Stephen Wagner

Summary

Affiliation: American Red Cross
Country: USA

Publications

  1. ncbi Factors affecting virus photoinactivation by a series of phenothiazine dyes
    S J Wagner
    American Red Cross Biomedical Services, Jerome H Holland Laboratory for the Biomedical Sciences, Rockville, MD 20855, USA
    Photochem Photobiol 67:343-9. 1998
  2. ncbi Transfusion-transmitted bacterial infection: risks, sources and interventions
    Stephen J Wagner
    Vox Sang 88:60. 2005
  3. ncbi Prestorage leucoreduction improves several in vitro red cell storage parameters following gamma irradiation
    S J Wagner
    American Red Cross, Holland Laboratory for the Biomedical Sciences, Blood Components Department, Rockville, MD 20855, USA
    Transfus Med 16:261-5. 2006
  4. ncbi Comparison of the in vitro storage properties of Amicus apheresis platelets collected using single- and double-needle procedures from the same donors
    Stephen J Wagner
    Blood Components Department, American Red Cross Holland Laboratory, Rockville, Maryland 20855, USA
    Transfusion 52:524-8. 2012
  5. ncbi Photoinactivation of Leishmania donovani infantum in red cell suspensions by a flexible thiopyrylium sensitizer
    S J Wagner
    American Red Cross Holland Laboratory for the Biomedical Sciences, Rockville, MD 20855, USA
    Vox Sang 91:178-80. 2006
  6. ncbi Use of a red cell band 3-ligand/antioxidant to improve red cell storage properties following virucidal phototeatment with chalcogenoxanthylium photosensitizers
    Stephen J Wagner
    Blood Components Department, American Red Cross Holland Laboratory for the Biomedical Sciences, Rockville, MD, USA
    Photochem Photobiol 82:1595-600. 2006
  7. ncbi A model to predict the improvement of automated blood culture bacterial detection by doubling platelet sample volume
    Stephen J Wagner
    Blood Components Department, American Red Cross Holland Laboratory for the Biomedical Sciences, Rockville, Maryland 20855, USA
    Transfusion 47:430-3. 2007
  8. ncbi Photoinactivation of Trypanosoma cruzi in red cell suspensions with thiopyrylium
    Stephen J Wagner
    American Red Cross Holland Laboratory, Rockville, MD 20855, USA
    Transfus Apher Sci 37:23-5. 2007
  9. ncbi The influence of simulated shipping conditions (24- or 30-hr interruption of agitation) on the in vitro properties of apheresis platelets during 7-day storage
    Stephen J Wagner
    Blood Components Department, American Red Cross, Holland Laboratory, Rockville, MD 20855, USA
    Transfusion 48:1072-80. 2008
  10. ncbi Developing pathogen reduction technologies for RBC suspensions
    S J Wagner
    Blood Components Department, American Red Cross Holland Laboratory, Rockville, MD 20855, USA
    Vox Sang 100:112-21. 2011

