Research Topics
| Garth EhrlichSummaryAffiliation: Allegheny General Hospital Country: USA Publications
Research Grants
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Detail Information
Publications
Preliminary study: treatment with intramuscular interferon beta-1a results in increased levels of IL-12Rβ2+ and decreased levels of IL23R+ CD4+ T - Lymphocytes in multiple sclerosisJennifer M Kress-Bennett
Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, PA 15212, USA
BMC Neurol 11:155. 2011....- Fibroblasts from phenotypically normal palmar fascia exhibit molecular profiles highly similar to fibroblasts from active disease in Dupuytren's ContractureLatha Satish
Department of Surgery, Division of Plastic Surgery, University of Pittsburgh Medical Center, Pittsburgh, PA, USA
BMC Med Genomics 5:15. 2012.... - Structure and dynamics of the pan-genome of Streptococcus pneumoniae and closely related speciesClaudio Donati
Novartis Vaccines and Diagnostics, Via Fiorentina 1, 53100 Siena, Italy
Genome Biol 11:R107. 2010..Streptococcus pneumoniae is one of the most important causes of microbial diseases in humans. The genomes of 44 diverse strains of S. pneumoniae were analyzed and compared with strains of non-pathogenic streptococci of the Mitis group... - Characterization and modeling of the Haemophilus influenzae core and supragenomes based on the complete genomic sequences of Rd and 12 clinical nontypeable strainsJustin S Hogg
Allegheny General Hospital, Allegheny Singer Research Institute, Center for Genomic Sciences, Pittsburgh, Pennsylvania 15212, USA
Genome Biol 8:R103. 2007..Studies based on random sequencing of multiple strain libraries suggested that free-living bacterial species possess a supragenome that is much larger than the genome of any single bacterium... - Multiple displacement amplification as an adjunct to PCR-based detection of Staphylococcus aureus in synovial fluidSandeep Kathju
Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, Pittsburgh, PA, USA
BMC Res Notes 3:259. 2010..aureus DNA as well as on clinical samples known to contain S. aureus nucleic acids... - Physiology of Pseudomonas aeruginosa in biofilms as revealed by transcriptome analysisJames P Folsom
Center for Biofilm Engineering and Department of Chemical and Biological Engineering, PO Box 173980, Montana State University Bozeman, Bozeman, Montana 59717 3980, USA
BMC Microbiol 10:294. 2010.... - Characterization of biofilm matrix, degradation by DNase treatment and evidence of capsule downregulation in Streptococcus pneumoniae clinical isolatesLuanne Hall-Stoodley
Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, PA 15212, USA
BMC Microbiol 8:173. 2008..To better understand pneumococcal biofilm formation six low-passage encapsulated nasopharyngeal isolates of S. pneumoniae were assessed over a six-eight day period in vitro... - Virulence phenotypes of low-passage clinical isolates of nontypeable Haemophilus influenzae assessed using the chinchilla laniger model of otitis mediaFarrel J Buchinsky
Center for Genomic Sciences, Allegheny Singer Research Institute Allegheny General Hospital, Pittsburgh, Pennsylvania 15212, USA
BMC Microbiol 7:56. 2007..These diverse clinical and genotypic findings suggest that each NTHi strain possesses a unique set of virulence factors that contributes to the course of the disease... - Comparative supragenomic analyses among the pathogens Staphylococcus aureus, Streptococcus pneumoniae, and Haemophilus influenzae using a modification of the finite supragenome modelRobert Boissy
Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, PA 15212, USA
BMC Genomics 12:187. 2011.... - Comparative analysis and supragenome modeling of twelve Moraxella catarrhalis clinical isolatesJeremiah J Davie
Department of Microbiology and Immunology, University at Buffalo, Buffalo, New York, USA
BMC Genomics 12:70. 2011..catarrhalis clinical isolates, a greater understanding of this pathogen's genome and its variability among isolates is needed... 
