D J Prockop

Summary

Affiliation: Allegheny College
Country: USA

Publications

  1. ncbi Marrow stromal cells as stem cells for nonhematopoietic tissues
    D J Prockop
    Center for Gene Therapy, Allegheny University of the Health Sciences, MCP Hahnemann School of Medicine, 245 North 15 Street, Mail Stop 421, Philadelphia, PA 19102, USA
    Science 276:71-4. 1997
  2. ncbi Rat marrow stromal cells are more sensitive to plating density and expand more rapidly from single-cell-derived colonies than human marrow stromal cells
    E H Javazon
    Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, Louisiana 70112, USA
    Stem Cells 19:219-25. 2001
  3. ncbi BMP-6 enhances chondrogenesis in a subpopulation of human marrow stromal cells
    I Sekiya
    Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, Louisiana 70112, USA
    Biochem Biophys Res Commun 284:411-8. 2001
  4. ncbi Plastic adherent stromal cells from the bone marrow of commonly used strains of inbred mice: variations in yield, growth, and differentiation
    D G Phinney
    Center for Gene Therapy, MCP Hahnemann University, Philadelphia, Pennsylvania 19102, USA
    J Cell Biochem 72:570-85. 1999
  5. ncbi Targeted disruption of Col11a2 produces a mild cartilage phenotype in transgenic mice: comparison with the human disorder otospondylomegaepiphyseal dysplasia (OSMED)
    S W Li
    Center for Gene Therapy, MCP Hahnemann University, Philadelphia, Pennsylvania, USA
    Dev Dyn 222:141-52. 2001
  6. ncbi Rat marrow stromal cells rapidly transduced with a self-inactivating retrovirus synthesize L-DOPA in vitro
    E J Schwarz
    Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, LA 70112, USA
    Gene Ther 8:1214-23. 2001
  7. ncbi Correction of a mineralization defect by overexpression of a wild-type cDNA for COL1A1 in marrow stromal cells (MSCs) from a patient with osteogenesis imperfecta: a strategy for rescuing mutations that produce dominant-negative protein defects
    R R Pochampally
    Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, LA 70112, USA
    Gene Ther 12:1119-25. 2005
  8. ncbi Identification of a subpopulation of rapidly self-renewing and multipotential adult stem cells in colonies of human marrow stromal cells
    D C Colter
    Center for Gene Therapy, Tulane University Health Sciences Center, 1430 Tulane Avenue SL-99, New Orleans, LA 70112, USA
    Proc Natl Acad Sci U S A 98:7841-5. 2001
  9. ncbi Isolation and characterization of rapidly self-renewing stem cells from cultures of human marrow stromal cells
    D J Prockop
    Center for Gene Therapy, Tulane University Health Sciences Center, 1430 Tulane Avenue, SL-99, New Orleans, LA 70112, USA
    Cytotherapy 3:393-6. 2001
  10. ncbi Stable transfection of MSCs by electroporation
    A Peister
    Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, LA 70112, USA
    Gene Ther 11:224-8. 2004