Research Grants

  1. VIRUS PHOTOINACTIVATION--DIMETHYLMETHYLENE BLUE IN RBC
    Stephen Wagner; Fiscal Year: 2004

Detail Information

Publications42

  1. ncbi Factors affecting virus photoinactivation by a series of phenothiazine dyes
    S J Wagner
    American Red Cross Biomedical Services, Jerome H Holland Laboratory for the Biomedical Sciences, Rockville, MD 20855, USA
    Photochem Photobiol 67:343-9. 1998
    ....
  2. ncbi Transfusion-transmitted bacterial infection: risks, sources and interventions
    Stephen J Wagner
    Vox Sang 88:60. 2005
  3. ncbi Prestorage leucoreduction improves several in vitro red cell storage parameters following gamma irradiation
    S J Wagner
    American Red Cross, Holland Laboratory for the Biomedical Sciences, Blood Components Department, Rockville, MD 20855, USA
    Transfus Med 16:261-5. 2006
    ..Based on the in vivo survival studies of Davey et al. and the results of this study, there is justification to consider performing additional survival studies to support extended storage of LR gamma-irradiated RBC...
  4. ncbi Comparison of the in vitro storage properties of Amicus apheresis platelets collected using single- and double-needle procedures from the same donors
    Stephen J Wagner
    Blood Components Department, American Red Cross Holland Laboratory, Rockville, Maryland 20855, USA
    Transfusion 52:524-8. 2012
    ..To investigate whether the method of PLT collection using the same instrument influences PLT quality, the in vitro storage properties of Amicus PLTs were evaluated in the same donors collected by SN and DN procedures...
  5. ncbi Photoinactivation of Leishmania donovani infantum in red cell suspensions by a flexible thiopyrylium sensitizer
    S J Wagner
    American Red Cross Holland Laboratory for the Biomedical Sciences, Rockville, MD 20855, USA
    Vox Sang 91:178-80. 2006
    ..There is no licensed Leishmania screening test currently available...
  6. ncbi Use of a red cell band 3-ligand/antioxidant to improve red cell storage properties following virucidal phototeatment with chalcogenoxanthylium photosensitizers
    Stephen J Wagner
    Blood Components Department, American Red Cross Holland Laboratory for the Biomedical Sciences, Rockville, MD, USA
    Photochem Photobiol 82:1595-600. 2006
    ....
  7. ncbi A model to predict the improvement of automated blood culture bacterial detection by doubling platelet sample volume
    Stephen J Wagner
    Blood Components Department, American Red Cross Holland Laboratory for the Biomedical Sciences, Rockville, Maryland 20855, USA
    Transfusion 47:430-3. 2007
    ..This study analyses the projected benefit in detecting slow growing organisms by increasing sample volume from 4 to 8 mL...
  8. ncbi Photoinactivation of Trypanosoma cruzi in red cell suspensions with thiopyrylium
    Stephen J Wagner
    American Red Cross Holland Laboratory, Rockville, MD 20855, USA
    Transfus Apher Sci 37:23-5. 2007
    ....
  9. ncbi The influence of simulated shipping conditions (24- or 30-hr interruption of agitation) on the in vitro properties of apheresis platelets during 7-day storage
    Stephen J Wagner
    Blood Components Department, American Red Cross, Holland Laboratory, Rockville, MD 20855, USA
    Transfusion 48:1072-80. 2008
    ..Studies have demonstrated maintenance of PLT quality of whole blood-derived PLT concentrates during a 24-hour interruption of agitation, but data are not available for apheresis PLTs in 100 percent plasma...
  10. ncbi Developing pathogen reduction technologies for RBC suspensions
    S J Wagner
    Blood Components Department, American Red Cross Holland Laboratory, Rockville, MD 20855, USA
    Vox Sang 100:112-21. 2011
    ..Clinical trials will ultimately be necessary to further develop either flexible photosensitizers or nucleic acid alkylating methods with quenchers for use in Transfusion Medicine...
  11. ncbi Maintenance of platelet in vitro properties during 7-day storage in M-sol with a 30-hour interruption of agitation
    Stephen J Wagner
    Blood Components Department, American Red Cross, Holland Laboratory, Rockville, Maryland, USA
    Transfusion 48:2501-607. 2008
    ..A newly described bicarbonate-containing AS, M-sol, was compared to plasma for preservation of whole blood-derived PLT concentrates in which a 30-hour interruption of agitation was included...
