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Genomes and Genes | K D BuntingSummaryAffiliation: American Red Cross Country: USA Publications
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Publications
Reduced lymphomyeloid repopulating activity from adult bone marrow and fetal liver of mice lacking expression of STAT5Kevin D Bunting
Hematopoiesis Department, American Red Cross Holland Laboratory, Rockville, MD, USA
Blood 99:479-87. 2002..These results demonstrate that a significant proportion of the growth factor signals required for multilineage reconstitution potential of HSCs is STAT5 dependent...- Increased numbers of committed myeloid progenitors but not primitive hematopoietic stem/progenitors in mice lacking STAT6 expressionKevin D Bunting
American Red Cross, Jerome H Holland Laboratory for the Biomedical Sciences, 15601 Crabbs Branch Way, Rockville, MD 20855, USA
J Leukoc Biol 76:484-90. 2004..Therefore, the requirement for STAT6 activation during hematopoiesis is limited, and primitive hematopoietic cell types are insulated against possible effects of cytokine stimulation by Th1 cells... - Self-selection by genetically modified committed lymphocyte precursors reverses the phenotype of JAK3-deficient mice without myeloablationK D Bunting
Division of Experimental Hematology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
Hum Gene Ther 11:2353-64. 2000..This work shows that the large selective advantage for JAK3-corrected lymphoid cells may be sufficient to overcome the need for myeloablative conditioning in JAK3 gene therapy protocols... - ABC transporters as phenotypic markers and functional regulators of stem cellsKevin D Bunting
Hematopoiesis Department, American Red Cross Holland Laboratory, Rockville, Maryland 20855, USA
Stem Cells 20:11-20. 2002..In light of these findings, it will be critical to further characterize this family of proteins in hematopoietic lineage-restricted stem cells and in pluripotent stem cells capable of crossing lineage barriers... - Enforced P-glycoprotein pump function in murine bone marrow cells results in expansion of side population stem cells in vitro and repopulating cells in vivoK D Bunting
Division of Experimental Hematology, Department of Hematology Oncology, and the Department of Biochemistry, St Jude Children s Research Hospital, Memphis, TN 38105, USA
Blood 96:902-9. 2000..These studies further support the concept that ABC transporter function can have important effects on hematopoietic stem cell development... - Stat5a/b contribute to interleukin 7-induced B-cell precursor expansion, but abl- and bcr/abl-induced transformation are independent of stat5V Sexl
Howard Hughes Medical Institute, Departments of Biochemistry, Tumor Cell Biology, Immunology, and Experimental Hematology, St Jude Children s Research Hospital, Memphis, TN 38105, USA
Blood 96:2277-83. 2000..We demonstrate that the absence of Stat5a/b is not essential for the induction of lymphoid or myeloid tumors in vivo or on the ability to transform bone marrow cells in vitro... 
Jak3 selectively regulates Bax and Bcl-2 expression to promote T-cell developmentR Wen
Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
Mol Cell Biol 21:678-89. 2001....- Restoration of lymphocyte function in Janus kinase 3-deficient mice by retroviral-mediated gene transferK D Bunting
Division of Experimental Hematology, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
Nat Med 4:58-64. 1998..These results show that gene replacement is a feasible treatment strategy for this disease and that naturally occurring in vivo selection of corrected cells is an important advantage of this approach... - Stat5 is required for IL-2-induced cell cycle progression of peripheral T cellsR Moriggl
Howard Hughes Medical Institute, and Department of Biochemistry, St Jude Children s Research Hospital, Memphis, Tennessee 38105, USA
Immunity 10:249-59. 1999..These phenotypes are not seen in mice lacking Stat5a or Stat5b alone. The results demonstrate that the Stat5 proteins, redundantly, are essential mediators of IL-2 signaling in T cells... - The ABC transporter Bcrp1/ABCG2 is expressed in a wide variety of stem cells and is a molecular determinant of the side-population phenotypeS Zhou
Division of Experimental Hematology, Department of Hematology Oncology, St Jude Children s Research Hospital, Memphis, Tennessee, USA
Nat Med 7:1028-34. 2001..These results show that expression of the Bcrp1/ABCG2 gene is an important determinant of the SP phenotype, and that it might serve as a marker for stem cells from various sources... - Transduction of murine bone marrow cells with an MDR1 vector enables ex vivo stem cell expansion, but these expanded grafts cause a myeloproliferative syndrome in transplanted miceK D Bunting
Division of Experimental Hematology, The Department of Immunology, and the Department of Biochemistry, St Jude Children s Research Hospital, Memphis, TN, USA
Blood 92:2269-79. 1998..These results show that MDR1-transduced stem cells can be expanded in vitro using hematopoietic cytokines without any drug selection, but enforced stem cell self-renewal divisions can have adverse consequences... - Leukemias following retroviral transfer of multidrug resistance 1 (MDR1) are driven by combinatorial insertional mutagenesisUte Modlich