Collaborators

Detail Information

Publications19

  1. ncbi Marrow stromal cells as stem cells for nonhematopoietic tissues
    D J Prockop
    Center for Gene Therapy, Allegheny University of the Health Sciences, MCP Hahnemann School of Medicine, 245 North 15 Street, Mail Stop 421, Philadelphia, PA 19102, USA
    Science 276:71-4. 1997
    ..Therefore, marrow stromal cells present an intriguing model for examining the differentiation of stem cells. Also, they have several characteristics that make them potentially useful for cell and gene therapy...
  2. ncbi Rat marrow stromal cells are more sensitive to plating density and expand more rapidly from single-cell-derived colonies than human marrow stromal cells
    E H Javazon
    Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, Louisiana 70112, USA
    Stem Cells 19:219-25. 2001
    ..The rat mMSCs differed from human mMSCs in that they regenerated RS cells in culture. Also, after low-density plating, colonies of rMSCs expanded into confluent cultures, whereas colonies of hMSCs did not...
  3. ncbi BMP-6 enhances chondrogenesis in a subpopulation of human marrow stromal cells
    I Sekiya
    Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, Louisiana 70112, USA
    Biochem Biophys Res Commun 284:411-8. 2001
    ..5-fold larger, stained more extensively for proteoglycans, and had levels of mRNA for type II procollagen that were 1.6-fold higher. Also, RS cells retained more of their chondrogenic potential as the cells were passaged...
  4. ncbi Plastic adherent stromal cells from the bone marrow of commonly used strains of inbred mice: variations in yield, growth, and differentiation
    D G Phinney
    Center for Gene Therapy, MCP Hahnemann University, Philadelphia, Pennsylvania 19102, USA
    J Cell Biochem 72:570-85. 1999
    ..Our results point to significant strain differences in the properties of MSCs and indicate that standard methods cannot be applied to murine bone marrow to isolate relatively pure populations of MSCs...
  5. ncbi Targeted disruption of Col11a2 produces a mild cartilage phenotype in transgenic mice: comparison with the human disorder otospondylomegaepiphyseal dysplasia (OSMED)
    S W Li
    Center for Gene Therapy, MCP Hahnemann University, Philadelphia, Pennsylvania, USA
    Dev Dyn 222:141-52. 2001
    ....
  6. ncbi Rat marrow stromal cells rapidly transduced with a self-inactivating retrovirus synthesize L-DOPA in vitro
    E J Schwarz
    Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, LA 70112, USA
    Gene Ther 8:1214-23. 2001
    ..Furthermore, two high-expressing clones were isolated and expanded at low-density from rMSCs transduced with pSIR driven by the PGK promoter (97.0% GFP+ or 1096.0 pmols L-DOPA/10(6) cells/h)...
  7. ncbi Correction of a mineralization defect by overexpression of a wild-type cDNA for COL1A1 in marrow stromal cells (MSCs) from a patient with osteogenesis imperfecta: a strategy for rescuing mutations that produce dominant-negative protein defects
    R R Pochampally
    Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, LA 70112, USA
    Gene Ther 12:1119-25. 2005
    ..The results indicated that it is possible to overexpress COL1A1 cDNA in OI MSCs and thereby to correct partially the dominant-negative protein defect...
  8. ncbi Identification of a subpopulation of rapidly self-renewing and multipotential adult stem cells in colonies of human marrow stromal cells
    D C Colter
    Center for Gene Therapy, Tulane University Health Sciences Center, 1430 Tulane Avenue SL-99, New Orleans, LA 70112, USA
    Proc Natl Acad Sci U S A 98:7841-5. 2001
    ..The results suggested it will be important to distinguish the major subpopulations of marrow stromal cells in defining their biology and their potential for cell and gene therapy...
  9. ncbi Isolation and characterization of rapidly self-renewing stem cells from cultures of human marrow stromal cells
    D J Prockop
    Center for Gene Therapy, Tulane University Health Sciences Center, 1430 Tulane Avenue, SL-99, New Orleans, LA 70112, USA
    Cytotherapy 3:393-6. 2001
    ..They have properties that appear to make them ideal candidates for studying differentiation and probably make them well-suited for cell and gene therapy...
  10. ncbi Stable transfection of MSCs by electroporation
    A Peister
    Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, LA 70112, USA