Mucosal biofilm formation on middle-ear mucosa in the chinchilla model of otitis mediaGarth D Ehrlich
Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, PA 15212, USA
JAMA 287:1710-5. 2002....- What makes pathogens pathogenicGarth D Ehrlich
Center for Genomic Sciences, Allegheny General Hospital, Allegheny Singer Research Institute, Pittsburgh, PA 15212, USA
Genome Biol 9:225. 2008..This transition is likely to depend both on the prevailing environmental conditions and on specific gene-gene interactions placed within the context of the entire ecosystem... - Direct detection of bacterial biofilms on the middle-ear mucosa of children with chronic otitis mediaLuanne Hall-Stoodley
Center for Genomic Sciences, Allegheny-Singer Research Institute, Pittsburgh, PA 15212, USA
JAMA 296:202-11. 2006..CONCLUSION: Direct detection of biofilms on MEM biopsy specimens from children with OME and recurrent OM supports the hypothesis that these chronic middle-ear disorders are biofilm-related... - Strain-specific virulence phenotypes of Streptococcus pneumoniae assessed using the Chinchilla laniger model of otitis mediaMichael L Forbes
Department of Pediatrics, Allegheny General Hospital, Pittsburgh, Pennsylvania, United States of America
PLoS ONE 3:e1969. 2008.... 
Population-level virulence factors amongst pathogenic bacteria: relation to infection outcomeFen Ze Hu
Center for Genomic Sciences, Allegheny Singer Research Institute Allegheny General Hospital, USA
Future Microbiol 3:31-42. 2008..The recent recognition of multicellularity among chronic bacterial pathogens will lead the way towards new multimodality therapies...- Human susceptibility to viral infection: the search for HIV-protective alleles among Africans by means of genome-wide studiesJoseph Donfack
Center for Genomic Sciences, Allegheny Singer Research Institute, Drexel University College of Medicine, Pittsburgh, Pennsylvania 15212, USA
AIDS Res Hum Retroviruses 22:925-30. 2006.... - Identification of differentially expressed genes in scarless wound healing utilizing polymerase chain reaction-suppression subtractive hybridizationSandeep Kathju
Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, Pittsburgh, Pennsylvania 15212 4772, USA
Wound Repair Regen 14:413-20. 2006..With this approach, we have also identified novel genes potentially involved in scarless wound healing... - Genetics of pediatric gastroesophageal refluxJ Christopher Post
Center for Genomic Sciences, Allegheny Singer Research Institute, Drexel University College of Medicine, Philadelphia, Pennsylvania, USA
Curr Opin Allergy Clin Immunol 5:5-9. 2005..The results of family and twin studies have identified an inherited tendency toward developing the disease, and a locus common to multiple families with severe GERD has been found on 13q14, known as GERD1... - The role of biofilms in otolaryngologic infections: update 2007J Christopher Post
Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, Pennsylvania, USA
Curr Opin Otolaryngol Head Neck Surg 15:347-51. 2007..Biofilms have been shown to play a role in otitis media, sinusitis, cholesteatoma, tonsillitis, adenoiditis, and device infections. This article is written to review recent advances in the field... - Chronic surgical site infection due to suture-associated polymicrobial biofilmSandeep Kathju
Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, Pittsburgh, Pennsylvania 15212, USA
Surg Infect (Larchmt) 10:457-61. 2009..Surgical site infection (SSI) is a common surgical complication; culture-negative SSI presents a particular problem in management... - Multicenter initiative seeking critical genes in respiratory papillomatosisFarrel J Buchinsky
Center for Genomic Sciences, Allegheny Singer Research Institute, 320 E North Avenue, Pittsburgh, PA 15212 4772, USA
Laryngoscope 114:349-57. 2004..Genetically encoded host susceptibility is postulated. Determining the host genes that govern susceptibility will enhance our understanding not only of RRP but also of host-viral interaction in general... - Development and characterization of a pooled Haemophilus influenzae genomic library for the evaluation of gene expression changes associated with mucosal biofilm formation in otitis mediaGeza Erdos
Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, 320 E North Ave, Pittsburgh, PA 15212, USA
