Xin Huang

Summary

Affiliation: Amgen Inc
Country: USA

Publications

  1. ncbi request reprint Crystal structure of an inactive Akt2 kinase domain
    Xin Huang
    Amgen Cambridge Research Center, One Kendall Square, Building 1000, Cambridge, MA 02139, USA
    Structure 11:21-30. 2003
  2. doi request reprint Discovery of novel, induced-pocket binding oxazolidinones as potent, selective, and orally bioavailable tankyrase inhibitors
    Howard Bregman
    Department of Chemistry Research and Discovery, Amgen Inc, 360 Binney Street, Cambridge, Massachusetts 02142, USA
    J Med Chem 56:4320-42. 2013
  3. doi request reprint Discovery of a class of novel tankyrase inhibitors that bind to both the nicotinamide pocket and the induced pocket
    Howard Bregman
    Department of Medicinal Chemistry, Amgen Inc, 360 Binney Street, Cambridge, Massachusetts 02142, USA
    J Med Chem 56:1341-5. 2013
  4. ncbi request reprint Discovery of 4-amino-5,6-biaryl-furo[2,3-d]pyrimidines as inhibitors of Lck: development of an expedient and divergent synthetic route and preliminary SAR
    Erin F DiMauro
    Department of Medicinal Chemistry, Amgen Inc, One Kendall Square, Building 1000, Cambridge, MA 02139, USA
    Bioorg Med Chem Lett 17:2305-9. 2007
  5. doi request reprint Pyridyl-pyrimidine benzimidazole derivatives as potent, selective, and orally bioavailable inhibitors of Tie-2 kinase
    Victor J Cee
    Department of Medicinal Chemistry, Amgen Inc, One Kendall Square, Bldg 1000, Cambridge, MA 02139, USA
    Bioorg Med Chem Lett 19:424-7. 2009
  6. doi request reprint Structure-guided design of aminopyrimidine amides as potent, selective inhibitors of lymphocyte specific kinase: synthesis, structure-activity relationships, and inhibition of in vivo T cell activation
    Erin F DiMauro
    Department of Medicinal Chemistry, Amgen Inc, One Kendall Square, Cambridge, MA 02139, USA
    J Med Chem 51:1681-94. 2008
  7. doi request reprint Structure-based design of novel 2-amino-6-phenyl-pyrimido[5',4':5,6]pyrimido[1,2-a]benzimidazol-5(6H)-ones as potent and orally active inhibitors of lymphocyte specific kinase (Lck): synthesis, SAR, and in vivo anti-inflammatory activity
    Matthew W Martin
    Department of Medicinal Chemistry, Amgen Inc, One Kendall Square, Cambridge, MA 02139, USA
    J Med Chem 51:1637-48. 2008
  8. ncbi request reprint Discovery of novel 2,3-diarylfuro[2,3-b]pyridin-4-amines as potent and selective inhibitors of Lck: synthesis, SAR, and pharmacokinetic properties
    Matthew W Martin
    Department of Medicinal Chemistry, Amgen Inc, One Kendall Square, Building 1000, Cambridge, MA 02139, USA
    Bioorg Med Chem Lett 17:2299-304. 2007
  9. ncbi request reprint N-(3-(phenylcarbamoyl)arylpyrimidine)-5-carboxamides as potent and selective inhibitors of Lck: structure, synthesis and SAR
    Holly L Deak
    Department of Medicinal Chemistry, Amgen Inc, One Kendall Square, Building 1000, Cambridge, MA 02139, USA
    Bioorg Med Chem Lett 18:1172-6. 2008
  10. ncbi request reprint Discovery of aminoquinazolines as potent, orally bioavailable inhibitors of Lck: synthesis, SAR, and in vivo anti-inflammatory activity
    Erin F DiMauro
    Department of Medicinal Chemistry, Amgen, Inc, Cambridge, Massachusetts 02139, USA
    J Med Chem 49:5671-86. 2006

Detail Information

Publications65

  1. ncbi request reprint Crystal structure of an inactive Akt2 kinase domain
    Xin Huang
    Amgen Cambridge Research Center, One Kendall Square, Building 1000, Cambridge, MA 02139, USA
    Structure 11:21-30. 2003
    ..Residues within the linker region between the N- and C-terminal lobes also contribute to the inactive conformation by partially occupying the ATP binding site...
