R M Hoffman

Summary

Affiliation: AntiCancer Inc
Country: USA

Publications

  1. ncbi request reprint Orthotopic metastatic (MetaMouse) models for discovery and development of novel chemotherapy
    Robert M Hoffman
    Department Surgery, University of California San Diego, and AntiCancer Inc, USA
    Methods Mol Med 111:297-322. 2005
  2. doi request reprint In vivo real-time imaging of nuclear-cytoplasmic dynamics of dormancy, proliferation and death of cancer cells
    Robert M Hoffman
    AntiCancer, Inc and Department of Surgery, University of California at San Diego, San Diego, CA 92111 3604, USA
    APMIS 116:716-29. 2008
  3. ncbi request reprint Dual-color imaging of nascent angiogenesis and its inhibition in liver metastases of pancreatic cancer
    Yasuyuki Amoh
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, California, USA
    Anticancer Res 26:3237-42. 2006
  4. ncbi request reprint Transgenic nude mice ubiquitously expressing fluorescent proteins for color-coded imaging of the tumor microenvironment
    Robert M Hoffman
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA, 92111, USA
    Methods Mol Biol 1194:353-65. 2014
  5. ncbi request reprint Methods for the development of tumor-targeting bacteria
    Robert M Hoffman
    AntiCancer, Inc, San Diego, CA, USA
    Expert Opin Drug Discov 9:741-50. 2014
  6. pmc Development of a clinically-precise mouse model of rectal cancer
    Hiroyuki Kishimoto
    AntiCancer, Inc, San Diego, California, United States of America Department of Surgery, University of California San Diego, San Diego, California, United States of America Division of Surgical Oncology, Department of Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan
    PLoS ONE 8:e79453. 2013
  7. ncbi request reprint Imaging nuclear - cytoplasm dynamics of cancer cells in the intravascular niche of live mice
    Atsushi Suetsugu
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111, U S A
    Anticancer Res 33:4229-36. 2013
  8. ncbi request reprint High lung-metastatic variant of human osteosarcoma cells, selected by passage of lung metastasis in nude mice, is associated with increased expression of α(v)β(3) integrin
    Yasunori Tome
    AntiCancer Inc, 7917 Ostrow Street, San Diego, CA 92111, U S A
    Anticancer Res 33:3623-7. 2013
  9. ncbi request reprint Real-time imaging of αv integrin molecular dynamics in osteosarcoma cells in vitro and in vivo
    Yasunori Tome
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111, USA
    Anticancer Res 33:3021-5. 2013
  10. ncbi request reprint Color-coded imaging of spontaneous vessel anastomosis in vivo
    Fuminari Uehara
    AntiCancer Inc, 7917 Ostrow Street, San Diego, California 92111, USA
    Anticancer Res 33:3041-5. 2013

Research Grants

  1. Inhibitors of 5alpha-reductase for acne therapy
    Lingna Li; Fiscal Year: 2002
  2. Orthotopic models of tumor angiogenesis and blood flow
    Meng Yang; Fiscal Year: 2007
  3. Therapeutic hair follicle-derived neurospheres
    Meng Yang; Fiscal Year: 2007
  4. Dual-color tumor-host imaging models
    Meng Yang; Fiscal Year: 2007
  5. Targeted tumoricidal bacteria
    Ming Zhao; Fiscal Year: 2007
  6. Orthotopic models of tumor angiogenesis and blood flow
    Meng Yang; Fiscal Year: 2006
  7. Dual-color tumor-host imaging models
    Meng Yang; Fiscal Year: 2006
  8. Imageable tumor-targeting bacteria
    Ming Zhao; Fiscal Year: 2006
  9. Enzyme therapy for hyperhomocysteinemia
    Yuying Tan; Fiscal Year: 2005
  10. Discovery of novel fluorescent reporter genes
    Ming Zhao; Fiscal Year: 2004

Collaborators

Detail Information

Publications138 found, 100 shown here

  1. ncbi request reprint Orthotopic metastatic (MetaMouse) models for discovery and development of novel chemotherapy
    Robert M Hoffman
    Department Surgery, University of California San Diego, and AntiCancer Inc, USA
    Methods Mol Med 111:297-322. 2005
    ..These SOI models are uniquely useful for innovative drug discovery and mechanism studies and serve as a bridge linking preclinical and clinical drug development...
  2. doi request reprint In vivo real-time imaging of nuclear-cytoplasmic dynamics of dormancy, proliferation and death of cancer cells
    Robert M Hoffman
    AntiCancer, Inc and Department of Surgery, University of California at San Diego, San Diego, CA 92111 3604, USA
    APMIS 116:716-29. 2008
    ..The implications of this technology for discovery of new visible targets at the cellular level for treatment of cancer are discussed...
  3. ncbi request reprint Dual-color imaging of nascent angiogenesis and its inhibition in liver metastases of pancreatic cancer
    Yasuyuki Amoh
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, California, USA
    Anticancer Res 26:3237-42. 2006
    ..This model will be useful for understanding the mechanism of angiogenesis of pancreatic cancer liver metastasis and for the discovery of effective new inhibitors of this process...
  4. ncbi request reprint Transgenic nude mice ubiquitously expressing fluorescent proteins for color-coded imaging of the tumor microenvironment
    Robert M Hoffman
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA, 92111, USA
    Methods Mol Biol 1194:353-65. 2014
    ..The GFP, RFP, and CFP nude mice when transplanted with cancer cells of another color are powerful models for color-coded imaging of the tumor microenvironment (TME) at the cellular level. ..
