Jeffrey Wyckoff

Summary

Affiliation: Albert Einstein College of Medicine
Country: USA

Publications

  1. pmc Gene expression analysis on small numbers of invasive cells collected by chemotaxis from primary mammary tumors of the mouse
    Weigang Wang
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    BMC Biotechnol 3:13. 2003
  2. pmc Simultaneous imaging of GFP, CFP and collagen in tumors in vivo using multiphoton microscopy
    Erik Sahai
    Tumour Cell Biology Laboratory, Cancer Research UK London Research Institute, London WC2A 3PX, UK
    BMC Biotechnol 5:14. 2005
  3. ncbi request reprint Direct visualization of macrophage-assisted tumor cell intravasation in mammary tumors
    Jeffrey B Wyckoff
    Gruss Lipper Center for Biophotonics, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Cancer Res 67:2649-56. 2007
  4. ncbi request reprint A paracrine loop between tumor cells and macrophages is required for tumor cell migration in mammary tumors
    Jeffrey Wyckoff
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Cancer Res 64:7022-9. 2004
  5. pmc The activity status of cofilin is directly related to invasion, intravasation, and metastasis of mammary tumors
    Weigang Wang
    Department of Anatomy and Structural Biology and 2Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Cell Biol 173:395-404. 2006
  6. ncbi request reprint Imaging tumor cell movement in vivo
    Dmitriy Kedrin
    Albert Einstein College of Medicine, Bronx, New York, USA
    Curr Protoc Cell Biol . 2007
  7. pmc Contribution of CXCL12 secretion to invasion of breast cancer cells
    Pamela J Boimel
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1301 Morris Park Ave, Bronx, NY, 10461, USA
    Breast Cancer Res 14:R23. 2012
  8. ncbi request reprint ErbB3-dependent motility and intravasation in breast cancer metastasis
    Chengsen Xue
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10801, USA
    Cancer Res 66:1418-26. 2006
  9. pmc ERBB1 and ERBB2 have distinct functions in tumor cell invasion and intravasation
    Dmitriy Kedrin
    Department of Anatomy and Structural Biology and Gruss Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, New York, USA
    Clin Cancer Res 15:3733-9. 2009
  10. ncbi request reprint Probing the microenvironment of mammary tumors using multiphoton microscopy
    Mazen Sidani
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    J Mammary Gland Biol Neoplasia 11:151-63. 2006