  2. doi request reprint Discovery of novel, induced-pocket binding oxazolidinones as potent, selective, and orally bioavailable tankyrase inhibitors
    Howard Bregman
    Department of Chemistry Research and Discovery, Amgen Inc, 360 Binney Street, Cambridge, Massachusetts 02142, USA
    J Med Chem 56:4320-42. 2013
    ..Herein, we describe the utilization of structure-based design and molecular modeling toward novel, potent, and selective tankyrase inhibitors with improved pharmacokinetic properties (39, 40)...
  3. doi request reprint Discovery of a class of novel tankyrase inhibitors that bind to both the nicotinamide pocket and the induced pocket
    Howard Bregman
    Department of Medicinal Chemistry, Amgen Inc, 360 Binney Street, Cambridge, Massachusetts 02142, USA
    J Med Chem 56:1341-5. 2013
    ..The crystal structure of human TNKS1 in complex with this "dual-binder" provides a molecular basis for their strong and specific interactions and suggests clues for the further development of tankyrase inhibitors...
  4. ncbi request reprint Discovery of 4-amino-5,6-biaryl-furo[2,3-d]pyrimidines as inhibitors of Lck: development of an expedient and divergent synthetic route and preliminary SAR
    Erin F DiMauro
    Department of Medicinal Chemistry, Amgen Inc, One Kendall Square, Building 1000, Cambridge, MA 02139, USA
    Bioorg Med Chem Lett 17:2305-9. 2007
    ..Lead optimization was guided by X-ray crystallographic data, and preliminary SAR led to the identification of compounds with improved cellular potency and selectivity...
  5. doi request reprint Pyridyl-pyrimidine benzimidazole derivatives as potent, selective, and orally bioavailable inhibitors of Tie-2 kinase
    Victor J Cee
    Department of Medicinal Chemistry, Amgen Inc, One Kendall Square, Bldg 1000, Cambridge, MA 02139, USA
    Bioorg Med Chem Lett 19:424-7. 2009
    ..Reported herein is the optimization of a nonselective scaffold into a potent and highly selective inhibitor of Tie-2 kinase...
  6. doi request reprint Structure-guided design of aminopyrimidine amides as potent, selective inhibitors of lymphocyte specific kinase: synthesis, structure-activity relationships, and inhibition of in vivo T cell activation
    Erin F DiMauro
    Department of Medicinal Chemistry, Amgen Inc, One Kendall Square, Cambridge, MA 02139, USA
    J Med Chem 51:1681-94. 2008
    ..Orally bioavailable compound 13b inhibited the anti-CD3-induced production of interleukin-2 (IL-2) in mice in a dose-dependent manner (ED 50 = 9.4 mg/kg)...
  7. doi request reprint Structure-based design of novel 2-amino-6-phenyl-pyrimido[5',4':5,6]pyrimido[1,2-a]benzimidazol-5(6H)-ones as potent and orally active inhibitors of lymphocyte specific kinase (Lck): synthesis, SAR, and in vivo anti-inflammatory activity
    Matthew W Martin
    Department of Medicinal Chemistry, Amgen Inc, One Kendall Square, Cambridge, MA 02139, USA
    J Med Chem 51:1637-48. 2008
    ..This activity translates into inhibition of in vitro cell-based assays and in vivo models of T-cell activation and arthritis, respectively...
  8. ncbi request reprint Discovery of novel 2,3-diarylfuro[2,3-b]pyridin-4-amines as potent and selective inhibitors of Lck: synthesis, SAR, and pharmacokinetic properties
    Matthew W Martin
    Department of Medicinal Chemistry, Amgen Inc, One Kendall Square, Building 1000, Cambridge, MA 02139, USA
    Bioorg Med Chem Lett 17:2299-304. 2007
    ..The discovery, synthesis, and structure activity relationships of this series of inhibitors are reported. The most promising compounds were also profiled to deduce their pharmacokinetic properties...
  9. ncbi request reprint N-(3-(phenylcarbamoyl)arylpyrimidine)-5-carboxamides as potent and selective inhibitors of Lck: structure, synthesis and SAR
    Holly L Deak
    Department of Medicinal Chemistry, Amgen Inc, One Kendall Square, Building 1000, Cambridge, MA 02139, USA
    Bioorg Med Chem Lett 18:1172-6. 2008
    ..X-ray co-crystal structural data, structure-activity relationships, and the synthesis of these inhibitors are reported herein...
