-5644 pubmed publisher
    ..These results suggest that combining o-rMETase and caffeine along with first-line chemotherapy can be highly effective for SS and has clinical potential for this recalcitrant disease. ..
  2. request reprint
    Yano S, Takehara K, Kishimoto H, Tazawa H, Urata Y, Kagawa S, et al. Comparison of Tumor Recurrence After Resection of Highly- and Poorly-Metastatic Triple-negative Breast Cancer in Orthotopic Nude-Mouse Models. Anticancer Res. 2017;37:57-60 pubmed
    ..The results of the present report suggest that conventional surgery under bright light was unable to control highly-metastatic compared with poorly-metastatic MDA-MB-231 TNBC. ..
  3. request reprint
    Igarashi K, Kawaguchi K, Kiyuna T, Murakami T, Miwa S, Nelson S, et al. Efficacy In Vitro of Caffeine and Valproic Acid on Patient-Derived Undifferentiated Pleomorphic Sarcoma and Rhabdomyosarcoma Cell Lines. Anticancer Res. 2017;37:4081-4084 pubmed
    ..13 ± 0.20 mM. Synergistic efficacy of combination treatment of CAF and VPA was also observed for both cell lines. The results of the present study suggest that CAF and VPA may be useful in the treatment of recalcitrant sarcoma. ..
  4. request reprint
    Matsumoto T, Suetsugu A, Shibata Y, Nakamura N, Aoki H, Kunisada T, et al. A Color-coded Imageable Syngeneic Mouse Model of Stromal-cell Recruitment by Metastatic Lymphoma. Anticancer Res. 2015;35:4647-54 pubmed
    ..This model of malignant lymphoma can be used to study early tumor development, metastasis, and the role of the stroma, as well as for discovery and evaluation of novel therapeutics for this treatment-resistant disease. ..
  5. Hoffman R, Yano S. Salmonella typhimurium A1-R and Cell-Cycle Decoy Therapy of Cancer. Methods Mol Biol. 2016;1409:165-75 pubmed publisher
    ..The S. typhimurium A1-R-decoyed cancer cells became sensitive to cytotoxic agents. ..
  6. request reprint
    Hasegawa K, Suetsugu A, Nakamura M, Matsumoto T, Kunisada T, Shimizu M, et al. Color-coded Imaging Enables Fluorescence-guided Surgery to Resect the Tumor Along with the Tumor Microenvironment in a Syngeneic Mouse Model of EL-4 Lymphoma. Anticancer Res. 2016;36:4443-8 pubmed
    ..Microscopically, cancer cells invade the TME and vice versa. To prevent tumor recurrence, it is necessary to resect the TME along with the tumor. ..
  7. request reprint
    Yano S, Takehara K, Kishimoto H, Tazawa H, Urata Y, Kagawa S, et al. In Vivo Selection of Intermediately- and Highly-Malignant Variants of Triple-negative Breast Cancer in Orthotopic Nude Mouse Models. Anticancer Res. 2016;36:6273-6277 pubmed
  8. request reprint
    Kiyuna T, Tome Y, Uehara F, Murakami T, Zhang Y, Zhao M, et al. Tumor-targeting Salmonella typhimurium A1-R Inhibits Osteosarcoma Angiogenesis in the In Vivo Gelfoam® Assay Visualized by Color-coded Imaging. Anticancer Res. 2018;38:159-164 pubmed
    ..typhimurium A1-R treatment by day 28. The present results indicate S. typhimurium A1-R has potential for anti-angiogenic targeted therapy of osteosarcoma. ..
  9. request reprint
    Matsumoto T, Suetsugu A, Hasegawa K, Nakamura M, Aoki H, Kunisada T, et al. Color-Coded Imaging of Syngeneic Orthotopic Malignant Lymphoma Interacting with Host Stromal Cells During Metastasis. Anticancer Res. 2016;36:1473-8 pubmed
    ..Color-coded imaging of the lymphoma microenvironment suggests an important role of stromal cells in lymphoma progression and metastasis. ..