  5. ncbi request reprint Methods for the development of tumor-targeting bacteria
    Robert M Hoffman
    AntiCancer, Inc, San Diego, CA, USA
    Expert Opin Drug Discov 9:741-50. 2014
    ..There is also controversy about which bacteria are optimum for cancer treatment and whether bacteria should be used as tumor-targeting vectors, immune stimulators, or for direct tumor killing...
  6. pmc Development of a clinically-precise mouse model of rectal cancer
    Hiroyuki Kishimoto
    AntiCancer, Inc, San Diego, California, United States of America Department of Surgery, University of California San Diego, San Diego, California, United States of America Division of Surgical Oncology, Department of Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan
    PLoS ONE 8:e79453. 2013
    ..This rectal tumor model precisely mimics the natural history of rectal cancer and can be used to study early tumor development, metastasis, and discovery and evaluation of novel therapeutics for this treatment-resistant disease. ..
  7. ncbi request reprint Imaging nuclear - cytoplasm dynamics of cancer cells in the intravascular niche of live mice
    Atsushi Suetsugu
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111, U S A
    Anticancer Res 33:4229-36. 2013
    ..The subcellular in vivo imaging approach described in the present report provides new visual targets for trafficking and proliferating intravascular cancer cells as well as extravasating and invading cancer cells...
  8. ncbi request reprint High lung-metastatic variant of human osteosarcoma cells, selected by passage of lung metastasis in nude mice, is associated with increased expression of α(v)β(3) integrin
    Yasunori Tome
    AntiCancer Inc, 7917 Ostrow Street, San Diego, CA 92111, U S A
    Anticancer Res 33:3623-7. 2013
    ..With this highly metastatic variant overexpressing αvβ3 integrin, it will now be possible to further investigate the mechanism by which αvβ3 integrin facilitates metastasis. ..
  9. ncbi request reprint Real-time imaging of αv integrin molecular dynamics in osteosarcoma cells in vitro and in vivo
    Yasunori Tome
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111, USA
    Anticancer Res 33:3021-5. 2013
    ..These results show that αv integrin-GFP enables the imaging of the molecular dynamics of αv integrin in osteosarcoma cells in vitro and in vivo. ..
  10. ncbi request reprint Color-coded imaging of spontaneous vessel anastomosis in vivo
    Fuminari Uehara
    AntiCancer Inc, 7917 Ostrow Street, San Diego, California 92111, USA
    Anticancer Res 33:3041-5. 2013
    ..The results presented in this report indicate a possible mechanism for tumor angiogenesis and suggest a new paradigm of therapeutic revascularization of ischemic organs requiring new blood vessels and in other diseases. ..
  11. ncbi request reprint Salmonella typhimurium A1-R tumor targeting in immunocompetent mice is enhanced by a traditional Chinese medicine herbal mixture
    Yong Zhang
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111 USA
    Anticancer Res 33:1837-43. 2013
    ..The results of the present study suggest that the combination of A1-R and TCM has important potential for therapy of highly aggressive types of cancer, including those which are resistant to standard therapy...
  12. ncbi request reprint A color-coded imaging model of the interaction of αv integrin-GFP expressed in osteosarcoma cells and RFP expressing blood vessels in Gelfoam® vascularized in vivo
    Fuminari Uehara
    AntiCancer, Inc, San Diego, CA 92111, USA
    Anticancer Res 33:1361-6. 2013
    ..The observation of the behavior of αv integrin-GFP and blood vessels will allow further understanding of the role of αv integrin in cancer cells...
  13. ncbi request reprint Single cell time-lapse imaging of focus formation by the DNA damage-response protein 53BP1 after UVC irradiation of human pancreatic cancer cells
    Shinji Miwa
    AntiCancer, Inc, San Diego, CA 92111, USA
    Anticancer Res 33:1373-7. 2013
    ..Our results indicate that an early response to DNA damage caused by UVC irradiation can be visualized by increased GFP-53BP1 focus formation by pancreatic cancer cells...
  14. ncbi request reprint Dynamic subcellular imaging of cancer cell mitosis in the brain of live mice
    Masashi Momiyama
    AntiCancer, Inc, San Diego, CA 92111, USA
    Anticancer Res 33:1367-71. 2013
    ..Mitosis of the individual cancer cells were imaged dynamically in real time through the craniotomy-open window. This model can be used to evaluate brain metastasis and brain cancer at the subcellular level...
  15. ncbi request reprint Enhanced resection of orthotopic red-fluorescent-protein-expressing human glioma by fluorescence-guided surgery in nude mice
    Masashi Momiyama
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111, USA
    Anticancer Res 33:107-11. 2013
    ..These results suggest that fluorescence-guided surgery has significant potential for brain cancer treatment...
  16. ncbi request reprint Subcellular real-time imaging of the efficacy of temozolomide on cancer cells in the brain of live mice
    Masashi Momiyama
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111, USA
    Anticancer Res 33:103-6. 2013
    ..More effective drugs for brain cancer and brain metastasis can be screened and can be identified with this technology...
  17. ncbi request reprint Primer dosing of S. typhimurium A1-R potentiates tumor-targeting and efficacy in immunocompetent mice
    Yasunori Tome
    AntiCancer, Inc, 7917 Ostrow Street, SanDiego, CA 92111, USA
    Anticancer Res 33:97-102. 2013
    ..The results of the present study suggest effective future clinical strategies of bacterial treatment of cancer...
  18. ncbi request reprint Imaging the inhibition by anti-β1 integrin antibody of lung seeding of single osteosarcoma cells in live mice
    Hiroaki Kimura
    AntiCancer, Inc, San Diego, CA 92111, USA
    Int J Cancer 131:2027-33. 2012
    ..AIIB2 treatment also increased survival of mice with orthotopically growing 143B-RFP...