  10. ncbi request reprint Discovery of aminoquinazolines as potent, orally bioavailable inhibitors of Lck: synthesis, SAR, and in vivo anti-inflammatory activity
    Erin F DiMauro
    Department of Medicinal Chemistry, Amgen, Inc, Cambridge, Massachusetts 02139, USA
    J Med Chem 49:5671-86. 2006
    ..Optimized, orally bioavailable compounds 32 and 47 exhibit anti-inflammatory activity (ED(50) of 22 and 11 mg/kg, respectively) in the anti-CD3-induced production of interleukin-2 (IL-2) in mice...
  11. pmc A general joint model for longitudinal measurements and competing risks survival data with heterogeneous random effects
    Xin Huang
    Amgen Inc, 1120 Veterans Boulevard, Mail Stop ASF3 3, South San Francisco, CA 94080, USA
    Lifetime Data Anal 17:80-100. 2011
    ..A Bayesian MCMC procedure is developed for parameter estimation and inference. Its performances and frequentist properties are investigated using simulations. A real data example is used to illustrate the usefulness of the approach...
  12. doi request reprint Discovery of a potent, selective, and orally bioavailable pyridinyl-pyrimidine phthalazine aurora kinase inhibitor
    Victor J Cee
    Department of Medicinal Chemistry, Amgen Inc, 360 Binney Street, Cambridge, Massachusetts 02142 and Amgen Inc, One Amgen Center Drive, Thousand Oaks, California 91320, USA
    J Med Chem 53:6368-77. 2010
    ....
  13. ncbi request reprint Novel 2-aminopyrimidine carbamates as potent and orally active inhibitors of Lck: synthesis, SAR, and in vivo antiinflammatory activity
    Matthew W Martin
    Department of Chemistry Research and Discovery, Amgen Inc, One Kendall Square, Building 1000, Cambridge, Massachusetts 02139, USA
    J Med Chem 49:4981-91. 2006
    ....
  14. doi request reprint Discovery and optimization of chromenotriazolopyrimidines as potent inhibitors of the mouse double minute 2-tumor protein 53 protein-protein interaction
    John G Allen
    Chemistry Research and Discovery, Amgen Inc, One Amgen Center Drive, Thousand Oaks, California 91320, USA
    J Med Chem 52:7044-53. 2009
    ..In cellular assays, these compounds were shown to upregulate p53 protein levels and p53 signaling and to cause p53-dependent inhibition of proliferation and apoptosis...
  15. doi request reprint 2-Phenylamino-6-cyano-1H-benzimidazole-based isoform selective casein kinase 1 gamma (CK1γ) inhibitors
    Zihao Hua
    Medicinal Chemistry, Amgen Inc, 360 Binney Street, Cambridge, MA 02142, USA
    Bioorg Med Chem Lett 22:5392-5. 2012
    ..Further structure-based optimization of this series resulted in the discovery of 1h which possessed good enzymatic and cellular potency, excellent CK1 isoform and kinase selectivity, and acceptable pharmacokinetic properties...
  16. doi request reprint Structural basis for the potent and selective inhibition of casein kinase 1 epsilon
    Alexander M Long
    Department of Molecular Structure and Characterization, Amgen Inc, 360 Binney Street, Cambridge, Massachusetts 02142, USA
    J Med Chem 55:10307-11. 2012
    ..These structures provide a molecular basis for the strong and specific inhibitor interactions with CK1ε and suggest clues for further development of CK1δ inhibitors...
  17. doi request reprint Discovery of potent and highly selective thienopyridine Janus kinase 2 inhibitors
    Laurie B Schenkel
    Department of Medicinal Chemistry, Amgen, Inc, 360 Binney Street, Cambridge, Massachusetts 02142, USA
    J Med Chem 54:8440-50. 2011
    ....