Int J Pediatr Otorhinolaryngol 67:749-55. 2003..It has been etiologically associated with otitis media, otorrhea, and chronic obstructive pulmonary disease. Identification of new genomic elements will provide novel targets to fight chronic infections caused by this organism... - New methods for the detection of orthopedic and other biofilm infectionsJohn William Costerton
Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, PA, USA
FEMS Immunol Med Microbiol 61:133-40. 2011.... - Fluorescence "in situ" hybridization for the detection of biofilm in the middle ear and upper respiratory tract mucosaLaura Nistico
Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, Pittsburgh, PA, USA
Methods Mol Biol 493:191-213. 2009.... - Expression of receptor for activated C kinase 1 in healing skin and mucosal woundsLatha Satish
Wound Healing Program, Center for Genomic Sciences, Allegheny Singer Research Institute, 320 East North Ave, Pittsburgh, PA 15212, USA
Ann Plast Surg 64:238-41. 2010..These observations suggest that RACK1 may play an important role in distinguishing scarless fetal wound healing from its scirrhous counterpart in adults... - The distributed genome hypothesis as a rubric for understanding evolution in situ during chronic bacterial biofilm infectious processesGarth D Ehrlich
Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, PA, USA
FEMS Immunol Med Microbiol 59:269-79. 2010.... - Fine mapping a gene for pediatric gastroesophageal reflux on human chromosome 13q14Fen Ze Hu
Center for Genomic Sciences, Allegheny Singer Research Institute, 320 East North Ave, Pittsburgh, PA 15212, USA
Hum Genet 114:562-72. 2004..The results suggest that the GERD1 gene might be located near SNP160 or SNP168... - Differential expression of chaperonin containing T-complex polypeptide (CCT) subunits during fetal and adult skin wound healingLatha Satish
Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, Pittsburgh, PA 15212, USA
Cell Stress Chaperones 13:527-33. 2008..We also confirm that quantitative evaluation of CCT-alpha and CCT-zeta mRNA expression shows no change in healing fetal wounds... - Identification, distribution, and expression of novel genes in 10 clinical isolates of nontypeable Haemophilus influenzaeKai Shen
Center for Genomic Sciences, Allegheny-Singer Research Institute, Allegheny General Hospital, 320 East North Ave, 11th Floor South Tower, Pittsburgh, PA 15212, USA
Infect Immun 73:3479-91. 2005..These genic distribution analyses were more effective as a strain discrimination tool than either multilocus sequence typing or 23S ribosomal gene typing methods... - Characterization, distribution, and expression of novel genes among eight clinical isolates of Streptococcus pneumoniaeKai Shen
Center for Genomic Sciences, Allegheny-Singer Research Institute, Allegheny General Hospital, 320 East North Avenue, 11th Floor South Tower, Pittsburgh, PA 15212, USA
Infect Immun 74:321-30. 2006..Sixty-five percent (38/58) of these sequences were found in 50% or less of the clinical strains, indicating a significant degree of genomic plasticity among natural isolates... - Codon usage comparison of novel genes in clinical isolates of Haemophilus influenzaeJohn Gladitz
Center for Genomic Sciences, Allegheny-Singer Research Institute Pittsburgh, PA, USA
Nucleic Acids Res 33:3644-58. 2005..The remaining novel sequences produced similarity statistics closer to one of the other reference genomes thereby suggesting that these sequences may have entered the H.influenzae gene pool more recently via horizontal transfer... - Direct demonstration of viable Staphylococcus aureus biofilms in an infected total joint arthroplasty. A case reportPaul Stoodley
Center for Genomic Sciences, Allegheny-Singer Research Institute, Allegheny General Hospital, 320 East North Avenue, Pittsburgh, PA 15212-4772, USA
J Bone Joint Surg Am 90:1751-8. 2008 - Construction and characterization of a highly redundant Pseudomonas aeruginosa genomic library prepared from 12 clinical isolates: application to studies of gene distribution among populationsGeza Erdos
Center for Genomic Sciences, Allegheny-Singer Research Institute, 320 E. North Avenue, Pittsburgh, PA 15212, United States
Int J Pediatr Otorhinolaryngol 70:1891-900. 2006..aeruginosa population-based supra-genome... - The role of biofilms in otolaryngologic infectionsJ Christopher Post
Center for Genomic Sciences, Allegheny Singer Research Institute, Pittsburgh, Pennsylvania 15212, USA