More Information


  1. request reprint
    Hasegawa K, Suetsugu A, Nakamura M, Matsumoto T, Aoki H, Kunisada T, et al. Imaging Nuclear-Cytoplasmic Dynamics in Primary and Metastatic Colon Cancer in Nude Mice. Anticancer Res. 2016;36:2113-7 pubmed
    ..Our results demonstrate a similar heterogeneity of nuclear-cytoplasmic dynamics within primary tumors and metastases, which may be an important factor in the stochastic nature of metastasis. ..
  2. request reprint
    Igarashi K, Yamamoto N, Hayashi K, Takeuchi A, Kimura H, Miwa S, et al. Non-toxic Efficacy of the Combination of Caffeine and Valproic Acid on Human Osteosarcoma Cells In Vitro and in Orthotopic Nude-mouse Models. Anticancer Res. 2016;36:4477-82 pubmed
  3. request reprint
    Matsumoto T, Suetsugu A, Hasegawa K, Nakamura M, Shibata Y, Aoki H, et al. A Mouse Model of Fluorescent Protein-expressing Disseminated Peritoneal Lymphoma for Fluorescence-guided Surgery. Anticancer Res. 2016;36:4483-7 pubmed
    ..The present report indicates the feasibility of FGS of peritoneally-disseminated cancer. ..
  4. request reprint
    Igarashi K, Kawaguchi K, Kiyuna T, Murakami T, Yamamoto N, Hayashi K, et al. Antimetastatic Efficacy of the Combination of Caffeine and Valproic Acid on an Orthotopic Human Osteosarcoma Cell Line Model in Nude Mice. Anticancer Res. 2017;37:1005-1011 pubmed
    ..0005). Efficacy of the combination of caffeine and valproic acid was observed in vitro and in spontaneous and experimental lung-metastasis mouse models of osteosarcoma. ..
  5. request reprint
    Igarashi K, Kawaguchi K, Kiyuna T, Miyake K, Murakami T, Yamamoto N, et al. Effective Metabolic Targeting of Human Osteosarcoma Cells In Vitro and in Orthotopic Nude-mouse Models with Recombinant Methioninase. Anticancer Res. 2017;37:4807-4812 pubmed
    ..Future studies will investigate the efficacy of rMETase on patient-derived orthotopic xenograft (PDOX) models of osteosarcoma as a bridge to testing rMETase in the clinic. ..
  6. Hoffman R, Amoh Y. Beating Heart Cells from Hair-Follicle-Associated Pluripotent (HAP) Stem Cells. Methods Mol Biol. 2018;1842:241-254 pubmed publisher
    ..This method is appropriate for future use with human hair follicles to produce hHAP stem cells in sufficient quantities for future heart, nerve, and spinal cord regeneration in the clinic. ..
  7. Kiyuna T, Tome Y, Murakami T, Miyake K, Igarashi K, Kawaguchi K, et al. A combination of irinotecan/cisplatinum and irinotecan/temozolomide or tumor-targeting Salmonella typhimurium A1-R arrest doxorubicin- and temozolomide-resistant myxofibrosarcoma in a PDOX mouse model. Biochem Biophys Res Commun. 2018;: pubmed publisher
    ..typhimurium A1-R arrested the MFS PDOX tumor. There was no significant body weight loss in any group. The present study suggests that the combination of IRN with either TEM or CDDP, and S. typhimurium have clinical potential for MFS. ..
  8. Kimura H, Tome Y, Momiyama M, Hayashi K, Tsuchiya H, Bouvet M, et al. Imaging the inhibition by anti-?1 integrin antibody of lung seeding of single osteosarcoma cells in live mice. Int J Cancer. 2012;131:2027-33 pubmed publisher
    ..AIIB2 treatment also increased survival of mice with orthotopically growing 143B-RFP. ..
  9. request reprint
    Suetsugu A, Hiroshima Y, Matsumoto T, Hasagawa K, Nakamura M, Shimizu M, et al. Recruitment of Cancer-Associated Fibroblasts and Blood Vessels by Orthotopic Liver Tumors Imaged in Red Fluorescent Protein (RFP) Transgenic Nude Mice. Anticancer Res. 2015;35:5821-5 pubmed
  10. Kiyuna T, Tome Y, Murakami T, Kawaguchi K, Igarashi K, Miyake K, et al. Trabectedin arrests a doxorubicin-resistant PDGFRA-activated liposarcoma patient-derived orthotopic xenograft (PDOX) nude mouse model. BMC Cancer. 2018;18:840 pubmed publisher
    ..This is especially crucial for PLPS because effective first-line therapy is harder to establish if it is not individualized. ..