  18. doi request reprint Discovery of 2,4-bis-arylamino-1,3-pyrimidines as insulin-like growth factor-1 receptor (IGF-1R) inhibitors
    John L Buchanan
    Department of Medicinal Chemistry, Amgen Inc, 360 Binney Street, Cambridge, MA 02142, USA
    Bioorg Med Chem Lett 21:2394-9. 2011
    ..Herein, we report the synthesis, structure-activity relationships (SAR), X-ray cocrystal structure and in vivo tumor study results for a series of 2,4-bis-arylamino-1,3-pyrimidines...
  19. ncbi request reprint Self-assembled spiral nanoarchitecture and supramolecular chirality in Langmuir-Blodgett films of an achiral amphiphilic barbituric acid
    Xin Huang
    CAS Key Laboratory of Colloid and Interface Science, Institute of Chemistry, CAS, 100080 Beijing, PR China
    J Am Chem Soc 126:1322-3. 2004
    ..To the best of our knowledge, this is the first report on the chirality of the molecular assemblies and spiral nanostructures formed through the air/water interface by achiral molecules...
  20. ncbi request reprint Interconversion of ATP binding and conformational free energies by tryptophanyl-tRNA synthetase: structures of ATP bound to open and closed, pre-transition-state conformations
    Pascal Retailleau
    Department of Biochemistry and Biophysics, University of North Carolina, Mary Ellen Jones Bldg CB 7260, Chapel Hill, NC 27599 7260, USA
    J Mol Biol 325:39-63. 2003
    ..The paradoxical requirement for high [ATP] implies that Gibbs binding free energy is stored in an unfavorable protein conformation and can then be recovered for useful purposes, including catalysis in the case of TrpRS...
  21. ncbi request reprint A set of genes selectively expressed in murine dendritic cells: utility of related cis-acting sequences for lentiviral gene transfer
    Kevin S Gorski
    Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Mol Immunol 40:35-47. 2003
    ..Endogenous CCL17 and B7-DC mRNAs were increased similarly in IL-4 cultured DCs but only CCL17 was induced by LPS. Additionally, IL-4 increased cell surface expression of B7-DC in both immature and mature DCs...
  22. ncbi request reprint Phase I clinical and pharmacodynamic evaluation of oral CI-1033 in patients with refractory cancer
    Ralph G Zinner
    M D Anderson Cancer Center, Houston, Texas, USA
    Clin Cancer Res 13:3006-14. 2007
    ..Plasma biomarkers HER2, vascular endothelial growth factor, interleukin-8, and matrix metalloproteinase-9 were evaluated using immunologic techniques...
  23. ncbi request reprint Loss of distal 11q is associated with DNA repair deficiency and reduced sensitivity to ionizing radiation in head and neck squamous cell carcinoma
    Rahul A Parikh
    Department of Human Genetics, University of Pittsburgh Graduate School of Public Health, Pittsburgh, PA 15261, USA
    Genes Chromosomes Cancer 46:761-75. 2007
    ..Although the literature attributes the poor prognosis in HNSCC to 11q13 gene amplification, our results suggest that distal 11q deletions may be an equally significant factor...
  24. ncbi request reprint A novel anticancer agent, icaritin, induced cell growth inhibition, G1 arrest and mitochondrial transmembrane potential drop in human prostate carcinoma PC-3 cells
    Xin Huang
    Institute of Pharmacology and Toxicology and Biochemical Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, 353 Yan An Road, Hangzhou 310031, China
    Eur J Pharmacol 564:26-36. 2007
    ..These findings suggested a novel anticancer efficacy of icaritin mediated selectively via induction of cell cycle arrest but not associated with estrogen receptors in PC-3 cells...
  25. ncbi request reprint Self-assembled nanowire networks of aryloxy zinc phthalocyanines based on Zn-O coordination
    Xin Huang
    Department of Chemistry, Tsinghua University, Beijing 100084, People s Republic of China
    Langmuir 23:5167-72. 2007
    ..The driving force for the aggregate formation is proposed to be the complementary coordination of the ether oxygen in the aryloxy groups of one molecule to the core Zn of another molecule of phthalocyanine...
  26. ncbi request reprint In vitro bioactivity and osteoblast-like cell test of zinc containing fluoridated hydroxyapatite films
    Shundong Miao
    Department of Materials Science and Engineering, Zhejiang University, Hangzhou 310009, China
    J Mater Sci Mater Med 18:2101-5. 2007
    ..The results in this work suggest that ZnFHA films on Ti6Al4V substrates can function as an implant with good bioactivity and cytocompatibility...