Curr Opin Otolaryngol Head Neck Surg 12:185-90. 2004..Understanding that many of the infections that affect structures of the head and neck are actually biofilm related is fundamental to developing rational strategies for treatment and prevention... - Extensive genomic plasticity in Pseudomonas aeruginosa revealed by identification and distribution studies of novel genes among clinical isolatesKai Shen
Center for Genomic Sciences, Allegheny-Singer Research Institute, Allegheny General Hospital, 320 East North Ave, 11th Floor South Tower, Pittsburgh, PA 15212, USA
Infect Immun 74:5272-83. 2006..Expression profiling revealed that a vast majority of these sequences are expressed, strongly suggesting they encode functional proteins... - Mucosal biofilm formation on middle-ear mucosa in a nonhuman primate model of chronic suppurative otitis mediaJoseph E Dohar
Department of Pediatric Otolaryngology, Children s Hospital of Pittsburgh, 3705 5th Avenue, Pittsburgh, PA 15213, USA
Laryngoscope 115:1469-72. 2005..Perhaps the best studied pathogen for its propensity toward biofilm formation is Pseudomonas aeruginosa, also the primary pathogen in chronic suppurative otitis media (CSOM)... - Comparative genomic analyses of seventeen Streptococcus pneumoniae strains: insights into the pneumococcal supragenomeN Luisa Hiller
Allegheny General Hospital, Allegheny Singer Research Institute, Center for Genomic Sciences, Pittsburgh, PA 15212, USA
J Bacteriol 189:8186-95. 2007.... - Four mutations in Epidermodysplasia verruciformis 1 (EVER1) gene are not contributors to susceptibility in RRPJoseph Donfack
Allegheny General Hospital, Allegheny Singer Research Institute, 320 East North Avenue, 11th Floor, South Tower, Room 1171, Pittsburgh, PA 15212 4772, USA
Int J Pediatr Otorhinolaryngol 70:1235-40. 2006.... - Biofilm formation by ica-positive and ica-negative strains of Staphylococcus epidermidis in vitroBethany Dice
Center for Genomic Sciences, Allegheny Singer Research Institute, Allegheny General Hospital, Pittsburgh, Pennsylvania, USA
Biofouling 25:367-75. 2009..It was concluded that ica is not required for biofilm formation, nor is the 24-h CV assay generalizable for predicting the 6-day biofilm-forming ability for all S. epidermidis strains... - Age of child, more than HPV type, is associated with clinical course in recurrent respiratory papillomatosisFarrel J Buchinsky
Allegheny General Hospital, Allegheny Singer Research Institute, Center for Genomic Sciences, Pittsburgh, Pennsylvania, United States of America
PLoS ONE 3:e2263. 2008..These data suggest that there are factors other than HPV type and age of the patient that determine disease course... - Unique challenges of obtaining regulatory approval for a multicenter protocol to study the genetics of RRP and suggested remediesMylaina L Sherwood
Center for Genomic Sciences at Allegheny Singer Research Institute, Allegheny General Hospital, 320 East North Avenue, Pittsburgh, PA 15212-4772, USA
Otolaryngol Head Neck Surg 135:189-96. 2006..For multicenter studies, investigators should seriously consider the establishment of cooperative authorization agreements. On a simpler level, a standardized format for applications needs to be adopted nationwide. EBM rating: B-3b... - Role of biofilms in neurosurgical device-related infectionsErnest E Braxton
Department of Neurosurgery, Allegheny General Hospital, Pittsburgh, PA, USA
Neurosurg Rev 28:249-55. 2005..Understanding how these biofilm infections affect patients with neurosurgical devices is a prerequisite to developing strategies for their treatment and prevention... - Phenotypic characterization of Streptococcus pneumoniae biofilm developmentMagee Allegrucci
State University of New York at Binghamton, Department of Biological Sciences, 104 Science III, Vestal Parkway East, Binghamton, NY 13902, USA
J Bacteriol 188:2325-35. 2006..Protein identification revealed that proteins involved in virulence, adhesion, and resistance were more abundant under biofilm growth conditions. A possible role of the identified proteins in biofilm formation is discussed... - Lack of major involvement of human uroplakin genes in vesicoureteral reflux: implications for disease heterogeneitySongshan Jiang
Epithelial Biology Unit, Ronald O Perelman Department of Dermatology, Kaplan Comprehensive Cancer Center, New York University Medical School, New York, New York, USA
Kidney Int 66:10-9. 2004..Recent studies indicate that genetic ablation of mouse uroplakin (UP) III gene, which encodes a 47 kD urothelial-specific integral membrane protein forming urothelial plaques, causes VUR and hydronephrosis... - Recent advances in otitis media. 4A. Molecular biologyAllen F Ryan