Department of Hematology, Hemostaseology and Oncology, Institute of Cellular and Molecular Pathology, Hannover Medical School, Germany
Blood 105:4235-46. 2005..On the basis of these findings, we suggest the use of preclinical dose-escalation studies to define a therapeutic index for retroviral transgene delivery... - Effects of a leukemia-associated gain-of-function mutation of SHP-2 phosphatase on interleukin-3 signalingWen Mei Yu
Department of Medicine, Division of Hematology Oncology, Case Comprehensive Cancer Center, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA
J Biol Chem 281:5426-34. 2006.... - Hematopoietic reconstitution with androgenetic and gynogenetic stem cellsSigrid Eckardt
Center for Animal Transgenesis and Germ Cell Research, New Bolton Center, University of Pennsylvania, Kennett Square, Pennsylvania 19348, USA
Genes Dev 21:409-19. 2007.... - Abnormal hematopoiesis in Gab2 mutant miceYi Zhang
Department of Medicine, Division of Hematology, Case Western Reserve University School of Medicine, Cleveland, OH 44106 7284, USA
Blood 110:116-24. 2007..Therefore, we demonstrate that Gab2 adapter function is intrinsically required for hematopoietic cell response to early-acting cytokines, resulting in defective hematopoiesis in Gab2-deficient mice... - Stat5 is essential for early B cell development but not for B cell maturation and functionXuezhi Dai
State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, People s Republic of China
J Immunol 179:1068-79. 2007..Taken together, these data demonstrate that Stat5A/5B directly control IL-7-mediated early B cell development but are not required for B cell maturation and Ig production... - Cited2 is required for normal hematopoiesis in the murine fetal liverYu Chen
Department of Pharmacology, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA
Blood 110:2889-98. 2007..The present study uncovers for the first time a novel role of Cited2 in the maintenance of hematopoietic homeostasis during embryogenesis and thus provides new insights into the molecular regulation of hematopoietic development... - STAT5 requires the N-domain to maintain hematopoietic stem cell repopulating function and appropriate lymphoid-myeloid lineage outputGeqiang Li
Department of Medicine, Division of Hematology Oncology, Case Western Reserve University, School of Medicine, Cleveland, OH 44106, USA
Exp Hematol 35:1684-94. 2007..The goal of these studies was to use transplantation-based assays to analyze the degree of STAT5 deltaN activity in hematopoietic stem cells (HSC) and throughout lymphomyeloid development... 
Retroviral transduction of murine hematopoietic stem cellsPeter Haviernik
Department of Medicine, Division of Hematology Oncology, Center for Stem Cell and Regenerative Medicine, Case Western Reserve University, Cleveland, OH, USA
Methods Mol Biol 430:229-41. 2008..We also describe methods for transplantation and subsequent analysis of transplanted mice as a bona fide assay for the stem cell transduction efficiency...- Stat5 tetramer formation is associated with leukemogenesisRichard Moriggl
Institute of Molecular Pathology, Dr Bohr Gasse 7, A 1030 Vienna, Austria
Cancer Cell 7:87-99. 2005..In addition, Stat5 tetramers were found to accumulate in excess compared to dimers in various human leukemias. These data suggest that Stat5 tetramers are associated with leukemogenesis... - STAT3- and STAT5-dependent pathways competitively regulate the pan-differentiation of CD34pos cells into tumor-competent dendritic cellsPeter A Cohen
Center for Surgery Research, Cleveland Clinic Foundation Lerner Research Institute, OH 44195, USA
Blood 112:1832-43. 2008.... - Cell intrinsic defects in cytokine responsiveness of STAT5-deficient hematopoietic stem cellsHeath L Bradley
Hematopoiesis Department and the Flow Cytometry Facility, American Red Cross Holland Laboratory, Rockville, MD 20855, USA
Blood 100:3983-9. 2002..These studies support a major role for STAT5 activation as a cellular determinant of cytokine-mediated HSC repopulating potential but not self-renewal capacity... - Stat5 expression is critical for mast cell development and survivalChristopher P Shelburne
Department of Biology, Virginia Commonwealth University, Richmond, VA 23284 2012, USA
Blood 102:1290-7. 2003..These results indicate that Stat5A and Stat5B are critical regulators of in vitro and in vivo mast cell development and survival... - The tao of hematopoietic stem cells: toward a unified theory of tissue regenerationKevin D Bunting
Hematopoiesis Department, Cell Therapy Research and Development, American Red Cross Holland Laboratory, 15601 Crabbs Branch Way, Rockville, MD 20855, USA
ScientificWorldJournal 2:983-95. 2002.... - Integrative molecular and developmental biology of adult stem cellsKevin D Bunting
Department of Hematopoiesis, Holland Laboratory, American Red Cross, Rockville, MD 20855, USA
Biol Cell 95:563-78. 2003.... - Hematopoietic-repopulating defects from STAT5-deficient bone marrow are not fully accounted for by loss of thrombopoietin responsivenessHeath L Bradley
Hematopoiesis Department, Jerome H Holland Laboratory for the Biomedical Sciences, American Red Cross, Rockville, MD 20855, USA