  19. ncbi request reprint Fluorescent proteins enhance UVC PDT of cancer cells
    Masashi Momiyama
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111, USA
    Anticancer Res 32:4327-30. 2012
    ..These results suggest that the expression of fluorescent proteins in cancer cells can enhance photodynamic therapy (PDT) using UVC and possibly with other wavelengths of light as well...
  20. pmc Bugging tumors
    Robert M Hoffman
    AntiCancer Inc, 7917 Ostrow Street, San Diego, CA 92111, USA
    Cancer Discov 2:588-90. 2012
    ..Because the barriers to efficacy of standard therapy of cancer appear to be opportunities for bacterial cancer therapy, the future of bacterial therapy of cancer appears bright...
  21. ncbi request reprint Non-invasive fluorescent-protein imaging of orthotopic pancreatic-cancer-patient tumorgraft progression in nude mice
    Atsushi Suetsugu
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111, USA
    Anticancer Res 32:3063-7. 2012
    ..This non-invasive imageable tumorgraft model will be valuable to screen for effective treatment options for individual patients with pancreatic cancer, as well as for the discovery of improved agents for this treatment-resistant disease...
  22. ncbi request reprint Orthotopic mouse models expressing fluorescent proteins for cancer drug discovery
    Robert M Hoffman
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111, USA 1 858 654 2555 1 858 268 4175
    Expert Opin Drug Discov 5:851-66. 2010
    ..Take home message: SOI mouse models of cancer reproduce the features of clinical cancer...
  23. ncbi request reprint Comparative chemosensitivity of circulating human prostate cancer cells and primary cancer cells
    Rhiana Menen
    AntiCancer Inc, 7917 Ostrow Street, San Diego, CA 92111, USA
    Anticancer Res 32:2881-4. 2012
    ..The future goal, based on these studies, is the culture of CTCs from cancer patients' peripheral blood for chemosensitivity testing, for improved individualized therapy...
  24. ncbi request reprint Determination of the optimal route of administration of Salmonella typhimurium A1-R to target breast cancer in nude mice
    Yong Zhang
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111, USA
    Anticancer Res 32:2501-8. 2012
    ..The results of this study suggest that p.o. administration was the most safe to the host and the i.v. route was most effective for tumor targeting with S. typhimurium A1-R...
  25. doi request reprint Subcellular imaging in vivo: the next GFP revolution
    Robert M Hoffman
    AntiCancer, Inc, Department of Surgery, University of California, San Diego, CA, USA
    Methods Mol Biol 872:255-63. 2012
    ..This technology has furthered our understanding of the spread of cancer at the subcellular level. Fluorescent proteins thereby provide the basis for the new field of in vivo cell biology...
  26. ncbi request reprint Inhibition of metastasis of circulating human prostate cancer cells in the chick embryo by an extracellular matrix produced by foreskin fibroblasts in culture
    Rhiana Menen
    AntiCancer Inc, 7917 Ostrow Street, San Diego, CA 92111, USA
    Anticancer Res 32:1573-7. 2012
    ..0001) and 4.18 in the CTC plus semi-solid ECM group (p<0.0001). The results demonstrate that reduction of CTC metastatic potential is possible, in this case with an ECM produced by human foreskin fibroblasts in culture...
  27. ncbi request reprint Imageable fluorescent metastasis resulting in transgenic GFP mice orthotopically implanted with human-patient primary pancreatic cancer specimens
    Atsushi Suetsugu
    Anti Cancer, Inc, San Diego, CA 92111, USA
    Anticancer Res 32:1175-80. 2012
    ..The new imageable model of patient pancreatic cancer metastasis provides unique opportunities to identify current and novel antimetastatic therapeutics for individual patients...
  28. doi request reprint The preclinical discovery of bacterial therapy for the treatment of metastatic cancer with unique advantages
    Robert M Hoffman
    AntiCancer, Inc, San Diego, CA 92111, USA
    Expert Opin Drug Discov 7:73-83. 2012
    ..Salmonella, on the other hand, are facultative aerobes that can grow aerobically or anaerobically and, therefore, grow on viable tumor tissue as well as necrotic tissue...
  29. ncbi request reprint Cellular and subcellular imaging in live mice using fluorescent proteins
    Robert M Hoffman
    AntiCancer, Inc, San Diego, CA 92111, USA
    Curr Pharm Biotechnol 13:537-44. 2012
    ..Such imaging possibilities can provide new visual targets for novel drug therapy. Fluorescent proteins thus enable both micro as well as macro imaging technology and thereby provide the basis for the new field of in vivo cell biology...
  30. pmc Efficacy against lung metastasis with a tumor-targeting mutant of Salmonella typhimurium in immunocompetent mice
    Ming Zhao
    AntiCancer, Inc, San Diego, CA, USA
    Cell Cycle 11:187-93. 2012
    ..The results in this report, demonstrating the anti-metastatic efficacy of S. typhimurium A1-R in immunocompetent mice, indicate the clinical potential of bacterial therapy of cancer...
  31. pmc Complementarity of ultrasound and fluorescence imaging in an orthotopic mouse model of pancreatic cancer
    Cynthia S Snyder
    Moores UCSD Cancer Center, University of California, La Jolla, CA, USA
    BMC Cancer 9:106. 2009
    ....
  32. ncbi request reprint Color-coded fluorescence imaging of tumor-host interactions
    Robert M Hoffman
    AntiCancer, 7917 Ostrow Street, San Diego, California 92111, USA
    Nat Protoc 1:928-35. 2006
    ..The 'color-coded' tumor-host models enable imaging and therefore a deeper understanding of the host cells involved and their function in tumor progression. Approximately 4-8 weeks are needed for these procedures...