  11. Kiyuna T, Murakami T, Tome Y, Igarashi K, Kawaguchi K, Miyake K, et al. Doxorubicin-resistant pleomorphic liposarcoma with PDGFRA gene amplification is targeted and regressed by pazopanib in a patient-derived orthotopic xenograft mouse model. Tissue Cell. 2018;53:30-36 pubmed publisher
  12. Hoffman R. 3D Sponge-Matrix Histoculture: An Overview. Methods Mol Biol. 2018;1760:11-17 pubmed publisher
  13. Hoffman R, Mii S, Duong J, Amoh Y. Nerve Growth and Interaction in Gelfoam® Histoculture: A Nervous System Organoid. Methods Mol Biol. 2018;1760:163-186 pubmed publisher
    ..HAP stem cell proliferation resulted in elongation of the follicle nerve and interaction with other nerves in 3D Gelfoam® histoculture, including the sciatic nerve, trigeminal nerve, and trigeminal nerve ganglion. ..
  14. Hoffman R. Nestin-driven green fluorescent protein as an imaging marker for nascent blood vessels in mouse models of cancer. Methods Mol Biol. 2011;689:183-204 pubmed publisher
    ..These fluorescent models can be used to study the early events of angiogenesis and to quantitatively determine efficacy of antiangiogenesis compounds. ..
  15. request reprint
    Hoffman R, Yang M. Color-coded fluorescence imaging of tumor-host interactions. Nat Protoc. 2006;1:928-35 pubmed
    ..The 'color-coded' tumor-host models enable imaging and therefore a deeper understanding of the host cells involved and their function in tumor progression. Approximately 4-8 weeks are needed for these procedures. ..
  16. Hoffman R, Freeman A. In Vivo-Like Growth Patterns of Multiple Types of Tumors in Gelfoam® Histoculture. Methods Mol Biol. 2018;1760:19-28 pubmed publisher
  17. Kawaguchi K, Igarashi K, Miyake K, Lwin T, Miyake M, Kiyuna T, et al. MEK inhibitor trametinib in combination with gemcitabine regresses a patient-derived orthotopic xenograft (PDOX) pancreatic cancer nude mouse model. Tissue Cell. 2018;52:124-128 pubmed publisher
    ..However, GEM?+?TRA inhibited the PDOX tumor growth significantly greater than TRA alone. These results suggest the clinical potential of the combination of TRA and GEM for pancreatic cancer. ..
  18. Kawaguchi K, Miyake K, Han Q, Li S, Tan Y, Igarashi K, et al. Oral recombinant methioninase (o-rMETase) is superior to injectable rMETase and overcomes acquired gemcitabine resistance in pancreatic cancer. Cancer Lett. 2018;432:251-259 pubmed publisher
    ..The present study shows that o-rMETase is effective and overcomes acquired GEM resistance in pancreatic cancer and demonstrates the clinical potential of this strategy. ..
  19. Hoffman R, Bouvet M. Nanoparticle albumin-bound-paclitaxel: a limited improvement under the current therapeutic paradigm of pancreatic cancer. Expert Opin Pharmacother. 2015;16:943-7 pubmed publisher
  20. Hoffman R. Human Hair Follicle Associated-Pluripotent (hHAP) Stem Cells Differentiate to Cardiac Muscle Cells. Methods Mol Biol. 2018;: pubmed publisher
    ..The methods of this chapter are appropriate for use of human hair follicles to produce hHAP stem cells in sufficient quantities for future heart, nerve, and spinal cord regeneration in the clinic. ..
  21. Hoffman R, Yano S. In Vivo-Like Cell-Cycle Phase Distribution of Cancer Cells in Gelfoam® Histoculture Observed in Real Time by FUCCI Imaging. Methods Mol Biol. 2018;1760:109-123 pubmed publisher
    ..In 2D culture, cancer cells were much more chemosensitive. These results indicate why the drug response pattern of tumors in Gelfoam® histoculture reflects what is observed in vivo. ..