Collaborators

Detail Information

Publications34

  1. pmc Gene expression analysis on small numbers of invasive cells collected by chemotaxis from primary mammary tumors of the mouse
    Weigang Wang
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    BMC Biotechnol 3:13. 2003
    ..We have combined chemotaxis-based cell collection of the invasive subpopulation of cells within the primary tumor with array-based gene expression analysis to identify the genes necessary for the process of carcinoma cell invasion...
  2. pmc Simultaneous imaging of GFP, CFP and collagen in tumors in vivo using multiphoton microscopy
    Erik Sahai
    Tumour Cell Biology Laboratory, Cancer Research UK London Research Institute, London WC2A 3PX, UK
    BMC Biotechnol 5:14. 2005
    ..However, the use of genetically encoded fluorescent proteins to distinguish different cell types in living animals has not been described at single cell resolution using multiphoton microscopy...
  3. ncbi request reprint Direct visualization of macrophage-assisted tumor cell intravasation in mammary tumors
    Jeffrey B Wyckoff
    Gruss Lipper Center for Biophotonics, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Cancer Res 67:2649-56. 2007
    ..These results show that the interaction between macrophages and tumor cells lying in close proximity defines a microenvironment that is directly involved in the intravasation of cancer cells in mammary tumors...
  4. ncbi request reprint A paracrine loop between tumor cells and macrophages is required for tumor cell migration in mammary tumors
    Jeffrey Wyckoff
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Cancer Res 64:7022-9. 2004
    ..This work provides the first direct evidence for a synergistic interaction between macrophages and tumor cells during cell migration in vivo and indicates a mechanism for how macrophages may contribute to metastasis...
  5. pmc The activity status of cofilin is directly related to invasion, intravasation, and metastasis of mammary tumors
    Weigang Wang
    Department of Anatomy and Structural Biology and 2Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Cell Biol 173:395-404. 2006
    ..LIMK1-mediated decreases or increases in the activity of the cofilin pathway are shown to cause proportional decreases or increases in motility, intravasation, and metastasis of tumor cells...
  6. ncbi request reprint Imaging tumor cell movement in vivo
    Dmitriy Kedrin
    Albert Einstein College of Medicine, Bronx, New York, USA
    Curr Protoc Cell Biol . 2007
    ..The Basic Protocol describes the standard methods used for generation of mammary tumors and imaging them. Additional protocols for labeling macrophages, blood vessel imaging, and image analysis are also included...
  7. pmc Contribution of CXCL12 secretion to invasion of breast cancer cells
    Pamela J Boimel
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1301 Morris Park Ave, Bronx, NY, 10461, USA
    Breast Cancer Res 14:R23. 2012
    ..In this study, we examined the role of increased production of CXCL12 in tumor cell invasion and malignancy...
  8. ncbi request reprint ErbB3-dependent motility and intravasation in breast cancer metastasis
    Chengsen Xue
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10801, USA
    Cancer Res 66:1418-26. 2006
    ....
  9. pmc ERBB1 and ERBB2 have distinct functions in tumor cell invasion and intravasation
    Dmitriy Kedrin
    Department of Anatomy and Structural Biology and Gruss Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, New York, USA
    Clin Cancer Res 15:3733-9. 2009
    ..Our studies were designed to evaluate the effect of ERBB inhibition on tumor cell motility and intravasation in vivo using clinically relevant small-molecule inhibitors...
  10. ncbi request reprint Probing the microenvironment of mammary tumors using multiphoton microscopy
    Mazen Sidani
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    J Mammary Gland Biol Neoplasia 11:151-63. 2006
    ..Results show that the tumor microenvironment is a dynamic place where interactions between tumor cells, macrophages, blood vessels, and extracellular matrix fibers define the metastatic phenotype...
  11. pmc Invasion of human breast cancer cells in vivo requires both paracrine and autocrine loops involving the colony-stimulating factor-1 receptor
    Antonia Patsialou
    Department of Anatomy, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Cancer Res 69:9498-506. 2009
    ..In particular, we show that the autocrine contribution to invasion is specifically amplified in vivo through a tumor microenvironment-induced upregulation of CSF-1R expression via the transforming growth factor-beta1...
  12. pmc The EGF/CSF-1 paracrine invasion loop can be triggered by heregulin beta1 and CXCL12
    Lorena Hernandez
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10801, USA
    Cancer Res 69:3221-7. 2009
    ..Our results identify a stromal/tumor interaction that acts as an engine underlying invasion induced by multiple ligands...
  13. pmc In vivo assay for tumor cell invasion
    Lorena Hernandez
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY, USA
    Methods Mol Biol 571:227-38. 2009
    ..This assay leads to a better understanding of the cell types and signaling involved in the tumor microenvironment, and has the potential to be applied to a variety of in vivo models...
  14. pmc Wiskott-Aldrich syndrome protein regulates leukocyte-dependent breast cancer metastasis
    Dan Ishihara
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Cell Rep 4:429-36. 2013
    ..Our findings suggest that WASp acts to support both the migration of TAMs and the production of EGF, which in concert promote breast tumor metastasis...
  15. pmc N-WASP-mediated invadopodium formation is involved in intravasation and lung metastasis of mammary tumors
    Bojana Gligorijevic
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Cell Sci 125:724-34. 2012
    ....
  16. pmc Differential enhancement of breast cancer cell motility and metastasis by helical and kinase domain mutations of class IA phosphoinositide 3-kinase
    Huan Pang
    Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Cancer Res 69:8868-76. 2009
    ..Our observations suggest that, when compared with kinase domain mutations in a genetically identical background, expression of helical domain mutants of p110alpha produce a more severe metastatic phenotype...
  17. ncbi request reprint GFP expression in the mammary gland for imaging of mammary tumor cells in transgenic mice
    Fayyaz Ahmed
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Cancer Res 62:7166-9. 2002
    ..These animals provide a range of expression of GFP that is suitable for analysis of transgenic mammary tumors and metastases in vivo at the single cell level of resolution...
  18. pmc Imaging tumor cell movement in vivo