  12. ncbi Comparison of the in vitro properties of apheresis platelets during 7-day storage after interrupting agitation for one or three periods
    Stephen J Wagner
    Blood Components Department, American Red Cross, Holland Laboratory, Rockville, Maryland, USA
    Transfusion 48:2492-500. 2008
    ..We have previously conducted studies investigating maintenance of apheresis PLT in vitro quality measures during a single 24- or 30-hour interruption of agitation, but data are not available for multiple periods without agitation...
  13. ncbi Evaluation of in vitro storage properties of prestorage pooled whole blood-derived platelets suspended in 100 percent plasma and treated with amotosalen and long-wavelength ultraviolet light
    Stephen J Wagner
    American Red Cross Biomedical Services, 15601 Crabbs Branch Way, Rockville, MD 20855, USA
    Transfusion 49:704-10. 2009
    ..This study investigated the in vitro PLT properties of amotosalen-PCT of leukoreduced pools of PLTs prepared by the PRP method and suspended in 100 percent plasma...
  14. ncbi Inactivation of Leishmania donovani infantum and Trypanosoma cruzi in red cell suspensions with thiazole orange
    Stephen J Wagner
    American Red Cross, Holland Laboratory for the Biomedical Sciences, Blood Components Department, Rockville, Maryland 20855, USA
    Transfusion 48:1363-7. 2008
    ..The aim of this study is to further evaluate the ability of TO to inactivate pathogens by measuring its activity against the protozoa Leishmania donovani infantum and Trypanosoma cruzi...
  15. ncbi The maintenance of platelet properties during 20-24 degrees C storage after periods of shipment or interrupted agitation
    Stephen J Wagner
    American Red Cross Holland Laboratory, 15601 Crabbs Branch Way, Rockville, MD 20855, USA
    Transfus Apher Sci 42:45-51. 2010
    ..This review will examine the outcomes of these studies and consider new developments in platelet storage which might improve platelet quality after interruptions of agitation which occur during platelet shipment...
  16. ncbi Calcium is a key constituent for maintaining the in vitro properties of platelets suspended in the bicarbonate-containing additive solution M-sol with low plasma levels
    Stephen J Wagner
    American Red Cross, Holland Laboratory, Rockville, Maryland 20855, USA
    Transfusion 50:1028-35. 2010
    ..PLTs suspended in M-sol, a bicarbonate-based experimental platelet additive solution (PAS), maintain in vitro PLT properties during storage with low levels of plasma (< or =5%)...
  17. ncbi Influence of apheresis container size on the maintenance of platelet in vitro storage properties after a 30-h interruption of agitation
    Stephen J Wagner
    Blood Components Department, American Red Cross, Holland Laboratory, Rockville, MD 20855, USA
    Transfus Apher Sci 43:9-15. 2010
    ..17+/-06 and 0.37+/-0.09 pH units greater, n=4, respectively). Other measures showed either small differences or comparability of platelet in vitro parameters with storage in the two containers after an interruption of agitation...
  18. ncbi Toward closed-system culture of blood origin endothelial cells
    Stephen J Wagner
    Blood Components Department, American Red Cross Holland Laboratory, Rockville, Maryland 20855, USA
    Transfusion 45:1201-7. 2005
    ....
  19. ncbi Use of a flexible thiopyrylium photosensitizer and competitive inhibitor for pathogen reduction of viruses and bacteria with retention of red cell storage properties
    Stephen J Wagner
    American Red Cross Biomedical Services, Holland Laboratory, Blood and Cell Therapy Development, Rockville, Maryland, USA
    Transfusion 45:752-60. 2005
    ....
  20. ncbi Monocyte enrichment of mononuclear apheresis preparations with a multistep back-flush procedure on a cord blood filter
    Stephen J Wagner
    American Red Cross Biomedical Services, Holland Laboratory, Blood and Cell Therapy Development, Rockville, Maryland 20855, USA
    Transfusion 45:433-9. 2005
    ....
  21. ncbi Quinacrine enhances vesicular stomatitis virus inactivation and diminishes hemolysis of dimethylmethylene blue-phototreated red cells
    Stephen J Wagner
    American Red Cross Biomedical Services, Jerome H Holland Laboratory for the Biomedical Sciences, Blood and Cell Therapy Development, Rockville, MD 20855, USA
    Photochem Photobiol 76:514-7. 2002