  27. ncbi request reprint Changes in dendritic cell function in the immune response to sepsis. Cell- & tissue-based therapy
    Xin Huang
    Brown University School of Medicine, Division of Surgical Research, Department of Surgery, Aldrich 227, Rhode Island Hospital, Providence, RI 02903, USA
    Expert Opin Biol Ther 7:929-38. 2007
    ..Undoubtedly, a better understanding of the dendritic cell's response during sepsis will be critical to developing novel strategies for fighting this deadly disease...
  28. ncbi request reprint Expression and prognostic significance of a panel of tissue hypoxia markers in head-and-neck squamous cell carcinomas
    Quynh Thu Le
    Department of Radiation Oncology, Stanford University, Stanford, CA 94305 5847, USA
    Int J Radiat Oncol Biol Phys 69:167-75. 2007
    ..To investigate the expression pattern of hypoxia-induced proteins identified as being involved in malignant progression of head-and-neck squamous cell carcinoma (HNSCC) and to determine their relationship to tumor pO(2) and prognosis...
  29. ncbi request reprint Regulatory T cell populations in sepsis and trauma
    Fabienne Venet
    Division of Surgical Research, Rhode Island Hospital Brown University, 593 Eddy Street, Providence, RI 02903, USA
    J Leukoc Biol 83:523-35. 2008
    ....
  30. doi request reprint Functional mimicry of the active site of glutathione peroxidase by glutathione imprinted selenium-containing protein
    Lei Liu
    State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130023, People s Republic of China
    Biomacromolecules 9:363-8. 2008
    ..Detailed steady-state kinetic studies demonstrated that the novel selenoenzyme followed a ping-pong mechanism similar to the naturally occurring GPx...
  31. doi request reprint Hypoxia-driven proliferation of embryonic neural stem/progenitor cells--role of hypoxia-inducible transcription factor-1alpha
    Tong Zhao
    Department of Brain Protection and Plasticity, Institute of Basic Medical Sciences, Beijing, China
    FEBS J 275:1824-34. 2008
    ..In contrast, knockdown of HIF-1alpha inhibited NPC proliferation induced by 10% O(2). These results demonstrated that moderate hypoxia was more beneficial to NPC proliferation and that HIF-1alpha was critical in this process...
  32. doi request reprint Construction of the active site of glutathione peroxidase on polymer-based nanoparticles
    Xin Huang
    State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, People s Republic of China
    Biomacromolecules 9:1467-73. 2008
    ..The results clearly show that polymeric nanoparticle can be developed as an excellent model for combining most of catalytic factors of enzyme into one scaffold...
  33. doi request reprint Structural characterization and identification of dibenzocyclooctadiene lignans in Fructus Schisandrae using electrospray ionization ion trap multiple-stage tandem mass spectrometry and electrospray ionization Fourier transform ion cyclotron resonance mul
    Xin Huang
    Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China
    Anal Chim Acta 615:124-35. 2008
    ..And these should be useful for the identification of similar compounds and differentiation of similar species from Chinese herbs...
  34. doi request reprint Activity of sunitinib in patients with advanced neuroendocrine tumors
    Matthew H Kulke
    Dana Farber Cancer Institute, 44 Binney St, Boston, MA 02115, USA
    J Clin Oncol 26:3403-10. 2008
    ..We evaluated the efficacy of sunitinib in a two-cohort, phase II study of advanced carcinoid and pancreatic neuroendocrine tumor patients...
  35. ncbi request reprint Two novel isoforms of Adam23 expressed in the developmental process of mouse and human brains
    Y P Sun
    State Key Laboratory of Genetics Engineering, Institute of Genetics, School of Life Science, Fudan University, YiFu Building of Science and Technology, Shanghai 200433, PR China
    Gene 325:171-8. 2004
    ..This temporally changing expression pattern of Adam23 gamma suggests that ADAM23 gamma likely plays an important role in brain development...
  36. doi request reprint Sleeping Beauty transposon-mediated engineering of human primary T cells for therapy of CD19+ lymphoid malignancies
    Xin Huang
    Division of Blood and Marrow Transplantation, Department of Pediatrics, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    Mol Ther 16:580-9. 2008
    ..Taken together, these data indicate that T cells from PB and UCB can be stably modified using a non-viral DNA transfer system, and that such modified T cells may be useful in the treatment of refractory leukemia and lymphoma...