Blood 103:2965-72. 2004..These results indicate substantial overlap in c-Mpl-STAT5 signaling defects at the MPP/HSC level but indicate that STAT5 is activated independent of c-Mpl to promote multilineage hematopoietic differentiation... - A negative role of SHP-2 tyrosine phosphatase in growth factor-dependent hematopoietic cell survivalJing Chen
Department of Hematopoiesis, Jerome H Holland Laboratory for the Biomedical Sciences, American Red Cross, 15601 Crabbs Branch Way, Rockville, MD 20855, USA
Oncogene 23:3659-69. 2004..Taken together, these results suggest that SHP-2 tyrosine phosphatase negatively regulates hematopoietic cell survival by dephosphorylation of STAT5... - Cutting edge: IL-5 primes Th2 cytokine-producing capacity in eosinophils through a STAT5-dependent mechanismYuechun Zhu
Department of Cell Biology, Stritch School of Medicine, Loyola University Chicago, Maywood, IL 60153, USA
J Immunol 173:2918-22. 2004..Thus, our study demonstrates that IL-5 functions as a potent factor that drives bone marrow progenitor cells into IL-4-producing eosinophils... - A STAT5 modifier locus on murine chromosome 7 modulates engraftment of hematopoietic stem cells during steady-state hematopoiesisChristine Couldrey
Hematopoiesis Department, Jerome H Holland Laboratory for the Biomedical Sciences, American Red Cross, Rockville, MD, USA
Blood 105:1476-83. 2005..Therefore, we conclude that STAT5 plays a critical role during steady-state HSC engraftment and a chromosome 7 modifier locus regulates this activity... - Stat5 expression is required for IgE-mediated mast cell functionBrian O Barnstein
Department of Biology, Virginia Commonwealth University, Richmond, VA 23284, USA
J Immunol 177:3421-6. 2006..These data have a direct impact on MC-associated inflammatory and autoimmune diseases... - Identification of 6-benzylthioinosine as a myeloid leukemia differentiation-inducing compoundDavid N Wald
Department of Pathology, Case Western Reserve School of Medicine, Cleveland, Ohio 44106, USA
Cancer Res 68:4369-76. 2008..Besides in vitro activity, 6BT also shows the ability to impair HL-60 and MV4-11 tumor growth in nude mice. 6BT is a promising new monocytic differentiation agent with apparent leukemic cell-specific activity... - Hematopoiesis in mice is extremely resilient to wide variation in TIMP/MMP balancePeter Haviernik
Department of Medicine, Division of Hematology Oncology, Case Western Reserve University, Cleveland, OH 44106, USA
Blood Cells Mol Dis 41:179-87. 2008..An important corollary of these studies is that specific modulation using MMP inhibitors for cancer or immunologic therapy is unlikely to have adverse hematopoietic side effects... - Hematopoietic stem cell transplant into non-myeloablated W/Wv mice to detect steady-state engraftment defectsZhengqi Wang
Department of Medicine, Division of Hematology Oncology, Center for Stem Cell and Regenerative Medicine, Case Western Reserve University, Cleveland, OH, USA
Methods Mol Biol 430:171-81. 2008..The ease of this approach is an advantage, and this method may be particularly useful for teasing apart HSC engraftment phenotypes that are especially dependent on functions related to homing, adhesion, or migration into the niche... - The expanding tool kit for hematopoietic stem cell researchWilliam Tse
Department of Medicine, Division of Hematology Oncology, Center for Stem Cell and Regenerative Medicine, Case Western Reserve University, Cleveland, OH, USA
Methods Mol Biol 430:3-18. 2008..This overview chapter summarizes the growing number of tools available for HSC research and specifically ties together the methods in chapters of the second edition of Hematopoietic Stem Cell Protocols... - Safety concerns related to hematopoietic stem cell gene transfer using retroviral vectorsPeter Haviernik
Hematopoiesis Department, Jerome H. Holland Laboratory for the Biomedical Sciences, American Red Cross, Rockville, MD 20855, USA
Curr Gene Ther 4:263-76. 2004..Therefore, vector design and safety testing are at the forefront of the field of gene therapy and this review discusses recent developments... - Bone marrow transplantation combined with gene therapy to induce antigen-specific tolerance and ameliorate EAEBiying Xu
Department of Surgery, University of Maryland, Baltimore, MD 21201, USA
Mol Ther 13:42-8. 2006..Our results suggest that HSC may be useful to achieve long-lasting tolerance to protect mice from EAE and possibly to promote CNS repair in ongoing EAE... - STAT5 signaling in normal and pathologic hematopoiesisKevin D Bunting
Department of Medicine, Division of Hematology Oncology, Case Western Reserve University School of Medicine, Cleveland, OH 44122 7284, USA
Front Biosci 12:2807-20. 2007..leukemic STAT5 activation. This review summarizes the latest understanding of leukemogenesis and pathophysiology associated with constitutive STAT5 activation in hematologic malignancies... - Tissue inhibitor of matrix metalloproteinase-1 overexpression in M1 myeloblasts impairs IL-6-induced differentiationPeter Haviernik
Hematopoiesis Department, American Red Cross, Jerome H Holland Laboratory for the Biomedical Sciences, Rockville, MD 20855, USA
Oncogene 23:9212-9. 2004..Intrinsic TIMP-1 expression in myeloid leukemia cells might thus impact upon survival or differentiation...