  33. ncbi request reprint Subcellular imaging in the live mouse
    Robert M Hoffman
    AntiCancer Inc, 7917 Ostrow Street, San Diego, California 92111, USA
    Nat Protoc 1:775-82. 2006
    ..The double transfection and selection procedures described here take 6-8 weeks. Cancer cell trafficking, deformation, extravasation, mitosis and cell death can be imaged with clarity...
  34. ncbi request reprint The potential of nestin-expressing hair follicle stem cells in regenerative medicine
    Robert M Hoffman
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111, USA
    Expert Opin Biol Ther 7:289-91. 2007
    ..The transplanted mice regain the ability to walk normally. Thus, hair follicle stem cells provide an effective, accessible, autologous source of stem cells for treatment of peripheral nerve injury...
  35. ncbi request reprint Real-time subcellular imaging in live animals: new visible targets for cancer drug discovery
    Robert M Hoffman
    AntiCancer Inc, 7917 Ostrow Street, San Diego, CA 92111 3604, USA
    IDrugs 9:632-5. 2006
    ..Further developments in subcellular imaging in live animals is expected to result in a new 'in vivo cell biology' that will provide visible targets for cancer and other diseases...
  36. ncbi request reprint Targeted therapy with a Salmonella typhimurium leucine-arginine auxotroph cures orthotopic human breast tumors in nude mice
    Ming Zhao
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111, USA
    Cancer Res 66:7647-52. 2006
    ..These results suggest that amino acid auxotrophic virulent bacteria, which selectively infect and attack viable tumor tissue, are a promising approach to cancer therapy...
  37. ncbi request reprint The hair follicle and its stem cells as drug delivery targets
    Robert M Hoffman
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111, USA
    Expert Opin Drug Deliv 3:437-43. 2006
    ....
  38. ncbi request reprint The multiple uses of fluorescent proteins to visualize cancer in vivo
    Robert M Hoffman
    AntiCancer Inc and Department of Surgery, University of California, San Diego, 7917 Ostrow Street, San Diego, California 92111, USA
    Nat Rev Cancer 5:796-806. 2005
    ..Visualization of many aspects of cancer initiation and progression in vivo should be possible with fluorescent proteins...
  39. ncbi request reprint Whole-body imaging with fluorescent proteins
    Robert M Hoffman
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, California 92111, USA
    Nat Protoc 1:1429-38. 2006
    ..Fluorescent protein-based imaging technology can be used for whole-body imaging of fluorescent cells on essentially all organs. The timeline for these experiments varies from 2 days to 2 months...
  40. ncbi request reprint Whole-body imaging of bacterial infection and antibiotic response
    Robert M Hoffman
    AntiCancer Incorporated, 7917 Ostrow Street, San Diego, California, 92111, USA
    Nat Protoc 1:2988-94. 2006
    ..This imaging technology affords a powerful approach to visualizing the infection process, determining the tissue specificity of infection, the spatial migration of the infectious agents and the response to antimicrobial agents...
  41. ncbi request reprint Color-coded real-time subcellular fluorescence imaging of the interaction between cancer and host cells in live mice
    Kensuke Yamauchi
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, California 92111, USA
    Anticancer Res 32:39-43. 2012
    ..The results of this study show that differentially labeling cells with spectrally-distinct fluorescent protein can allow subcellular-resolution imaging of cell-cell interactions between host and cancer cells...
  42. ncbi request reprint The cyan fluorescent protein nude mouse as a host for multicolor-coded imaging models of primary and metastatic tumor microenvironments
    Atsushi Suetsugu
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111, USA
    Anticancer Res 32:31-8. 2012
    ..The multi-colored models described in this report provide new opportunities to study the cellular interactions in the live primary and metastatic TME...
  43. ncbi request reprint Comparison of cancer-cell seeding, viability and deformation in the lung, muscle and liver, visualized by subcellular real-time imaging in the live mouse
    Katsuhiro Hayashi
    AntiCancer Inc, 7917 Ostrow Street, San Diego, CA 92111, USA
    Anticancer Res 31:3665-72. 2011
    ..In each organ, single disseminated cells tended to die earlier than aggregated cells. The results of this study suggest that the early steps of metastasis are different in the lung, liver and muscle...
  44. doi request reprint Nestin-driven green fluorescent protein as an imaging marker for nascent blood vessels in mouse models of cancer
    Robert M Hoffman
    AntiCancer Inc and Department of Surgery, University of California, 7917, Ostrow Street, San Diego, CA 92111, USA
    Methods Mol Biol 689:183-204. 2011
    ..These fluorescent models can be used to study the early events of angiogenesis and to quantitatively determine efficacy of antiangiogenesis compounds...
  45. ncbi request reprint Broad selective efficacy of recombinant methioninase and polyethylene glycol-modified recombinant methioninase on cancer cells In Vitro
    Yuying Tan
    AntiCancer, Inc, San Diego, CA 92111, USA
    Anticancer Res 30:1041-6. 2010
    ..The results of the current study demonstrate the broad clinical potential for rMETase and PEG-rMETase for cancer treatment...
  46. ncbi request reprint Tumor-targeting amino acid auxotrophic Salmonella typhimurium
    Robert M Hoffman
    AntiCancer Inc, San Diego, CA 92111, USA
    Amino Acids 37:509-21. 2009
    ..Exploitation of the tumor-killing capability of Salmonella has great promise for a new paradigm of cancer therapy...