    Gene Ther 11:224-8. 2004
    ..The transfected MSCs retained their capacity to differentiate into both adipocytes and osteoblasts. Thus, MSCs were stably transfected with plasmid DNA and retained their differentiation capacity after expansion...
  11. ncbi Donor variation in the growth properties and osteogenic potential of human marrow stromal cells
    D G Phinney
    Center for Gene Therapy, MCP Hahnemann University, Philadelphia, Pennsylvania 19102, USA
    J Cell Biochem 75:424-36. 1999
    ..Therefore, it appears that cellular heterogeneity produced by the method of harvest is propagated within and among different donor populations during culture expansion in vitro...
  12. ncbi Engraftment and migration of human bone marrow stromal cells implanted in the brains of albino rats--similarities to astrocyte grafts
    S A Azizi
    Department of Neurology, Allegheny University of the Health Sciences, Broad and Vine Streets, Philadelphia, PA 19102, USA
    Proc Natl Acad Sci U S A 95:3908-13. 1998
    ..The results suggest that MSCs may be useful vehicles for autotransplantation in both cell and gene therapy for a variety of diseases of the central nervous system...
  13. ncbi Potential use of stem cells from bone marrow to repair the extracellular matrix and the central nervous system
    D J Prockop
    Center for Gene Therapy, MCP Hahnemann University, 10118 New College Building, 245 North 15 St, Philadelphia, PA 19102, USA
    Biochem Soc Trans 28:341-5. 2000
    ..The cells appear to be potentially useful for the repair of extracellular matrix and the central nervous system...
  14. ncbi In vitro differentiation of human marrow stromal cells into early progenitors of neural cells by conditions that increase intracellular cyclic AMP
    W Deng
    Center for Gene Therapy, Tulane University Health Sciences Center, SL-99, 1430 Tulane Avenue, New Orleans, Louisiana, 70112, USA
    Biochem Biophys Res Commun 282:148-52. 2001
    ..The data suggested that the hMSCs may have been differentiated into early progenitors of neural cells in vitro under conditions that increase the intracellular level of cAMP...
  15. ncbi Transgenic mice with inactive alleles for procollagen N-proteinase (ADAMTS-2) develop fragile skin and male sterility
    S W Li
    Center for Gene Therapy, MCP Hannemann University, Philadelphia, PA 19102, USA
    Biochem J 355:271-8. 2001
    ..Female homozygous mice were fertile, but male mice were sterile with a marked decrease in testicular sperm. Therefore the results indicated that ADAMTS-2 plays an essential role in the maturation of spermatogonia...
  16. ncbi Marrow stromal cells as a source of progenitor cells for nonhematopoietic tissues in transgenic mice with a phenotype of osteogenesis imperfecta
    R F Pereira
    Center for Gene Therapy, Allegheny University of the Health Sciences, Philadelphia, PA 19102 1192, USA
    Proc Natl Acad Sci U S A 95:1142-7. 1998
    ..The results support previous suggestions that marrow stromal cells or related cells in marrow serve as a source for continual renewal of cells in a number of nonhematopoietic tissues...
  17. ncbi Widely distributed mutations in the COL2A1 gene produce achondrogenesis type II/hypochondrogenesis
    J Korkko
    Center for Gene Therapy, MCP Hahnemann University, Philadelphia, Pennsylvania, USA
    Am J Med Genet 92:95-100. 2000
    ..They also demonstrate that most, if not all, patients with achondrogenesis type II/hypochondrogenesis have mutations in the COL2A1 gene...
  18. ncbi Lack of a phenotype in transgenic mice aberrantly expressing COL2A1 mRNA because of highly selective post-transcriptional down-regulation
    C M Yuan
    Center for Gene Therapy, MCP Hahnemann University, Philadelphia, PA 19102, USA
    Biochem J 345:377-84. 2000
    ....
  19. ncbi A potential role for Dkk-1 in the pathogenesis of osteosarcoma predicts novel diagnostic and treatment strategies
    N Lee
    Department of Medicine, Center for Gene Therapy, Tulane University Health Sciences Center, 1430 Tulane Avenue, New Orleans, LA 70112, USA
    Br J Cancer 97:1552-9. 2007
    ..These data demonstrate that Dkk-1 may serve as a prognostic or diagnostic marker for evaluation of OS and furthermore, immunodepletion of Dkk-1 or administration of GSK3beta inhibitors could represent an adjunct therapy for this disease...