  22. Hoffman R. Diagnosis and Pathological Analysis of Patient Cancers by Detection of Proliferating Cells in Gelfoam® Histoculture. Methods Mol Biol. 2018;1760:49-60 pubmed publisher
    ..Normal tissues can also proliferate at high rates. Mean growth fraction index (GFI) was highest for patient tumors with the pure subtype of small-cell lung cancer than other types of lung cancer. ..
  23. Hoffman R, Vescio R. Development of the Histoculture Drug Response Assay (HDRA). Methods Mol Biol. 2018;1760:39-48 pubmed publisher
  24. request reprint
    Suetsugu A, Snyder C, Moriwaki H, Saji S, Bouvet M, Hoffman R. Imaging the Interaction of Pancreatic Cancer and Stellate Cells in the Tumor Microenvironment during Metastasis. Anticancer Res. 2015;35:2545-51 pubmed
    ..Pancreatic cancer cells and stellate stem cells form a very close relationship and accompany each other to distant metastatic sties. Our hypothesis is that pancreatic stellate cells form a niche for metastasis of pancreatic cancer. ..
  25. Hoffman R. Clinical Usefulness of the Histoculture Drug Response Assay for Prostate Cancer and Benign Prostate Hypertrophy (BPH). Methods Mol Biol. 2018;1760:101-107 pubmed publisher
    ..Genistein decreased the androgen-sensitivity index of BPH and prostate cancer in Gelfoam® histoculture in a dose-dependent manner. ..
  26. Igarashi K, Kawaguchi K, Kiyuna T, Miyake K, Miyake M, Singh A, et al. Tumor-targeting Salmonella typhimurium A1-R is a highly effective general therapeutic for undifferentiated soft tissue sarcoma patient-derived orthotopic xenograft nude-mouse models. Biochem Biophys Res Commun. 2018;497:1055-1061 pubmed publisher
    ..These results suggest that S. typhimurium A1-R can be a general therapeutic for USTS and possibly sarcoma in general. ..
  27. Liu F, Hoffman R. Hair Follicle-Associated Pluripotent (HAP) Stem Cells in Gelfoam® Histoculture for Use in Spinal Cord Repair. Methods Mol Biol. 2018;1760:145-162 pubmed publisher
    ..Twenty-eight days after transplantation with BA or DP HAP stem cells on Gelfoam® to the injured area of the spine, the mice recovered normal locomotion. ..
  28. request reprint
    Zhang Y, Zhang N, Zhao M, Hoffman R. Real-time Non-invasive Spectral Imaging of Orthotopic Red Fluorescent Protein-expressing Lung Tumor Growth in Nude Mice. Anticancer Res. 2015;35:3755-9 pubmed
    ..This imageable nude mouse model using surgical orthotopic implantation of lung cancer will be useful for all types of longitudinal studies. ..
  29. Hoffman R, Jung P, Kim M, Nam J. Prospective Clinical Correlation of the Histoculture Drug Response Assay for Ovarian Cancer. Methods Mol Biol. 2018;1760:73-81 pubmed publisher
    ..The true-positive rate was 88%, the true-negative rate was 86%, the sensitivity was 88%, the specificity was 86%, and the accurate prediction rate was 87% when HDRA results were compared to the response of the treated patients. ..
  30. Hoffman R. Methionine Dependency Determination of Human Patient Tumors in Gelfoam® Histoculture. Methods Mol Biol. 2018;1760:125-131 pubmed publisher
    ..This simple method can be used to screen patient tumors for methionine dependence and then subsequently apply appropriate chemotherapy for these patients to target this cancer-specific metabolic abnormality. ..
  31. Hoffman R. Development of recombinant methioninase to target the general cancer-specific metabolic defect of methionine dependence: a 40-year odyssey. Expert Opin Biol Ther. 2015;15:21-31 pubmed publisher
  32. request reprint
    Hiroshima Y, Zhang Y, Zhang N, Uehara F, Maawy A, Murakami T, et al. Patient-derived orthotopic xenograft (PDOX) nude mouse model of soft-tissue sarcoma more closely mimics the patient behavior in contrast to the subcutaneous ectopic model. Anticancer Res. 2015;35:697-701 pubmed
    ..In contrast, the majority of the subcutaneously-implanted tumor comprised round to oval cells. These results indicate that the PDOX recapitulated the histology of the original tumor more than the subcutaneous model. ..