    ..These results collectively suggest that despite its recognized affinity for viral nucleic acid, DMMB also binds to RBC membranes and that the bound dye is, in part, responsible for photoinduced hemolysis...
  22. ncbi Use of a flow-cell system to investigate virucidal dimethylmethylene blue phototreatment in two RBC additive solutions
    Stephen Wagner
    Jerome H Holland Laboratory for the Biomedical Sciences, American Red Cross Biomedical Services, Rockville, Maryland 20855, USA
    Transfusion 42:1200-5. 2002
    ..A flow-cell system was developed to rapidly treat larger volumes of oxygenated 45 percent Hct RBCs with high-intensity red light...
  23. ncbi Transfusion-transmitted bacterial infection: risks, sources and interventions
    S J Wagner
    Biomedical Research and Development, American Red Cross, Rockville, MD 20855, USA
    Vox Sang 86:157-63. 2004
    ....
  24. ncbi Duck hepatitis B photoinactivation bydimethylmethylene blue in RBC suspensions
    S J Wagner
    American Red Cross Holland Laboratory for the Biomedical Sciences, Rockville, Maryland, USA
    Transfusion 41:1154-8. 2001
    ....
  25. ncbi Evaluation of the sensitivity of red blood cell markers to detect photodynamic membrane damage
    Stephen J Wagner
    Blood and Cell Therapy Development, American Red Cross, Biomedical Research and Development, Rockville, Maryland 20855, USA
    Transfusion 44:716-21. 2004
    ..This study compares the relative sensitivity of three assays for detecting RBC membrane damage...
  26. ncbi Diversion of initial blood flow to prevent whole-blood contamination by skin surface bacteria: an in vitro model
    S J Wagner
    Product Development Department, Holland Laboratory for the Biomedical Sciences, American Red Cross Biomedical Services, Rockville, MD 20855, USA
    Transfusion 40:335-8. 2000
    ..A model system was used to investigate if a skin organism's bioburden in blood components could be reduced by diverting the first few mL of whole blood away from the primary container...
  27. ncbi Investigation of photosensitizing dyes for pathogen reduction in red cell suspensions
    S J Wagner
    American Red Cross, Biomedical Research and Development, Blood and Cell Therapy Development, Rockville, MD 20855, USA
    Biotech Histochem 78:171-7. 2003
    ....
  28. ncbi Sterile isolation and cryopreservation of human adherent monocytes/macrophages from mononuclear cell apheresis preparations
    Stephen J Wagner
    American Red Cross, Biomedical Services, Jerome H Holland Laboratory for the Biomedical Sciences, Blood and Cell Therapy Development, Rockville, Maryland 20855, USA
    Wound Repair Regen 11:145-9. 2003
    ..g., whether administration of exogenously supplied cells improves the healing of chronic wounds or promotes the regeneration of transected nerves...
  29. ncbi Red cell alterations associated with virucidal methylene blue phototreatment
    S J Wagner
    Product Development Laboratory, American Red Cross Blood Services, Rockville, Maryland
    Transfusion 33:30-6. 1993
    ..Taken together, these data suggest that the red cell surface is altered by virucidal MB phototreatment of vesicular stomatitis virus...
  30. ncbi Bacterial screening of apheresis platelets and the residual risk of septic transfusion reactions: the American Red Cross experience (2004-2006)
    Anne F Eder
    Biomedical Services, Medical Office, National Headquarters, American Red Cross, Washington, DC 20006, USA
    Transfusion 47:1134-42. 2007
    ..The American Red Cross initiated systemwide bacterial testing of all apheresis platelet (PLT) collections in March 2004, yet continues to receive reports of septic reactions after transfusion of screened components...
  31. ncbi Research opportunities for pathogen reduction/inactivation of blood components: summary of an NHLBI workshop
    Harvey G Klein
    National Institutes of Health, and Division of Blood Diseases and Resources, National Heart, Lung, and Blood Institute, NIH, Bethesda, MD, USA
    Transfusion 49:1262-8. 2009
    ....
  32. ncbi Inactivation of WBCs in RBC suspensions by photoactive phenothiazine dyes: comparison of dimethylmethylene blue and MB
    A A Skripchenko
    Product Development Department, Jerome Holland Laboratory, American Red Cross, Rockville, Maryland 20855 2734, USA
    Transfusion 40:968-75. 2000