  47. doi request reprint Imaging cancer dynamics in vivo at the tumor and cellular level with fluorescent proteins
    Robert M Hoffman
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111 3604, USA
    Clin Exp Metastasis 26:345-55. 2009
    ..Fluorescent proteins thus enable both macro and micro imaging technology and thereby provide the basis for the new field of in vivo cell biology...
  48. ncbi request reprint A better fluorescent protein for whole-body imaging
    Robert M Hoffman
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111 3604, USA
    Trends Biotechnol 26:1-4. 2008
    ..It is the brightest known protein with emission at wavelengths longer than 620 nm. This new protein offers the potential for noninvasive whole-body imaging of numerous cellular and molecular processes in live animals...
  49. ncbi request reprint Advantages of multi-color fluorescent proteins for whole-body and in vivo cellular imaging
    Robert M Hoffman
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, California 92111, USA
    J Biomed Opt 10:41202. 2005
    ..These multicolored proteins have allowed the color-coding of cancer cells growing in vivo with distinction of different cell types, including host from tumor, with single-cell resolution...
  50. ncbi request reprint Imaging tumor angiogenesis with fluorescent proteins
    Robert M Hoffman
    AntiCancer, Inc San Diego, CA, USA
    APMIS 112:441-9. 2004
    ..Thus, fluorescent proteins expressed in vivo offer very high resolution and sensitivity for real-time imaging of angiogenesis...
  51. ncbi request reprint Green fluorescent protein imaging of tumor cells in mice
    Robert M Hoffman
    University of California San Diego, San Diego, CA 92111, USA
    Lab Anim (NY) 31:34-41. 2002
  52. ncbi request reprint Orthotopic transplant mouse models with green fluorescent protein-expressing cancer cells to visualize metastasis and angiogenesis
    R M Hoffman
    AntiCancer, Inc, San Diego, CA, USA
    Cancer Metastasis Rev 17:271-7. 1998
    ..The results with the GFP-transfected tumor cells, combined with the use of SOI, demonstrate a fundamental advance to visualize and study cancer metastasis and the role of angiogenesis and other factors in the metastatic process...
  53. ncbi request reprint In vivo imaging with fluorescent proteins: the new cell biology
    Robert M Hoffman
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111, USA
    Acta Histochem 106:77-87. 2004
    ....
  54. ncbi request reprint In vivo imaging of metastatic cancer with fluorescent proteins
    R M Hoffman
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111, USA
    Cell Death Differ 9:786-9. 2002
  55. ncbi request reprint Green fluorescent protein imaging of tumour growth, metastasis, and angiogenesis in mouse models
    Robert Hoffman
    AntiCancer Inc, San Diego, CA 92111, USA
    Lancet Oncol 3:546-56. 2002
    ..GFP technology has also been used for real-time imaging and quantification of angiogenesis...
  56. ncbi request reprint Topical liposome targeting of dyes, melanins, genes, and proteins selectively to hair follicles
    R M Hoffman
    AntiCancer, Inc, San Diego, CA 92111, USA
    J Drug Target 5:67-74. 1998
    ....
  57. ncbi request reprint Orthotopic metastatic mouse models for anticancer drug discovery and evaluation: a bridge to the clinic
    R M Hoffman
    AntiCancer, Inc, San Diego, CA 92111, USA
    Invest New Drugs 17:343-59. 1999
    ..These unique SOI models have been used for innovative drug discovery and mechanism studies and serve as a bridge linking pre-clinical and clinical research and drug development...
  58. ncbi request reprint Methioninase: a therapeutic for diseases related to altered methionine metabolism and transmethylation: cancer, heart disease, obesity, aging, and Parkinson's disease
    R M Hoffman
    AntiCancer, Incorporated, San Diego, CA 92111, USA
    Hum Cell 10:69-80. 1997
    ..Most importantly, we review and hypothesize how the developing therapeutic recombination methioninase (rMETase) can be utilized to cure or prevent all of these diseases...
  59. ncbi request reprint Determination of clonality of metastasis by cell-specific color-coded fluorescent-protein imaging
    Norio Yamamoto
    AntiCancer, Inc, San Diego, CA 92111, USA
    Cancer Res 63:7785-90. 2003
    ..The simple fluorescence method of determining clonality of metastases described here can allow large-scale clonal analysis in numerous types of metastatic models...
  60. ncbi request reprint Immune reactions in skin and hair follicle gene therapy
    Robert M Hoffman
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111, USA
    Mol Ther 7:294-5. 2003
  61. ncbi request reprint PEG-methioninase
    Robert M Hoffman
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111, USA
    Adv Exp Med Biol 519:69-79. 2003
  62. ncbi request reprint Methioninase gene therapy with selenomethionine induces apoptosis in bcl-2-overproducing lung cancer cells
    Norio Yamamoto
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111, USA
    Cancer Gene Ther 10:445-50. 2003
    ..rAdMET/SeMET prodrug gene therapy is therefore a promising novel strategy effective against bcl-2 overexpression, which has blocked other gene therapy strategies...
  63. ncbi request reprint Visualization of GFP-expressing tumors and metastasis in vivo
    R M Hoffman
    AntiCancer, Inc, San Diego, CA, USA
    Biotechniques 30:1016-22, 1024-6. 2001
    ..The GFP-transfected tumor cells enabled a fundamental advance in the visualization of tumor growth and metastasis in real time in vivo...
  64. doi request reprint Tumor-seeking Salmonella amino acid auxotrophs
    Robert M Hoffman
    Department of Surgery, University of California, San Diego, CA 92111, USA
    Curr Opin Biotechnol 22:917-23. 2011
    ..The approach described here is a significant improvement over previous bacterial tumor-therapy strategies that require combination with toxic chemotherapy...