  33. Hoffman R, Chishima T. Imaging the Governing Step of Metastasis in Gelfoam® Histoculture. Methods Mol Biol. 2018;1760:215-220 pubmed publisher
    ..The ability to support selective organ colonization in Gelfoam® histoculture and visualize tumor progression by GFP fluorescence allows the in vitro study of the governing processes of metastasis. ..
  34. Hoffman R. Orthotopic mouse models of tumor metastasis expressing fluorescent reporters produce imageable circulating tumor cells. Cancer Microenviron. 2014;7:133-8 pubmed publisher
    ..We also describe a facile method to label patient CTCs ex vivo using a telomerase-expressing GFP-containing adenovirus that will allow the CTC studies described in this review to be translated clinically. ..
  35. request reprint
    Uehara F, Tome Y, Yano S, Miwa S, Mii S, Hiroshima Y, et al. A color-coded imaging model of the interaction of ?v integrin-GFP expressed in osteosarcoma cells and RFP expressing blood vessels in Gelfoam® vascularized in vivo. Anticancer Res. 2013;33:1361-6 pubmed
    ..The observation of the behavior of ?v integrin-GFP and blood vessels will allow further understanding of the role of ?v integrin in cancer cells. ..
  36. Hoffman R. Tumor-targeting amino acid auxotrophic Salmonella typhimurium. Amino Acids. 2009;37:509-21 pubmed publisher
    ..Exploitation of the tumor-killing capability of Salmonella has great promise for a new paradigm of cancer therapy. ..
  37. Hoffman R. Clinical Correlation of the Histoculture Drug Response Assay in Gastrointestinal Cancer. Methods Mol Biol. 2018;1760:61-72 pubmed publisher
  38. request reprint
    Yamamoto N, Gupta A, Xu M, Miki K, Tsujimoto Y, Tsuchiya H, et al. Methioninase gene therapy with selenomethionine induces apoptosis in bcl-2-overproducing lung cancer cells. Cancer Gene Ther. 2003;10:445-50 pubmed
    ..rAdMET/SeMET prodrug gene therapy is therefore a promising novel strategy effective against bcl-2 overexpression, which has blocked other gene therapy strategies. ..
  39. Yano S, Miwa S, Kishimoto H, Toneri M, Hiroshima Y, Yamamoto M, et al. Experimental Curative Fluorescence-guided Surgery of Highly Invasive Glioblastoma Multiforme Selectively Labeled With a Killer-reporter Adenovirus. Mol Ther. 2015;23:1182-1188 pubmed publisher
    ..OBP-401-based FGS enabled curative resection of GBM without recurrence for at least 150 days, compared to less than 30 days with BLS. ..
  40. request reprint
    Suetsugu A, Jiang P, Yang M, Yamamoto N, Moriwaki H, Saji S, et al. The Use of Living Cancer Cells Expressing Green Fluorescent Protein in the Nucleus and Red Fluorescence Protein in the Cytoplasm for Real-time Confocal Imaging of Chromosome and Cytoplasmic Dynamics During Mitosis. Anticancer Res. 2015;35:2553-7 pubmed
    ..The dual color cells will be a useful tool to discover and evaluate novel strategies for killing cancer cells. ..
  41. request reprint
    Zhang Y, Zhang N, Hoffman R, Zhao M. Surgically-Induced Multi-organ Metastasis in an Orthotopic Syngeneic Imageable Model of 4T1 Murine Breast Cancer. Anticancer Res. 2015;35:4641-6 pubmed
    ..The metastatic pattern in the model thus reflected the clinical metastatic pattern of breast cancer and should be of use for discovery and evaluation of novel therapeutics. ..
  42. Tome Y, Uehara F, Kanaya F, Hoffman R. Comparison of "Dimensionality" of Cancer Cell Culture in Gelfoam® Histoculture and Matrigel. Methods Mol Biol. 2018;1760:205-214 pubmed publisher
    ..When the cancer cells were seeded on Gelfoam®, the cells formed 3-dimensional tissue-like structures. These results indicate that Gelfoam® histoculture, unlike Matrigel™ culture, is true 3-dimensional. ..