    David Entenberg
    Albert Einstein College of Medicine, Bronx, New York, USA
    Curr Protoc Cell Biol . 2013
    ..The Basic Protocol in this unit describes the standard methods used for generation of mammary tumors and imaging them. Additional protocols for labeling macrophages, blood vessel imaging, and image analysis are also included...
  19. ncbi request reprint Epidermal growth factor receptor overexpression results in increased tumor cell motility in vivo coordinately with enhanced intravasation and metastasis
    Chengsen Xue
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Cancer Res 66:192-7. 2006
    ....
  20. ncbi request reprint ZBP1 enhances cell polarity and reduces chemotaxis
    Kyle Lapidus
    Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461 1975, USA
    J Cell Sci 120:3173-8. 2007
    ..We found that ZBP1 expression actually reduced tumor cell motility and chemotaxis, presumably mediating their decreased metastatic potential by reducing their ability to respond to signals necessary for invasion...
  21. pmc The use of fluorescent proteins for intravital imaging of cancer cell invasion
    James Hulit
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY, USA
    Methods Mol Biol 872:15-30. 2012
    ..We provide procedures for generating tumors through injection of tumor cell lines, and multiphoton imaging using a skin-flap tumor dissection and a mammary imaging window...
  22. ncbi request reprint Single cell behavior in metastatic primary mammary tumors correlated with gene expression patterns revealed by molecular profiling
    Weigang Wang
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Cancer Res 62:6278-88. 2002
    ..We conclude that aligning cell behavior in vivo with patterns of gene expression can lead to new insights into the microenvironment of carcinoma cells in the primary tumor and the molecular mechanisms behind cell behavior...
  23. pmc Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imaging
    David Entenberg
    Department of Anatomy and Structural Biology, and Gruss Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, New York, USA
    Nat Protoc 6:1500-20. 2011
    ..We demonstrate the use of the setup and plug-in by presenting data collected via intravital imaging of a mouse model of breast cancer. The procedure may be completed in ∼24 h...
  24. pmc Intravital imaging of metastatic behavior through a mammary imaging window
    Dmitriy Kedrin
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine of Yeshiva University, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Nat Methods 5:1019-21. 2008
    ..We found that two distinct microenvironments in the same orthotopic mammary tumor affected differently the invasion and intravasation of tumor cells...
  25. ncbi request reprint Coordinated regulation of pathways for enhanced cell motility and chemotaxis is conserved in rat and mouse mammary tumors
    Weigang Wang
    Department of Anatomy and Structural Biology and Gruss Lipper Center for Biophotonics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Cancer Res 67:3505-11. 2007
    ....
  26. ncbi request reprint Tumor cells caught in the act of invading: their strategy for enhanced cell motility
    Weigang Wang
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Trends Cell Biol 15:138-45. 2005
    ..These results support a "tumor microenvironment invasion model" and provide new target opportunities for cancer therapy...
  27. pmc Mena deficiency delays tumor progression and decreases metastasis in polyoma middle-T transgenic mouse mammary tumors
    Evanthia T Roussos
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Breast Cancer Res 12:R101. 2010
    ..Whether Mena is required for tumor progression is still unknown. Here we report the effects of Mena deficiency on tumor progression, metastasis and on normal mammary gland development...
  28. ncbi request reprint Macrophages promote the invasion of breast carcinoma cells via a colony-stimulating factor-1/epidermal growth factor paracrine loop
    Sumanta Goswami
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Cancer Res 65:5278-83. 2005
    ..Disruption of this loop by blockade of either EGF receptor or CSF-1 receptor signaling is sufficient to inhibit both macrophage and tumor cell migration and invasion...
  29. ncbi request reprint Identification and testing of a gene expression signature of invasive carcinoma cells within primary mammary tumors
    Weigang Wang
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Cancer Res 64:8585-94. 2004
    ..ZBP1 reexpression also inhibited metastasis from tumors. These experiments support the involvement in metastasis of the pathways identified in invasive cells, which are regulated by ZBP1...
  30. ncbi request reprint Breast cancer cells isolated by chemotaxis from primary tumors show increased survival and resistance to chemotherapy
    Sumanta Goswami
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Cancer Res 64:7664-7. 2004
    ..In addition, these results indicate that tumor cells that are chemotactic and migratory in response to epidermal growth factor in the primary tumor have a survival advantage over stationary tumor cells...
  31. ncbi request reprint Description and characterization of a chamber for viewing and quantifying cancer cell chemotaxis
    Lilian Soon
    Albert Einstein College of Medicine, Jack and Pearl Resnick Campus, Bronx, New York, USA
    Cell Motil Cytoskeleton 62:27-34. 2005
    ..This result suggests that polarized cancer cells can avoid the need for oscillatory path corrections during chemotaxis...
  32. ncbi request reprint Cell migration in tumors
    Hideki Yamaguchi
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Curr Opin Cell Biol 17:559-64. 2005
    ..These results, combined with new findings from in vitro studies, have led to new insights into the molecular mechanisms of cell protrusive activity and chemotactic migration during invasion and metastasis...
  33. ncbi request reprint ROCK- and myosin-dependent matrix deformation enables protease-independent tumor-cell invasion in vivo
    Jeffrey B Wyckoff
    Analytical Imaging Facility, Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Curr Biol 16:1515-23. 2006
    ..Imaging of live tumors shows that MLC is organized in a similar ROCK-dependent fashion in vivo and that inhibition of ROCK but not matrix-metalloproteases reduces cancer cell motility in vivo...
  34. pmc Caveolin-1 knockout mice show an impaired angiogenic response to exogenous stimuli
    Scott E Woodman
    Department of Molecular Pharmacology, Einstein Cancer Center, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Am J Pathol 162:2059-68. 2003
    ..These results provide novel evidence that Cav-1 and caveolae play an important positive role in the process of pathological angiogenesis in vivo...