  65. ncbi request reprint Combination efficacy of doxorubicin and adenoviral methioninase gene therapy with prodrug selenomethionine
    Anshu Gupta
    AntiCancer, Inc, Department of Surgery, University of California at San Diego, San Diego, CA, USA
    Anticancer Res 23:1181-8. 2003
    ..The tumor doubling-time was increased to approximately 10 days with the combination therapy of Ad-MET + SeMET + DOX as opposed to 2-3 days in all other treatment groups...
  66. ncbi request reprint Targeted therapy of spinal cord glioma with a genetically modified Salmonella typhimurium
    H Kimura
    AntiCancer, Inc, San Diego, CA, USA
    Cell Prolif 43:41-8. 2010
    ..In this study, we measured antitumour efficacy of the Salmonella typhimurium A1-R tumour-targeting bacterium strain, administered systemically or intrathecally, to spinal cord cancer in orthotopic mouse models...
  67. pmc Whole-body and intravital optical imaging of angiogenesis in orthotopically implanted tumors
    M Yang
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111, USA
    Proc Natl Acad Sci U S A 98:2616-21. 2001
    ..These powerful and clinically relevant angiogenesis mouse models can be used for real-time in vivo evaluation of agents inhibiting or promoting tumor angiogenesis in physiological microenvironments...
  68. ncbi request reprint Nestin-linked green fluorescent protein transgenic nude mouse for imaging human tumor angiogenesis
    Yasuyuki Amoh
    AntiCancer, Inc, San Diego, California 92111, USA
    Cancer Res 65:5352-7. 2005
    ..These results suggest that this model is useful for the imaging of the angiogenesis of human as well as rodent tumors and visualization of the efficacy of angiogenetic inhibitors...
  69. ncbi request reprint Optically imageable metastatic model of human breast cancer
    Xiaoming Li
    AntiCancer, Inc, San Diego, California 92111, USA
    Clin Exp Metastasis 19:347-50. 2002
    ..The metastatic pattern in the model thus reflected clinical metastatic breast cancer and provides a powerful model for drug discovery for this disease...
  70. pmc Dual-color imaging of angiogenesis and its inhibition in bone and soft tissue sarcoma
    Katsuhiro Hayashi
    Department of Surgery, University of California San Diego, San Diego, California, USA
    J Surg Res 140:165-70. 2007
    ..We demonstrate in this report, using powerful color-coded fluorescent imageable tumor-host models, the onset of angiogenesis of these sarcomas and its inhibition...
  71. ncbi request reprint Cellular dynamics visualized in live cells in vitro and in vivo by differential dual-color nuclear-cytoplasmic fluorescent-protein expression
    Norio Yamamoto
    AntiCancer, Inc, San Diego, California
    Cancer Res 64:4251-6. 2004
    ..The dual-color cells thus are a useful tool for visualizing living-cell dynamics in vivo as well as in vitro. Drugs that could specifically perturb these processes can now be readily screened in real time in vivo...
  72. ncbi request reprint Real-time imaging of individual fluorescent-protein color-coded metastatic colonies in vivo
    Norio Yamamoto
    AntiCancer, Inc, San Diego, California 92111, USA
    Clin Exp Metastasis 20:633-8. 2003
    ..The simultaneous real-time dual-color imaging of metastatic colonies described in this report gives rise to the possibility of color-coded imaging of clones of cancer cells carrying various forms of gene of interest...
  73. ncbi request reprint Monitoring of skeletal progression of prostate cancer by GFP imaging, X-ray, and serum OPG and PTHrP
    D W Burton
    Department of Medicine, University of California and San Diego Veterans Administration Healthcare System VASDHS, San Diego, California 92161, USA
    Prostate 62:275-81. 2005
    ..We exploited both concepts by developing a GFP-expressing PCa cell line that expresses PTHrP and studying it in an animal model of malignancy with methods that assess the skeletal progression of this tumor...
  74. ncbi request reprint Methioninase cancer gene therapy with selenomethionine as suicide prodrug substrate
    K Miki
    AntiCancer Incorporated, San Diego, California 92111, USA
    Cancer Res 61:6805-10. 2001
    ..Adenoviral MET-gene/SeMET treatment also inhibited tumor growth in rodents and significantly prolonged their survival. Recombinant adenovirus-encoding MET gene-SeMET treatment thereby offers a new paradigm for cancer gene therapy...
  75. ncbi request reprint Transgenic nude mouse with ubiquitous green fluorescent protein expression as a host for human tumors
    Meng Yang
    AntiCancer, Inc, San Diego, California 92111, USA
    Cancer Res 64:8651-6. 2004
    ..The GFP mouse model should greatly expand our knowledge of human tumor-host interaction...
  76. ncbi request reprint The camptothecin derivative CPT-11 inhibits angiogenesis in a dual-color imageable orthotopic metastatic nude mouse model of human colon cancer
    Yong Ji
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111, USA
    Anticancer Res 27:713-8. 2007
    ..The results showed that CPT-11 is an effective inhibitor of angiogenesis and provided strong implications for wider clinical application of CPT-11 for colon cancer...
  77. ncbi request reprint Dual-color imaging of nascent blood vessels vascularizing pancreatic cancer in an orthotopic model demonstrates antiangiogenesis efficacy of gemcitabine
    Yasuyuki Amoh
    Department of Surgery, University of California San Diego, San Diego, California, USA
    J Surg Res 132:164-9. 2006
    ..The stem cell marker nestin recently has been shown to be expressed in nascent blood vessels in nestin-driven green fluorescent protein (ND-GFP) transgenic nude mice...