  43. request reprint
    Hoffman R, Yang M. Whole-body imaging with fluorescent proteins. Nat Protoc. 2006;1:1429-38 pubmed
    ..Fluorescent protein-based imaging technology can be used for whole-body imaging of fluorescent cells on essentially all organs. The timeline for these experiments varies from 2 days to 2 months. ..
  44. Hoffman R. Back to the Future: Are Tumor-Targeting Bacteria the Next-Generation Cancer Therapy?. Methods Mol Biol. 2015;1317:239-60 pubmed publisher
    ..A new concept using Salmonella typhimurium A1-R for cell cycle "decoy" chemotherapy of metastatic cancer is also described. ..
  45. Hoffman R. Clinical Correlation of the Histoculture Drug Response Assay for Head and Neck Cancer. Methods Mol Biol. 2018;1760:83-92 pubmed publisher
    ..64%), CDDP (86% vs. 64%), or both agents (85% vs. 63%). These results demonstrate the clinical usefulness of the HDRA for head and neck cancer. ..
  46. request reprint
    Yang M, Jiang P, Hoffman R. Early Reporting of Apoptosis by Real-time Imaging of Cancer Cells Labeled with Green Fluorescent Protein in the Nucleus and Red Fluorescent Protein in the Cytoplasm. Anticancer Res. 2015;35:2539-43 pubmed
    ..The dual-color cell real-time imaging assay is a more sensitive and earlier reporter for apoptosis than the DNA fragmentation assay. ..
  47. Hoffman R, Tanino H. Clinical Usefulness of the Histoculture Drug Response Assay for Breast Cancer. Methods Mol Biol. 2018;1760:93-100 pubmed publisher
    ..There also was a lack of correlation with breast cancer subtype and drug response in the HDRA, further suggesting the importance of individualized treatment for breast cancer patients afforded by the HDRA. ..
  48. Hoffman R, Li L, Cao W. Hair-Shaft Growth in Gelfoam® Histoculture of Skin and Isolated Hair Follicles. Methods Mol Biol. 2018;1760:133-144 pubmed publisher
  49. Miwa S, Hoffman R. Imaging DNA Repair After UV Irradiation Damage of Cancer Cells in Gelfoam® Histoculture. Methods Mol Biol. 2018;1760:199-203 pubmed publisher
    ..Induction of UV-induced 53BP1-GFP focus formation was limited up to a depth of 40 μm in Gelfoam® histoculture of MiaPaCa-2 cells, indicating this was the depth limit of UVC irradiation. ..
  50. request reprint
    Zhang Y, Hiroshima Y, Ma H, Zhang N, Zhao M, Hoffman R. Complementarity of variable-magnification and spectral-separation fluorescence imaging systems for noninvasive detection of metastasis and intravital detection of single cancer cells in mouse models. Anticancer Res. 2015;35:661-7 pubmed
  51. Hoffman R, Guadagni F. Expression and Targeting of Tumor Markers in Gelfoam® Histoculture: Potential Individualized Assays for Immuno-Oncology. Methods Mol Biol. 2018;1760:29-37 pubmed publisher
  52. request reprint
    Uchugonova A, Zhang Y, Salz R, Liu F, Suetsugu A, Zhang L, et al. Imaging the Different Mechanisms of Prostate Cancer Cell-killing by Tumor-targeting Salmonella typhimurium A1-R. Anticancer Res. 2015;35:5225-9 pubmed
    ..Understanding the various mechanisms of cancer-cell killing by S. typhimurium A1-R will be important for its use as a general therapeutic for cancer. ..
  53. Hoffman R. Future of Bacterial Therapy of Cancer. Methods Mol Biol. 2016;1409:177-84 pubmed publisher
    ..This chapter suggests future clinical applications for S. typhimurium A1-R. ..
  54. request reprint
    Suetsugu A, Matsumoto T, Hasegawa K, Nakamura M, Kunisada T, Shimizu M, et al. Color-coded Live Imaging of Heterokaryon Formation and Nuclear Fusion of Hybridizing Cancer Cells. Anticancer Res. 2016;36:3827-31 pubmed
    ..The ability to image the complex processes following cell fusion using reciprocal color-coded cancer cells will allow greater understanding of the genetic basis of malignancy. ..