    ..The present study compares WBC photoinactivation in buffy coat after DMMB or MB phototreatment under virucidal conditions...
  33. ncbi In vivo recovery and survival of red cells after photodynamic treatment with thiopyrylium and red light using a canine model
    A Skripchenko
    Holland Laboratory for the Biomedical Sciences, American Red Cross, 15601 Crabbs Branch Way, Rockville, MD 20855, USA
    Vox Sang 92:157-9. 2007
    ..255 +/- 63 h, respectively). Results suggest that thiopyrylium phototreatment does not negatively affect canine RBC in vivo recovery...
  34. ncbi Determination of residual 4'-aminomethyl-4,5',8-trimethylpsoralen and mutagenicity testing following psoralen plus UVA treatment of platelet suspensions
    S J Wagner
    American Red Cross Blood Services, Jerome H Holland Laboratory for the Biomedical Sciences, Rockville, MD 29855
    Photochem Photobiol 57:819-24. 1993
    ..6 J/cm2 UVA irradiation, and 8 to 10% of total AMT was associated with saline-washed irradiated platelets and is presumably tightly bound.(ABSTRACT TRUNCATED AT 250 WORDS)..
  35. ncbi Synthesis of analogues of a flexible thiopyrylium photosensitizer for purging blood-borne pathogens and binding mode and affinity studies of their complexes with DNA
    Ruel E McKnight
    Department of Chemistry, State University of New York at Geneseo, 1 College Circle, Geneseo, NY 14454, USA
    Bioorg Med Chem 15:4406-18. 2007
    ..The dyes were found to show mixed binding modes...
  36. ncbi Virus inactivation in blood components by photoactive phenothiazine dyes
    Stephen J Wagner
    American Red Cross Blood Services, Holland Laboratory for the Biomedical Sciences, Rockville, MD, USA
    Transfus Med Rev 16:61-6. 2002
    ..Future widespread use of phenothiazines for virus photoinactivation in blood components may depend on whether the risk inherent in their use is less than the residual risk from transfusion-transmitted viruses...
  37. ncbi Platelet products prepared by different methods of sedimentation undergo platelet activation differently during storage
    Andrey Skripchenko
    The American Red Cross Biomedical Services, Holland Laboratory, Blood Components Development, Rockville, Maryland 20855, USA
    Transfusion 48:1469-77. 2008
    ....
  38. ncbi "Switched-on" flexible chalcogenopyrylium photosensitizers. Changes in photophysical properties upon binding to DNA
    Tymish Y Ohulchanskyy
    Institute for Lasers, Photonics, and Biophotonics, Department of Chemistry, University at Buffalo, The State University of New York, Buffalo, New York 14260, USA
    J Phys Chem B 111:9686-92. 2007
    ....
  39. ncbi Current clinical and preclinical photosensitizers for use in photodynamic therapy
    Michael R Detty
    Department of Chemistry, University at Buffalo, The State University of New York, Buffalo, New York 14260-3000, USA
    J Med Chem 47:3897-915. 2004
  40. ncbi Thiazole orange, a DNA-binding photosensitizer with flexible structure, can inactivate pathogens in red blood cell suspensions while maintaining red cell storage properties
    Andrey Skripchenko
    American Red Cross Biomedical Services, Holland Laboratory, Blood Components Development, Rockville, Maryland 20855, USA
    Transfusion 46:213-9. 2006
    ....
  41. ncbi Chalcogenoxanthylium photosensitizers for the photodynamic purging of blood-borne viral and bacterial pathogens
    Stephen J Wagner
    American Red Cross, Holland Laboratory for the Biomedical Sciences, Rockville, MD 20855, USA
    Bioorg Med Chem 13:5927-35. 2005
    ..Photocleavage experiments with plasmid DNA and the chalcogenoxanthylium dyes suggested the genomic material contained in the virus and in the bacteria as one possible target for the photodynamic action of some of these dyes...
  42. ncbi The residual risk of sepsis: modeling the effect of concentration on bacterial detection in two-bottle culture systems and an estimation of false-negative culture rates
    Richard J Benjamin
    Biomedical Services, Medical Office, National Headquarters, American Red Cross, 2025 E Street NW, Washington, DC 20006, USA
    Transfusion 47:1381-9. 2007
    ..The mechanism of test failure was modeled and false-negative rates were estimated utilizing published data...

Research Grants5

  1. VIRUS PHOTOINACTIVATION--DIMETHYLMETHYLENE BLUE IN RBC
    Stephen Wagner; Fiscal Year: 2004
    ..abstract_text> ..