  78. pmc Monotherapy with a tumor-targeting mutant of Salmonella typhimurium cures orthotopic metastatic mouse models of human prostate cancer
    Ming Zhao
    AntiCancer Inc, 7917 Ostrow Street, San Diego, CA 92111, USA
    Proc Natl Acad Sci U S A 104:10170-4. 2007
    ..The approach described here, where bacterial monotherapy effectively treats metastatic prostate tumors, is a significant improvement over previous bacterial tumor-therapy strategies that require combination with toxic chemotherapy...
  79. pmc Spatial-temporal imaging of bacterial infection and antibiotic response in intact animals
    M Zhao
    AntiCancer, Inc, San Diego, CA 92111, USA
    Proc Natl Acad Sci U S A 98:9814-8. 2001
    ..This imaging technology affords a powerful approach to visualizing the infection process, determining the tissue specificity of infection, and the spatial migration of the infectious agents...
  80. doi request reprint A color-coded orthotopic nude-mouse treatment model of brain-metastatic paralyzing spinal cord cancer that induces angiogenesis and neurogenesis
    K Hayashi
    AntiCancer, Inc, San Diego, CA 92111, USA
    Cell Prolif 42:75-82. 2009
    ..Cancer of the spinal cord is highly malignant and often leads to paralysis and death. A realistic mouse model would be an important benefit for the better understanding and treatment of spinal cord glioma...
  81. ncbi request reprint Methionine depletion modulates the antitumor and antimetastatic efficacy of ethionine
    H Guo
    AntiCancer, Inc, San Diego, CA 92111, USA
    Anticancer Res 16:2719-23. 1996
    ....
  82. ncbi request reprint Development of real-time subcellular dynamic multicolor imaging of cancer-cell trafficking in live mice with a variable-magnification whole-mouse imaging system
    Kensuke Yamauchi
    AntiCancer, Inc, San Diego, California 92111, USA
    Cancer Res 66:4208-14. 2006
    ..This imaging technology will enable further understanding of the critical steps of metastasis and provide visible targets for antimetastasis drug development...
  83. pmc Dual-color fluorescence imaging distinguishes tumor cells from induced host angiogenic vessels and stromal cells
    Meng Yang
    AntiCancer, 7917 Ostrow Street, San Diego, CA 92111, USA
    Proc Natl Acad Sci U S A 100:14259-62. 2003
    ..Dual-color fluorescence imaging visualizes the tumor-host interaction by whole-body imaging and at the cellular level in fresh tissues, dramatically expanding previous studies in fixed and stained preparations...
  84. pmc Nestin expression in hair follicle sheath progenitor cells
    Lingna Li
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111, USA
    Proc Natl Acad Sci U S A 100:9958-61. 2003
    ..The expression of the unique protein, nestin, in both neural stem cells and hair follicle stem cells suggests their possible relation...
  85. ncbi request reprint Whole-body subcellular multicolor imaging of tumor-host interaction and drug response in real time
    Meng Yang
    AntiCancer, Inc, San Diego, California 92111, USA
    Cancer Res 67:5195-200. 2007
    ..This imageable model should lead to many new insights of in vivo cancer cell biology and to novel drug discovery...
  86. ncbi request reprint Efficacy of intra-hepatectomy 5-FU on recurrence and metastasis of human hepatocellular carcinoma in nude mice
    B Rashidi
    AntiCancer, Inc, San Diego, CA, USA
    Int J Cancer 91:231-5. 2001
    ..The results of this study indicate that IHC therapy for resection of HCC should be investigated clinically...
  87. doi request reprint Real-time imaging of single cancer-cell dynamics of lung metastasis
    Hiroaki Kimura
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, California 92111, USA
    J Cell Biochem 109:58-64. 2010
    ..This new technology makes it possible to observe real-time monitoring of cancer-cell dynamics of metastasis in the lung and to identify potential metastatic stem cells...
  88. ncbi request reprint Real-time optical imaging of primary tumor growth and multiple metastatic events in a pancreatic cancer orthotopic model
    Michael Bouvet
    Department of Surgery, University of California San Diego, San Diego, California 92161, USA
    Cancer Res 62:1534-40. 2002
    ..The GFP imaging technology presented in this report will facilitate studies of modulators of pancreatic cancer growth, including inhibition by potential chemotherapeutic agents...
  89. pmc Fluorescent LYVE-1 antibody to image dynamically lymphatic trafficking of cancer cells in vivo
    Michele McElroy
    Department of Surgery, University of California, San Diego, California, USA
    J Surg Res 151:68-73. 2009
    ..Despite ongoing interest in this area of research, the real-time behavior of cancer cells trafficking in the lymphatic system is poorly understood due to lack of appropriate tools to image this process...
  90. pmc Tumor-targeting bacterial therapy with amino acid auxotrophs of GFP-expressing Salmonella typhimurium
    Ming Zhao
    AntiCancer, 7917 Ostrow Street, San Diego, CA 92111 3604, USA
    Proc Natl Acad Sci U S A 102:755-60. 2005
    ..This result is in marked contrast to bacteria previously tried for cancer therapy that were confined to necrotic areas of the tumor, which may account, in part, for the strain's unique antitumor efficacy...
  91. ncbi request reprint Real-time GFP imaging of spontaneous HT-1080 fibrosarcoma lung metastases
    Norio Yamamoto
    AntiCancer, Inc, San Diego, California 92111, USA
    Clin Exp Metastasis 20:181-5. 2003
    ..This procedure enabled external imaging at the micrometastasis level. Real-time evaluation of spontaneous lung metastasis in the same animals should allow drug evaluation and mechanistic studies not previously possible...