  55. Hoffman R, Zhao M. Methods for Tumor Targeting with Salmonella typhimurium A1-R. Methods Mol Biol. 2016;1409:143-64 pubmed publisher
    ..The testing of S. typhimurium A1-R on patient tumors in patient-derived orthotopic xenograft (PDOX) mouse models is also described. The major methodologies described in this chapter should be translatable for clinical studies. ..
  56. request reprint
    Hoffman R, Kajiura S, Cao W, Liu F, Amoh Y. Cryopreservation of Hair-Follicle Associated Pluripotent (HAP) Stem Cells Maintains Differentiation and Hair-Growth Potential. Adv Exp Med Biol. 2016;951:191-198 pubmed
    ..HAP stem cells can be used for nerve and spinal-cord repair. This biobanking of hair follicles can allow each patient the potential for their own stem cell use for regenerative medicine or hair transplantation. ..
  57. Miwa S, Toneri M, Igarashi K, Yano S, Kimura H, Hayashi K, et al. Real-Time In Vivo Confocal Fluorescence Imaging of Prostate Cancer Bone-Marrow Micrometastasis Development at the Cellular Level in Nude Mice. J Cell Biochem. 2016;117:2533-7 pubmed publisher
    ..J. Cell. Biochem. 117: 2533-2537, 2016. © 2016 Wiley Periodicals, Inc. ..
  58. Zhang Y, Toneri M, Ma H, Yang Z, Bouvet M, Goto Y, et al. Real-Time GFP Intravital Imaging of the Differences in Cellular and Angiogenic Behavior of Subcutaneous and Orthotopic Nude-Mouse Models of Human PC-3 Prostate Cancer. J Cell Biochem. 2016;117:2546-51 pubmed publisher
    ..These results explain why orthotopic tumors mimimc clinical metastatic tumors in nude mice and why subcutaneous tumors do not. J. Cell. Biochem. 117: 2546-2551, 2016. © 2016 Wiley Periodicals, Inc. ..
  59. Cao W, Liu F, Amoh Y, Hoffman R. Protocols for Gelfoam(®) Histoculture of Hair-Shaft-Producing Mouse Whisker Follicles Containing Nestin-GFP-Expressing Hair-Follicle-Associated Pluripotent (HAP) Stem Cells for Long Time Periods. Methods Mol Biol. 2016;1453:145-50 pubmed publisher
    ..Three-dimensional Gelfoam(®) histoculture of hair follicles can provide a very long-term period for evaluating novel agents to promote hair growth. ..
  60. Cao W, Liu F, Amoh Y, Hoffman R. Protocols for Ectopic Hair Growth from Transplanted Whisker Follicles on the Spinal Cord of Mice. Methods Mol Biol. 2016;1453:137-44 pubmed publisher
    ..This result changes our concept of hair growth, demonstrating it is not limited to the skin and that hair growth appears related to HAP stem cells as both increased in tandem on the spinal cord. ..
  61. Hoffman R. Tumor-Targeting Salmonella typhimurium A1-R: An Overview. Methods Mol Biol. 2016;1409:1-8 pubmed publisher
  62. Hoffman R, Bouvet M. Imaging the microenvironment of pancreatic cancer patient-derived orthotopic xenografts (PDOX) growing in transgenic nude mice expressing GFP, RFP, or CFP. Cancer Lett. 2016;380:349-55 pubmed publisher
    ..This model enables powerful color-coded imaging of the interaction of cancer and stromal cells during tumor progression and treatment. ..
  63. request reprint
    Murakami T, Zhang Y, Wang X, Hiroshima Y, Kasashima H, Yashiro M, et al. Orthotopic Implantation of Intact Tumor Tissue Leads to Metastasis of OCUM-2MD3 Human Gastric Cancer in Nude Mice Visualized in Real Time by Intravital Fluorescence Imaging. Anticancer Res. 2016;36:2125-30 pubmed
    ..These results demonstrate the physiological importance of intact cancer tissue for orthotopic implantation in order for tumors to properly grow and express their metastatic potential. ..
  64. Li L, Hoffman R. Discovery of HAP Stem Cells. Methods Mol Biol. 2016;1453:15-20 pubmed publisher
    ..The ND-GFP hair follicle stem cells were later termed hair-follicle-associated pluripotent (HAP) stem cells. ..