  37. ncbi request reprint Identification and characterization of DEDDL, a human-specific isoform of DEDD
    Xin Huang
    Institute of Immunology, Second Military Medical University, Shanghai, 200433, PR China
    Gene Expr 13:141-53. 2006
    ..Finally, why DEDD and DEDDL are unstable in the overexpression and other studies may be explained by the finding that they are potential substrates of active caspases...
  38. ncbi request reprint Serum proteomics study of the squamous cell carcinoma antigen 1 in tongue cancer
    Xin Huang
    State Key Laboratory of Biotherapy, Sichuan University, Guoxuexiang 37, Chendu City 610041, PR China
    Oral Oncol 42:26-31. 2006
    ..These results indicate that squamous cell carcinoma antigen 1 may be of great potential as the biomarker of tongue cancer and as the potential therapeutic target for gene therapy...
  39. ncbi request reprint Bacterial redox protein azurin induce apoptosis in human osteosarcoma U2OS cells
    Di sheng Yang
    Department of Orthopaedics, Institute of Orthopaedic Research, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hang Zhou 310009, PR China
    Pharmacol Res 52:413-21. 2005
    ....
  40. ncbi request reprint Uncommon mutation, but common amplifications, of the PIK3CA gene in thyroid tumors
    Guojun Wu
    Department of Otolaryngology Head and Neck Surgery, The Johns Hopkins University School of Medicine, 1830 East Monument Street, Suite 333, Baltimore, Maryland 21287, USA
    J Clin Endocrinol Metab 90:4688-93. 2005
    ..As in many other human cancers, overactivation of the phosphotidylinositol 3-kinase (PI3K)/Akt signaling pathway occurs frequently in thyroid cancer, but the mechanism is not completely clear...
  41. ncbi request reprint Supramolecular chirality of the hydrogen-bonded complex Langmuir-Blodgett film of achiral barbituric acid and melamine
    Xin Huang
    CAS Key Laboratory of Colloid and Interface Science, Institute of Chemistry, CAS, 100080, Beijing, People s Republic of China
    J Colloid Interface Sci 285:680-5. 2005
    ..Increasing the subphase temperature will lead to the decrease of CD intensity and the change of the morphologies. We suggested that the strength of the hydrogen bonding resulted in the difference...
  42. ncbi request reprint [Selective inducement effect of bacterial redox protein azurin on apoptosis of human osteosarcoma cell line U2OS]
    Zhao ming Ye
    Department of Orthopedics, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310009, P R China
    Ai Zheng 24:298-304. 2005
    ..The mechanism of its action is unknown. This study was designed to explore the effects of azurin on apoptosis of osteosarcoma cell line U2OS, and its molecular mechanism...
  43. ncbi request reprint Diverse CD8+ T-cell responses to renal cell carcinoma antigens in patients treated with an autologous granulocyte-macrophage colony-stimulating factor gene-transduced renal tumor cell vaccine
    Xianzheng Zhou
    Division of Immunology and Hematopoiesis, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, Maryland, USA
    Cancer Res 65:1079-88. 2005
    ....
  44. ncbi request reprint Splenic stroma drives mature dendritic cells to differentiate into regulatory dendritic cells
    Minghui Zhang
    The Institute of Immunology, Second Military Medical University, Shanghai, China
    Nat Immunol 5:1124-33. 2004
    ..Thus, our findings identify an important mechanism by which the microenvironment regulates immune responses...
  45. ncbi request reprint 5-HT2A/2C receptor and 5-HT transporter densities in mice prone or resistant to chronic high-fat diet-induced obesity: a quantitative autoradiography study
    Xu Feng Huang
    Molecular Neurobiology Laboratory, Smart Food Center, Department of Biomedical Science, University of Wollongong, Northfield Avenue, Wollongong, NSW 2522, Australia
    Brain Res 1018:227-35. 2004
    ..It provides neural anatomical bases by which genetic variability in 5-HT2A/2C receptors and 5-HT transporter may influence satiety and sensory aspects of energy balance...