  92. ncbi request reprint Color coding cancer cells with fluorescent proteins to visualize in vivo cellular interaction in metastatic colonies
    Norio Yamamoto
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111, USA
    Anticancer Res 24:4067-72. 2004
    ..Fluorescence color coding of cancer cells enables visualization of the interaction of cancer cells and can be used to distinguish cancer cells of any genotype or phenotype...
  93. pmc High efficiency genetic modification of hair follicles and growing hair shafts
    Norimitsu Saito
    AntiCancer, Inc, San Diego, CA 92111, USA
    Proc Natl Acad Sci U S A 99:13120-4. 2002
    ..Most follicles produced GFP-fluorescent growing hair shafts. This technique has produced efficient genetic modification of the hair shaft...
  94. doi request reprint Isolation and culture of hair follicle pluripotent stem (hfPS) cells and their use for nerve and spinal cord regeneration
    Yasuyuki Amoh
    AntiCancer, Inc, San Diego, CA, USA
    Methods Mol Biol 585:401-20. 2010
    ..We have termed these cells hair follicle pluripotent stem (hfPS) cells. These results suggest that hfPS cells provide an important accessible, autologous source of adult stem cells with potential for use in regenerative medicine...
  95. ncbi request reprint In vivo color-coded imaging of the interaction of colon cancer cells and splenocytes in the formation of liver metastases
    Michael Bouvet
    Department of Surgery, University of California, San Diego, USA
    Cancer Res 66:11293-7. 2006
    ..These results suggest a novel tumor-host interaction that enables efficient formation of liver metastasis via intrasplenic injection...
  96. pmc Cancer metastasis directly eradicated by targeted therapy with a modified Salmonella typhimurium
    Katsuhiro Hayashi
    AntiCancer, Inc, 7917 Ostrow Street, San Diego, CA 92111, USA
    J Cell Biochem 106:992-8. 2009
    ..No adverse effects were observed. This new strategy demonstrates the clinical potential of targeting and curing cancer metastasis with engineered bacteria without the need of toxic chemotherapy...
  97. ncbi request reprint Real-time in vivo dual-color imaging of intracapillary cancer cell and nucleus deformation and migration
    Kensuke Yamauchi
    AntiCancer, Inc, San Diego, California 92111, USA
    Cancer Res 65:4246-52. 2005
    ..The use of the dual-color cancer cells differentially labeled in the cytoplasm and nucleus and associated fluorescent imaging provide a powerful tool to understand the mechanism of cancer cell migration and deformation in small vessels...
  98. ncbi request reprint Metastatic orthotopic mouse models of lung cancer
    Robert M Hoffman
    AntiCancer Inc, San Diego, CA, USA
    Methods Mol Med 74:457-64. 2003
  99. pmc In vivo internal tumor illumination by telomerase-dependent adenoviral GFP for precise surgical navigation
    Hiroyuki Kishimoto
    AntiCancer, Inc, San Diego, CA 92111, USA
    Proc Natl Acad Sci U S A 106:14514-7. 2009
    ..The data in this report suggest that adenoviral-GFP labeling tumors in patients can enable fluorescence-guided surgical navigation...

Research Grants12

  1. Inhibitors of 5alpha-reductase for acne therapy
    Lingna Li; Fiscal Year: 2002
    ..PROPOSED COMMERCIAL APPLICATIONS: Liposomal 4-MA will be developed as a topical selectively targeted therapeutic for acne for which they should be a very market. ..
  2. Orthotopic models of tumor angiogenesis and blood flow
    Meng Yang; Fiscal Year: 2007
    ....
  3. Therapeutic hair follicle-derived neurospheres
    Meng Yang; Fiscal Year: 2007
    ..Human hair- follicle bulge cells will be further characterized and developed for therapeutic potential for nerve regeneration in Phase III. ..
  4. Dual-color tumor-host imaging models
    Meng Yang; Fiscal Year: 2007
    ..These models have significant commercial potential for discovery and development of stroma-targeted and anti-angiogenesis drugs. ..
  5. Targeted tumoricidal bacteria
    Ming Zhao; Fiscal Year: 2007
    ..Future human trials of the tumor-killing bacteria can be held after the Phase I and Phase II grant periods are completed. ..
  6. Orthotopic models of tumor angiogenesis and blood flow
    Meng Yang; Fiscal Year: 2006
    ....
  7. Dual-color tumor-host imaging models
    Meng Yang; Fiscal Year: 2006
    ..These models have significant commercial potential for discovery and development of stroma-targeted and anti-angiogenesis drugs. ..
  8. Imageable tumor-targeting bacteria
    Ming Zhao; Fiscal Year: 2006
    ..In addition, Salmonella amino acid tumor-targeting auxotroph strains will be modified to secrete cytokines to enhance the bacterial killing of the tumor with stimulation of host antitumor immune reactions in Phase II. ..
  9. Enzyme therapy for hyperhomocysteinemia
    Yuying Tan; Fiscal Year: 2005
    ..Phase II results will lead to an IND for MEGC-PEG-rMETase for end stage renal disease patients and other patients with intractable hyperhomocysteinemia and high cardiovascular disease mortality. ..
  10. Discovery of novel fluorescent reporter genes
    Ming Zhao; Fiscal Year: 2004
    ..Reporters with spectral properties that can be used for whole-body imaging of tumors in the lung and their metastases will be candidates for further development for numerous applications of multi-color imaging. ..
  11. RHOMOCYCSTEINASE FOR HOMOCYSTEINE ASSAY
    Yuying Tan; Fiscal Year: 2002
    ..The tHCY enzymatic kits for these applications will be ready for commercial launch at this point. PROPOSED COMMERCIAL APPLICATION: Not Available ..