  65. Hoffman R. Use of fluorescent proteins and color-coded imaging to visualize cancer cells with different genetic properties. Cancer Metastasis Rev. 2016;35:5-19 pubmed publisher
    ..These properties also demonstrate the vast superiority of imaging cancer cells in vivo with fluorescent proteins over photon counting of luciferase-labeled cancer cells. ..
  66. Hoffman R. Introduction to Hair-Follicle-Associated Pluripotent Stem Cells. Methods Mol Biol. 2016;1453:1-5 pubmed publisher
  67. Igarashi K, Kawaguchi K, Li S, Han Q, Tan Y, Murakami T, et al. Recombinant methioninase in combination with doxorubicin (DOX) overcomes first-line DOX resistance in a patient-derived orthotopic xenograft nude-mouse model of undifferentiated spindle-cell sarcoma. Cancer Lett. 2018;417:168-173 pubmed publisher
    ..The present results demonstrate the power of the PDOX model to identify effective therapy for recalcitrant cancer and the potential of rMETase to overcome DOX resistance. ..
  68. Amoh Y, Katsuoka K, Hoffman R. Peripheral-Nerve and Spinal-Cord Regeneration in Mice Using Hair-Follicle-Associated Pluripotent (HAP) Stem Cells. Methods Mol Biol. 2016;1453:21-32 pubmed publisher
    ..These results suggest that HAP stem cells provide an important accessible, autologous source of adult stem cells for regenerative medicine, that have critical advantages over ES and iPS stem cells. ..
  69. request reprint
    Tome Y, Yano S, Sugimoto N, Mii S, Uehara F, Miwa S, et al. Use of ?v Integrin Linked to Green Fluorescent Protein in Osteosarcoma Cells and Confocal Microscopy to Image Molecular Dynamics During Lung Metastasis in Nude Mice. Anticancer Res. 2016;36:3811-6 pubmed
    ..The results also suggest a general concept for imaging molecular behavior in vivo. ..
  70. request reprint
    Yano S, Takehara K, Miwa S, Kishimoto H, Tazawa H, Urata Y, et al. In Vivo Isolation of a Highly-aggressive Variant of Triple-negative Human Breast Cancer MDA-MB-231 Using Serial Orthotopic Transplantation. Anticancer Res. 2016;36:3817-20 pubmed
    ..The availability of a highly invasive variant of TNBC targeting lymph nodes will be very useful for drug discovery of TNBC, a recalcitrant cancer and for mechanistic studies of its aggressiveness. ..
  71. request reprint
    Tome Y, Uehara F, Miwa S, Yano S, Mii S, Efimova E, et al. Efficacy of the Combination of a PARP Inhibitor and UVC on Cancer Cells as Imaged by Focus Formation by the DNA Repair-related Protein 53BP1 Linked to Green Fluorescent Protein. Anticancer Res. 2016;36:3821-6 pubmed
    ..Confocal microscopy of 53BP1-GFP is a powerful method for imaging UVC-induced DNA damage and repair, as well as inhibition of repair. ..
  72. request reprint
    Tome Y, Kimura H, Maehara H, Sugimoto N, Bouvet M, Tsuchiya H, et al. High lung-metastatic variant of human osteosarcoma cells, selected by passage of lung metastasis in nude mice, is associated with increased expression of ?(v)?(3) integrin. Anticancer Res. 2013;33:3623-7 pubmed
    ..With this highly metastatic variant overexpressing ?v?3 integrin, it will now be possible to further investigate the mechanism by which ?v?3 integrin facilitates metastasis. ..
  73. request reprint
    Tome Y, Sugimoto N, Yano S, Momiyama M, Mii S, Maehara H, et al. Real-time imaging of ?v integrin molecular dynamics in osteosarcoma cells in vitro and in vivo. Anticancer Res. 2013;33:3021-5 pubmed
    ..These results show that ?v integrin-GFP enables the imaging of the molecular dynamics of ?v integrin in osteosarcoma cells in vitro and in vivo. ..
  74. Hoffman R. Patient-Derived Orthotopic Xenograft (PDOX) Models of Melanoma. Int J Mol Sci. 2017;18: pubmed publisher
    ..Promising results have been obtained with regard to identifying effective approved agents and experimental therapeutics, as well as combinations of the two using the melanoma PDOX model. ..