  46. ncbi request reprint Lentivirus-mediated gene transfer and expression in established human tumor antigen-specific cytotoxic T cells and primary unstimulated T cells
    Xianzheng Zhou
    Division of Immunology and Hematppoiesis, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA
    Hum Gene Ther 14:1089-105. 2003
    ..These results will have significant implications for the study of T-cell biology and for the improvement of clinical gene therapies of acquired immune deficiency syndrome (AIDS) and cancer...
  47. ncbi request reprint [Changes and significance of tumor necrosis factor-alpha and interleukin-6 level in plasma and bile during the formation of acute intrahepatic cholestasis in New Zealand white rabbits]
    Zhi Hua Huang
    Department of Pediatrics, Tongji Hospital Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
    Zhonghua Gan Zang Bing Za Zhi 11:313. 2003
  48. ncbi request reprint Site-directed mutagenesis and functional analysis of an active site tryptophan of insect chitinase
    Hong Zhang
    Department of Biochemistry, Kansas State University, Manhattan, Kansas 66506, USA
    Insect Biochem Mol Biol 32:1477-88. 2002
    ..The variations in kcat's among the mutated enzymes and the IC50 for the transition state inhibitor analog, allosamidin, indicate that W145 also influences formation of the transition state during catalysis...
  49. ncbi request reprint Adenovirus-mediated tissue-specific cytosine deaminase gene therapy for human hepatocellular carcinoma with different AFP expression levels
    Xin Huang
    Department of Immunology, Second Military Medical University, 800 Xiang Yin Road, Shanghai 200433, P R China
    J Exp Ther Oncol 2:100-6. 2002
    ..Therefore, adenovirus-mediated tumor-specific gene transfer may be a potential strategy for local control of tumor growth...
  50. ncbi request reprint Contribution of anti-inflammatory/immune suppressive processes to the pathology of sepsis
    Mario Perl
    Division of Surgical Research, Department of Surgery, RI Hospital, Brown University School of Medicine, Providence, RI 02903, USA
    Front Biosci 11:272-99. 2006
    ....
  51. ncbi request reprint Pharmacologic and toxicologic evaluation of C. novyi-NT spores
    Luis A Diaz
    Howard Hughes Medical Institute, The Johns Hopkins School of Medicine, Baltimore, MD 21231, USA
    Toxicol Sci 88:562-75. 2005
    ..However, there was no laboratory or histopathologic evidence of sepsis, and the toxicity could be effectively controlled by simple hydration...
  52. pmc Stable gene transfer and expression in human primary T cells by the Sleeping Beauty transposon system
    Xin Huang
    Division of Blood and Marrow Transplantation, Department of Pediatrics, University of Minnesota Cancer Center, Minneapolis, MN 55455, USA
    Blood 107:483-91. 2006
    ..This study is the first report demonstrating that the SB transposon system can mediate stable gene transfer in human primary PBLs, which may be advantageous for T-cell-based gene therapies...
  53. ncbi request reprint Improved performance in protein secondary structure prediction by combining multiple predictions
    De Shuang Huang
    Intelligent Computing Lab, Hefei Institute of Intelligent Machines, CAS, P O Box 1130, Hefei, Anhui, China
    Protein Pept Lett 13:985-91. 2006
    ..The experimental results show that our proposed framework can indeed improve the prediction accuracy...
  54. ncbi request reprint A bacterial protein enhances the release and efficacy of liposomal cancer drugs
    Ian Cheong
    Howard Hughes Medical Institute and the Ludwig Center for Cancer Genetics and Therapeutics, Johns Hopkins Kimmel Comprehensive Cancer Center, Baltimore, MD 21231, USA
    Science 314:1308-11. 2006
    ..This protein could potentially be incorporated into diverse experimental approaches for the specific delivery of chemotherapeutic agents to tumors...
  55. ncbi request reprint A liquid chromatography-mass spectrometry method for the quantitation of aristololactam-I in rat plasma
    Zhen Ling
    New Drug Screen Centre, China Pharmaceutical University, Nanjing, 210038, People s Republic of China
    Biomed Chromatogr 21:10-4. 2007
    ..Absolute recovery of aristololactam-I was 90.4-97.3%. The LC-MS method for the determination of aristololactam-I is sensitive, specific and can be used to investigate the toxicokinetics of aristololactam-I...