Division of Otolaryngology, University of California, San Diego, La Jolla, USA
Ann Otol Rhinol Laryngol Suppl 194:42-9. 2005 - Efficacy of single-dose azithromycin in treatment of acute otitis media in children after a baseline tympanocentesisMichael W Dunne
Pfizer Global Research and Development, New London, Connecticut 06320, USA
Antimicrob Agents Chemother 47:2663-5. 2003..Clinical cure rates for patients infected with Streptococcus pneumoniae (67 of 76; 88%) and Haemophilus influenzae (28 of 44; 64%) were consistent with historical rates for the 5-day dosing regimen... - Recent advances in otitis media. 4C. Interaction between middle ear and inner ear in otitis mediaAllen F Ryan
Division of Otolaryngology, University of California, San Diego, La Jolla, USA
Ann Otol Rhinol Laryngol Suppl 194:56-9. 2005 - Arginine or nitrate enhances antibiotic susceptibility of Pseudomonas aeruginosa in biofilmsGiorgia Borriello
Center for Biofilm Engineering and Department of Clinical Engineering, Montana State University--Bozeman, 409 Cobleigh Hall, Bozeman, Montana 59717-3980, USA
Antimicrob Agents Chemother 50:382-4. 2006..Arginine or nitrate also enhanced the killing by these antibiotics in mature biofilms, reducing viable cell counts by a factor of 10 to 100 beyond that achieved by antibiotics alone... - Oxygen limitation contributes to antibiotic tolerance of Pseudomonas aeruginosa in biofilmsGiorgia Borriello
Center for Biofilm Engineering and Department of Chemical Engineering, Montana State University-Bozeman, Bozeman, MT 59717-3980, USA
Antimicrob Agents Chemother 48:2659-64. 2004..Local oxygen limitation and the presence of nitrate may contribute to the reduced susceptibilities of P. aeruginosa biofilms causing infections in vivo... - Biofilms and chronic infectionsRandall D Wolcott
Medical Biofilm Research Institute, 2002 Oxford Ave, Lubbock, TX 79410, USA
JAMA 299:2682-4. 2008 - Pseudomonas aeruginosa displays multiple phenotypes during development as a biofilmKarin Sauer
Center for Biofilm Engineering, Montana State University-Bozeman, Bozeman, Montana, USA
J Bacteriol 184:1140-54. 2002..These results demonstrate that P. aeruginosa displays multiple phenotypes during biofilm development and that knowledge of stage-specific physiology may be important in detecting and controlling biofilm growth... - Insights into the genome of large sulfur bacteria revealed by analysis of single filamentsMarc Mussmann
Max Planck Institute for Marine Microbiology, Bremen, Germany
PLoS Biol 5:e230. 2007..The first look into the genome of these filamentous sulfur-oxidizing bacteria substantially deepens the understanding of their evolution and their contribution to sulfur and nitrogen cycling in marine sediments...
Research Grants
- The Role of Biofilms in the Pathogenesis of OtorrheaGarth Ehrlich; Fiscal Year: 2007..A discovery that has immediate clinical relevancy is that biofilm antibiotic resistance is mediated largely by bacterial metabolic resistance owing to limiting nutrient conditions within the core of the biofilms. ..
- Impact of Antibiotics and Vaccines on the in vivo Evolution of S. pneumoniaeGarth Ehrlich; Fiscal Year: 2009..pneumoniae. The research program described at Allegheny Singer Research Institute (ASRI) headed by Garth Ehrlich, Roche 454 LifeSciences Titanium platform...
- Impact of Antibiotics and Vaccines on the in vivo Evolution of S. pneumoniaeGarth D Ehrlich; Fiscal Year: 2010..WGS will be used to test if the emergence of these strains from minority to majority status in the nasopharynx and from colonizers to disease causing strains is accompanied by changes in genetic makeup ..
- GENETICS OF SEVERE PEDIATRIC GASTROESOPHAGEAL REFLUXGarth Ehrlich; Fiscal Year: 2003....
- MOLECULAR ANALYSIS OF PATHOGENS IN OTITIS MEDIA BY PCRGarth Ehrlich; Fiscal Year: 2002..A combined final series of experiments will attempt to determine the mechanism(s) that regulate or trigger the formation of biofilms and to correlate different models of mutational2 induction with the consequences of antibiotic therapy. ..
- THE ROLE OF BIOFILMS IN THE PATHOGENESIS OF OTORRHEAGarth Ehrlich; Fiscal Year: 2002....
- MOLECULAR ANALYSIS OF PATHOGENS IN OTITIS MEDIA BY PCRGarth Ehrlich; Fiscal Year: 2009..These findings shed light on many aspects of OM, including disease persistence in the fact of antibiotic treatment, and provide an explanation for the success of adenoidectomy in the management of OM. ..