  56. ncbi request reprint The genome and transcriptomes of the anti-tumor agent Clostridium novyi-NT
    Chetan Bettegowda
    The Howard Hughes Medical Institute, The Ludwig Center for Cancer Genetics and Therapeutics, The Sidney Kimmel Comprehensive Cancer Center, Department of Pharmacology and Molecular Sciences, Johns Hopkins Medical Institutions, Baltimore, Maryland 21231, USA
    Nat Biotechnol 24:1573-80. 2006
    ..Through this analysis, we found that C. novyi-NT spores contained mRNA and that the spore transcripts were distinct from those in vegetative forms of the bacterium...
  57. pmc PigGIS: Pig Genomic Informatics System
    Jue Ruan
    Beijing Institute of Genomics of the Chinese Academy of Sciences, Beijing Genomics Institute, Beijing 101300, China
    Nucleic Acids Res 35:D654-7. 2007
    ..We have also recovered 18 700 SNPs and 20 800 unique 60mer oligonucleotide probes for future pig genome analyses. PigGIS can be freely accessed via the web at http://www.piggis.org/ and http://pig.genomics.org.cn/...
  58. ncbi request reprint Efficacy and safety of sunitinib in patients with advanced gastrointestinal stromal tumour after failure of imatinib: a randomised controlled trial
    George D Demetri
    Ludwig Center at Dana Farber Cancer Institute and Harvard Medical School, Boston, MA 02115, USA
    Lancet 368:1329-38. 2006
    ....
  59. ncbi request reprint Photoelectron spectroscopy of doubly and singly charged group VIB dimetalate anions: M2O72-, MM'O72-, and M2O7- (M, M' = Cr, Mo, W)
    Hua Jin Zhai
    Department of Physics, Washington State University, 2710 University Drive, Richland, WA 99354, USA
    J Phys Chem A 109:10512-20. 2005
    ..The clusters identified might provide insights into the possible structures of reactive species present in early transition-metal oxide catalysts that are relevant to their reactivity and catalytic function...
  60. ncbi request reprint Proper levels of c-Myb are discretely defined at distinct steps of hematopoietic cell development
    Hiroshi Sakamoto
    Department of Cell Differentiation, Institute of Molecular Embryology and Genetics, Kumamoto University, 2 2 1 Honjo, Kumamoto 860 0811, Japan
    Blood 108:896-903. 2006
    ....
  61. ncbi request reprint Cyclodextrin-derived mimic of glutathione peroxidase exhibiting enzymatic specificity and high catalytic efficiency
    Ze Yuan Dong
    Key Laboratory for Supramolecular Structure and Materials of Ministry of Education, Jilin University, Changchun 130012, China
    Chemistry 12:3575-9. 2006
    ..Importantly, the proposed mode of action of 2-TeCD imitates the role played by several possible noncovalent interactions between enzymes and substrates in influencing catalysis and binding...
  62. ncbi request reprint Determination of glycyrrhetic acid in human plasma by LC-ESI-MS
    Li Ding
    Department of Pharmaceutical Analysis, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, China
    J Pharm Biomed Anal 40:758-62. 2006
    ..51 +/- 3.82%. The method has been successfully applied to study the pharmacokinetics of glycyrrhetic acid in healthy male Chinese volunteers. The assay was proved to be sensitive, accurate and convenient...
  63. ncbi request reprint Prediction of protein secondary structure using improved two-level neural network architecture
    Xin Huang
    Hefei Institute of Intelligent Machines, Chinese Academy of Sciences, P O Box 1130, Hefei, Anhui 230031, China
    Protein Pept Lett 12:805-11. 2005
    ..The experimental results show that our proposed method can efficiently improve the prediction accuracy...
  64. pmc A soluble tissue factor-annexin V chimeric protein has both procoagulant and anticoagulant properties
    Xin Huang
    Department of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    Blood 107:980-6. 2006
    ....
  65. ncbi request reprint Mutant tyrosine kinases with unnatural nucleotide specificity retain the structure and phospho-acceptor specificity of the wild-type enzyme
    Laurie A Witucki
    Department of Cellular and Molecular Pharmacology, Box 0450, University of California, San Francisco 94143 0450, USA
    Chem Biol 9:25-33. 2002
    ..The specificity profiles for the mutant kinases were found to be identical to those of their wild-type counterparts...